LOCUS       MG219497                9413 bp    DNA     circular  SYN 
DEFINITION  Lgr4 (tGFP-tagged) - Mouse leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4), (10ug)
ACCESSION   NM_172671
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3894..4598 
                     /label="GFP"  
     misc_feature    4645..5230 
                     /label="PolyA signal"  
     misc_feature    7322..7967 
                     /label="ColE1"  
     misc_feature    5684..6478 
                     /label="Neo"  
     misc_feature    5315..5617 
                     /label="SV40 ori"  
     misc_feature    7982..8842 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3873..3879 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3873 
                     /label="Lgr4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGGCCCG CTGCGGCTGC TCTGCTTCTT CGCCCTGGGG CTGCTCGGCT 
     1081 CGGCCGGGCC CAGCGGCGCG GCGCCACCTC TCTGCGCTGC GCCCTGCAGC TGCGACGGCG 
     1141 ACCGTCGGGT GGACTGCTCC GGGAAGGGGT TGACGGCGGT ACCGGAGGGG CTCAGCGCCT 
     1201 TCACCCAAGC ACTGGATATC AGTATGAACA ATATCACTCA GTTACCAGAA GATGCATTTA 
     1261 AGAATTTTCC TTTTCTAGAG GAGCTACAAC TGGCTGGTAA CGACCTTTCT TTTATCCACC 
     1321 CAAAAGCCTT GTCTGGGTTG AAAGAACTCA AAGTCCTAAC CCTCCAGAAC AATCAGTTGA 
     1381 AAACAGTACC CAGTGAAGCC ATTCGTGGAC TGAGTGCTTT GCAGTCTCTA CGCTTAGATG 
     1441 CCAACCATAT TACCTCAGTC CCGGAGGACA GTTTTGAAGG GCTCGTTCAG TTGCGGCATC 
     1501 TGTGGCTGGA TGACAACATC TTGACGGAAG TGCCTGTGCG TCCGCTCAGC AACCTGCCAA 
     1561 CCCTGCAGGC GCTGACCTTG GCTCTCAACA ACATCTCAAG CATCCCCGAC TTCGCATTCA 
     1621 CCAACCTTTC AAGCTTGGTA GTGCTGCATC TTCATAACAA TAAAATTAAA AGCCTCAGTC 
     1681 AACACTGTTT TGATGGACTA GATAACCTGG AAACCCTGGA CTTGAATTAT AATAACTTGG 
     1741 ATGAATTTCC TCAGGCTATT AAAGCCCTTC CCAGCCTTAA AGAGCTGGGA TTTCACAGTA 
     1801 ATTCTATTTC TGTTATCCCG GATGGAGCAT TTGCTGGTAA TCCACTGCTA AGAACTATCC 
     1861 ATTTGTATGA TAATCCTCTG TCTTTTGTGG GGAACTCAGC ATTTCACAAC CTGTCTGATC 
     1921 TGCATTCCTT AGTCATTCGT GGTGCAAGCC TGGTGCAGTG GTTCCCCAAT CTGGCCGGAA 
     1981 CTGTCCATCT GGAGAGTCTA ACCTTGACAG GGACAAAAAT AAGCAGCATA CCTGATGATC 
     2041 TGTGCCAAAA CCAAAAGATG CTGCGGACTC TGGACTTATC TTATAACGAT ATAAGAGACC 
     2101 TTCCAAGTTT TAATGGTTGT CGTGCATTGG AAGAAATTTC ATTGCAGCGT AATCAAATCT 
     2161 CCCTGATAAA GGAAACTACT TTTCAAGGCC TAACATCCCT AAGGATTCTA GATCTGAGTA 
     2221 GAAACCTGAT TCGTGAAATT CACAGTGGAG CTTTTGCGAA GCTTGGGACA ATTACTAACC 
     2281 TGGATGTGAG TTTCAATGAA TTAACCTCAT TTCCTACGGA AGGCCTGAAT GGGCTCAATC 
     2341 AACTTAAACT TGTGGGTAAC TTCCAGCTGA AAGATGCCTT GGCAGCCAGA GACTTTGCCA 
     2401 ATCTCAGGTC TCTATCAGTA CCATATGCTT ATCAGTGTTG TGCATTTTGG GGGTGTGACT 
     2461 CTTATGCAAA TTTAAACACA GAAGATAACA GCCCCCAAGA CCACAGTGTG ACAAAAGAGA 
     2521 AAGGTGCTAC AGATGCAGCA AATGCCACCA GCACTGCTGA AAGTGAAGAA CATAGCCAAA 
     2581 TAATCATCCA TTGTACACCT TCAACAGGTG CTTTTAAGCC CTGTGAATAT TTACTGGGAA 
     2641 GCTGGATGAT TCGCCTTACA GTGTGGTTCA TTTTCCTGGT CGCCTTGCTT TTCAACCTGC 
     2701 TTGTCATTTT AACAGTGTTT GCGTCTTGTT CATCACTGCC TGCCTCCAAG CTCTTCATAG 
     2761 GCTTGATTTC TGTGTCTAAC TTACTCATGG GCATCTATAC TGGCATCCTT ACTTTTCTTG 
     2821 ATGCTGTGTC CTGGGGCCGA TTTGCTGAAT TTGGCATTTG GTGGGAAACT GGCAGCGGCT 
     2881 GCAAGGTAGC TGGGTCTCTG GCAGTCTTCT CCTCAGAGAG CGCCGTATTC CTGTTAACCC 
     2941 TGGCAGCCGT GGAAAGAAGC GTTTTTGCAA AGGATGTAAT GAAAAATGGG AAAAGCAGTC 
     3001 ACCTCCGACA GTTCCAGGTG GCTGCCCTCG TAGCTTTGCT GGGTGCTGCA ATAGCAGGCT 
     3061 GCTTCCCCCT TTTTCACGGA GGGCAATATT CTGCATCACC CTTGTGCTTG CCATTTCCTA 
     3121 CAGGAGAGAC ACCATCATTA GGATTCACTG TGACCCTAGT GCTATTAAAC TCACTAGCAT 
     3181 TTTTATTGAT GGCCATTATC TACACTAAAC TCTACTGCAA CTTAGAGAAA GAAGACCCGT 
     3241 CAGAAAACTC CCAGTCTAGC ATGATTAAGC ACGTTGCTTG GCTCATCTTC ACAAACTGCA 
     3301 TCTTCTTCTG CCCTGTTGCA TTTTTCTCAT TCGCACCATT GATCACGGCA ATCTCCATCA 
     3361 GCCCCGAGAT AATGAAGTCT GTTACGCTGA TATTCTTCCC GCTGCCTGCT TGCCTGAATC 
     3421 CAGTCCTGTA CGTTTTCTTC AACCCAAAGT TTAAAGACGA CTGGAAGCTC CTGAAGCGGC 
     3481 GTGTCACCAG GAAACACGGA TCAGTCTCAG TCTCCATCAG CAGCCAAGGC GGTTGTGGGG 
     3541 AACAGGATTT CTACTACGAC TGTGGCATGT ATTCCCACTT GCAGGGTAAC CTGACTGTCT 
     3601 GTGACTGCTG TGAGTCATTT CTTCTGACAA AACCAGTATC GTGCAAACAC TTAATAAAAT 
     3661 CGCACAGTTG TCCTGTATTG ACAGTGGCCT CTTGCCAGAG GCCAGAGGCC TACTGGTCTG 
     3721 ATTGTGGCAC ACAGTCGGCC CATTCTGACT ATGCAGATGA AGAGGATTCC TTTGTCTCGG 
     3781 ACAGCTCTGA CCAGGTGCAG GCCTGTGGAC GAGCCTGCTT CTACCAGAGT CGCGGATTCC 
     3841 CTCTGGTGCG CTATGCTTAT AATCTACCGA GAGTCAGAGA CACGCGTACG CGGCCGCTCG 
     3901 AGATGGAGAG CGACGAGAGC GGCCTGCCCG CCATGGAGAT CGAGTGCCGC ATCACCGGCA 
     3961 CCCTGAACGG CGTGGAGTTC GAGCTGGTGG GCGGCGGAGA GGGCACCCCC GAGCAGGGCC 
     4021 GCATGACCAA CAAGATGAAG AGCACCAAAG GCGCCCTGAC CTTCAGCCCC TACCTGCTGA 
     4081 GCCACGTGAT GGGCTACGGC TTCTACCACT TCGGCACCTA CCCCAGCGGC TACGAGAACC 
     4141 CCTTCCTGCA CGCCATCAAC AACGGCGGCT ACACCAACAC CCGCATCGAG AAGTACGAGG 
     4201 ACGGCGGCGT GCTGCACGTG AGCTTCAGCT ACCGCTACGA GGCCGGCCGC GTGATCGGCG 
     4261 ACTTCAAGGT GATGGGCACC GGCTTCCCCG AGGACAGCGT GATCTTCACC GACAAGATCA 
     4321 TCCGCAGCAA CGCCACCGTG GAGCACCTGC ACCCCATGGG CGATAACGAT CTGGATGGCA 
     4381 GCTTCACCCG CACCTTCAGC CTGCGCGACG GCGGCTACTA CAGCTCCGTG GTGGACAGCC 
     4441 ACATGCACTT CAAGAGCGCC ATCCACCCCA GCATCCTGCA GAACGGGGGC CCCATGTTCG 
     4501 CCTTCCGCCG CGTGGAGGAG GATCACAGCA ACACCGAGCT GGGCATCGTG GAGTACCAGC 
