LOCUS       MG220390                9083 bp    DNA     circular  SYN 
DEFINITION  Fstl4 (tGFP-tagged) - Mouse follistatin-like 4 (Fstl4), (10ug)
ACCESSION   NM_177059
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3564..4268 
                     /label="GFP"  
     misc_feature    4315..4900 
                     /label="PolyA signal"  
     misc_feature    6992..7637 
                     /label="ColE1"  
     misc_feature    5354..6148 
                     /label="Neo"  
     misc_feature    4985..5287 
                     /label="SV40 ori"  
     misc_feature    7652..8512 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3543..3549 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3543 
                     /label="Fstl4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAACCAGGA GGCTTCTGGC CACATCTTGC CCTACTCGGG GTCTCCCTGC 
     1081 CGGCCGTGCT GGGATGGATG GACCAAGGAG CCAGCAGAAG CCCGAATATG GTTCCAGGAG 
     1141 AGTCGCAGGC AGAGGAAACC AGAGGCTTTG AAGTCACAAG AAGAGAAGGT CTTCCCAGCC 
     1201 CTGGGCTGTC AGCCTCCTGT GGGAAGAAAC TCTGCAGCCA TGGGAGCAGG TGCTTGCTCA 
     1261 ATAGGACGAC AGGGCAGCCC TCGTGCCAGT GTCTGGAGGT GTGCAGGCCC CGCTACATGC 
     1321 CTGTGTGTGG CTCCGACGGG AGGCTCTACG GGAACCACTG TGAGCTTCGC CGTGCAGCCT 
     1381 GCCTGCTGGG CAAGCGAATT GTCAGTGTAC ACAGCAAGGA CTGCTTTCTC AAAGGAGACA 
     1441 TGTGCACCAT GGCTGGCTAT GCTCGTCTCA AAAATGTCCT CCTGGCACTC CAGAGCCGCA 
     1501 GACAGCCGCT CCCACAAGGA ACCCCCAGGC AAAGCCTCGC CTCCCAGAAG CGCCTCTTGG 
     1561 TGGAGTCACT GTTTAAGGAC TTGGATGCCG ACGGCAATGG GCACCTCGGC AGCTTGGAGC 
     1621 TGGCTCAGTA TGTGCTAAAG GAGCAGGACA TGGACGGTTC ACTGAACAGA TGCTCACCCA 
     1681 GCGACCTCCT GCGATTTGAT GACTATAATA GCGATGGCTC CTTGACCCTT GGAGAGTTCT 
     1741 ACACAGCCTT CCAGGTGATT CAGCTAAGCC TCGCCCCTGA GGACAAGGTC AGTGTGACTA 
     1801 CCGTGACCGT GGGCCTGAGC ACAGTGCTGA CCTGTGCTAT TCGTGGAGAC CTGAGGCCAC 
     1861 CAATCATCTG GAAACGCAAC GGGCTCACTC TGAGCTTCCT AGGCCTGGAA GACATCAATG 
     1921 ACTTTGGCGA GGATGGTTCC CTGTACATCA CCAAGGTGAC CACAGTCCAC ATGGGCAACT 
     1981 ACACCTGCCA CGCCTTGGGC CATGAGCAGC TGGTCCAGAC CCACGTCCTG CAGGTGAATG 
     2041 TGCCACCAGT CATTCGTGTC TATCCAGAAA CCCAGGCACA AGAACCTGGG GTGGCAGCCA 
     2101 GCCTTCGATG CCATGCTGAG GGCATCCCCC TGCCCAGAAT CATTTGGCTG AAAAATGGCA 
     2161 TGGATGTCTC AACCCAGATG TCCAAACAGC TCTCCCTCTT GGCCAATGGA AGCGAACTTC 
     2221 ACATCGGCAG TGTCCGCTAT GAAGACACCG GAGCATATAC CTGCATTGCC AAGAATGAAG 
     2281 TGGGTGTGGA TGAAGATATC TCCTCACTCT TCATTGAGGA CTCTGCTAGG AAGACATTGG 
     2341 CAAACATCTT GTGGCGGGAA GAAGGCCTCA GCGTGGGAAA CATGTTCTAT GTCTTCGCTG 
     2401 AGGATGGCAT CGTTGTCATC CACCCTGTGG ACTGTGAGGT CCAGAGACGC TTGAAACCTA 
     2461 CAGAGAAGAT CTTCATGAGT TATGAAGAAA TCTGTCCTCG TGTGGAAGGA GATGCTACCC 
     2521 AGCCATGCCA GTGGGCCTCT GCAGTCAATG TCAGAAACCG GTACATCTAC GTGGCCCAGC 
     2581 CAGCACTGAA CAGGGTCCTT GTGGTCGACG TTCAAGCCCA GAAGGTCCTA CAGTCCATAG 
     2641 GTGTGGACCC TCTGCCAGTC AAGCTGTCCT ATGACAAGTC ACATGACCAA GTGTGGGTCC 
     2701 TGAGCTGGGG AGACATGCAC AAGTCCCAAC CAAGCCTACA GGTGATCACA GAAGCCAGCA 
     2761 CTGGCCAAGG CCAGCACCTC ATCCGAACAC CGTTTGCAGG AGTGGACAAT TTCTTTATAC 
     2821 CGCCAACAAA TCTCATCATC AACCATATCA GGTTTGGCTT CATCTTCAAC AAGACTGACC 
     2881 CTGCTGTCCA CAAAGTTGAC CTGGAGACTC TGATGGCACT GAAGACAATC AGCCTACGTC 
     2941 ACTACGGCTG CATGCCTCAG GCCATGGCAC ACACCCACCT TGGTGGCTAC TTCTTCGTGC 
     3001 AGTGTCAACA GGACACACCT ACCTCCACCG GCCCACAGCT GCTCATTGAC AGTGTCACAG 
     3061 ACTCGGTGCT AGGCCCCAAC AGTGATATCA CAGGCACGCC CCACGTGTCC CCTGACGGAC 
     3121 GCTTCATCGT CAGTGTCTCC AACAAGGGCC CCTGGCTGCA CGTGCAGGAG GTCACAGTGC 
     3181 GGGGGGAAAT CCAGACGCTA TACGACCTAA AAATAAACCC AGGCATCTCC GACTTGGCAT 
     3241 TCCAGCATTC CTTCACAGAA GGTAGTCAGT ACAACGCCTA TGCCACTCTC GACAAGGAGC 
     3301 CAGACCTGCT CTTCCTGGAG CTGTCCACTG GGAAGATGGG CAGGCTGAAG AACTTAAAGG 
     3361 AGCCACCCAG AGGGCCAGCC CCAACCTGGG GTGGACCCCG CAGAGTCCTG AGAGACAGCG 
     3421 GGCTCTTTGG ACAGTACCTC CTCACACCAG CTCAAGAGTC ACTGTTCCTG GTCAATGGGA 
     3481 GACAAAACGC CCTGAGGTGT GAGGTTTCAG GCATAAAGGG TGCGGCCACA GTGGTGTGGG 
     3541 TCGGGGAGGT AACGCGTACG CGGCCGCTCG AGATGGAGAG CGACGAGAGC GGCCTGCCCG 
     3601 CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG CGTGGAGTTC GAGCTGGTGG 
     3661 GCGGCGGAGA GGGCACCCCC GAGCAGGGCC GCATGACCAA CAAGATGAAG AGCACCAAAG 
     3721 GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT GGGCTACGGC TTCTACCACT 
     3781 TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA CGCCATCAAC AACGGCGGCT 
     3841 ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT GCTGCACGTG AGCTTCAGCT 
     3901 ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT GATGGGCACC GGCTTCCCCG 
     3961 AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA CGCCACCGTG GAGCACCTGC 
     4021 ACCCCATGGG CGATAACGAT CTGGATGGCA GCTTCACCCG CACCTTCAGC CTGCGCGACG 
     4081 GCGGCTACTA CAGCTCCGTG GTGGACAGCC ACATGCACTT CAAGAGCGCC ATCCACCCCA 
     4141 GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG CGTGGAGGAG GATCACAGCA 
     4201 ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA GACCCCGGAT GCAGATGCCG 
