LOCUS       MG216306                9512 bp    DNA     circular  SYN 
DEFINITION  Nek9 (tGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9), (10ug)
ACCESSION   NM_145138
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3993..4697 
                     /label="GFP"  
     misc_feature    4744..5329 
                     /label="PolyA signal"  
     misc_feature    7421..8066 
                     /label="ColE1"  
     misc_feature    5783..6577 
                     /label="Neo"  
     misc_feature    5414..5716 
                     /label="SV40 ori"  
     misc_feature    8081..8941 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3972..3978 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3972 
                     /label="Nek9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGTGCTG GGCGAGTACG AGCGACACTG CGATTCCATC AACTCGGACT 
     1081 TCGGAAGCGA GTCCGGGGGT GGCGGGGACT CGGGCCCGGG CCCCAGCGCT GTCCCAGGGC 
     1141 CGCGAGCCGG CGGCGGGGCG GCGGAGCAGG AGGAGCTGCA CTACATCCCC ATCCGCGTTC 
     1201 TGGGCCGCGG TGCCTTTGGG GAGGCCACGC TGTACCGCCG CACCGAGGAC GACTCACTGG 
     1261 TGGTGTGGAA GGAAGTTGAC TTGACCCGGC TATCTGAGAA GGAACGTCGA GATGCCCTGA 
     1321 ATGAGATTGT CATCCTGGCA CTGCTACAGC ACGACAACAT CATTGCCTAC TACAACCACT 
     1381 TCATGGACAA CACCACCCTG CTGATTGAGC TGGAGTACTG TAACGGAGGG AACCTCTATG 
     1441 ACAAAATCCT TCGTCAGAAG GACAAGTTGT TCGAAGAAGA GATGGTGGTG TGGTACCTTT 
     1501 TTCAGATTGT TTCAGCAGTG AGCTGTATTC ATAAAGCTGG AATCCTCCAT AGAGATATAA 
     1561 AGACATTAAA TATTTTTCTG ACCAAGGCAA ATCTGATAAA GCTTGGAGAC TATGGCCTAG 
     1621 CAAAGAAACT TAATTCTGAG TACTCTATGG CCGAGACACT TGTGGGCACT CCATATTACA 
     1681 TGTCTCCAGA GCTCTGCCAA GGAGTAAAAT ACAATTTCAA GTCTGATATA TGGGCAGTTG 
     1741 GCTGTGTCAT TTTTGAACTG CTTACTCTGA AAAGAACATT TGATGCTACA AACCCACTCA 
     1801 ACCTATGTGT GAAGATCGTG CAGGGAATCC GGGCCATGGA AGTTGACTCG AGTCAGTATT 
     1861 CTTTGGAACT GATCCAGTTG GTACACGCAT GCCTCGACCA GGATCCTGAG CAGAGGCCCG 
     1921 CTGCAGATGC ACTCCTGGAT CTCCCCCTTC TCAGGACCCG CAGGAGAGAG ATGGAGGAAA 
     1981 AAGTCACTCT GCTCAATGCA CCTACAAAGA GACCAAGGTC AAGCACTGTG ACGGAAGCAC 
     2041 CCATTGCTGT GGTAACATCA CGAACCAGTG AAGTCTATGT TTGGGGTGGT GGAAAATCCA 
     2101 CACCTCAGAA ATTGGACGTC ATCAAGAGTG GCTGTAGTGC TCGACAGGTG TGTGCGGGGA 
     2161 ACACCCACTT TGCTGTGGTC ACCGTGGAGA AGGAACTATA TACCTGGGTG AACATGCAAG 
     2221 GGGGCACTAA ACTTCACGGT CAGCTAGGAC ATGGAGACAA AGCTTCCTAC CGACAGCCAA 
     2281 AGCATGTAGA AAAGTTACAA GGGAAGGCTA TCCACCAGGT GTCGTGTGGG GATGATTTCA 
     2341 CTGTCTGTGT CACAGATGAG GGTCAGCTCT ACGCCTTTGG ATCAGATTAT TATGGCTGCA 
     2401 TGGGAGTGGA CAAGGTTTCT GGCCCTGAAG TGCTAGAACC CATGCAGCTG AACTTCTTCC 
     2461 TCAGCAATCC TGTGGAGCAG GTCTCCTGTG GGGATAATCA TGTGGTAGTT CTGACACGAA 
     2521 ACAAAGAAGT CTACTCTTGG GGCTGTGGTG AATATGGACG GCTGGGTTTG GATTCAGAAG 
     2581 AGGATTACTA TACACCACAG AGGGTGGATG TTCCAAAGGC CTTAATCATT GTTGCAGTTC 
     2641 AATGTGGCTG TGATGGGACC TTTCTGCTCA CTCAGTCAGG CAAAGTGCTG GCCTGTGGAC 
     2701 TCAATGAGTT CAACAAGCTG GGGCTGAATC AGTGCATGTC TGGAATCATC AACCATGAAG 
     2761 CATACCATGA AGTTCCCTAC ACAACCTCCT TTACCTTGGC CAAACAGTTG TCCTTTTACA 
     2821 AAATCCGTAC CATTGCCCCA GGCAAGACTC ACACAGCTGC TATTGATGAA CGAGGCCGGT 
     2881 TGCTGACTTT TGGCTGTAAT AAGTGTGGGC AGCTGGGTGT TGGGAATTAT AAGAAACGCC 
     2941 TGGGAATCAA CCTGTTGGGG GGACCCCTTG GCGGGAAGCA AGTGATTAGG GTCTCGTGTG 
     3001 GCGATGAGTT CACCATTGCT GCCACCGACG ATAATCACAT CTTTGCCTGG GGTAATGGTG 
     3061 GTAATGGCCG CCTGGCAATG ACTCCCACAG AGAGACCACA TGGCTCTGAT ATCTGTACCT 
     3121 CATGGCCTCG GCCTATTTTT GGATCCCTGC ATCATGTTCC AGACCTTTCT TGCCGTGGCT 
     3181 GGCACACCAT TCTTATTGTT GAGAAAGTGT TGAATTCCAA GACCATCCGT TCCAATAGCA 
     3241 GTGGCCTGTC CATCGGAACT GTGGTTCAGA GCTCCAGCCC AGGAGGTGGC ATTGGTGGTG 
     3301 GTGGTGGTGG TGGCGGCGGC GGCGGGGGTG AAGAGGAAGA CAGTCAACAG GAATCTGAAA 
     3361 CTCCAGATCC AAGCGGAGGC TTCCGAGGAA CAATGGAAGC AGACCGTGGG ATGGAAGGCT 
     3421 TAATCAGTCC CACGGAGGCC GTGGGAAACA GCTGTGGGGC TAGCAGCTCC TGTCCTGGCT 
     3481 GGCTTCGGAA GGAGCTGGAA AACGCAGAGT TCATTCCCAT GCCTGACAGC CCCGCTCCCT 
     3541 TAAGTGCGGC GTTTTCACAG TCTGAGAAGG ACACGCTGCC CTATGAAGAG CTGCAGGGAC 
     3601 TCAAGGTCGC ATCTGAAGTT CCTCCGGAGC CCCAGCGTGC AGCAGGAGCC TGGCCCCCTC 
     3661 GGCTGGATCC TGCAGTGCCG TGTGTTGGGA AAGCCCTGAC CTCTGCTGCT TGTGCGTGCA 
     3721 GTGCTCTGCA GGTGGAGGTT GACAGGTTGC AGGCTCTGGT GTTAAAGTGT CTGGAAGAAC 
     3781 AGCAGAAGCT CCAGCAAGAA AACCTCCAGA TGTTTACCCA ACTACAAAAG CTCAACAAGA 
     3841 AATTGGAAGG AGGCCAGCAG GTGGGGATGC ACTCCAGAGG AACCCAGACA GCAAAGGAAG 
     3901 AAATGGAAAT GGATCCAAAG CCTGACTTAG ACTCGGAGTC CTGGTGCCTC CTCGGAACAG 
     3961 ACTCTTGTCG ACCCAGCCTC ACGCGTACGC GGCCGCTCGA GATGGAGAGC GACGAGAGCG 
     4021 GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG 
     4081 AGCTGGTGGG CGGCGGAGAG GGCACCCCCG AGCAGGGCCG CATGACCAAC AAGATGAAGA 
     4141 GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT 
     4201 TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA 
     4261 ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA 
     4321 GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG ATGGGCACCG 
     4381 GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG 
     4441 AGCACCTGCA CCCCATGGGC GATAACGATC TGGATGGCAG CTTCACCCGC ACCTTCAGCC 
     4501 TGCGCGACGG CGGCTACTAC AGCTCCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA 
