LOCUS       MG220716                9539 bp    DNA     circular  SYN 
DEFINITION  Dsg3 (tGFP-tagged) - Mouse desmoglein 3 (Dsg3), (10ug)
ACCESSION   NM_030596
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4020..4724 
                     /label="GFP"  
     misc_feature    4771..5356 
                     /label="PolyA signal"  
     misc_feature    7448..8093 
                     /label="ColE1"  
     misc_feature    5810..6604 
                     /label="Neo"  
     misc_feature    5441..5743 
                     /label="SV40 ori"  
     misc_feature    8108..8968 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3999..4005 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3999 
                     /label="Dsg3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCTGCCTC TTCCCTAGAG CCTTGGGGTC TCTGGCCCTC CTGATGGTGG 
     1081 TGCTGCTGGT ACAGGGGGAA CTGCATGTGA AGCCGGGAGG ACAGCACAGA GAAGATGGGA 
     1141 CAGCATTGCA GCTGGCCAAA AGAAGGTACA AACGTGAATG GGTGAAATTT GCAAAACCCT 
     1201 GCAGAGAAAG AGAAGACAAC TCAAGACGAA ACCCAATTGC CAAGATCACT TCAGATTTCC 
     1261 AAAAAAACCA GAAAATTACC TACCGCATTT CTGGAGTGGG AATTGATCAG CCTCCCTTTG 
     1321 GGATCTTTGT TGTTGACCCA AACAATGGTG ATATCAACAT AACAGCCATA GTTGATCGTG 
     1381 AGGAAACCCC AAGCTTCCTG ATCACATGCC GTGCACTAAA TGCCCTGGGA CAGGATGTAG 
     1441 AGAGACCACT TATATTAACA GTGAAAATTT TAGACGTCAA TGATAACCCT CCAATATTTT 
     1501 CACAAACCAT ATTCAAGGGT GAAATTGAAG AAAATAGTGC TTCAAATTCC CTGGTAATGA 
     1561 TCCTAAATGC CACGGATGCA GATGAACCAA ACCATATGAA CTCTAAAATT GCCTTCAAAA 
     1621 TTGTCTCCCA GGAGCCCGCA GGCATGTCCA TGTTCCTCAT CAGCAGAAAC ACCGGAGAAG 
     1681 TCCGCACTTT GACCAGTTCT CTTGATCGAG AGCAAATTAG TAGCTACCAT CTGGTTGTGA 
     1741 GCGGTGCAGA CAACGATGGA ACAGGACTGT CAACTCAGTG TGAATGTAGT ATTAAAATTA 
     1801 AAGATGTCAA TGACAACTTC CCAGTGCTCA GAGAGTCTCA GTATTCAGCA CGTATTGAAG 
     1861 AAAATACTTT GAATGCAGAG CTACTCCGAT TTCAAGTGAC AGACTGGGAT GAAGAATACA 
     1921 CAGATAATTG GTTGGCTGTG TATTTCTTCA CCTCTGGGAA TGAGGGGAAT TGGTTTGAAA 
     1981 TTGAAACAGA TCCTAGAACC AATGAAGGCA TCCTGAAGGT GGTTAAGGCT CTGGATTATG 
     2041 AACAAGTGCA GAGTATGCAG TTTAGTATTG CTGTCAGAAA CAAAGCTGAG TTCCACCAGT 
     2101 CAGTGATCTC TCAGTACCGA GTGCAGTCAA CGCCAGTCAC AATTCAAGTC ATTGATGTCC 
     2161 GAGAGGGAAT CTCATTCCGT CCTCCTTCCA AGACATTTAC TGTGCAAAGG GGTGTAAGCA 
     2221 CTAACAAATT AGTTGGTTAC ATCCTGGGGA CATACCAAGC TACTGATGAG GACACGGGTA 
     2281 AAGCTGCCTC CTCTGTCAGA TATGTCCTGG GTCGTAATGA TGGTGGTTTG CTTGTTATTG 
     2341 ATTCAAAGAC TGCTCAGATC AAATTTGTCA AAAACATCGA CCGAGATTCT ACTTTCATAG 
     2401 TTAACAAGAC AATTTCTGCT GAGGTTCTGG CCATAGACGA AAACACTGGT AAGACCTCCA 
     2461 CAGGCACAAT ATATGTGGAA GTGCCCAGTT TTAATGAAAA CTGCCCGTCA GTTGTCCTCG 
     2521 AGAAGAAAGA CATCTGTACT TCCTCACCTT CTGTGACCCT CTCAGTGAGA ACCCTGGACA 
     2581 GGGGTAAATA CACTGGCCCC TACACAGTGT CCCTGGAGGA GCAGCCACTG AAGCTGCCAG 
     2641 TCATGTGGAC CATCACGACG CTGAATGCTA CATCAGCTCT TCTACAAGCC CAGCAGCAAG 
     2701 TGTCTCCAGG AGTGTACAAT GTCCCAGTCA TCGTGAAGGA CAATCAGGAT GGGCTGTGTG 
     2761 ACACACCAGA GAGCTTGACC CTGACAGTGT GTCAATGTGA TGACCGGAGC ATGTGCAGAG 
     2821 CCCCTATCCC GAGCAGAGAG CCTAACACAT ATGGGGAGTC ATCCTGGAGG CTGGGGCCTG 
     2881 CTGCCATTGG CTTGATCCTG CTAGGACTCC TGATGTTGCT ATTGGCCCCA CTTCTGCTTC 
     2941 TGACCTGTGA CTGTGGGTCA GGTCCCATTG GAGGAGCAGC GACAGGTGGC TTTATCCCAG 
     3001 TGCCCGATGG TTCGGAAGGT ACAATTCATC AGTGGGGAAT CGAAGGAGCT CAGCCCGAAG 
     3061 ACAAGGAAAT CACAAATATT TGTGTTCCTC CAGTTACAAC CAATGGAGCC GATTTTATGG 
     3121 AAAGCTCTGA AGTTTGCACA AATACATATG CTGGTGGGAC CATGGTAGAA GGTGCTTCAG 
     3181 GAATGGAAAT GATTACCAAG CTGGGAGGAG CTACTGGAGC TACAGCGGCA CTTGGTCCCT 
     3241 GTTCCTTGGG TTATTCAGGA ACAATGAGAA CAAGGCATTC CACTGGAGGG ACCTTGAAGG 
     3301 ACTATGCTGC GCCAGTCAAC ATGACTTTCC TAGGCTCCTA TTTCTCTCAG AAATCACTCG 
     3361 CCTATGCCGA GGAAGAGGAT GAGCGCGAAG TGAACGACTG TTTGCTGATC TACGATGATG 
     3421 AAGGCGAGGA TGCTGCCCCC CACTCCCCCA CCCTCAGCTC CTGCAGCATT TTTGCCGATG 
     3481 ACCTAGACGA CAACTTCTTG GATTCCCTTG GCCCCAAATT TAAAAAGCTT GCAGAGATCT 
     3541 GTCTCGGTAT TGATGACGAA GCCAAGCAAG CAAAGCCAGG CCCTAAAGAC AGTGGTTCTG 
     3601 GGGCTGATAC CTGCGCTCGC TCTATGGAAG TCCCTCAGTC AGGTTCTAAC AGGTACCAGA 
     3661 CTTTGCCTGG CAGTCTGGAA GTCACCCAAA CGGGTTCTAA AATATGCCAC ACGCTGTCAG 
     3721 GCAACCAAGA AACTTCTGTT ATGTCCACTT CTGGGTCTGT CCACCCTGCT GTTGCCATCC 
     3781 CTGACCCTCT ACAGCTTGGG AACTACTTGC TCACAGAAAC TTACTCAACT TCAGGTTCCT 
     3841 TTGCACAACC CACCACTGTT ACCTTTGACC CCCATGTCAC ACAAAATGTC ACTGTAACAG 
     3901 AAAGGGTCAT TTGTCCCCTT CCTAGTGCCT CCAGCAGTAT AGTGGCTCCC ACAGAACTGC 
     3961 GAGGCTCATA CAATATGCTC TATACAAAAG AAACCTGTTC CCATCTAACG CGTACGCGGC 
     4021 CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC TGCCCGCCAT GGAGATCGAG TGCCGCATCA 
     4081 CCGGCACCCT GAACGGCGTG GAGTTCGAGC TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC 
     4141 AGGGCCGCAT GACCAACAAG ATGAAGAGCA CCAAAGGCGC CCTGACCTTC AGCCCCTACC 
     4201 TGCTGAGCCA CGTGATGGGC TACGGCTTCT ACCACTTCGG CACCTACCCC AGCGGCTACG 
     4261 AGAACCCCTT CCTGCACGCC ATCAACAACG GCGGCTACAC CAACACCCGC ATCGAGAAGT 
     4321 ACGAGGACGG CGGCGTGCTG CACGTGAGCT TCAGCTACCG CTACGAGGCC GGCCGCGTGA 
     4381 TCGGCGACTT CAAGGTGATG GGCACCGGCT TCCCCGAGGA CAGCGTGATC TTCACCGACA 
     4441 AGATCATCCG CAGCAACGCC ACCGTGGAGC ACCTGCACCC CATGGGCGAT AACGATCTGG 
     4501 ATGGCAGCTT CACCCGCACC TTCAGCCTGC GCGACGGCGG CTACTACAGC TCCGTGGTGG 
