LOCUS       MG220169                9281 bp    DNA     circular  SYN 
DEFINITION  Itih1 (tGFP-tagged) - Mouse inter-alpha trypsin inhibitor heavy chain 1 (Itih1), (10ug)
ACCESSION   NM_008406
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3762..4466 
                     /label="GFP"  
     misc_feature    4513..5098 
                     /label="PolyA signal"  
     misc_feature    7190..7835 
                     /label="ColE1"  
     misc_feature    5552..6346 
                     /label="Neo"  
     misc_feature    5183..5485 
                     /label="SV40 ori"  
     misc_feature    7850..8710 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3741..3747 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3741 
                     /label="Itih1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGGTGCC ACAGGGCTCC GGGTGCTGCT GTGCTTGCTG CCGCTCCTCA 
     1081 CTCTGCAGGC CAGGCCTGCT TTGGGCTTGG CAACAGGCAG ACCCAGGGGC AGTGAGAAGC 
     1141 GACACGCTGT GGACACTTCA GTCAATGGTG TGTCCATCAA GAGCTTGAAA GTCAATTGCA 
     1201 AAGTCACCTC TCGCTTTGCC CACTACGTCA TCACCAGCCA AGTGGTCAAC AATGCTGATA 
     1261 AAGCCAGGGA AGTGGCTTTT GATGTGGAAA TCCCCAAGAC AGCCTTCATC AGTGACTTTG 
     1321 CCATAACATC AGATGGGAAG GCGTTCATTG GGGACATAAA GGACAAAGTG ACTGCATGGA 
     1381 AGCAGTACCG GAAGGCAGCC GTTTTAGGGG AGAGCGCTGG CCTTGTCAGA GCCTCGGGCA 
     1441 GAAATATGGA GCAGTTCACC ATCCATATCA CTGTTGGAGC CCAGAGCAAG GCCACGTTCC 
     1501 GGCTCACCTA TGAGGAGGTA CTAAAGAGGA GACTGATGCA GTATGACATC ACCATCAAAG 
     1561 TCAGACCCAA GCAGCTGGTA CAGCATTTTG AGATCGATGT GGACATCTTT GAACCCCAGG 
     1621 GGATCAGCAA GCTGGACGCT CAGGCCTCCT TCCTCAGTGA GGAACTGGCT GCTCAAACCA 
     1681 TCAAGAAGTC TTTCTCAGGG AAAAAGGGTC ATGTGCTCTT CCGCCCCACT GTGAGCCAGC 
     1741 AGCAGTCCTG CCCCACATGT TCTACATCCT TGCTAAATGG AGAATTCAAG GTGACCTACG 
     1801 ATGTCAATCG AGACAAGCTC TGTGACCTCC TGGTGGCCAA CAACTACTTT ACACATTTCT 
     1861 TTGCCCCCAA AAACCTGACC AACATGAGCA AGAACCTGGT TTTTGTGATT GACATCAGTG 
     1921 GCTCCATGGA AGGCCAGAAA GTGAGGCAGA CAAAGGAGGC ACTCCTTAAG ATCCTGGAGG 
     1981 ACATGAGGCC AGTAGACAAC TTTGACCTGG TCCTCTTTGG CTCTAAAGTG CAATCATGGA 
     2041 AGGGCTCCCT GGTACCTGCG TCTAATGCCA ATCTGCAAGC AGCTCAAGAC TTTGTGAGGC 
     2101 GCTTTTCTTT GGCTGGAGCC ACAAACCTGA ATGGAGGCTT GCTCCGAGGA ATCGAGATCT 
     2161 TAAACAAAGC TCAAGGAAGC CACCCAGAAC TCAGCAGCCC GGCCTCAATT CTTATCATGT 
     2221 TGACAGACGG AGAGCCCACT GAAGGGGAGA CGGACCGTTC CCAGATCCTC AAGAATGTAC 
     2281 GCAACGCTAT CCGGGGCAGG TTCCCGCTCT ACAACCTCGG CTTTGGCCAC GACCTGGACT 
     2341 TCAGCTTCCT GGAGGTCATG TCCACTGAGA ACAACGGATG GGCCCAGAGA ATTTATGAGG 
     2401 ACCATGATGC CACCCAACAG CTACAGGGTT TCTACAATCA AGTAGCCAAT CCCCTGCTGA 
     2461 CAGATGTGGA ACTGCAGTAC CCCCAGGATG CTGTCTTGGC TCTAACCCAG CACCGACATA 
     2521 AACAGTACTA TGACGGCTCG GAGATTGTGG TGGCTGGGCG CATTGCTAAC CACAAACTGA 
     2581 ACACCTTTAA GGCTGACGTT CGGGCTCGTG GGGAAAAGCA AGAATTCAGG GCAACCTGCC 
     2641 TAGTGGACGA GGAAGAGATG AAGAAACTGC TCCGAGAGCG TGGCCACGTG CTAGAGAATC 
     2701 ATGTGGAGAG GCTCTGGGCC TACCTCACGA TCCAGGAGCT CCTGGCTAAG CGGATGAAGA 
     2761 CGGAAGGGGA GGAGAGGGCC AACCTGTCAT CCCAGGTCCT GAAGATGTCA CTGGACTATC 
     2821 ATTTCGTAAC GCCACTGACC TCTCTGACTA TCAGAGGCCT GACAGACGAG GATGGGCTGG 
     2881 AGCCCACCAT TGACAAGCCC CTGGAGGATT CTCAGCCCTT AGAGATGGTG GGACCCAGAA 
     2941 GAACGTTTGT GCTGTCAGCC ATACAGCCAT CTCCTACAGC CCACCCCATA GACTCAAAGT 
     3001 TGCCACTCCG TGTGACAGGT GTGGACACCG ATCCCCACTT CATCATCTAT GTGCCCTCAA 
     3061 AAGAGGATAG CTTGTGCTTC AACATCAATG AGGAACCTGG TGTGATCCTG AACCTAGTAC 
     3121 AGGACCCTGA CACAGGCTTC ACCGTGAATG GGCAGCTCAT TGGGAACAAG GCCAGCAGCC 
     3181 CTGGGCAACA CGAGAGCACA TACTTCGGGA GATTGGGAAT CTCAAGTCCC ACATCAGACT 
     3241 TTCAGCTGGA AGTGACTCCT CAGAACATTA CACTGAACCC CAGCTCCAGC GGGTCCATGT 
     3301 TCTCCTGGAG GGACCAGGCT GTGCTGCAGA AGGATGGGGT AGTGGTGACA ATCAACAAGA 
     3361 AGAGGAACCT GGTGGTGTCT GTGGATGATG GGGCTACCTT TGAGATTGTC CTGCACAGAA 
     3421 CATGGAAGGG GAGTGCAGTC CACCAGGACT TCCTGGGTTT CTATGTGCTG GACAGCTTCC 
     3481 GGATGTCAGC CCGGACAAAG GGGCTACTGG GACAATTCTT CTCCCCACTG GATTTTGAAG 
     3541 TATTTGACCT CCACCCAGGC TCTGACCCTA CAAAGACAGA TGCCACAATG GTGGTGAAGA 
     3601 ATCGGCAGCT GACAGTCACC CGAGGGTTGC AAAAAGACTA CAGCAAGGAC CCCAGACATG 
     3661 GAGCAGAAGT GCCTTGCTGG TTTGTCCACG ACAATGGAGC TGGACTGATT GATGGTGTTC 
     3721 ACACTGACTA TGTCGTCTCT GACATCTTCA CGCGTACGCG GCCGCTCGAG ATGGAGAGCG 
     3781 ACGAGAGCGG CCTGCCCGCC ATGGAGATCG AGTGCCGCAT CACCGGCACC CTGAACGGCG 
     3841 TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG GCACCCCCGA GCAGGGCCGC ATGACCAACA 
     3901 AGATGAAGAG CACCAAAGGC GCCCTGACCT TCAGCCCCTA CCTGCTGAGC CACGTGATGG 
     3961 GCTACGGCTT CTACCACTTC GGCACCTACC CCAGCGGCTA CGAGAACCCC TTCCTGCACG 
     4021 CCATCAACAA CGGCGGCTAC ACCAACACCC GCATCGAGAA GTACGAGGAC GGCGGCGTGC 
     4081 TGCACGTGAG CTTCAGCTAC CGCTACGAGG CCGGCCGCGT GATCGGCGAC TTCAAGGTGA 
     4141 TGGGCACCGG CTTCCCCGAG GACAGCGTGA TCTTCACCGA CAAGATCATC CGCAGCAACG 
     4201 CCACCGTGGA GCACCTGCAC CCCATGGGCG ATAACGATCT GGATGGCAGC TTCACCCGCA 
     4261 CCTTCAGCCT GCGCGACGGC GGCTACTACA GCTCCGTGGT GGACAGCCAC ATGCACTTCA 
     4321 AGAGCGCCAT CCACCCCAGC ATCCTGCAGA ACGGGGGCCC CATGTTCGCC TTCCGCCGCG 
     4381 TGGAGGAGGA TCACAGCAAC ACCGAGCTGG GCATCGTGGA GTACCAGCAC GCCTTCAAGA 
