LOCUS       MG224145                9254 bp    DNA     circular  SYN 
DEFINITION  Taok3 (tGFP-tagged) - Mouse TAO kinase 3 (Taok3), (10ug)
ACCESSION   NM_001081308
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3735..4439 
                     /label="GFP"  
     misc_feature    4486..5071 
                     /label="PolyA signal"  
     misc_feature    7163..7808 
                     /label="ColE1"  
     misc_feature    5525..6319 
                     /label="Neo"  
     misc_feature    5156..5458 
                     /label="SV40 ori"  
     misc_feature    7823..8683 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3714..3720 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3714 
                     /label="Taok3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGTAAAGGG GCACTGAAGG ACCCAGAGAT TGCTGATCTG TTCTTCAAGG 
     1081 ATGACCCCGA AGAGCTTTTT ATCGATTTGC ATGAAATTGG ACATGGGAGC TTTGGAGCCG 
     1141 TGTACTTTGC GACAAATGCG CACACCAACG AGGTGGTCGC TGTTAAGAAA ATGTCCTACA 
     1201 GTGGGAAGCA GACCCATGAG AAATGGCAAG ATATCCTTAA GGAAGTTAAG TTTCTGCAGC 
     1261 AGCTGAAGCA TCCTAACACC ATCGAGTACA AAGGCTGTTA TTTAAAGGAG CACACTGCAT 
     1321 GGTTGGTGAT GGAGTACTGC CTAGGTTCAG CCTCTGACTT GTTAGAAGTT CATAAGAAAC 
     1381 CACTTCAGGA AGTGGAGATC GCTGCTATCA CCCATGGTGC CTTGCAGGGG CTGGCCTACC 
     1441 TCCACTTTCA CTCCCTGATT CACAGGGACA TTAAAGCAGG GAACATCCTC CTCACAGAGC 
     1501 CAGGTCAGGT GAAGCTGGCT GACTTCGGAT CTGCCTCCAT GGCTTCTCCC GCCAACTCCT 
     1561 TTGTGGGGAC TCCTTACTGG ATGGCCCCAG AGGTAATCTT AGCTATGGAC GAAGGGCAGT 
     1621 ACGACGGGAA AGTTGACATC TGGTCGCTTG GCATCACCTG CATAGAGTTG GCGGAGCGGA 
     1681 AGCCCCCCCT CTTCAACATG AACGCCATGA GCGCCCTCTA CCACATCGCT CAGAATGACT 
     1741 CCCCCACGCT ACAGTCCAGG GAATGGACAG ACTCCTTCAG GAGATTCGTT GATTACTGCT 
     1801 TGCACAAAAT ACCTCAGGAA AGACCAGCCG CGGTGGAGCT GTTGCGGCAT GACTTCATTC 
     1861 GGAGGGAGCG GCCACCGAAG GTCCTCATTG ATCTCATACA GAGGACAAAA GATGCTGTCC 
     1921 GAGAGCTGGA CAACCTGCAG TACAGGAAAA TGAAGAAGAT CCTCTTCCAG GAGACTCGGA 
     1981 ACGGACCCTT GAATGAGTCG CAAGAAGAAG AAGAAGATGG TGAGCAAGGA AGCAACCTGA 
     2041 ACCGAGAGGT GGACAGTCTG GGCAGCATCC ACTCCATTCC CAGCACATCA GTGAGCACAG 
     2101 GCAGCCGGAG CAGCAGTGTG AACAGCATGC AGGAGGTCAT GGATGAGAGC AGCTCTGAGC 
     2161 TCGTCATGAT GCAGGAGGAT GAAGGCACAG CCAACTCCAG CGCCTCCACG GTACACAAGA 
     2221 AGGATCATGT ATTCGTAAGG GATGAGGCGG GCCACGGCGA TCCCAGGCCT GAGCCGCGGC 
     2281 CTACCCAGTC AGTTCAGAGC CGGGCCCTCC ACTATCGGAA CCGAGAGCGC TTTGCCACGA 
     2341 TCAAATCCGC GTCTTTGGTT ACACGGCAGA TCCATGAGCA TGAGCAGGAG AACGAGCTGC 
     2401 GGGAACAGAT GTCAGGTTAT AAGCGGATGC GGCGCCAGCA CCAGAAGCAG CTGATCGCCC 
     2461 TGGAGAACAA GCTGAAGGCT GAGATGGATG AGCACCGCCT CAAGCTCCAG AAGGAGGTGG 
     2521 AGACGCACGC CAACAACTCG TCCATCGAGC TGGAGAAGCT GGCCAAGAAG CAAGTGGCTA 
     2581 CCATAGAAAA GGAGGCAAAG GTAGCCGCAG CGGATGAGAA GAAGTTCCAG CAGCAGATCC 
     2641 TGGCCCAGCA GAAGAAGGAC TTGACAACTT TCTTAGAAAG TCAGAAGAAG CAGTACAAGA 
     2701 TTTGTAAGGA GAAAATAAAA GAGGAAATGA ACGAGGACCA TAGCACACCC AAGAAGGAGA 
     2761 AGCAGGAGCG GATATCGAAG CATAAAGAAA ACCTGCAGCA CACGCAGGCC GAGGAGGAAG 
     2821 CCCACCTGCT CACTCAACAG AGGCTGTACT ACGACAGAAA CTGCCGCTTC TTCAAACGGA 
     2881 AAATAATGAT CAAGCGCCAC GAGGTGGAAC AGCAGAACAT TCGGGAGGAA TTAAATAAGA 
     2941 AGAGGACCCA GAAGGAAATG GAGCACGCCA TGCTGATCCG GCATGATGAA TCCACCCGAG 
     3001 AGCTGGAGTA CAGGCAGCTG CACACCCTGC AGAAGCTCCG CATGGATCTG ATCCGGCTGC 
     3061 AGCACCAGAC GGAACTGGAG AACCAGCTGG AGTACAATAA GAGGCGGGAG CGGGAGCTGC 
     3121 ACCGGAAGCA CGTCATGGAG CTTCGGCAAC AGCCGAAAAA CTTAAAGGCC ATGGAAATGC 
     3181 AAATTAAAAA ACAGTTTCAG GACACTTGCA AAGTACAGAC CAAACAGTAC AAAGCCCTCA 
     3241 AAAATCACCA GTTGGAAGTC ACTCCAAAGA ATGAGCACAA AGCAATCCTA AAGACACTGA 
     3301 AGGACGAGCA GACGAGGAAG CTGGCCATTT TGGCCGAGCA GTATGAACAG AGTATAAACG 
     3361 AGATGATGGC CTCTCAAGCG TTACGGCTAG ACGAGGCACA AGAGGCAGAG TGCCAGGCCT 
     3421 TGAGACTACA GCTCCAGCAG GAGATGGAGC TGCTCAACGC CTACCAGAGC AAAATCAAGA 
     3481 TGCAAACGGA GGCCCAGCAC GAGCGCGAGC TACAGAAGCT AGAGCAGAGG GTGTCCCTGC 
     3541 GCAGAGCGCA TCTTGAGCAG AAGATTGAAG AGGAGCTGGC TGCCCTGCAG AAGGAACGCA 
     3601 GTGAGAGGAT CAAGACTCTC CTGGAGAGAC AAGAGCGAGA GACCGAGACT TTTGACATGG 
     3661 AGAGCCTCAG AATGGGCTTT GGGAATTTGG TGACATTAGA TTTTCCTAAG GAGGACTATA 
     3721 GAACGCGTAC GCGGCCGCTC GAGATGGAGA GCGACGAGAG CGGCCTGCCC GCCATGGAGA 
     3781 TCGAGTGCCG CATCACCGGC ACCCTGAACG GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG 
     3841 AGGGCACCCC CGAGCAGGGC CGCATGACCA ACAAGATGAA GAGCACCAAA GGCGCCCTGA 
     3901 CCTTCAGCCC CTACCTGCTG AGCCACGTGA TGGGCTACGG CTTCTACCAC TTCGGCACCT 
     3961 ACCCCAGCGG CTACGAGAAC CCCTTCCTGC ACGCCATCAA CAACGGCGGC TACACCAACA 
     4021 CCCGCATCGA GAAGTACGAG GACGGCGGCG TGCTGCACGT GAGCTTCAGC TACCGCTACG 
     4081 AGGCCGGCCG CGTGATCGGC GACTTCAAGG TGATGGGCAC CGGCTTCCCC GAGGACAGCG 
     4141 TGATCTTCAC CGACAAGATC ATCCGCAGCA ACGCCACCGT GGAGCACCTG CACCCCATGG 
     4201 GCGATAACGA TCTGGATGGC AGCTTCACCC GCACCTTCAG CCTGCGCGAC GGCGGCTACT 
     4261 ACAGCTCCGT GGTGGACAGC CACATGCACT TCAAGAGCGC CATCCACCCC AGCATCCTGC 
     4321 AGAACGGGGG CCCCATGTTC GCCTTCCGCC GCGTGGAGGA GGATCACAGC AACACCGAGC 
     4381 TGGGCATCGT GGAGTACCAG CACGCCTTCA AGACCCCGGA TGCAGATGCC GGTGAAGAAA 