     4561 ACAGCCACAT GCACTTCAAG AGCGCCATCC ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA 
     4621 TGTTCGCCTT CCGCCGCGTG GAGGAGGATC ACAGCAACAC CGAGCTGGGC ATCGTGGAGT 
     4681 ACCAGCACGC CTTCAAGACC CCGGATGCAG ATGCCGGTGA AGAAAGAGTT TAAACGGCCG 
     4741 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4801 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4861 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4921 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4981 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5041 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5101 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5161 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5221 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5281 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5341 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5401 ACGCGGCCAG CTTGGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC 
     5461 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG 
     5521 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 
     5581 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT 
     5641 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT 
     5701 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC 
     5761 CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC 
     5821 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC 
     5881 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA 
     5941 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG 
     6001 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG 
     6061 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG 
     6121 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG 
     6181 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC 
     6241 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA 
     6301 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC 
     6361 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT 
     6421 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCCGACCG CTATCAGGAC 
     6481 ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC 
     6541 CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT 
     6601 GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC 
     6661 TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG 
     6721 TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG 
     6781 CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA 
     6841 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 
     6901 ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT 
     6961 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG 
     7021 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT 
     7081 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG 
     7141 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 
     7201 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 
     7261 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 
     7321 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 
     7381 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 
     7441 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 
     7501 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 
     7561 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 
     7621 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 
     7681 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 
     7741 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 
     7801 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 
     7861 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA 
     7921 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 
     7981 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 
     8041 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 
     8101 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 
     8161 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 
     8221 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 
     8281 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 
     8341 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 
     8401 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 
     8461 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 
     8521 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 
     8581 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 
     8641 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 
     8701 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 
     8761 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 
     8821 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 
     8881 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 
     8941 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 
     9001 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 
     9061 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA 
     9121 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA 
     9181 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 
     9241 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 
     9301 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 
     9361 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 
     9421 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC 
     9481 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//