LOCUS       MG224702                9395 bp    DNA     circular  SYN 
DEFINITION  Dspp (tGFP-tagged) - Mouse dentin sialophosphoprotein (Dspp), (10ug). Note: ORF is codon optimized
ACCESSION   NM_010080
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3876..4580 
                     /label="GFP"  
     misc_feature    4627..5212 
                     /label="PolyA signal"  
     misc_feature    7304..7949 
                     /label="ColE1"  
     misc_feature    5666..6460 
                     /label="Neo"  
     misc_feature    5297..5599 
                     /label="SV40 ori"  
     misc_feature    7964..8824 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3855..3861 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3855 
                     /label="Dspp"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAATGAAA ATCATTATCT ATATTTGTAT CTGGGCAACC GCTTGGGCGA 
     1081 TCCCGGTCCC CCAGCTTGTA CCCCTGGAGC GCGACATTGT CGAAAACTCC GTGGCCGTAC 
     1141 CACTTCTGAC CCACCCAGGA ACGGCCGCAC AGAATGAGCT TTCCATAAAT TCCACCACTA 
     1201 GCAATAGCAA CGATTCCCCA GATGGTAGTG AGATTGGTGA ACAGGTACTT TCCGAGGACG 
     1261 GCTACAAGAG GGACGGAAAC GGATCTGAGT CCATACACGT GGGGGGTAAG GACTTTCCAA 
     1321 CCCAGCCTAT CCTGGTGAAT GAACAGGGGA ATACGGCAGA AGAACACAAC GATATTGAGA 
     1381 CGTATGGGCA TGACGGAGTC CACGCTCGCG GCGAGAATTC CACAGCGAAC GGTATACGGT 
     1441 CCCAGGTCGG AATTGTTGAG AATGCTGAAG AAGCCGAATC CTCCGTGCAT GGGCAGGCAG 
     1501 GTCAAAACAC CAAAAGCGGA GGAGCTAGTG ATGTATCCCA GAACGGAGAC GCAACGCTTG 
     1561 TTCAGGAGAA CGAACCCCCA GAGGCCAGCA TAAAGAATTC CACAAACCAC GAGGCTGGGA 
     1621 TCCACGGAAG CGGTGTCGCA ACACACGAGA CTACGCCCCA GCGCGAAGGA CTTGGGTCTG 
     1681 AGAATCAGGG GACTGAGGTT ACCCCAAGTA TCGGCGAGGA CGCGGGTCTG GATGATACCG 
     1741 ATGGGAGCCC TTCCGGCAAC GGGGTGGAGG AGGATGAAGA TACGGGCTCT GGCGATGGGG 
     1801 AGGGTGCCGA AGCGGGCGAC GGAAGGGAAT CTCACGACGG CACTAAAGGC CAAGGTGGGC 
     1861 AGTCCCACGG GGGCAATACC GATCACCGCG GGCAGAGTAG TGTATCTACT GAGGATGACG 
     1921 ACTCTAAAGA GCAGGAGGGT TTCCCGAATG GGCATAACGG CGACAATAGC TCCGAGGAGA 
     1981 ATGGGGTGGA GGAAGGGGAT AGCACTCAGG CCACCCAAGA TAACCAAAAG CTGAGTCCCA 
     2041 AAGATACTCG AGATGCCGAG GGGGGGATCA TCAGTCAGAG CGAAGCCTGT CCGTCTGGGA 
     2101 AATCTCAGGA TCAAGGCATT GAGACAGAGG GTCCGAATAA GGGCAACAAG AGCATTATTA 
     2161 CCAAAGAAAG TGGCAAGCTC TCCGGAAGTA AAGATAGCAA TGGCCATCAG GGCGTTGAGC 
     2221 TGGACAAACG CAACAGCCCT AAGCAGGGGG AGTCCGACAA ACCGCAGGGG ACCGCGGAAA 
     2281 AGTCAGCTGC TCACAGCAAT CTCGGCCACA GCAGGATAGG ATCAAGCTCA AATAGTGATG 
     2341 GGCACGATTC CTATGAATTC GACGACGAGT CTATGCAGGG GGACGATCCT AAATCATCCG 
     2401 ATGAAAGTAA CGGCAGCGAC GAATCCGACA CTAACAGTGA ATCAGCCAAC GAGAGCGGAT 
     2461 CTAGAGGGGA CGCTTCATAC ACTTCCGATG AATCATCTGA CGACGATAAC GATTCTGATA 
     2521 GTCATGCTGG CGAAGATGAT TCATCTGATG ATTCCAGCGA CACTGACGAT AGCGACTCCA 
     2581 ACGGGGACGG GGACAGCGAT AGTAACGGGG ACGGAGACAG CGAGAGCGAA GATAAGGATG 
     2641 AGTCCGATTC ATCCGACCAT GACAACTCCA GTGATAGTGA GTCCAAGTCC GACTCTTCCG 
     2701 ATTCCAGCGA CGATAGCTCA GATTCTTCCG ACTCCTCTGA TAGTAGCGAT TCTTCTGACT 
     2761 CCTCAGATAG CTCTGACAGC AGCGACTCCA GTGACAGCTC CGATTCTAAC AGCTCCAGCG 
     2821 ACTCCTCCGA CAGCAGTTCT TCAAGTGATT CATCTGATTC CTCAGATAGC AGTGACAGCT 
     2881 CTGATAGCTC AGACAGCTCT GATAGCAGCG ACTCTAGCGA TTCCAGCGAT TCATCTGACA 
     2941 GTAGTGACTC CAGCGACTCA TCCGACAGTA GTGACTCCTC CAGCTCATCA GACTCATCTG 
     3001 ATTCCAGTAG TTCTTCAGAT TCCTCCGACA GTAGCGATAG CTCCGATTCT AGTGACTCCT 
     3061 CCGACTCCAG CGACTCCAGT TCAAGTGACA GCAGTAGCAG CTCCAATAGT AGCGACTCCA 
     3121 GTGACTCCAG TGACTCCTCA TCAAGCAGCG ACTCTTCCAA TTCATCCGAC TCTTCCGACA 
     3181 GTTCAGATAG TAGCGATTCC TCCGACTCCT CAGACAGCTC CAATTCTAGC GATAGTTCCG 
     3241 ATTCCTCCAG CAGCTCTGAC TCCTCAGATT CCTCTTCCTC CTCCGATTCT TCCGATAGTA 
     3301 GTGATAGTTC TGACAGCTCC GAGTCCTCTG AGAGTAGTGA TTCTTCTAAT AGCAGCGACT 
     3361 CCAGTGACTC TTCCGATTCT TCTGATTCAA GCGATTCTAG CGACAGTAGC GACTCTAGCG 
     3421 ACTCCAGCGA CAGTTCAAAC AGTTCCGACA GTAGCGATAG CAGCGACTCT TCCGATAGCA 
     3481 GTGACTCTAG TAACTCTTCA GACTCAAGTG ATAGTTCTGA CTCAAGCGAT TCAAGCGACA 
     3541 GTTCAGATAG CTCTGATAGT TCTGACTCCA GTGATAGTAG CGACTCATCC GACAGCTCTG 
     3601 ATTCATCTGA TAGCTCCGAC TCCTCCGATA GTTCAGACAG TAGCGATAGT AGCGACTCAA 
     3661 GCGACAGCAG CGATTCCTCC GACTCCTCAG ATAGTAGCAA CAGTTCCGAC TCTTCTGACA 
     3721 GCGATTCCAA GGATTCTTCT AGTGATTCTT CTGACGGGGA CAGTAAAAGT GGCAATGGCA 
     3781 ACAGCGATTC CAATAGTGAC TCAAATTCCG ACTCTGACTC CGACAGCGAG GGGAGCGACT 
     3841 CTAATCACAG CACGTCCGAC GACACGCGTA CGCGGCCGCT CGAGATGGAG AGCGACGAGA 
     3901 GCGGCCTGCC CGCCATGGAG ATCGAGTGCC GCATCACCGG CACCCTGAAC GGCGTGGAGT 
     3961 TCGAGCTGGT GGGCGGCGGA GAGGGCACCC CCGAGCAGGG CCGCATGACC AACAAGATGA 
     4021 AGAGCACCAA AGGCGCCCTG ACCTTCAGCC CCTACCTGCT GAGCCACGTG ATGGGCTACG 
     4081 GCTTCTACCA CTTCGGCACC TACCCCAGCG GCTACGAGAA CCCCTTCCTG CACGCCATCA 
     4141 ACAACGGCGG CTACACCAAC ACCCGCATCG AGAAGTACGA GGACGGCGGC GTGCTGCACG 
     4201 TGAGCTTCAG CTACCGCTAC GAGGCCGGCC GCGTGATCGG CGACTTCAAG GTGATGGGCA 
     4261 CCGGCTTCCC CGAGGACAGC GTGATCTTCA CCGACAAGAT CATCCGCAGC AACGCCACCG 
     4321 TGGAGCACCT GCACCCCATG GGCGATAACG ATCTGGATGG CAGCTTCACC CGCACCTTCA 
     4381 GCCTGCGCGA CGGCGGCTAC TACAGCTCCG TGGTGGACAG CCACATGCAC TTCAAGAGCG 
     4441 CCATCCACCC CAGCATCCTG CAGAACGGGG GCCCCATGTT CGCCTTCCGC CGCGTGGAGG 
