LOCUS       MG216857                9465 bp    DNA     circular  SYN 
DEFINITION  Rasip1 (tGFP-tagged) - Mouse Ras interacting protein 1 (Rasip1), (10ug)
ACCESSION   NM_028544
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3947..4651 
                     /label="GFP"  
     misc_feature    4698..5283 
                     /label="PolyA signal"  
     misc_feature    7375..8020 
                     /label="ColE1"  
     misc_feature    5737..6531 
                     /label="Neo"  
     misc_feature    5368..5670 
                     /label="SV40 ori"  
     misc_feature    8035..8895 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3903..3909 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3903 
                     /label="Rasip1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCATG CTATCTGGTG AACGAAAGGA GGGCGGAAGC CCCCGCTTCG GGAAGCTCCA 
     1081 TCTTCCAGTG GGCCTGTGGA TCAATTCCCC AAGGAAACAG CTCGCCAAAC TGGGGCGGCG 
     1141 CTGGCCCAGC GCTGCCTCTG TCAAGTCCTC GTCCTCCGAC ACGGGGAGCC GAAGCAGCGA 
     1201 GCCCCTGCCG CCGCCGCCGC CGCCGCCGCA CGTGGAGCTG CGGCGCGTAG GTGCGGTGAA 
     1261 GGCGGCGGGG GGAGCGTCGG GCAGCCGAGC CAAGCGCATC TCGCAGCTGT TTCGGGGCTC 
     1321 AGGGGCCGGC GGCGCCGGGG GTCCTGGCAC GCCTGGGGGC GCGCAGCGCT GGGCCAGCGA 
     1381 GAAGAAGCTG CCTGAACTGG CGGCGGGTGT AGCTCCGGAG CCCCCGTTGC CCACGCGTGC 
     1441 CGCGGTGCCC CCGGGAGTGC TCAAGATCTT CGCTTCTGGG CTGGCGTCGG GCGCCAACTA 
     1501 CAAGAGCGTG CTGGCTACGG AGCGATCCAC CGCGCGCGAG CTGGTGGCGG AGGCGCTGGA 
     1561 GCGCTACGGG CTGACGGGCG GCCGCGGAGC GGGCGACAGC GGCTGCGTGG ACGCCTACGC 
     1621 GCTTTGCGAC GCTCTGGGCC GGCCCGCGGT CGGCGTGGGC GGAGGCGAGT GGCGTGCCGA 
     1681 GCACCTGCGG GTGTTGGCCG ACGCGGAGCG CCCTCTGCTG GTGCAGGACC TGTGGCGCGC 
     1741 CCGTCCGGGC TGGGCGCGGC GCTTTGAGCT GCGCGGCCGC GAGGAGGCTC GACGGCTGGA 
     1801 GCAGGAGGCC TTCGGGGCTG CGGATGCAGA CGGCACAAAC GCCCCATCTT GGCGGACCCA 
     1861 GAAGAATAGG TCTCGGGCAG CATCTGGCGG GGCAGCTCTG GCCAGTCCTG GTCCAGGATC 
     1921 AGGATCCGGG ACCCCCACTG GGTCCGGGGG CAAGGAGCGA TCTGAAAATT TGTCTCTGCG 
     1981 TCGAAGCGTG TCTGAGCTGA GTCTACAGGG GCGGAGGCGA AGGCAGCAGG AACGCAGGCA 
     2041 GCAGGCCCTT AGCATGGCCC CAGGGGCAGC TGATGCTCAG ATGGTACCAA CGGACCCTGG 
     2101 AGACTTCGAT CAGTTGACTC AGTGCCTCAT TCAGGCACCC AGCAACCGTC CCTACTTCCT 
     2161 TCTGCTCCAG GGCTACCAGG ATGCTCAGGA CTTCGTGGTG TATGTCATGA CACGAGAGCA 
     2221 GCACGTGTTC GGCCGGGGTG GGCCCTCCTC GTCCCGCGGG GGATCCCCAG CCCCATATGT 
     2281 GGACACCTTC CTCAACGCCC CAGACATCCT GCCTCGACAT TGCACGGTGC GCGCGGGCCC 
     2341 TGAGCCCCCG GCCATGGTGC GCCCATCCCG GGGCGCCCCC GTCACACACA ATGGCTGTCT 
     2401 TTTGCTGCGG GAGGCAGAGC TGCACCCCGG AGACCTGTTA GGCCTAGGAG AGCATTTCCT 
     2461 GTTCATGTAC AAGGACCCCC GCTCCGGGGG TTCTGGGCCC GCTCGGCCAT CTTGGCTGCC 
     2521 AGCGCGCCCC GGGGCCGCGC CACCCGGACC GGGCTGGGCC TTTTCTTGTC GTTTGTGTGG 
     2581 CCGTGGCCTG CAGGAGCGCG GCGAGGCGCT GGCCGCCTAC CTGGATGGCC GCGAGCCGGT 
     2641 GCTGCGGTTC AGACCACGAG AGGAAGAGGC GCTGTTGGGC GAGATCGTGC GAGCTGCAGC 
     2701 CTCGGGAGCA GGGGACCTGC CCCCGCTCGG GCCAGCCACT CTGCTGGCAC TGTGTGTGCA 
     2761 GCATTCTGCG AGGGAGCTGG AGCTGGGCCA CCTGCCTCGC TTGCTGGGCC GCCTGGCCCG 
     2821 GCTCATCAAA GAAGCCGTTT GGGAAAAAAT TAAAGAAATT GGAGACCGCC AGCCAGAGAA 
     2881 CCACCCAGAG GGAGTTCCCG AGGTGCCTCT GACCCCTGAG GCTGTGTCTG TGGAGCTGCG 
     2941 ACCACTCATA CTATGGATGG CCAACACCAC AGAGCTGTTG AGCTTTGTAC AGGAGAAAGT 
     3001 GCTAGAGATG GAGAAGGAGG CTGACCAGGA GGGCCTGTCC TCGGACCCAC AGCTCTGCAA 
     3061 TGACTTGGAA CTGTGTGATG AGGCCTTGGC CCTCCTGGAT GAGGTCATCA TGTGTACTTT 
     3121 CCAGCAGTCG GTCTACTACC TCACCAAGAC TCTTTATTCA ACACTGCCAG CTCTCCTGGA 
