LOCUS       MG224432                8210 bp    DNA     circular  SYN 
DEFINITION  Lgi2 (tGFP-tagged) - Mouse leucine-rich repeat LGI family member 2 (Lgi2), (10ug)
ACCESSION   NM_144945
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2691..3395 
                     /label="GFP"  
     misc_feature    3442..4027 
                     /label="PolyA signal"  
     misc_feature    6119..6764 
                     /label="ColE1"  
     misc_feature    4481..5275 
                     /label="Neo"  
     misc_feature    4112..4414 
                     /label="SV40 ori"  
     misc_feature    6779..7639 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2670..2676 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2670 
                     /label="Lgi2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCTATGG AGAGGCGGCG GGGCGCTGGG GTTGCTGCTG CTGAGCGCCG 
     1081 CGTGCCTCAT CCCGCCGAGC GCGCAGGTGA GGCGGTTGGC GCGCTGCCCC GCCACTTGCA 
     1141 GCTGTACCAA GGAGTCCATC ATCTGCGTGG GCTCCTCTTG GGTGCCTAGG ATCGTGCCTG 
     1201 GCGACATCAG CTCCCTGAGC CTAGTAAATG GAACATTTCT GGAAATCAAG GACCGAATGT 
     1261 TTTCCCACCT GCCCTCTCTG CAGCTGCTAT TGCTGAATTC TAACTCATTC ACGGTGATCC 
     1321 GGGACGATGC CTTTGCTGGC CTTTTTCATC TTGAATACCT GTTCATTGAA GGGAACAAAA 
     1381 TAGAAACCAT TTCAAGAAAT GCCTTTCGGG GCCTCCGTGA CCTGACTCAC CTAGACTTAA 
     1441 GGGGTAATAA ATTTGAATGT GACTGCAAAG CCAAATGGCT GTATCTATGG CTGAAGATGA 
     1501 CAAACTCCAC CGTCTCTGAT GTGCTGTGTA TCGGTCCACC AGAATACCAG GAGAAGAAAC 
     1561 TCAACGAAGT GACCAGCTTT GACTATGAGT GTACCACCAC AGGTCCCCAG ACTGATGAAG 
     1621 CCAAGCAGAG AGGATGGCAG TTGGAACTCT CCCTGGGATT TTGTGAACTA ATATTTGTTT 
     1681 TTCAACACCC ACTCTCAGAT TTTGTCGTTC ATCAGACTCT GCCGTACCAG TCGGTGTCAG 
     1741 TAGACACGTT CAACTCCAAG AACGATGTGT ACGTGGCCAT CGCTCAGCCC AGCATGGAGA 
     1801 ACTGCATGGT GTTGGAGTGG GACCACATAG AAATGAATTT CCGGAGTTAT GACAATATCA 
     1861 CAGGCCAGTC CATCGTGGGC TGCAAGGCCA TCCTCATTGA CGACCAGGTC TTTGTGGTGG 
     1921 TGGCCCAGCT CTTCGGTGGC TCTCACATTT ACAAATACGA CGAGAGCTGG ACCAAGTTTG 
     1981 TCAAGTTCCA AGACATAGAG GTGTCTCGGA TTTCCAAGCC CAACGACATT GAGCTGTTCG 
     2041 AGATCGACGA CGAGACCTTC TTCATCATCG CCGACAGCTC CAAAGCGGGG CTGTCCACAG 
     2101 TTTACAAGTG GAACAGCAAA GGGTTTTACT CCTACCAGTC ACTACATGAG TGGTTCAGGG 
     2161 ACACAGATGC GGAGTTTGTT GACATCGACG GGAAGTCTCA CCTCATCCTG TCTAGCCGCT 
     2221 CCCAGGTCCC CATCATCCTG CAGTGGAATA AAAGTTCCAA GAAGTTTGTC CCCCATGGTG 
     2281 ACATCCCCAA TATGGAGGAT GTGCTGGCTG TGAAGAGCTT CCGGATGCAG AACACCCTCT 
     2341 ACCTTTCCCT CACCCGTTTC ATTGGGGACT CGCGGGTCAT GCGTTGGAAC AGTAAGCAGT 
     2401 TTGTGGAGGT CCAGGCTCTT CCTTCCCGGG GGGCTATGAC CTTGCAACCC TTCTCCTTCA 
     2461 AAGATAACCA CTACCTGGCC CTGGGGAGTG ACTACACGTT CTCACAGATC TACCAGTGGG 
     2521 ATAAAGAGAA ACAGCAGTTT AAAAAGTTTA AAGAAATCTA TGTGCAGGCG CCTCGGTCCT 
     2581 TCACAGCCGT CTCCACCGAC AGGAGAGATT TCTTTTTTGC ATCCAGTTTC AAAGGGAAGA 
     2641 CAAAGATTTT TGAACATATC ATTGTTGATT TAAGTTTGAC GCGTACGCGG CCGCTCGAGA 
     2701 TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA TGGAGATCGA GTGCCGCATC ACCGGCACCC 
     2761 TGAACGGCGT GGAGTTCGAG CTGGTGGGCG GCGGAGAGGG CACCCCCGAG CAGGGCCGCA 
     2821 TGACCAACAA GATGAAGAGC ACCAAAGGCG CCCTGACCTT CAGCCCCTAC CTGCTGAGCC 
     2881 ACGTGATGGG CTACGGCTTC TACCACTTCG GCACCTACCC CAGCGGCTAC GAGAACCCCT 
     2941 TCCTGCACGC CATCAACAAC GGCGGCTACA CCAACACCCG CATCGAGAAG TACGAGGACG 
     3001 GCGGCGTGCT GCACGTGAGC TTCAGCTACC GCTACGAGGC CGGCCGCGTG ATCGGCGACT 
     3061 TCAAGGTGAT GGGCACCGGC TTCCCCGAGG ACAGCGTGAT CTTCACCGAC AAGATCATCC 
     3121 GCAGCAACGC CACCGTGGAG CACCTGCACC CCATGGGCGA TAACGATCTG GATGGCAGCT 
     3181 TCACCCGCAC CTTCAGCCTG CGCGACGGCG GCTACTACAG CTCCGTGGTG GACAGCCACA 
     3241 TGCACTTCAA GAGCGCCATC CACCCCAGCA TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT 
     3301 TCCGCCGCGT GGAGGAGGAT CACAGCAACA CCGAGCTGGG CATCGTGGAG TACCAGCACG 
     3361 CCTTCAAGAC CCCGGATGCA GATGCCGGTG AAGAAAGAGT TTAAACGGCC GGCCGCGGTC 
     3421 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3481 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3541 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3601 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3661 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     3721 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     3781 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     3841 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     3901 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     3961 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4021 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4081 GCTTGGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     4141 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC 