     4561 ACGCCTTCAA GACCCCGGAT GCAGATGCCG GTGAAGAAAG AGTTTAAACG GCCGGCCGCG 
     4621 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4681 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4741 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4801 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4861 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4921 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4981 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5041 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5101 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5161 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5221 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5281 CCAGCTTGGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     5341 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA 
     5401 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC 
     5461 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC 
     5521 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA 
     5581 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA 
     5641 GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT 
     5701 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT 
     5761 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG 
     5821 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC 
     5881 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC 
     5941 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC 
     6001 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG 
     6061 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG 
     6121 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT 
     6181 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG 
     6241 GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC 
     6301 TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCCG ACCGCTATCA GGACATAGCG 
     6361 TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG 
     6421 CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG 
     6481 TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT 
     6541 CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC 
     6601 GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC 
     6661 CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA 
     6721 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT 
     6781 CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC 
     6841 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA 
     6901 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 
     6961 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 
     7021 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 
     7081 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 
     7141 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 
     7201 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 
     7261 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 
     7321 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 
     7381 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 
     7441 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 
     7501 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 
     7561 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 
     7621 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG 
     7681 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 
     7741 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 
     7801 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 
     7861 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 
     7921 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 
     7981 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC 
     8041 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC 
     8101 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT 
     8161 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG 
     8221 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA 
     8281 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA 
     8341 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT 
     8401 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG 
     8461 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA 
     8521 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC 
     8581 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT 
     8641 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC 
     8701 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA 
     8761 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA 
     8821 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 
     8881 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 
     8941 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG 
     9001 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC 
     9061 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC 
     9121 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG 
     9181 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG 
     9241 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG 
     9301 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT 
     9361 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//