LOCUS       MG218168                9353 bp    DNA     circular  SYN 
DEFINITION  Sik2 (tGFP-tagged) - Mouse salt inducible kinase 2 (Sik2), (10ug)
ACCESSION   NM_178710
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3834..4538 
                     /label="GFP"  
     misc_feature    4585..5170 
                     /label="PolyA signal"  
     misc_feature    7262..7907 
                     /label="ColE1"  
     misc_feature    5624..6418 
                     /label="Neo"  
     misc_feature    5255..5557 
                     /label="SV40 ori"  
     misc_feature    7922..8782 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3813..3819 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3813 
                     /label="Sik2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCATGGCG GATGGCCCGA GGCACTTGCA GCGCGGGCCA GTCCGGGTGG 
     1081 GGTTCTACGA CATCGAGGGC ACGCTGGGCA AGGGCAACTT TGCCGTGGTG AAGCTGGGGC 
     1141 GGCACCGGAT CACCAAGACG GAGGTGGCTA TAAAAATAAT AGACAAGTCA CAGCTGGATG 
     1201 CAGTAAACCT TGAGAAAATC TACCGAGAAG TACAGATAAT GAAAATGCTC GACCATCCTC 
     1261 ACATCATTAA ACTGTATCAG GTAATGGAGA CCAAAAGTAT GTTGTACCTT GTGACAGAAT 
     1321 ATGCCAAAAA TGGAGAAATT TTTGATTATC TTGCTAATCA TGGCCGATTA AATGAATCTG 
     1381 AAGCCAGGAG AAAATTCTGG CAAATTCTGT CTGCTGTTGA TTATTGCCAT GGCCGGAAGG 
     1441 TAGTGCACAG AGACCTGAAG GCTGAAAATC TCCTGCTGGA TAACAACATG AATATCAAAA 
     1501 TAGCAGATTT CGGCTTTGGA AATTTCTTTA AAACTGGTGA ACTGCTGGCA ACATGGTGTG 
     1561 GCAGCCCCCC TTATGCAGCC CCAGAAGTCT TTGAAGGGCA GCAGTATGAA GGACCACAGC 
     1621 TGGATATATG GAGCATGGGA GTTGTTCTTT ATGTCCTTGT CTGTGGAGCT CTACCTTTTG 
     1681 ATGGACCAAC TCTCCCTATT TTGAGGCAGA GGGTTTTAGA AGGAAGATTC CGGATTCCTT 
     1741 ATTTCATGTC AGAAGATTGT GAACACCTCA TTAGAAGGAT GTTGGTCCTA GATCCTTCCA 
     1801 AACGGCTAAG CATAGCTCAA ATCAAGGAGC ACAAGTGGAT GCTCATAGAG GTTCCTGTAC 
     1861 AGAGGCCTAT TCTCTACCCA CAAGAACAGG AAAACGAGCC GTCCATTGGA GAATTTAATG 
     1921 AGCAGGTTCT TCGACTGATG CACAGCCTTG GGATTGATCA GCAGAAGACT GTTGAGTCTT 
     1981 TGCAAAACAA GAGCTATAAT CACTTTGCTG CCATTTATTT CTTGTTGGTG GAACGTCTAA 
     2041 AATCACATAG GAGCAGTTTT CCTGTGGAGC AGAGACTTGA TGGCCGCCAG CGTCGGCCTA 
     2101 GCACCATTGC TGAACAAACA GTTGCCAAGG CACAAACTGT GGGGCTGCCA GTGACCTTGC 
     2161 ATCCACCGAA CGTGAGACTG ATGCGATCTA CCCTCCTCCC ACAGGCATCC AATGTGGAGG 
     2221 CCTTTTCATT TCCAACATCC AGCTGTCAAG CAGAAGCTGC CTTTATGGAG GAAGAGTGTG 
     2281 TCGACACTCC AAAGGTGAAT GGCTGCCTGC TTGACCCTGT GCCCCCTGTC CTGGTGAGGA 
     2341 AGGGATGCCA GTCACTGCCC AGCAGTATGA TGGAGACCTC CATTGATGAA GGCTTGGAGA 
     2401 CAGAAGGAGA GGCTGAGGAA GACCCCAGTC AGGCCTTTGA AGCTTTCCAG GCCACACGCA 
     2461 GTGGGCAGCG ACGGCACACT CTGTCAGAAG TGACTAACCA ATTGGTTGTG ATGCCCGGGG 
     2521 CAGGGAAAAT GTTCTCCATG AGTGATAACC CCTCCCTTGA GAGTGTGGAC TCTGAGTATG 
     2581 ATATGGGGTC TGCCCAGAGG GACCTGAACT TTCTGGAAGA CAGCCCTTCC TTGAAGGACA 
     2641 TCATGTTAGC CAATCAGCCG TCACCCCGCA TGACATCTCC TTTCATAAGC CTCAGACCTG 
     2701 CCAACCCAGC CATGCAGGCT CTGAGCTCAC AGAAGCGTGA GGCCCACAAC CGGTCCCCCG 
     2761 TGAGCTTCCG AGAAGGCCGC AGAGCATCAG ATACGTCCCT TACACAAGGA ATTGTAGCAT 
     2821 TTAGACAACA TCTTCAGAAT CTTGCTAGAA CCAAAGGAAT TCTGGAGTTG AACAAAGTAC 
     2881 AATTGCTGTA TGAACAAATG GGATCAAACG CAGACCCTAC CTTAACATCA ACTGCTCCTC 
     2941 AGCTCCAAGA CCTTTCGAGC AGTTGCCCTC AGGAGGAAAT CTCCCAGCAG CAGGAAAGTG 
     3001 TCTCCAGCCT GTCTGCCAGC ATGCACCCTC AGCTCTCACC ACAGCAAAGC TTGGAAACCC 
     3061 AGTACCTACA GCATCGACTC CAGAAGCCCA ATCTTCTGCC AAAGGCCCAG AGTCCTTGTC 
     3121 CAGTGTATTG TAAAGAGCCA CCTCGGAGCC TGGAACAGCA GCTACAGGAG CATAGGCTCC 
     3181 AACAGAAGCG ACTCTTCCTC CAGAAGCAGT CTCAGCTGCA AGCATATTTT AATCAGATGC 
     3241 AGATAGCAGA GAGCTCCTAC CCTGGACCAA GTCAGCAGCT GGCTCTTCCC CACCAGGAGA 
     3301 CTCCACTGAC ATCCCAGCAG CCCCCATCAT TCAGCCTGAC CCAGGCCCTG AGTCCTGTCC 
     3361 TCGAGCCCTC TTCTGAGCAG ATGCAATTTA GCTCTTTCCT CAGCCAATAC CCAGAGATGC 
     3421 AGTTGCAGCC ACTGCCCTCT ACTCCCGGCC CCCAGGCTCC ACCTCCCTTA CCCTCACAAT 
     3481 TGCAGCAACA TCAGCAGCCA CCACCCCCAC CACCACCCCC TCCACCACAG CAGCCAGGAG 
     3541 CTGCTCCAAC CTCCTTACAG TTCTCCTATC AGACTTGTGA GCTGCCAAGC ACCACTTCTT 
     3601 CTGTACCAAA CTATCCTGCT TCCTGTCACT ATCCTGTGGA TGGAGCCCAG CAGAGCAACC 
     3661 TCACAGGGGC GGACTGTCCC AGGAGCTCAG GACTTCAGGA CACCGCATCT AGCTATGACC 
     3721 CACTGGCCCT CTCTGAGCTC CCTGGACTCT TTGATTGTGA AATGGTAGAA GCTGTGGATC 
     3781 CACAACACAA TGGGGTTGTG AGCTGCTTAG CCCGGGAGAC CACGCGTACG CGGCCGCTCG 
     3841 AGATGGAGAG CGACGAGAGC GGCCTGCCCG CCATGGAGAT CGAGTGCCGC ATCACCGGCA 
     3901 CCCTGAACGG CGTGGAGTTC GAGCTGGTGG GCGGCGGAGA GGGCACCCCC GAGCAGGGCC 
     3961 GCATGACCAA CAAGATGAAG AGCACCAAAG GCGCCCTGAC CTTCAGCCCC TACCTGCTGA 
     4021 GCCACGTGAT GGGCTACGGC TTCTACCACT TCGGCACCTA CCCCAGCGGC TACGAGAACC 
     4081 CCTTCCTGCA CGCCATCAAC AACGGCGGCT ACACCAACAC CCGCATCGAG AAGTACGAGG 
     4141 ACGGCGGCGT GCTGCACGTG AGCTTCAGCT ACCGCTACGA GGCCGGCCGC GTGATCGGCG 
     4201 ACTTCAAGGT GATGGGCACC GGCTTCCCCG AGGACAGCGT GATCTTCACC GACAAGATCA 
     4261 TCCGCAGCAA CGCCACCGTG GAGCACCTGC ACCCCATGGG CGATAACGAT CTGGATGGCA 
     4321 GCTTCACCCG CACCTTCAGC CTGCGCGACG GCGGCTACTA CAGCTCCGTG GTGGACAGCC 
     4381 ACATGCACTT CAAGAGCGCC ATCCACCCCA GCATCCTGCA GAACGGGGGC CCCATGTTCG 
     4441 CCTTCCGCCG CGTGGAGGAG GATCACAGCA ACACCGAGCT GGGCATCGTG GAGTACCAGC 
     4501 ACGCCTTCAA GACCCCGGAT GCAGATGCCG GTGAAGAAAG AGTTTAAACG GCCGGCCGCG 
     4561 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4621 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4681 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4741 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4801 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4861 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4921 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4981 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5041 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5101 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5161 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5221 CCAGCTTGGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     5281 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA 
     5341 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC 
     5401 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC 
     5461 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA 
     5521 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA 
     5581 GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT 
     5641 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT 
     5701 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG 
     5761 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC 
     5821 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC 
     5881 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC 
     5941 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG 
     6001 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG 
     6061 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT 
     6121 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG 
     6181 GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC 
     6241 TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCCG ACCGCTATCA GGACATAGCG 
     6301 TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG 
     6361 CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG 
     6421 TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT 
     6481 CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC 
     6541 GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC 
     6601 CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA 
     6661 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT 
     6721 CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC 
     6781 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA 
     6841 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 
     6901 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 
     6961 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 
     7021 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 
     7081 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 
     7141 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 
     7201 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 
     7261 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 
     7321 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 
     7381 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 
     7441 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 
     7501 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 
     7561 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG 
     7621 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 
     7681 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 
     7741 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 
     7801 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 
     7861 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 
     7921 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC 
     7981 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC 
     8041 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT 
     8101 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG 
     8161 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA 
     8221 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA 
     8281 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT 
     8341 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG 
     8401 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA 
     8461 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC 
     8521 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT 
     8581 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC 
     8641 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA 
     8701 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA 
     8761 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 
     8821 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 
     8881 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG 
     8941 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC 
     9001 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC 
     9061 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG 
     9121 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG 
     9181 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG 
     9241 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT 
     9301 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//