LOCUS       MG216058                9035 bp    DNA     circular  SYN 
DEFINITION  Trcg1 (tGFP-tagged) - Mouse taste receptor protein 1 (Trcg1), (10ug)
ACCESSION   NM_001014398
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3516..4220 
                     /label="GFP"  
     misc_feature    4267..4852 
                     /label="PolyA signal"  
     misc_feature    6944..7589 
                     /label="ColE1"  
     misc_feature    5306..6100 
                     /label="Neo"  
     misc_feature    4937..5239 
                     /label="SV40 ori"  
     misc_feature    7604..8464 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3495..3501 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3495 
                     /label="Trcg1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACAAGCAG TGGTTTCCTG CAGCTGGAAT TCTCTTGGCT GCCCTCCTAG 
     1081 TAGTCTCTGC TTCTACCCTG ACCCTTCTCT CTACTAATGA AGACCCTGAG CAGTTTCCCT 
     1141 CAGCCCCTGG CACATCAGCT CAGCAAAGTA GCCGCATTCT ACTGGGCATC CTGACAGACG 
     1201 TCACTGGTGG TATCAACTCA GTTGAGAGGG AACCGGAGGC CCTGGGGAGG AGGGCAGGAG 
     1261 GCCTCTCTAC AGAAGGAGCT GGGGGTCAGG AGTCTCCCTC AATGCCTGGC CCCTCAGGCA 
     1321 GGGTCATACC TGAACCAATT CCCTCAGCCC TGACCACATC TGCATCCGAC ATGGCCTCTC 
     1381 AGCCAGTGTC CTCTGGGGCT GACCCTATAG AGGAAATCAT GGCTCTTGGA ACTCTAGAGA 
     1441 CAATTACGAT GTCATCACCA CAGCCTTCTC CCAGACATGA ATCTGAGCAG AAGTTCGACA 
     1501 AGGTCTTCAG GTTGTGCGGG ACAGTTCCTG CCTCTGAAAC ACTGGAGAAA AATGTTGCAT 
     1561 CTAAACCAGC TTGGCATTTA ATTAAAGCTC CCCCTCCCCC CATATTCTCT AGATCTCCAC 
     1621 ACCTGTTATG GTGTACCCCC AATTCCACTG TCTACATACC AGTGCCTGCA TGGAGGGATG 
     1681 GCCACTCCAG GCCAGAGGCA TCCTCATCTG TGCCACTAGC TCCAAGTACC TCCTTAGGAC 
     1741 TGCCTATCTT TCCATGGATG CCTAACATAC TGAAAGCTAC AGAGTCCCTG TTGCCTGCAT 
     1801 CTCCTGGAAG ATCAGGGCTG GACCTCACCT CCCAAGTGGG CTCCAGAGCA TCTGAAAACA 
     1861 CCGTGGCTTT GGACACAGGC CCAGTATCCC GTGGTACTTC TGTGCTGCTG TTGCCCTCAG 
     1921 CAACATCCCC TTCACAGGCA TCCTCCCTCC ACTCCCCTCG CCCCTCATCA GCATCACCCC 
     1981 TGTCTGCATC CCCATCACCT GCAGCTTTGT CCTTCTTCCC ATCACCAGCC TCAATCCTGG 
     2041 TCCCGGTTTC CTCTGTCACC ACAGGAGCCA GTGACTCTCC TAAAGCTCCT GTGTCTGTCA 
     2101 TTGCACCCTC AACTACAGAC TCTTTCATTA AAACCTCAAA CCTCGGACCC CAGATAGCTC 
     2161 TACAACCTAG TCACCCTGGG CTATGGCTTC CCACCAGCCC AATCCACATG CCCACGCTCT 
     2221 CCCTCCAACA TTTCTCTAGC CCTCCCTCTA CCGCACATAG CTCTGGCTTC ACAGAGTCAT 
     2281 CTGTACATGC TGATCCTACC CTGGCCTCTA CCCTCCCTCA CCCTGGCCAG GATATGTCTT 
     2341 TGCAGGACTT GAGTTTCTCC ACTGGAGGAC GTAGTCATAC GACCCACTCT GTGACCTTTA 
     2401 GGATCAACAG CAATCGCTTC ACAAAAGCTG TCTGGAACCT GGTACCCTTG GAGCGCTGGC 
     2461 TGCTGAACAG GCTTATCTGC TACCAGCTCC GGTTCATCTA CCAGGAGGCC TTCCCCAACT 
     2521 TCAGGAATGT CAGCGCCCTG CTGTTTCGGC CTGGCTGTCC AGAGGTGAAA GCCTCCCTCA 
     2581 TTTTTGGTCC TCCGGATCCC TCGTCCATAG AAATCCTCTG GACTTTGTAC CGCAAAGTGA 
     2641 AGTCCTCCAG ATGGTCGCTT GGGTACCTGT CCCTGGCCGA CCATGGCCTT TCCTCTGATG 
     2701 GGTACAGCAT GACTGACCTT ACCCAGGAAA TCATCAACAT TAGCTTCACA CTCATGAGGC 
     2761 CCTTCCTGCC CCAGCTGCTT CTGCCTAGTT CTCAGTCTTG TATCCTGCTG GAAAAGCAGA 
     2821 CCATCCAGCT GGTCACCCAC GAAGTGTCAA GATTCTACAA GGCTGAGCTC CAGAGCCAGC 
     2881 CCCTGCTCCT ATTCAGCAAC GTGAAGGAGT GGGTGAGCGT TTACATGGAA TACAAATTCA 
     2941 AGAGCCCCAT CCCCATCCGT CTCCAAGGCC TGGCCAGTCA CCTGGCCCAT CATATAACAG 
     3001 ATCCCACCCT TCAGAAATCC AGCATAATGG CCAATGGGGA GAAAGCAGAT CTGGTGTTTT 
     3061 ATGAGATGTG GCTCTTGATC CTGGGTCACC CCTTCACCAA GACCTTGGAG AACAAGACTA 
     3121 GTTCTGAGTG CCAGGAGCTT CGTGGACTGC TGACGAGACA GCTAACCTCA GTCCTCCAGC 
     3181 CTTTGAAGAA CTTTGGTCAA GTGGTGGTGG AGGAATTCCA CCAGGAACCA CTGACTGCCA 
     3241 GGGTACAAAC GGCCTTCTTT GGGGCTGTGC CAGCCCAGGC CATCATTCAA GACACCGTGC 
     3301 TCCAAGCCCT GGGCTCCCTG CAGGAAACTG AGGGTCTGCA GTTAGAGATG CTCCTCCCAG 
     3361 TCCTTGGCAC CCCCAGCTCC AGAGCCTCGA GAGGCCCCAG GGGCGGGGCC ATGCTGAACC 
     3421 TCCAGCGCTT CACTTCTCTC TTTGTCCTGG TGGCTCTTTG TACGGCTCCT CCCTTCATCA 
     3481 ATAAGCAAGC CCTATACCTC TCCACGCGTA CGCGGCCGCT CGAGATGGAG AGCGACGAGA 
     3541 GCGGCCTGCC CGCCATGGAG ATCGAGTGCC GCATCACCGG CACCCTGAAC GGCGTGGAGT 
     3601 TCGAGCTGGT GGGCGGCGGA GAGGGCACCC CCGAGCAGGG CCGCATGACC AACAAGATGA 
     3661 AGAGCACCAA AGGCGCCCTG ACCTTCAGCC CCTACCTGCT GAGCCACGTG ATGGGCTACG 
     3721 GCTTCTACCA CTTCGGCACC TACCCCAGCG GCTACGAGAA CCCCTTCCTG CACGCCATCA 
     3781 ACAACGGCGG CTACACCAAC ACCCGCATCG AGAAGTACGA GGACGGCGGC GTGCTGCACG 
     3841 TGAGCTTCAG CTACCGCTAC GAGGCCGGCC GCGTGATCGG CGACTTCAAG GTGATGGGCA 
     3901 CCGGCTTCCC CGAGGACAGC GTGATCTTCA CCGACAAGAT CATCCGCAGC AACGCCACCG 
     3961 TGGAGCACCT GCACCCCATG GGCGATAACG ATCTGGATGG CAGCTTCACC CGCACCTTCA 
     4021 GCCTGCGCGA CGGCGGCTAC TACAGCTCCG TGGTGGACAG CCACATGCAC TTCAAGAGCG 
     4081 CCATCCACCC CAGCATCCTG CAGAACGGGG GCCCCATGTT CGCCTTCCGC CGCGTGGAGG 
     4141 AGGATCACAG CAACACCGAG CTGGGCATCG TGGAGTACCA GCACGCCTTC AAGACCCCGG 
     4201 ATGCAGATGC CGGTGAAGAA AGAGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4261 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4321 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4381 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4441 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4501 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4561 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4621 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4681 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4741 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4801 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4861 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCTTG GCTGTGGAAT 
     4921 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     4981 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     5041 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC 
     5101 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 
     5161 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA 
     5221 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC 
     5281 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC 
     5341 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA 
     5401 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT 
     5461 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG 
     5521 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA 
     5581 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 
     5641 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG 
     5701 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG 
     5761 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC 
     5821 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT 
     5881 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 
     5941 TGTGGCCGGC TGGGTGTGGC CGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT 
     6001 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT 
     6061 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC 
     6121 TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA 
     6181 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA 
     6241 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG 
     6301 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT 
     6361 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC 
     6421 CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT 
     6481 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG 
     6541 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT 
     6601 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT 
     6661 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 
     6721 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 
     6781 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 
     6841 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 
     6901 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 
     6961 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 
     7021 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG 
     7081 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 
     7141 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 
     7201 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 
     7261 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC 
     7321 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 
     7381 CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 
     7441 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 
     7501 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 
     7561 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 
     7621 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 
     7681 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 
     7741 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 
     7801 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 
     7861 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 
     7921 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 
     7981 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 
     8041 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 
     8101 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 
     8161 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 
     8221 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 
     8281 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 
     8341 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 
     8401 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 
     8461 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 
     8521 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 
     8581 CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 
     8641 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 
     8701 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 
     8761 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 
     8821 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 
     8881 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 
     8941 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 
     9001 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//