     4201 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG 
     4261 CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT 
     4321 GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC 
     4381 CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT 
     4441 TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA 
     4501 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC 
     4561 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG 
     4621 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG 
     4681 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT 
     4741 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG 
     4801 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG 
     4861 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA 
     4921 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG 
     4981 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT 
     5041 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT 
     5101 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCCGACC GCTATCAGGA CATAGCGTTG 
     5161 GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT 
     5221 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC 
     5281 TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC 
     5341 GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG 
     5401 ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA 
     5461 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA 
     5521 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT 
     5581 ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT 
     5641 TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA 
     5701 AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC 
     5761 TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG 
     5821 CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC 
     5881 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 
     5941 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 
     6001 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 
     6061 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 
     6121 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 
     6181 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 
     6241 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 
     6301 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 
     6361 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 
     6421 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 
     6481 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 
     6541 CCGGCAAACA AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 
     6601 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 
     6661 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 
     6721 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 
     6781 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 
     6841 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 
     6901 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 
     6961 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 
     7021 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 
     7081 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 
     7141 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 
     7201 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 
     7261 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 
     7321 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 
     7381 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 
     7441 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 
     7501 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 
     7561 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 
     7621 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 
     7681 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 
     7741 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT 
     7801 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG 
     7861 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC 
     7921 AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC 
     7981 CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT 
     8041 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA 
     8101 CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG 
     8161 CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//