LOCUS       MG215880                9188 bp    DNA     circular  SYN 
DEFINITION  Dcaf6 (tGFP-tagged) - Mouse DDB1 and CUL4 associated factor 6 (Dcaf6), (10ug)
ACCESSION   NM_028759
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3669..4373 
                     /label="GFP"  
     misc_feature    4420..5005 
                     /label="PolyA signal"  
     misc_feature    7097..7742 
                     /label="ColE1"  
     misc_feature    5459..6253 
                     /label="Neo"  
     misc_feature    5090..5392 
                     /label="SV40 ori"  
     misc_feature    7757..8617 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3648..3654 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3648 
                     /label="Dcaf6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCGGAGT GGCTCCTGCC CGCACCTGTT GTGGGACGTG AGGAAAAGGT 
     1081 CCCTTGGGCT GGAGGACCCG TCCCGGCTGA GGAGCCGCTA CCTGGGAAGA AGAGAATTTA 
     1141 TCCAAAGATT AAAACTTGAA GCAACTTTAA ATGTGCATGA TGGCTGTGTT AATACAATCT 
     1201 GTTGGAATGA CACTGGAGAA TATATTTTAT CTGGCTCTGA TGACACTAAA CTTGTAATTA 
     1261 GTAATCCATA CAGCAGAAAG GTTTTGACAA CCATCCGTTC AGGGCATCGA GCAAATATAT 
     1321 TTAGTGCAAA GTTTTTGCCG TGCACAGATG ATAAGCAGAT TGTGTCTTGC TCTGGAGATG 
     1381 GAGTCATATT TTATACTAAC ATTGAGCAAG ATGCAGAAAC TAACAGACAG TGCCAATTTA 
     1441 CGTGCCATTA TGGAACTACT TATGAGATTA TGACTGTACC AAACGACCCT TACACCTTTC 
     1501 TGTCCTGTGG TGAAGATGGA ACTGTTAGGT GGTTTGACAC ACGCATCAAA ACCAGTTGCA 
     1561 CAAAAGAAGA CTGTAAAGAT GATATTTTAA TCAACTGTAG GCGTGCTGCC ACATCTGTGG 
     1621 CTATTTGTCC CCCAGTACCA TATTACCTTG CTGTGGGTTG TTCTGACAGC TCAGTACGGA 
     1681 TTTATGATCG GCGAATGCTG GGCACAAGAG CTACAGGGAA TTATGCAGGC CGAGGAACTA 
     1741 CTGGAATGGT TGCTCGATTT ATACCTTCTC ATCTTAGTAA CAAATCATGC AGAGTGACAT 
     1801 CACTGTGTTA CAGTGAAGAT GGTCAAGAGA TTCTTGTCAG TTATTCTTCA GACTACATCT 
     1861 ATCTTTTTGA CCCCAAAGAT GATACTGCAC GAGAACTTAA AACTCCTTCT GCAGAGGAGA 
     1921 GGCGAGAAGA GTTACGACAG CCTCCAGTTA AGCGCTTGAG ACTTCGTGGT GATTGGTCAG 
     1981 ATACTGGTCC CAGAGCACGG CCAGAAAGTG AACGAGAACG AGATGGAGAG CAAAGTCCCA 
     2041 ATGTGTCACT GATGCAGAGA ATGTCTGATA TGTTATCAAG GTGGTTTGAA GAAGCAAGTG 
     2101 AAGTTGCACA AAGCAACAGA GGAAGAGGAA GACCTCGGCC CAGAGGTGGA ACAAATCAGC 
     2161 CAGATGTTTC AACTCTTCCT ACGGTTCCAT CAAGTCCTAA TTTGGAAGTG TGTGAAACTG 
     2221 CAATGGATGT AGACATGCCA GCTGCACTTC TTCAGCCTTC TACATCCTCT ACAGATCCAG 
     2281 TTCAGGCTCA GGCAGCCACA GCCGCCATAG AAAGCCCTCG TTCCAGCTCG TTGCTGTCTT 
     2341 GCCCAGACAG TGAACCGAGG CAGTCTGTTG AGGCGTCTGG ACACCATGCA CATCATCAGT 
     2401 CAGATAACAG TAATGAGAGG CTGAGCCCCA AACCAGGGAC AGGTGAACCC GTTTTAAGTT 
     2461 TGCACTACAG CACAGAAGGA ACAACTACAA GCACAATAAA ACTGAACTTT ACAGATGAAT 
     2521 GGAGCAGTAC AGCCTCAAGT TCCAGAGGAA ATGGGAGCCA TTGCAAATCT GAGGGTCAGG 
     2581 AAGAATGCTT GGTCCCTCCG AGCTCTGTGC AGCCACCGGA AGGAGACAGT GAAACAAGAG 
     2641 CTCCTGAAGA ACTATCAGAG AAAGGAACAC TTCCAGAAAA CCTCACTCAA AACCAGATAG 
     2701 ATACAGCACA ACTTGATAAC TTCCCAGCTG AGCCATTGGA TTCTAACTCA GGAGAGAAGA 
     2761 ATAACCCAAG TCAGGACAGC CCTTGTGGGC TTCCAGAAGA AGGCACTTTG TCTGAAACAG 
     2821 ACAGGGAGAC TTGTGAGCAG GCCAGCACTG AGAGTGCTAC CAGGCATGCT AGCACCAAGC 
     2881 CTGAACTCCC ATCCCAGACA GAAGCCATTG AGCAGGCCAG CACTGAGAGT GCTACCAGGC 
     2941 ATACCAGTGC CAATCCTGAA CTCCCATCCC AGACAGAAGC CATAGCACCT TTAGCTCATG 
     3001 AAGACCCATC TGCCAGGGAC TCTGCTCTCC AGGACACAGA TGACAGCGAT GATGATCCGG 
     3061 TCTTGATCCC TGGTGCAAGA TACCGAACAG GACCTGGTGA TAGACGCTCC GCTGTTGCCC 
     3121 GCATTCAGGA GTTCTTCAGG AGGAGAAAAG AAAGGAAAGA AATGGAAGAG CTGGATACTT 
     3181 TGAACATTAG GAGGCCACTA GTAAAGATGG TTTATAAGGG CCACCGCAAC TCCCGGACAA 
     3241 TGATAAAAGA AGCCAATTTC TGGGGTGCTA ACTTTGTAAT GAGCGGTTCC GATTGTGGCC 
     3301 ATATCTTCAT CTGGGACCGG CACACTGCTG AGCATTTGAT GCTTCTGGAA GCTGATAATC 
     3361 ATGTGGTCAA CTGCCTGCAG CCCCATCCGT TTGACCCAAT TCTAGCCTCA TCTGGCATAG 
     3421 ATTATGACAT AAAGATCTGG TCGCCACTAG AAGAGTCAAG AATTTTTAAT CGAAAACTTG 
     3481 CTGATGAAGT TATAACTCGG AATGAACTCA TGCTGGAAGA GACTCGGAAC ACCATCACCG 
     3541 TCCCAGCCTC TTTCATGTTG AGGATGTTGG CGTCACTGAA TCATATCCGA GCTGACCGTC 
     3601 TGGAGGGTGA CAGATCAGAA GGTTCAGGTC AGGAGAATGA AAATGAGGAT GAAGAAACGC 
     3661 GTACGCGGCC GCTCGAGATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT 
     3721 GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA 
     3781 CCCCCGAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA 
     3841 GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA 
     3901 GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA 
     3961 TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG 
     4021 GCCGCGTGAT CGGCGACTTC AAGGTGATGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT 
     4081 TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA 
     4141 ACGATCTGGA TGGCAGCTTC ACCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT 
     4201 CCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG 
     4261 GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGGATCA CAGCAACACC GAGCTGGGCA 
     4321 TCGTGGAGTA CCAGCACGCC TTCAAGACCC CGGATGCAGA TGCCGGTGAA GAAAGAGTTT 
