LOCUS       MG216112                9524 bp    DNA     circular  SYN 
DEFINITION  Eml4 (tGFP-tagged) - Mouse echinoderm microtubule associated protein like 4 (Eml4) transcript variant 1, (10ug)
ACCESSION   NM_001114361
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4005..4709 
                     /label="GFP"  
     misc_feature    4756..5341 
                     /label="PolyA signal"  
     misc_feature    7433..8078 
                     /label="ColE1"  
     misc_feature    5795..6589 
                     /label="Neo"  
     misc_feature    5426..5728 
                     /label="SV40 ori"  
     misc_feature    8093..8953 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3984..3990 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3984 
                     /label="Eml4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACGGTTTC GCCGGCAGCC TCGATGATAG TATTTCTGCT GCAAGTACCT 
     1081 CTGACGTTCA AGACCGCCTC TCAGCTCTTG AGTCACGAGT TCAACAACAA GAAGATGAAA 
     1141 TCACTGTGCT AAAGGCAGCA TTGGCTGATG TTTTGAGGCG TCTTGCAATC TCTGAAGATC 
     1201 ATGTAGCCTC AGTGAAAAAG TCCATGCCAA GTAAAGGCCA ACCAAGCCTT CGAGAAGCTA 
     1261 TTTCTATGTC CTGTATAACC AATGGAAGTG GTATAAGCAG AAAACAGAAT CATACCAGCT 
     1321 CTGTATCAAT AGCAAGAAAA GAAACTCTTT CTTCAGCTGC TAAAAGTGGT ACAGAAAAAA 
     1381 AGAAAGAAAA GCCACAAGGA CAGAGAGAAA AAAAAGAGGA TTCTCATTCT AATGATCAGA 
     1441 GCCCACAAAT TCGAGCGTCA CCTTCTCCCC AGCCCTCTTC ACAGCCTCTC CAAATAAACA 
     1501 GACAAACTCC AGAGAGCAAG AGTTCTGCTC CCATCAAAAG CATAAAACGT CCACCAACAG 
     1561 CTGAAAAGTC ACATAACTCA TGGGAAAATT CAGATGATAG TCGTAATAAA TTAATGAAAA 
     1621 CGGTTTCAAC ATCAAAACTA ATATCAAAGG TTATAAAAAA TGCAGACAAA CATAAAGATG 
     1681 TCATCGTCAA TCAAGCAAAA ATGTCAACCC GGGAAAAAAA CAGCCAAGAA GGAGAATATA 
     1741 TTAAAATGTT TATGCGAGGT CGGCCAATTA CAATGTTCAT TCCTTCGGAT GTTGACAACT 
     1801 ATGATGACAT CAGAACAGAA CTGCCTCCAG AGAAGCTCAA ACTGGAGTGG GTATACGGTT 
     1861 ATCGAGGAAA GGACTGTAGA GCGAATGTAT ACCTGCTTCC CACAGGGGAG ATAGTTTATT 
     1921 TTATCGCCTC AGTGGTAGTC CTGTTTAATT ATGAGGAGAG GACTCAGCGA CACTACCTGG 
     1981 GTCATACAGA CTGTGTGAGA TGCCTTGCTG TACATCCTGA CAAGATTAGA ATTGCAACTG 
     2041 GACAGATAGC CGGAGTAGAC AAAGATGGAA GGCCTCTGCA ACCCCATGTC AGGGTGTGGG 
     2101 ATTCAGTAAG TCTTACCACA CTGCATGTTA TTGGACTTGG AACTTTTGAG CGTGGAGTAG 
     2161 GATGCCTGGA TTTTTCAAAA GCAGATTCAG GTGTTCATTT ATGCGTTATT GATGACTCCA 
     2221 ATGAGCACAT GCTTACTGTA TGGGACTGGC AGAAAAAATC AAAAATCGCA GAAATAAAGA 
     2281 CAACAAACGA AGTTGTTTTG GCTGTAGAAT TCCACCCAAC AGATGCAAAT ACAATAATAA 
     2341 CATGTGGTAA ATCTCATATT TTTTTCTGGA CCTGGAGTGG CAACTCACTA ACAAGAAAAC 
     2401 AGGGAATTTT TGGGAAATAT GAAAAACCAA AATTCGTTCA GTGTTTGGCA TTCTTGGGGA 
     2461 ATGGAGATGT TCTCACTGGA GACTCGGGTG GAGTCATGCT GATCTGGAGC AAAACGATGG 
     2521 TAGAGCCCCC GCCCGGGAAA GGACCTAAAG GTGTGTATCA GATCAACAGA CAAATCAAAG 
     2581 CTCATGATGG CAGTGTGTTT ACACTTTGTC AGATGAGAAA TGGGATGCTA TTAACTGGAG 
     2641 GCGGAAAAGA CAGAAAAATA ATTTTGTGGG ATCATGATCT GAACCTTGAA AGAGAAATAG 
     2701 AGGTTCCTGA TCAGTATGGC ACCATTAGAG CAGTTGCAGA GGGAAGGGCA GAACAGTTCT 
     2761 TAGTAGGCAC ATCACGAAAC TTTATTTTAC GGGGAACGTT TAATGATGGC TTCCAAATAG 
     2821 AAGTCCAGGG TCATACAGAT GAACTCTGGG GCCTTGCAAC ACATCCCTTC AAAGATTTGC 
     2881 TCTTGACGTG TGCTCAGGAC AGGCAGGTGT GCATGTGGAA CTCAGTGGAG CACAGGCTGG 
     2941 AGTGGACCAG GCTTGTGGAC GAACCAGGAC ACTGTGCAGA CTTTCATCCA AGCGGTACAG 
     3001 TGGTAGCCAT TGGAACGCAC TCAGGCAGGT GGTTTGTTCT GGATGCAGAA ACAAGAGATC 
     3061 TGGTGTCAAT CCACACGGAT GGCAATGAGC AGCTGTCTGT GATGCGCTAC TCAGTAGATG 
     3121 GCACTCTTCT GGCTGTGGGC TCTCATGACA ACTTTATTTA CCTCTACACA GTCTTAGAAA 
     3181 ACGGAAGAAA ATACAGCAGA TATGGAAAGT GCACTGGACA TTCTAGCTAC ATCACACACC 
     3241 TTGACTGGTC CCCAGACAAT AAGCATATAA TGTCTAACTC GGGTGACTAC GAGATACTGT 
     3301 ACTGGGACAT TGAAAATGGC TGCAAACTAA TCAGGAACCG ATCAGATTGC AAGGACATCG 
     3361 ATTGGACAAC ATATACCTGT GTGCTAGGCT TCCAAGTCTT TGGGGTGTGG CCGGAAGGGT 
     3421 CCGATGGAAC AGACATCAAC GCACTGGTTC GATCCCACAA CAGAAGAGTG ATCGCAGTGG 
     3481 CCGATGATTT CTGCAAAGTT CATCTCTTTC AGTACCCCTG CTCCAAAGCC AAGGCTCCCA 
     3541 GTCACAAGTA CAGTGCCCAC AGCAGCCATG TGACCAACGT CAGTTTTACT CATAATGACA 
     3601 GTCACCTAAT TTCAACTGGT GGGAAGGATA TGAGCATCAT TCAGTGGAAG CTTGTGGAAA 
     3661 AGTTACCTGT GCCTCAAAAC GAGGTCATAA CAGACGCCAG TGTGACCAAA ACCCCTGCCT 
     3721 CTTCCTCTGA AACTGCCAGG CCGTCTAACT CTCCTCCACT GCCTCCTTCT CTACCGTTAA 
     3781 CTGGGACGGC TGAAGAGGAA AGCAGGATGG GCAGCTCCCC CACACTAGTG GAGAACAGCC 
     3841 TGGAGCAGAT CGCAGAGCCC AGCGAGGAGC AGAGCGAGTG GGGTAGCGAA GACCTTGGTG 
     3901 TGGTGATTGA TGAAGAGCCT GCTAGTGAGT TAAGTGAGAC ACAGGGAGCA ACTGAGCTGC 
     3961 CTGAGGAGGA GCGAGGCATA ACACCCCTGT GCACGCGTAC GCGGCCGCTC GAGATGGAGA 
     4021 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     4081 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     4141 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     4201 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     4261 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     4321 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     4381 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     4441 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     4501 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     4561 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     4621 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     4681 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4741 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4801 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4861 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4921 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4981 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5041 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5101 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5161 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5221 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5281 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5341 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     5401 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5461 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5521 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     5581 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     5641 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     5701 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     5761 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     5821 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     5881 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     5941 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     6001 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     6061 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     6121 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     6181 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     6241 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     6301 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     6361 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     6421 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     6481 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     6541 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     6601 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     6661 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     6721 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     6781 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     6841 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     6901 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     6961 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     7021 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     7081 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     7141 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     7201 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     7261 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     7321 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     7381 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     7441 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     7501 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     7561 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     7621 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     7681 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     7741 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     7801 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     7861 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     7921 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     7981 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     8041 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     8101 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     8161 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     8221 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     8281 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     8341 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     8401 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     8461 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     8521 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     8581 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     8641 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     8701 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     8761 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     8821 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     8881 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     8941 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     9001 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     9061 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     9121 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     9181 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     9241 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     9301 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     9361 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     9421 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     9481 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//