     3181 CAGTAACCCT TTCACAGCTG GAGCAGAGCT GCCAGGGCCT GGCGCAGAGC TGGAGGCCAT 
     3241 GCCGCCTGGG CTCAGGCCTA CTCTGGGTGT GTTCCAGGCA GCTCTAGAAC TGACCAGTCA 
     3301 GTGTGAGCTG CATCCCGACC TCGTGTCCCA GACTTTTGGC TATTTGTTCT TCTTCTCCAA 
     3361 TGCATCCCTT CTCAACTCAC TGATGGAACG AGGTCAAGGC CGACCTTTCT ATCAATGGTC 
     3421 TCGAGCCGTC CAAATCAGAA CCAACTTGGA TCTTGTGTTG GATTGGCTTC AGGGGGCCGG 
     3481 GCTGGGTGAC ATTGCCACTG AGTTCTTCCG GAAACTTTCC ATTGCTGTGA ACCTGCTATG 
     3541 TGTGCCCCGC ACCTCCCTGC TCAAGGCTTC ATGGAGCAGC TTACGGACTG ACTACCCCAC 
     3601 CCTGACTCCA GCTCAGCTCC ACCATCTCCT CAGCCACTAC CAGTTGGGTC CTGGCCGTGG 
     3661 TCCCCCACCA GCCTGGGACC CACCACCTGC AGAGCGGGAT GCTGTGGACA CAGGGGATAT 
     3721 CTTTGAAAGT TTTTCCTCGC ACCCTCCGCT CATCCTGCCC CTGGGCAGCT CACGCCTGCG 
     3781 CCTCACCGGC CCTGTGACAG ATGACGCATT GCATAGAGAA CTGCGCAGGC TCCGTCGCCT 
     3841 CCTCTGGGAT CTTGAACAGC AGGAGCTGCC AGCCAATCAT CGCCATGGAC CCCCGGTGGC 
     3901 TTCGACACCT AAGCTTCACT AGTTAGCGGA CCGACGCGTA CGCGGCCGCT CGAGATGGAG 
     3961 AGCGACGAGA GCGGCCTGCC CGCCATGGAG ATCGAGTGCC GCATCACCGG CACCCTGAAC 
     4021 GGCGTGGAGT TCGAGCTGGT GGGCGGCGGA GAGGGCACCC CCGAGCAGGG CCGCATGACC 
     4081 AACAAGATGA AGAGCACCAA AGGCGCCCTG ACCTTCAGCC CCTACCTGCT GAGCCACGTG 
     4141 ATGGGCTACG GCTTCTACCA CTTCGGCACC TACCCCAGCG GCTACGAGAA CCCCTTCCTG 
     4201 CACGCCATCA ACAACGGCGG CTACACCAAC ACCCGCATCG AGAAGTACGA GGACGGCGGC 
     4261 GTGCTGCACG TGAGCTTCAG CTACCGCTAC GAGGCCGGCC GCGTGATCGG CGACTTCAAG 
     4321 GTGATGGGCA CCGGCTTCCC CGAGGACAGC GTGATCTTCA CCGACAAGAT CATCCGCAGC 
     4381 AACGCCACCG TGGAGCACCT GCACCCCATG GGCGATAACG ATCTGGATGG CAGCTTCACC 
     4441 CGCACCTTCA GCCTGCGCGA CGGCGGCTAC TACAGCTCCG TGGTGGACAG CCACATGCAC 
     4501 TTCAAGAGCG CCATCCACCC CAGCATCCTG CAGAACGGGG GCCCCATGTT CGCCTTCCGC 
     4561 CGCGTGGAGG AGGATCACAG CAACACCGAG CTGGGCATCG TGGAGTACCA GCACGCCTTC 
     4621 AAGACCCCGG ATGCAGATGC CGGTGAAGAA AGAGTTTAAA CGGCCGGCCG CGGTCATAGC 
     4681 TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC 
     4741 CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT 
     4801 TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA 
     4861 GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG 
     4921 TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT 
     4981 GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT 
     5041 GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC 
     5101 TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC 
     5161 TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA 
     5221 GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT 
     5281 CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCTTG 
     5341 GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG 
     5401 TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC 
     5461 AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT 
     5521 AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG 
     5581 ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA 
     5641 GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT 
     5701 ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA 
     5761 TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC 
     5821 ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC 
     5881 GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC 
     5941 GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC 
     6001 TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC 
     6061 TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC 
     6121 GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG 
     6181 TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT 
     6241 CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT 
     6301 CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG 
     6361 ATTCATCGAC TGTGGCCGGC TGGGTGTGGC CGACCGCTAT CAGGACATAG CGTTGGCTAC 
     6421 CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG 
     6481 TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG 
     6541 AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT 
     6601 TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC 
     6661 GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG 
     6721 TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA 
     6781 GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT 
     6841 GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT 
     6901 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT 
     6961 AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC 
     7021 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG 
     7081 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG 
     7141 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA 
     7201 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC 
     7261 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC 
     7321 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG 
     7381 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC 
     7441 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT 
     7501 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG 
     7561 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC 
     7621 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT 
     7681 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT 
     7741 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC 
     7801 AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA 
     7861 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA 
     7921 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT 
     7981 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC 
     8041 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC 
     8101 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC 
     8161 CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA 
     8221 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC 
     8281 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC 
     8341 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA 
     8401 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA 
     8461 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA 
     8521 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT 
     8581 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT 
     8641 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG 
     8701 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA 
     8761 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC 
     8821 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG 
     8881 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8941 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     9001 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     9061 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     9121 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     9181 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     9241 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     9301 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     9361 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     9421 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//