     4441 GAGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4501 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4561 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4621 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4681 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4741 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4801 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4861 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4921 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4981 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5041 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5101 CCTGTTGAAT TGGGTACGCG GCCAGCTTGG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA 
     5161 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5221 ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     5281 AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC 
     5341 AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG 
     5401 GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC 
     5461 TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA 
     5521 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG 
     5581 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC 
     5641 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT 
     5701 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT 
     5761 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC 
     5821 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC 
     5881 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC 
     5941 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG 
     6001 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG 
     6061 GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT 
     6121 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCC 
     6181 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA 
     6241 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC 
     6301 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC 
     6361 AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT 
     6421 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA 
     6481 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA 
     6541 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT 
     6601 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT 
     6661 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC 
     6721 ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC 
     6781 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC 
     6841 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG 
     6901 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 
     6961 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 
     7021 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 
     7081 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 
     7141 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 
     7201 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 
     7261 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 
     7321 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 
     7381 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 
     7441 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 
     7501 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 
     7561 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG 
     7621 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 
     7681 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 
     7741 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 
     7801 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA 
     7861 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA 
     7921 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC 
     7981 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA 
     8041 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG 
     8101 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG 
     8161 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG 
     8221 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG 
     8281 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC 
     8341 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT 
     8401 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA 
     8461 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA 
     8521 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA 
     8581 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC 
     8641 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC 
     8701 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 
     8761 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 
     8821 CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA 
     8881 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG 
     8941 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT 
     9001 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG 
     9061 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA 
     9121 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT 
     9181 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT 
     9241 TTAACGCGAA TTTT
//