     4561 TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGG 
     4621 ATCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCGGATG 
     4681 CAGATGCCGG TGAAGAAAGA GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4741 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4801 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4861 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4921 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4981 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5041 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5101 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5161 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5221 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5281 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5341 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCTTGGCT GTGGAATGTG 
     5401 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5461 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5521 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 
     5581 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 
     5641 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 
     5701 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 
     5761 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 
     5821 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 
     5881 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 
     5941 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 
     6001 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 
     6061 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 
     6121 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 
     6181 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 
     6241 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 
     6301 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 
     6361 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 
     6421 GGCCGGCTGG GTGTGGCCGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 
     6481 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 
     6541 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 
     6601 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 
     6661 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 
     6721 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 
     6781 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 
     6841 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 
     6901 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
     6961 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 
     7021 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
     7081 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
     7141 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 
     7201 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     7261 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     7321 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     7381 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     7441 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     7501 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     7561 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     7621 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     7681 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     7741 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     7801 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     7861 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7921 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     7981 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 
     8041 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 
     8101 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 
     8161 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 
     8221 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 
     8281 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 
     8341 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 
     8401 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 
     8461 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 
     8521 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 
     8581 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 
     8641 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 
     8701 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 
     8761 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 
     8821 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 
     8881 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 
     8941 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     9001 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     9061 TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 
     9121 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 
     9181 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
     9241 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 
     9301 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 
     9361 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 
     9421 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 
     9481 AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//