LOCUS       MR209863                6876 bp    DNA     circular  SYN 
DEFINITION  Dusp8 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 8 (Dusp8)
ACCESSION   NM_008748
VERSION     NM_008748.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3033..3124 
                     /label="Myc-DDK"  
     misc_feature    3154..3739 
                     /label="PolyA signal"  
     misc_feature    3211..3230 
                     /label="Restriction Enzyme"  
     misc_feature    4015..4687 
                     /label="ColE1"  
     misc_feature    5117..5911 
                     /label="Kan/Neo"  
     misc_feature    5946..6248 
                     /label="SV40 ori"  
     misc_feature    6334..6811 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3015..3021 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3015 
                     /label="Dusp8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGGGGAT CGGCTCCCGA GGAAGGTGAT GGACGCAAAG AAACTGGCCA 
     1081 GCCTGCTGCG TGGCGGGCCT GGGGGACCCT TGGTCATCGA CAGCCGGTCC TTCGTGGAGT 
     1141 ATAACAGCTG CCACGTGCTG AGCTCTGTGA ATATCTGCTG TTCAAAGCTG GTGAAGCGGC 
     1201 GCCTTCAGCA GGGAAAAGTG ACAATTGCTG AGCTTATCCA GCCTGCTACA CGGAGCCAGG 
     1261 TGGATGCCAC AGAACCACAG GATGTAGTGG TGTATGACCA GAGCACACGA GATGCCAGCG 
     1321 TGCTGGCAGC AGACAGCTTC CTGTCCATCC TGCTCAGCAA GCTGGACGGC TGCTTCGACA 
     1381 GTGTGGCCAT CCTCACAGGA GGCTTCGCCA CCTTCTCCTC CTGCTTCCCT GGCCTCTGTG 
     1441 AGGGCAAGCC TGCCACTCTA CCGTCCATGA GCCTCTCTCA GCCCTGCCTG CCTGTGCCCA 
     1501 GTGTTGGCCT GACCCGAATC CTGCCTCACC TCTACCTGGG CTCTCAGAAA GATGTCTTGA 
     1561 ACAAGGATCT GATGACCCAA AACGGAATAA GCTATGTCCT CAATGCCAGC AACTCCTGCC 
     1621 CTAAACCGGA CTTCATCTGT GAGAGCCGTT TCATGCGTAT CCCCATCAAT GACAACTACT 
     1681 GTGAAAAGCT GCTGCCCTGG CTGGACAAGT CCATCGAGTT TATTGATAAA GCCAAGCTGT 
     1741 CCAGCTGCCA AGTCATTGTT CACTGTCTGG CTGGCATCTC TCGCTCTGCC ACCATTGCCA 
     1801 TCGCGTACAT CATGAAAACC ATGGGCATGT CTTCTGACGA CGCATACAGG TTTGTGAAGG 
     1861 ATCGGCGCCC CTCCATCTCG CCCAACTTCA ACTTCCTGGG CCAGTTGCTG GAGTATGAGA 
     1921 GGAGTCTGAA GCTGCTGGCT GCCCTGCAGA CTGATGGACC TCACTTGGGG ACCCCTGAGC 
     1981 CCCTCATGGG CCCGGCAGCA GGCATCCCAC TGCCCCGGCT GCCACCATCT ACCTCAGAGA 
     2041 GCGCTGCCAC TGGGAGCGAG GCAGCCACCG CAGCCAGGGA GGGCAGCCCA AGTGCTGGAG 
     2101 GGGATGCTCC GATCCCCAGC ACAGCTCCAG CCACCAGCGC GCTGCAGCAG GGCCTGCGTG 
     2161 GCCTGCACCT CTCCTCTGAC CGCCTCCAGG ACACCAACCG CCTCAAGCGT TCCTTTTCCC 
     2221 TGGACATCAA GTCGGCCTAT GCACCCAGCA GGAGGCCCGA CTTTCCCGGC CCACCCGACC 
     2281 CCGGTGAAGC CCCGAAGCTC TGCAAGCTGG ACAGCCCGTC TGGGGGCACA CTGGGCCTGC 
     2341 CCTCGCCCAG CCCAGACAGC CCGGACTCCG TTCCAGAGTG CCGCCCACGA CCCCGCCGGC 
     2401 GACGCCCCCC GGCTAGTTCG CCTGCCCGCT CCCCCGCGCA TGGTCTGGGC CTGAACTTTG 
     2461 GAGACACGGC CCGGCAGACT CCACGGCACG GCCTCTCGGC CCTGTCGGCG CCCGGGCTGC 
     2521 CTGGCCCTGG CCAGCCGGCT GGCCCCGGGG GCTGGGTGCC GCCACTGGAC TCCCCAGGCA 
     2581 CACCGTCGCC CGACGGCCCC TGGTGCTTCA GCCCCGAGGG CGCGCAGGGT CCAGGCGCTG 
     2641 TGTTCTCCGC CTTTGGCCGG GTAAGTGCAG GCGCACCTGG ACCCGGTAAC AGCAGCAGCA 
     2701 GCGGTGGTGG TGGTGGTGGT GGTGGCGGCG GCGGCGGCGG CGGCGGCGGC GGCGGCAGCA 
     2761 GCAGCAGCAA CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGTA 
     2821 GTAGTAGTAG TAGTGACCTG CGGAGGCGGG ATGTGCGGAC CGGCTGGCCC GAGGAGCCTG 
     2881 CTGCAGATGC ACAGTTCAAG AGGCGCAGCT GCCAGATGGA GTTCGAAGAG GGCATGGTGG 
     2941 AGGGGCGGGC ACGTGGCGAG GAGCTGGCAG CCCTGGGCAA GCAAACCAGC TTCTCTGGCA 
     3001 GCGTGGAGGT CATCGAAGTA TCGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3061 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3121 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3181 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3241 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3301 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3361 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3421 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3481 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3541 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3601 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     3661 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     3721 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     3781 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     3841 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     3901 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     3961 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4021 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4081 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4141 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4201 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4261 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4321 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4381 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4441 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4501 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4561 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4621 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     4681 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     4741 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     4801 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     4861 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     4921 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     4981 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5041 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5101 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5161 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5221 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5281 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5341 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5401 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5461 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5521 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5581 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     5641 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     5701 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     5761 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     5821 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     5881 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     5941 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6001 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6061 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6121 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6181 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6241 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6301 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6361 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6421 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6481 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6541 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6601 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     6661 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     6721 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     6781 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     6841 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//