LOCUS       MG216571                9260 bp    DNA     circular  SYN 
DEFINITION  Zfp574 (tGFP-tagged) - Mouse zinc finger protein 574 (Zfp574) transcript variant 1, (10ug)
ACCESSION   NM_175477
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3741..4445 
                     /label="GFP"  
     misc_feature    4492..5077 
                     /label="PolyA signal"  
     misc_feature    7169..7814 
                     /label="ColE1"  
     misc_feature    5531..6325 
                     /label="Neo"  
     misc_feature    5162..5464 
                     /label="SV40 ori"  
     misc_feature    7829..8689 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3720..3726 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3720 
                     /label="Zfp574"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTGAGGAG AGTGAAGAGA CGGTCCTGTA CATTGAGCAC CGCTATGTCT 
     1081 GCTCTGAGTG CAACCAGCTC TATGGATCCT TGGAGGAGGT GCTTGTGCAC CAGAACTCCC 
     1141 ACGTGCCCCA GCAGCACTTT GAGCTGGTGG GCGTGGCTGA CCCTGGAGTC ACAGTGGCCA 
     1201 CAGAGGCTGC TTCAGGCACA GGCCTATATC AGACCCTCAT CCAAGAGAGC CAGTACCAGT 
     1261 GTCTTGAGTG TGGGCAACTG CTCCTGTCCC CCAGCCAGCT CCTGGAGCAC CAGGAGCTAC 
     1321 ACCTGAAGAT GATGGCACCG CAGGAGGCAG TGCCAGCCAA GCCACCACCC AAGGTGCCCC 
     1381 CCTTGGGCTC CAGCGCCATC CACTATGAGT GTGTGGACTG TAAGGCTCTG TTCGCCAGCC 
     1441 AAGAGATGTG GCTTAGCCAT CGCCAGACGC ATCTCAGAGC CACGCCCAAC AAGGCTCCTG 
     1501 CTCCTGTCGT CTTGGGGTCC CCAGTTGTTT TGGGCCCTCC TGTGGGCCAG GCTCGAGTGG 
     1561 CTGTGGAACA TTCATACCGA AAAGCAGAAG AGGGTGGGGA GGGGGCGGCC GTTCCGTCTG 
     1621 TGGCCGCCAC CACCGAGATG GTGACAGAAG TGGAGCTGCT CCTCTACAAG TGCTCCGAGT 
     1681 GCTCCCAGCT CTTCCAGATG CCCGCTGACT TCCTGGAGCA CCAGGCCACC CACTTCCCTG 
     1741 CTCCTGTCCC GGAGGCTGCG GAGCCTGCCA CACAGCAGGA AACCCAGGTC CCCTCACCCA 
     1801 CTGAGGCAGC AGTGTCTCAG CCCGAACCCC TGCCAGCCTC TGATCACAGT TATGAGCTGC 
     1861 GGAATGAGCT GCGAAATGGG GAGGCCATTG GGCGAGACCG CAGGGGACGG AAGCCAAGGA 
     1921 GGAACAACAG TGGAGAGTCA GGTGGGGCGG CCACCCAGGA ACTATTTTGC TCTGCCTGTG 
     1981 ACCAGCTCTT TCTCTCACCC CACCAGCTAC AGCAGCACCT TCGGAGTCAC CGGGAGGGTG 
     2041 TTTTTAAGTG TCCCCTGTGC AGTCGTGTCT TCCCTAGCCC TTCTAGTCTG GACCAGCACC 
     2101 TTGGTGACCA TAGCAGTGAG TCTCACTTCC TGTGTGTAGA CTGTGGCCTA GCCTTTGGCA 
     2161 CCGAAGCCCT CCTCCTGGCC CACCGGCGAG CCCATACCCC GAATCCTCTG CATTCGTGTC 
     2221 CCTGTGGGAA GACCTTTGTT AACCTCACCA AGTTCCTTTA TCACCGGCGT ACCCACGGAG 
     2281 CAGGAGGTGT CCCTTTGCCC ACAACACCAG TTCCACCAGA GGAGCCTGCT ATTAGCTTTC 
     2341 CTGAGCCAGC CCCTGCAGAG ACCGGAGAGC TAGAGGCCCC AGAGCTCCCC GTATGTGAGG 
     2401 AGAGTTCAGC CGAGCCTGCA GCTCCAGGCT CTTACCGCTG CCTCCTGTGT AGCCGGGAGT 
     2461 TTGGTAAGGC GTTGCAGCTG ACCCGGCACC AGCGTTTTGT GCACCGGCTA GAGCGGCGCC 
     2521 ATAAGTGCAG CATTTGTGGC AAGATGTTCA AGAAGAAGTC TCATGTGCGG AACCATCTGC 
     2581 GTACACACAC TGGGGAGCGG CCCTTCCCCT GCCCTGACTG CTCTAAGCCT TTCAACTCAC 
     2641 CAGCCAACCT GGCCCGCCAC CGGCTCACAC ACACGGGGGA GCGGCCCTAC CGGTGTGGGG 
     2701 ACTGCGGCAA GGCCTTCACG CAGAGCTCCA CTCTGAGGCA GCATCGCCTG GTGCATGCCC 
     2761 AGCACTTCCC CTACCGCTGC CAGGAGTGCG GAGTCCGTTT TCACCGCCCT TATCGCCTGC 
     2821 TCATGCACCG CTACCACCAC ACAGGCGAGT ACCCCTACAA GTGCCGTGAG TGCCCCCGTT 
     2881 CCTTCTTGCT ACGCCGGCTG CTAGAGGTCC ACCAGCTTGT GATCCATGCT GGGCGCCAGC 
     2941 CCTACCGCTG CTCATCCTGT GGGGCCGCCT TCCCCTCGTC ACTGCGGCTC CGTGAGCATC 
     3001 GCTGCGCGGC CGCTGCTGCC CAGGCCCCAC GGCGCTTTGA GTGTGGGACC TGTGGCAAGA 
     3061 AAGTGGGCTC TGCTGCTCGC CTGCAGGCAC ATGAAGCCGC CCATGCTGCT GCGGGTCCTG 
     3121 GAGAGGTCTT AGCCAAGGAG CCTCCTGCTC CCCGGGCCTC GAGAGCCACT CGCACACCAG 
     3181 TCGCCCCCTC CCCGACAGCC CTCAGTGGTA CAACCTCTGC TGCCCCTGCA GCCCCTGCCC 
     3241 GGCGTCGGGG TCCGGAGTGC AGTGAGTGCA AGAAGCTCTT CAGCACAGAG ACGTCACTAC 
     3301 AGGTCCATCG GCGCATCCAC ACAGGTGAGC GGCCATACCC CTGTCCAGAC TGTGGCAAGG 
     3361 CCTTCCGCCA GAGTACCCAT CTGAAAGACC ACAGACGCCT ACACACAGGC GAGCGGCCCT 
     3421 TTGCGTGTGA AGTGTGTGGC AAGGCCTTTG CCATCTCCAT GCGTCTGGCA GAACATCGCC 
     3481 GCATCCACAC GGGTGAGCGA CCCTACTCCT GTCCTGACTG TGGCAAGAGC TACCGTTCCT 
     3541 TCTCCAATCT CTGGAAGCAC CGCAAGACCC ACCAGCAGCA ACACCAGGCG GCCGTCCGAC 
     3601 AGCAGCTGGC AGAGGCGGAG GCTGCTGTGG GCCTGGCTGT GATGGAAACT GCAGTGGAGG 
     3661 CTCTGCCCCT TGTGGAGGCC ATTGAGATCT ACCCTCTAGC TGAAGCTGAT GGGGTCCAGA 
     3721 TCAGTGGCAC GCGTACGCGG CCGCTCGAGA TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA 
     3781 TGGAGATCGA GTGCCGCATC ACCGGCACCC TGAACGGCGT GGAGTTCGAG CTGGTGGGCG 
     3841 GCGGAGAGGG CACCCCCGAG CAGGGCCGCA TGACCAACAA GATGAAGAGC ACCAAAGGCG 
     3901 CCCTGACCTT CAGCCCCTAC CTGCTGAGCC ACGTGATGGG CTACGGCTTC TACCACTTCG 
     3961 GCACCTACCC CAGCGGCTAC GAGAACCCCT TCCTGCACGC CATCAACAAC GGCGGCTACA 
     4021 CCAACACCCG CATCGAGAAG TACGAGGACG GCGGCGTGCT GCACGTGAGC TTCAGCTACC 
     4081 GCTACGAGGC CGGCCGCGTG ATCGGCGACT TCAAGGTGAT GGGCACCGGC TTCCCCGAGG 
     4141 ACAGCGTGAT CTTCACCGAC AAGATCATCC GCAGCAACGC CACCGTGGAG CACCTGCACC 
     4201 CCATGGGCGA TAACGATCTG GATGGCAGCT TCACCCGCAC CTTCAGCCTG CGCGACGGCG 
     4261 GCTACTACAG CTCCGTGGTG GACAGCCACA TGCACTTCAA GAGCGCCATC CACCCCAGCA 
     4321 TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT TCCGCCGCGT GGAGGAGGAT CACAGCAACA 
     4381 CCGAGCTGGG CATCGTGGAG TACCAGCACG CCTTCAAGAC CCCGGATGCA GATGCCGGTG 