     4381 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4441 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4501 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4561 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4621 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4681 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4741 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4801 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4861 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4921 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4981 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5041 GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 
     5101 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 
     5161 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5221 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 
     5281 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 
     5341 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 
     5401 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 
     5461 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 
     5521 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 
     5581 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 
     5641 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 
     5701 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 
     5761 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 
     5821 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 
     5881 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 
     5941 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 
     6001 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 
     6061 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCCGACCGC 
     6121 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 
     6181 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 
     6241 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 
     6301 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 
     6361 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 
     6421 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 
     6481 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 
     6541 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 
     6601 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 
     6661 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 
     6721 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 
     6781 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT 
     6841 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 
     6901 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 
     6961 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 
     7021 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 
     7081 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 
     7141 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 
     7201 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 
     7261 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 
     7321 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 
     7381 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 
     7441 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 
     7501 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA 
     7561 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 
     7621 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 
     7681 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
     7741 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 
     7801 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 
     7861 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 
     7921 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 
     7981 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
     8041 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 
     8101 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 
     8161 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 
     8221 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 
     8281 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
     8341 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 
     8401 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 
     8461 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 
     8521 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 
     8581 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
     8641 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     8701 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     8761 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     8821 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     8881 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     8941 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     9001 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     9061 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     9121 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     9181 CGAATTTT
//