     4501 AGGATCACAG CAACACCGAG CTGGGCATCG TGGAGTACCA GCACGCCTTC AAGACCCCGG 
     4561 ATGCAGATGC CGGTGAAGAA AGAGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4621 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4681 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4741 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4801 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4861 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4921 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4981 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5041 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5101 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5161 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5221 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCTTG GCTGTGGAAT 
     5281 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5341 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     5401 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC 
     5461 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 
     5521 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA 
     5581 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC 
     5641 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC 
     5701 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA 
     5761 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT 
     5821 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG 
     5881 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA 
     5941 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 
     6001 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG 
     6061 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG 
     6121 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC 
     6181 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT 
     6241 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 
     6301 TGTGGCCGGC TGGGTGTGGC CGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT 
     6361 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT 
     6421 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC 
     6481 TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA 
     6541 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA 
     6601 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG 
     6661 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT 
     6721 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC 
     6781 CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT 
     6841 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG 
     6901 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT 
     6961 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT 
     7021 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 
     7081 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 
     7141 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 
     7201 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 
     7261 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 
     7321 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 
     7381 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG 
     7441 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 
     7501 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 
     7561 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 
     7621 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC 
     7681 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 
     7741 CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 
     7801 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 
     7861 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 
     7921 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 
     7981 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 
     8041 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 
     8101 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 
     8161 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 
     8221 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 
     8281 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 
     8341 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 
     8401 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 
     8461 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 
     8521 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 
     8581 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 
     8641 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 
     8701 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 
     8761 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 
     8821 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 
     8881 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 
     8941 CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 
     9001 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 
     9061 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 
     9121 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 
     9181 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 
     9241 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 
     9301 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 
     9361 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//