     4441 CCCCGGATGC AGATGCCGGT GAAGAAAGAG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4501 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4561 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4621 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4681 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4741 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4801 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4861 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4921 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4981 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5041 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5101 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCTTGGCTG 
     5161 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5221 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     5281 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 
     5341 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 
     5401 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 
     5461 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 
     5521 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 
     5581 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 
     5641 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 
     5701 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 
     5761 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 
     5821 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 
     5881 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 
     5941 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 
     6001 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 
     6061 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 
     6121 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 
     6181 ATCGACTGTG GCCGGCTGGG TGTGGCCGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 
     6241 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 
     6301 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 
     6361 GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG 
     6421 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 
     6481 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 
     6541 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 
     6601 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 
     6661 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 
     6721 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 
     6781 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 
     6841 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 
     6901 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 
     6961 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 
     7021 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 
     7081 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 
     7141 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 
     7201 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 
     7261 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 
     7321 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 
     7381 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 
     7441 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 
     7501 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 
     7561 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 
     7621 AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 
     7681 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 
     7741 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 
     7801 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 
     7861 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 
     7921 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 
     7981 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 
     8041 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 
     8101 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 
     8161 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 
     8221 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 
     8281 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 
     8341 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 
     8401 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 
     8461 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 
     8521 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 
     8581 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 
     8641 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 
     8701 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     8761 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     8821 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 
     8881 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 
     8941 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 
     9001 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 
     9061 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 
     9121 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 
     9181 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 
     9241 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//