LOCUS       MG219751                9566 bp    DNA     circular  SYN 
DEFINITION  Ano2 (tGFP-tagged) - Mouse anoctamin 2 (Ano2), (10ug)
ACCESSION   NM_153589
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4047..4751 
                     /label="GFP"  
     misc_feature    4798..5383 
                     /label="PolyA signal"  
     misc_feature    7475..8120 
                     /label="ColE1"  
     misc_feature    5837..6631 
                     /label="Neo"  
     misc_feature    5468..5770 
                     /label="SV40 ori"  
     misc_feature    8135..8995 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4026..4032 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4026 
                     /label="Ano2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCCCCT GGGCTGCGAG ACATCCCTCT GCTCCCTGGA TCTCCACGTC 
     1081 GGCTGAGTTC TCGGACCGTG GCCAGAGGAA GCCAGGGCCC CAAACATGGG CAGCAGTACC 
     1141 TCAAAGTGCC AGGTCACCGG GCCCCAGGTC AGAGGGACAA TTCCAGCCTA CACCCTAGCC 
     1201 AGGTCTCCAG GAGAGAGAGT TCCCGAGACC GTTCTGTCAT CAATAACTAC CTGGATGCCA 
     1261 ATGAGCCCCC GTCCTCGGAA GCCCGTCTGA GCCGCATGCA CTTTCACGAC AACCAGAGGA 
     1321 AAGTCGACTA TGTACTTGCA TACCACTACC GGAAACGAGG CGCACACCTG GGTCACGGCT 
     1381 CCCCTGGACA CTCTCTGGCT GTTATCTCCA ATGGGGAGAC AGGCAAGGAG CGCCATGGCG 
     1441 GGGGCCCTGG TGATGTTGAA CTTGGGCCAC TGGATGCTCT GGAAGAGGAA AGGAGGGAGC 
     1501 AGCGAGATGA ATTTGAGCAC AACCTGATGG CTGCTGGGCT GGAGCTGGAG AAGGACTTGG 
     1561 AGAGCAAAAG CCAGGGATCT GTCTTTGTCC GGATCCACGC CCCATGGCAG GTGCTGGCCA 
     1621 GGGAAGCAGA GTTCTTGAAG ATCAAAGTCC CCACCAAGAA AATGTACGAG ATCAAAGCAG 
     1681 GAGGCAGCAT CGCAAAGAAG TTCAGCGCCA TTCTGCAGAC GCTCAGTTCA CCCCTGCAGC 
     1741 CCAGAGTTCC TGAACACAGC AACAACAGGA TGAAAAACCT CTCCTACCCA TTCTCCAGGG 
     1801 AAAAAATGTA CCTGTACAAC ATCCAGGAAA AGGACACCTT CTTTGACAAT GCTACCCGGA 
     1861 GCCGCATTGT ACACGAGATT CTGAAGCGGA CGGCCTGTTC TCGAGCCAAC AACACGATGG 
     1921 GTATTAACTC TCTAATAGCA AACAATATCT ACGAGGCTGC CTACCCGCTT CATGATGGTG 
     1981 AATATGACAG TCCAGGGGAT GACATGAATG ACAGAAAGCT GCTGTATCAG GAATGGGCAC 
     2041 GCTATGGAGT GTTTTACAAA TTTCAACCCA TTGACCTCAT CAGGAAGTAT TTTGGAGAGA 
     2101 AAATTGGGCT ATATTTTGCT TGGCTGGGGT TGTATACATC ATTCCTGATC CCATCTTCTG 
     2161 TCATAGGCGT GATTGTGTTT CTTTATGGAT GTGCAACAAT CGAGGAAGAT ATTCCCAGCA 
     2221 AAGAGATGTG TGACCACCAG AATGCCTTCA CCATGTGTCC CCTGTGTGAC AAGTCCTGTG 
     2281 ACTATTGGAA CCTCAGCTCA GCCTGTGGGA CTGCACGGGC CAGTCATCTG TTTGACAATC 
     2341 CAGCCACCGT CTTCTTTTCC ATCTTCATGG CTCTGTGGGC TACCATGTTT CTGGAAAACT 
     2401 GGAAGAGACT CCAGATGCGG TTGGGCTACT TCTGGGACCT GACTGGGATC GAAGAGGAAG 
     2461 AAGAACGTTC CCAGGAACAC TCTCGGCCTG AATATGAAAC CAAAGTACGA GAGAAACTGT 
     2521 TAAAGGAGAG TGGCAAGTCA GCGGTTCAGA AGCTGGAAGC AAATTCACCT GAGGATGATG 
     2581 AGGATGATGA AGATAAACTG ACTTGGAAAG ATCGTTTCCC AGGATACTTG ATGAACTTTG 
     2641 CCTCCATCTT GTTTATGATT GCCCTGACGT TCTCCATTGT CTTTGGGGTC ATTGTGTATC 
     2701 GGATCACAAC TGCAGCTGCC CTGTCTCTCA ACAAGGCCAC ACGCTCCAAT GTCCGGGTGA 
     2761 CAGTGACTGC AACAGCAGTC ATCATCAACC TCGTGGTCAT CCTCATCTTG GATGAGATCT 
     2821 ATGGTGCTGT GGCCAAGTGG CTTACCAAAA TCGAGGTTCC GAAAACAGAA CAGACTTTTG 
     2881 AAGAACGGTT GATCCTCAAA GCTTTCTTGC TCAAGTTTGT CAATGCCTAC TCCCCCATCT 
     2941 TCTATGTGGC CTTTTTCAAG GGGAGGTTTG TGGGCAGACC TGGAAGCTAT GTCTACGTGT 
     3001 TCGACGGTTA CCGCATGGAA GAGTGTGCTC CGGGAGGCTG TCTCATGGAG CTGTGCATCC 
     3061 AACTCAGCAT CATCATGTTG GGCAAGCAGC TGATCCAGAA CAATATCTTT GAGATTGGAG 
     3121 TCCCGAAGCT AAAGAAACTC TTTCGGAAGC TGAAAGATGA GACAGAGCCT GGAGAAAGTG 
     3181 ACCCTGACCA CTCAAAACGT CCAGAGCAGT GGGACCTGGA CCACAGCCTA GAGCCTTACA 
     3241 CTGGGCTGAC TCCTGAGTAC ATGGAAATGA TCATCCAGTT TGGTTTTGTC ACCCTTTTTG 
     3301 TGGCCTCCTT TCCTCTGGCC CCTGTGTTTG CTCTCCTCAA CAATGTCATT GAAGTGCGGC 
     3361 TGGATGCAAA AAAGTTTGTT ACAGAACTGA GACGCCCAGA TGCTGTGAGG ACCAAAGACA 
     3421 TTGGAATTTG GTTTGACATT CTCTCTGGAA TTGGCAAGTT CTCCGTCATC ATTAATGCTT 
     3481 TTGTCATTGC TGTCACCTCC GACTTTATCC CCCGCCTTGT GTACCAGTAC TCCTATAGCC 
     3541 ACAACGGGAC ACTACATGGC TTTGTGAACC ATACACTCTC CTTTTTCAAC GTCAGTCAGC 
     3601 TGAAGGAGGG AACACAGCCT GAAAACTCAC AGTTCGACCA GGAGGTTCAG TTTTGCAGGT 
     3661 TCAAAGATTA TCGAGAACCA CCATGGGCAC CAAACCCTTA TGAGTTTTCC AAGCAGTACT 
     3721 GGTCTGTTTT GTCTGCCCGT CTGGCCTTTG TTATCATCTT CCAGAACCTA GTGATGTTCC 
     3781 TGAGTGTCCT GGTGGACTGG ATGATTCCGG ATATCCCCAC TGACATCAGC GACCAGATCA 
     3841 AGAAAGAGAA GAGCTTGCTG GTAGACTTCT TTCTGAAGGA GGAGCATGAG AAGGTCAAGC 
     3901 TGGCTGATGA GCCCACCCAG AGGAGCCAGG GAGGTGGGGA TCGGAGCAGG AGGAGCCGTG 
     3961 CAGCCAGCTC TGCACCATCA GGCCGGAGCC AGCCAGGCAG CATAGCATCC TCAGGGTCCC 
     4021 AGCACACCAA TGTAACGCGT ACGCGGCCGC TCGAGATGGA GAGCGACGAG AGCGGCCTGC 
     4081 CCGCCATGGA GATCGAGTGC CGCATCACCG GCACCCTGAA CGGCGTGGAG TTCGAGCTGG 
     4141 TGGGCGGCGG AGAGGGCACC CCCGAGCAGG GCCGCATGAC CAACAAGATG AAGAGCACCA 
     4201 AAGGCGCCCT GACCTTCAGC CCCTACCTGC TGAGCCACGT GATGGGCTAC GGCTTCTACC 
     4261 ACTTCGGCAC CTACCCCAGC GGCTACGAGA ACCCCTTCCT GCACGCCATC AACAACGGCG 
     4321 GCTACACCAA CACCCGCATC GAGAAGTACG AGGACGGCGG CGTGCTGCAC GTGAGCTTCA 
     4381 GCTACCGCTA CGAGGCCGGC CGCGTGATCG GCGACTTCAA GGTGATGGGC ACCGGCTTCC 
     4441 CCGAGGACAG CGTGATCTTC ACCGACAAGA TCATCCGCAG CAACGCCACC GTGGAGCACC 
     4501 TGCACCCCAT GGGCGATAAC GATCTGGATG GCAGCTTCAC CCGCACCTTC AGCCTGCGCG 
     4561 ACGGCGGCTA CTACAGCTCC GTGGTGGACA GCCACATGCA CTTCAAGAGC GCCATCCACC 
     4621 CCAGCATCCT GCAGAACGGG GGCCCCATGT TCGCCTTCCG CCGCGTGGAG GAGGATCACA 
     4681 GCAACACCGA GCTGGGCATC GTGGAGTACC AGCACGCCTT CAAGACCCCG GATGCAGATG 
     4741 CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4801 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4861 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4921 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4981 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5041 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5101 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5161 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5221 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5281 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5341 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5401 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG 
     5461 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     5521 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5581 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 
     5641 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 
     5701 GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 
     5761 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 
     5821 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 
     5881 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 
     5941 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 
     6001 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 
     6061 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 
     6121 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 
     6181 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 
     6241 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 
     6301 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 
     6361 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 
     6421 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 
     6481 CTGGGTGTGG CCGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG 
     6541 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG 
     6601 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG 
     6661 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT 
     6721 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC 
     6781 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG 
     6841 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT 
     6901 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT 
     6961 CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC 
     7021 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT 
     7081 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 
     7141 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 
     7201 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 
     7261 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 
     7321 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 
     7381 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 
     7441 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 
     7501 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 
     7561 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 
     7621 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 
     7681 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 
     7741 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 
     7801 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 
     7861 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 
     7921 GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 
     7981 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 
     8041 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 
     8101 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 
     8161 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 
     8221 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 
     8281 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 
     8341 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 
     8401 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 
     8461 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 
     8521 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 
     8581 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 
     8641 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 
     8701 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 
     8761 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 
     8821 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 
     8881 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 
     8941 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 
     9001 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 
     9061 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 
     9121 GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA 
     9181 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC 
     9241 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT 
     9301 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG 
     9361 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA 
     9421 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT 
     9481 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA 
     9541 TTTAACAAAA ATTTAACGCG AATTTT
//