     4261 GTGAAGAAAG AGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4321 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4381 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4441 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4501 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4561 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4621 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4681 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4741 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4801 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4861 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4921 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCTTGGC TGTGGAATGT GTGTCAGTTA 
     4981 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     5041 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5101 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 
     5161 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 
     5221 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 
     5281 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 
     5341 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 
     5401 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 
     5461 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 
     5521 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 
     5581 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 
     5641 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 
     5701 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 
     5761 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 
     5821 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 
     5881 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 
     5941 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 
     6001 GGTGTGGCCG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 
     6061 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 
     6121 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA 
     6181 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT 
     6241 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG 
     6301 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT 
     6361 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 
     6421 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA 
     6481 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 
     6541 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 
     6601 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 
     6661 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 
     6721 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 
     6781 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 
     6841 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 
     6901 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 
     6961 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 
     7021 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 
     7081 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 
     7141 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 
     7201 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 
     7261 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 
     7321 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 
     7381 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 
     7441 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 
     7501 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 
     7561 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 
     7621 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 
     7681 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 
     7741 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 
     7801 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 
     7861 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 
     7921 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 
     7981 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 
     8041 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 
     8101 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 
     8161 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 
     8221 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 
     8281 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 
     8341 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 
     8401 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 
     8461 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 
     8521 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 
     8581 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 
     8641 AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 
     8701 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 
     8761 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG 
     8821 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 
     8881 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 
     8941 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 
     9001 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 
     9061 AACAAAAATT TAACGCGAAT TTT
//