LOCUS       MR209864                6870 bp    DNA     circular  SYN 
DEFINITION  Arhgap18 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 18 (Arhgap18)
ACCESSION   NM_176837
VERSION     NM_176837.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3027..3118 
                     /label="Myc-DDK"  
     misc_feature    3148..3733 
                     /label="PolyA signal"  
     misc_feature    3205..3224 
                     /label="Restriction Enzyme"  
     misc_feature    4009..4681 
                     /label="ColE1"  
     misc_feature    5111..5905 
                     /label="Kan/Neo"  
     misc_feature    5940..6242 
                     /label="SV40 ori"  
     misc_feature    6328..6805 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3009..3015 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3009 
                     /label="Arhgap18"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACTGGCTA TCGAGTTCCT CGGGAGTTGT GCTAACTGCT TACCACCCCA 
     1081 GCGGCAAGGA CCAGGTCGCT GGGGACAGCC ATGTAAAGGG AGGCGACGAA GCCACCTCCA 
     1141 GTCGCAGATA TGGCCAATAC ACCATAAACC AAGAAGGCTC CACCAAAGTT CCCGAGAGGC 
     1201 CTCCATTTGA TCGATCGAGT TCTCAGGATT CCTTGGATGA ATCAATGGAA GCCTATTGGA 
     1261 CAGAACTAGA AAACATCAAG AGGTCGAATG AAAATCGCCA AGAAGGTCAA GAGGCCATAG 
     1321 TTGTCAAAGA ACCTGATGAG GGAGAACTGG AAGAAGAATG GCTCAAAGAA GCAGGTCTAT 
     1381 CCAATCTTTT TGGAGAGTCT ATTGATGACC CGCAGGAAAG CATTCTGTTT TTATCTACAC 
     1441 TGACCCGGAC TCAGGCAGCA GCTGTTCAGA AACGGGTGGA AACGGTCTCC CAGACCTTGA 
     1501 GGAAGAAAAA CAAACAGCAC CACATTCGTG ACGTCAGAGA CATATTTGCT CAACAGAGAG 
     1561 AGGCACAAGA AAAACCTCCA GATGACTCAG ACTTGCGGTC AGTCAGAACA AACGAAAACA 
     1621 AAGGCCAAGG AAAAGATGAT CAGCCATCTA GTGGGGCTGT CGATAGCAAG GAGCAGATCT 
     1681 CACGGGTGCC TGAGGACACA CCTGCCTCTG AAACAGACAT CAATCTGGAG GTGTCATTTG 
     1741 CGGAGCAAGC TGTCAATCAG AAAGAGTTCA GTAAGGAGAG GACGCAGAAG ATCAGCAGCA 
     1801 ACGACTCTTT GCCCAGTTTC AGACTGCCAA AAGATAAAAC AGGTACGACA AGGATTGGAG 
     1861 ACTTGGCACC CCAAGACATG AAGAAAGTTT GCTCTCTATC CCTGATTGAA CTCACTGCCC 
     1921 TGTATGATGT GTTGGGCTTG GAATTCAAAC AACAGAAAGC TGTGAAAATC AAAACCAGAG 
     1981 ACTCTGGTCT TTTTGGTATT CCTTTAACGA TATTGTTAGA ACAAGATCAA AGAAAAGTGC 
     2041 CTGGAACACG AATACCCTTG ATCTTTCAAA AACTGATTTC TCGAATTGAA GAAGGCAGTC 
     2101 TGGAAACCGA GGGCCTCCTA CGGATACCAG GAGCAGCCAT GAGAATCAAG AATCTTTGCC 
     2161 AAGAACTAGA AGCGAAATTT TACGAGGGGA CTTTCAATTG GGAAAGTGTC AAACAGCACG 
     2221 ATGCGGCCAG CCTGCTGAAG CTCTTTCTGA GAGAATTGCC CCAGCCACTG CTGAGCATGG 
     2281 AGTATCTCAA AGCCTTCCAA GCTGTCCAGA ATCTTCCAAC CAGAAAGGAA CAGTTGCAGG 
     2341 CTTTGAACCT CCTTGTCATT CTTCTACCTG ACGCAAACAG AGACACACTG AAGGCCTTGC 
     2401 TTGAATTTCT CCAAAGAGTC ATAGATAACA AAGAGAAGAA CAAGATGACA GCTGGGAATG 
     2461 TAGCAATGGT CATGGCCCCA AATCTCTTCA TGTGCCACAC GCTGGGCTTG AAGTCCAGTG 
     2521 AACAGCGAGA ATTTGAAATG GCAGCTGGGA CAGCAAATGT CATGCACTTA TTGATTAGGT 
     2581 ACCAGAAAAT TCTATGGACA ATCCCTAAAT TTATTGTAAT CCAAGTGAGG AAACAGAATA 
     2641 TTGAGAATCA AAAGAAGGAA AGGAAAGCCA TGAAGAAATT GCTGAAGAAA ATGGCTTATG 
     2701 ACCGGGAAAA ACATGAAAAG CAAGATAAAA CTGCAAATGG TGCTGATGTT CCTCAGGGAG 
     2761 TGATTCGAGT GCAAGCTCCC CATCTTTCAA AAGTTTCCAT GGCAATACAG CTAACTGAAG 
     2821 AACTAAAAGC CAGCGATGTG CTTGCCAGGT TTCTCAGCCA AGAAAGTGGG GTTGCCCAGA 
     2881 CACTCAAGAA AGGAGAAGTT TTCCTGTATG AGATTGGAGG AAATATTGGG GAACGTTGCC 
     2941 TCGATGACGA CACACACATG AAGGACTTGT ACCAACTGAA CCCGAATGCC GAGTGGGTCA 
     3001 TAAAATCCAA GCCAGTGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3061 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3121 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3181 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     3241 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     3301 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     3361 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     3421 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     3481 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     3541 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     3601 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     3661 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     3721 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     3781 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     3841 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     3901 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     3961 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4021 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4081 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4141 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4201 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     4261 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     4321 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     4381 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     4441 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     4501 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     4561 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     4621 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     4681 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     4741 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     4801 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     4861 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     4921 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     4981 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5041 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5101 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5161 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     5221 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     5281 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     5341 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     5401 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     5461 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     5521 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     5581 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     5641 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     5701 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     5761 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     5821 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     5881 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     5941 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6001 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6061 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6121 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6181 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6241 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     6301 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     6361 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     6421 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     6481 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     6541 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     6601 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     6661 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     6721 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     6781 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     6841 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//