LOCUS MG216937 7709 bp DNA circular SYN DEFINITION Als2cr12 (tGFP-tagged) - Mouse amyotrophic lateral sclerosis 2 (juvenile) chromosome region candidate 12 (human) (Als2cr12), (10ug) ACCESSION NM_175370 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 2190..2894 /label="GFP" misc_feature 2941..3526 /label="PolyA signal" misc_feature 5618..6263 /label="ColE1" misc_feature 3980..4774 /label="Neo" misc_feature 3611..3913 /label="SV40 ori" misc_feature 6278..7138 /label="Amp" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 2169..2175 /label="Restriction Enzyme" misc_feature 1020..2169 /label="Flacc1" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGACCCCAAT CCTTTCATCT ACTGTACCTG CTGTGACTGC TGCAAATTGG 1081 GACAACCAAA GCTAATCAAG ACCCCTTATC CACTTCCAAA GCACCCCACG GGGAAGTTTA 1141 AGCCAGTTTT ACCTCCACCA ATTTCAAAAG AACACAACTC ACTCCTGTCC CAACCAGGAA 1201 AATCTACTGT TTCCCCAAGG GACAAGGTCC AGTCAGGAAA CACAGAGAGC AGCAAAGCAC 1261 CTTCTGAAGT GATTCAAGTG TCACCTGGCT ATACACTCAT TCGAAATCGA GAACAGATAT 1321 CAGTCACCTT GGGAGATGAG ATGTTCAACA GGAAAAAGCA CTTGGAATCA GATGTCTTGA 1381 GCAAAGTCAA ATTTTCCAGG ACTGATATCA TTTCTGACCT TCAGGAGCAA ATTGCTGAGT 1441 TGATGGCAAT AATAGAACAG ATGAACAGAG ACCAGCAGTC TGCCCTGAAA CTGCTCTCCA 1501 AAGATCTGGA TCTCCGCTGT TCCAACATGA AGCAGAAGTT TGAAACCGAG AGCAGGGAAC 1561 TCAAAGAGAC TCACAGAGAG GAGCTCGAGA GGTTAGAGAA CAACTACAAG GAGGCCCTGA 1621 AGGCAGAAAA GGCCTTGGCC GAAGAGAAAC TAGATAAGAT GAGCAAGGAA TATAAGTATC 1681 TGAAGAGTAT GTTCCATGTG TTTCAGGATA GCATTTACGA GGAAATGGAA GACAAGTGGC 1741 TGAGACGAAA GGCCGAATGG GAGAAAGATG AGAAGATGGA GCGGGAAAAG ATCCTGCTAC 1801 AACAAAAATG TAGAATAATA AAAAAGTTTG AGTTACAGTC GGAAGAAAAA AAGAAGAAAA 1861 TGAATGAGTC CATCAGTGCT GTTTCTGATA ACTTCGCTCG AGAGAAAGAG GAGCTCCTAA 1921 GACAACATGA TGAAGACATC CTGCAAATAC AGGAGCTGAG AAAATCCAAA GAGATCTTGG 1981 AGGCTGAGTT GCGGGCACAA GCTACTGTTC TGGAGACCCT GAACACAAAC CTTTTCCAAT 2041 GCCAGAAAGA ACTCGAGAGA CAGAAAACTA TTGCGGCAAA TCTAGAGAAA TTGTTCCAGA 2101 CAAAGCTTGC TGAAGCTGAA GAAAAGCATA AGTACAACAT AAAGACCCCG ACAGAAGAAA 2161 ATAACTGCCT CAGATGTACG CGTACGCGGC CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC 2221 TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC 2281 TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA 2341 CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT 2401 ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG 2461 GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT 2521 TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGATG GGCACCGGCT 2581 TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC 2641 ACCTGCACCC CATGGGCGAT AACGATCTGG ATGGCAGCTT CACCCGCACC TTCAGCCTGC 2701 GCGACGGCGG CTACTACAGC TCCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC 2761 ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGGATC 2821 ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCGGATGCAG 2881 ATGCCGGTGA AGAAAGAGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 2941 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 3001 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 3061 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 3121 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 3181 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 3241 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 3301 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 3361 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 3421 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 3481 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 3541 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CTTGGCTGTG GAATGTGTGT 3601 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 3661 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 3721 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 3781 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 3841 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 3901 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 3961 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 4021 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 4081 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 4141 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 4201 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 4261 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 4321 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 4381 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 4441 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 4501 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 4561 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 4621 CGGCTGGGTG TGGCCGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 4681 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 4741 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 4801 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 4861 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 4921 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 4981 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 5041 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 5101 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 5161 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 5221 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 5281 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 5341 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 5401 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 5461 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 5521 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 5581 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 5641 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 5701 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 5761 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 5821 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 5881 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 5941 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 6001 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 6061 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 6121 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 6181 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 6241 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 6301 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 6361 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 6421 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 6481 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 6541 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 6601 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 6661 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 6721 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 6781 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 6841 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 6901 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 6961 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 7021 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 7081 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 7141 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 7201 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 7261 CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 7321 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 7381 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC 7441 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 7501 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 7561 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 7621 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC 7681 TGATTTAACA AAAATTTAAC GCGAATTTT //