     4441 AAGAAAGAGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4501 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4561 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4621 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4681 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4741 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4801 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4861 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4921 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4981 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5041 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5101 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCTTGGCTGT GGAATGTGTG TCAGTTAGGG 
     5161 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5221 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5281 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 
     5341 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 
     5401 GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 
     5461 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 
     5521 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 
     5581 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 
     5641 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 
     5701 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 
     5761 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 
     5821 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 
     5881 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 
     5941 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 
     6001 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 
     6061 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 
     6121 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 
     6181 GTGGCCGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 
     6241 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 
     6301 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA 
     6361 CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG 
     6421 AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG 
     6481 ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 
     6541 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 
     6601 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT 
     6661 AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA 
     6721 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 
     6781 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 
     6841 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT 
     6901 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 
     6961 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 
     7021 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 
     7081 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 
     7141 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 
     7201 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 
     7261 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 
     7321 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 
     7381 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 
     7441 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 
     7501 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 
     7561 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT 
     7621 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 
     7681 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 
     7741 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 
     7801 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 
     7861 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 
     7921 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 
     7981 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 
     8041 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 
     8101 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 
     8161 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 
     8221 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 
     8281 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 
     8341 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 
     8401 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 
     8461 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 
     8521 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 
     8581 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 
     8641 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 
     8701 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 
     8761 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 
     8821 TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA 
     8881 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT 
     8941 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT 
     9001 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC 
     9061 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT 
     9121 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT 
     9181 TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC 
     9241 AAAAATTTAA CGCGAATTTT 
//