LOCUS MG216498 7907 bp DNA circular SYN DEFINITION Gpr61 (tGFP-tagged) - Mouse G protein-coupled receptor 61 (Gpr61), (10ug) ACCESSION NM_175470 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 2388..3092 /label="GFP" misc_feature 3139..3724 /label="PolyA signal" misc_feature 5816..6461 /label="ColE1" misc_feature 4178..4972 /label="Neo" misc_feature 3809..4111 /label="SV40 ori" misc_feature 6476..7336 /label="Amp" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 2367..2373 /label="Restriction Enzyme" misc_feature 1020..2367 /label="Gpr61" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGAGTCCTCA CCCATCCCCC AGTCATCAGG AAACTCGTCC ACTTTGGGAA 1081 GGGCCCTTCA AACCCCAGGT CCCTCTACTG CCAGCGGGGT CCCAGAGTTG GGATTACGGG 1141 ACGTGGCTTC AGAATCTGTG GCCCTCTTCT TCATGCTCCT GTTGGATCTC ACTGCTGTGG 1201 CTGGCAATGC TGCTGTGATG GCTGTTATTG CCAAGACACC CGCCCTCCGA AAATTTGTTT 1261 TTGTCTTCCA TCTTTGTCTG GTGGACCTGC TGGCTGCCCT GACCCTCATG CCGCTTGCCA 1321 TGCTCTCCAG CTCTGCCCTC TTTGACCACG CCCTCTTTGG GGAGGTGGCC TGCCGCCTCT 1381 ACCTGTTCCT GAGCGTTTGC TTTGTCAGCC TGGCCATCCT TTCGGTGTCT GCCATTAATG 1441 TGGAGCGCTA CTATTATGTG GTCCACCCAA TGCGCTACGA GGTGCGCATG ACACTAGGGC 1501 TGGTGGCCTC CGTGCTGGTG GGCGTGTGGG TAAAGGCCCT AGCCATGGCT TCTGTGCCAG 1561 TGTTGGGAAG GGTCTACTGG GAGGAAGGAG CTCCCAGTGT TAACCCCGGC TGTTCTCTCC 1621 AATGGAGCCA TAGTGCCTAC TGCCAGCTTT TTGTGGTGGT CTTTGCTGTT CTGTACTTCT 1681 TGCTGCCCTT GATCCTGATC TTTGTGGTCT ACTGCAGCAT GTTTCGAGTG GCTCGCGTGG 1741 CTGCCATGCA ACACGGGCCG CTGCCCACGT GGATGGAGAC GCCCCGGCAA CGCTCTGAGT 1801 CTCTCAGTAG CCGCTCTACT ATGGTTACCA GCTCCGGGGC TCACCAGACC ACCCCACACC 1861 GGACGTTTGG GGGTGGGAAG GCAGCAGTGG TCCTCCTGGC TGTAGGGGGA CAGTTCTTGC 1921 TTTGTTGGTT GCCCTACTTC TCTTTCCATC TCTATGTTGC CCTGAGCGCA CAGCCCATTT 1981 CAGCAGGACA GGTGGAGAAC GTGGTAACCT GGATTGGCTA CTTTTGCTTC ACTTCCAACC 2041 CCTTTTTCTA CGGATGTCTC AACCGTCAGA TCCGGGGCGA GCTTAGCAAA CAGTTTGTCT 2101 GCTTCTTCAA GGCAGCTCCA GAGGAGGAGC TGAGGCTGCC TAGTCGTGAG GGCTCCATTG 2161 AGGAGAATTT CCTGCAGTTC CTCCAGGGGA CCTCTGAGAA CTGGGTTTCT CGGCCCCTAC 2221 CCAGTCCTAA GCGGGAGCCA CCCCCTGTTG TTGACTTTCG AATCCCAGGC CAGATTGCTG 2281 AGGAGACCTC AGAGTTCCTG GAGCAGCAAC TCACCAGCGA CATCATCATG TCCGACAGCT 2341 ACCTCCGTCC CGCCCCCTCA CCAAGGCTGG AGTCAACGCG TACGCGGCCG CTCGAGATGG 2401 AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG CCGCATCACC GGCACCCTGA 2461 ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC CCCCGAGCAG GGCCGCATGA 2521 CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG CCCCTACCTG CTGAGCCACG 2581 TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG CGGCTACGAG AACCCCTTCC 2641 TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT CGAGAAGTAC GAGGACGGCG 2701 GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG CCGCGTGATC GGCGACTTCA 2761 AGGTGATGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT CACCGACAAG ATCATCCGCA 2821 GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA CGATCTGGAT GGCAGCTTCA 2881 CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTC CGTGGTGGAC AGCCACATGC 2941 ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG GGGCCCCATG TTCGCCTTCC 3001 GCCGCGTGGA GGAGGATCAC AGCAACACCG AGCTGGGCAT CGTGGAGTAC CAGCACGCCT 3061 TCAAGACCCC GGATGCAGAT GCCGGTGAAG AAAGAGTTTA AACGGCCGGC CGCGGTCATA 3121 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 3181 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 3241 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 3301 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 3361 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 3421 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 3481 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 3541 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 3601 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 3661 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 3721 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCT 3781 TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 3841 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 3901 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 3961 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 4021 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 4081 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 4141 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 4201 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 4261 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 4321 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 4381 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 4441 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 4501 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 4561 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 4621 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 4681 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 4741 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 4801 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCCGACCGCT ATCAGGACAT AGCGTTGGCT 4861 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 4921 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 4981 TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 5041 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 5101 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 5161 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 5221 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 5281 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 5341 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 5401 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 5461 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 5521 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 5581 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 5641 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 5701 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 5761 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 5821 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 5881 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 5941 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 6001 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 6061 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 6121 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 6181 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 6241 GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 6301 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 6361 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 6421 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 6481 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 6541 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 6601 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 6661 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 6721 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 6781 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 6841 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 6901 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 6961 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 7021 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 7081 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 7141 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 7201 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 7261 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 7321 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 7381 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 7441 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA 7501 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 7561 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 7621 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 7681 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 7741 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 7801 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 7861 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT //