LOCUS       MG215858                9473 bp    DNA     circular  SYN 
DEFINITION  Cse1l (tGFP-tagged) - Mouse chromosome segregation 1-like (S. cerevisiae) (Cse1l), (10ug)
ACCESSION   NM_023565
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3954..4658 
                     /label="GFP"  
     misc_feature    4705..5290 
                     /label="PolyA signal"  
     misc_feature    7382..8027 
                     /label="ColE1"  
     misc_feature    5744..6538 
                     /label="Neo"  
     misc_feature    5375..5677 
                     /label="SV40 ori"  
     misc_feature    8042..8902 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3933..3939 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3933 
                     /label="Cse1l"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTCAGC GATGCGAATT TACAGACACT AACAGAGTAT TTAAAGAAAA 
     1081 CCCTGGATCC TGATCCTGCC ATTAGACGGC CAGCTGAGAA GTTTCTCGAA TCCGTAGAAG 
     1141 GAAATCAGAA TTACCCACTG TTGCTTTTAA CATTATTGGA AAAGTCACAG GATAATGTCA 
     1201 TTAAAGTATG TGCTTCAGTA ACCTTCAAGA ACTATATTAA AAGGAATTGG AGAATTGTTG 
     1261 AAGATGAACC AAACAAAATT TGCGAAGCTG ATCGAGTAGC TATTAAAGCC AACATAGTGC 
     1321 ATCTGATGCT GAGCAGTCCA GAGCAGATTC AGAAGCAGCT GAGTGATGCT ATTAGCATCA 
     1381 TTGGCAGAGA AGATTTCCCT CAGAAATGGC CTGACTTGTT GACAGAAATG GTGAATCGCT 
     1441 TTCAGAGTGG AGATTTTCAC GTCATTAATG GAGTCCTTCG TACAGCGCAC TCACTATTTA 
     1501 AGAGATACCG GCATGAGTTC AAGTCAAATG AGCTGTGGAC TGAAATTAAA CTTGTCTTGG 
     1561 ATGCCTTTGC TTTGCCTTTG ACTAATCTAT TCAAGGCCAC CATTGAACTG TGCAGTACCC 
     1621 ATGCAAATGA CGCCTCTGCC CTGAGGATTC TTTTTTCTTC CTTGATTTTG ATCTCAAAAT 
     1681 TGTTCTATAG TTTGAACTTT CAGGATCTCC CTGAATTCTT TGAAGATAAT ATGGAAACTT 
     1741 GGATGAACAA TTTTCATACC CTCCTGACAC TGGATAATAA GCTCTTGCAG ACTGATGATG 
     1801 AAGAGGAAGC AGGCTTACTG GAGCTTTTAA AGTCTCAGAT CTGTGATAAT GCCGCTCTCT 
     1861 ATGCACAGAA ATATGACGAA GAGTTTCAGC GGTACCTCCC TCGCTTTGTC ACAGCTATCT 
     1921 GGAACTTACT CGTTACAACA GGGCGAGAAG TTAAATATGA CTTGTTGGTT AGCAATGCAA 
     1981 TTCAGTTTCT GGCTTCAGTT TGTGAAAGGC CACATTATAA GAATCTGTTT GAGGACCAGA 
     2041 ACACGCTGAC AAGTATCTGT GAAAAAGTTA TTGTGCCTAA CATGGAATTT AGAGCTGCTG 
     2101 ATGAAGAAGC TTTTGAAGAT AATTCTGAGG AGTACATACG GAGGGATTTG GAAGGCTCTG 
     2161 ATATTGATAC TAGACGCAGG GCTGCTTGTG ATCTTGTACG AGGATTATGC AAATTTTTTG 
     2221 AAGGTCCTGT GACAGGAATC TTCTCTGGTT ATGTTAATTC CATGCTCCAG GAGTATGCAA 
     2281 AAAACCCGTC TGTCAACTGG AAACACAAAG ATGCAGCTAT TTACCTTGTC ACATCTTTGG 
     2341 CATCAAAAGC CCAGACACAG AAGCATGGAA TCACGCAAGC CAATGAACTT GTAAACCTAA 
     2401 CTGAATTTTT CGTGAATCAC ATACTTCCTG ATTTAAAATC AAATAATGTG AATGAGTTTC 
     2461 CTGTGCTTAA AGCTGATGGT ATCAAATATA TTATGATTTT TAGGAATCAA GTACCAAAAG 
     2521 AGCACCTCCT AGTCTCTATT CCCCTCTTGA TCAGTCACCT GGAGGCTGAG AGTATTGTCG 
     2581 TCCACACTTA TGCCGCACAT GCTCTCGAAA GGCTGTTCAC TATGAGAGGG TCAAACAATA 
     2641 CCACTCTTTT TACAGCTGCA GAAATCGCAC CGTTTGTTGA GATTCTGCTA ACAAACCTTT 
     2701 TCAAAGCTCT CACACTTCCT GGCTCTTCAG AAAATGAATA TATTATGAAA GCTATCATGA 
     2761 GAAGTTTCTC CCTCCTGCAA GAGGCCATCA TCCCCTATAT CCCCACTCTC ATCACGCAGC 
     2821 TCACACAGAA GCTGCTGGCT GTTAGTAAGA ATCCCAGTAA ACCTCATTTC AATCACTACA 
     2881 TGTTTGAAGC AATATGCTTG TCTATAAGAA TAACCTGCAA AGCTAACCCT GCTGCTGTTG 
     2941 TGAATTTTGA GGAGGCTCTG TTTCTAGTGT TCACTGAGAT CTTACAAAAT GATGTACAAG 
     3001 AGTTTATCCC ATATGTCTTT CAAGTGATGT CTTTACTTCT GGAAACACAT AAAAATGATA 
     3061 TCCCGTCTTC CTATATGGCC TTGTTTCCCC ACCTCCTTCA GCCAGTACTG TGGGAAAGGA 
     3121 CAGGAAACAT CCCTGCTCTA GTGAGGCTGC TCCAGGCATT CTTAGAACGA GGCTCAAGTA 
     3181 CAATAGCAAC TGCTGCAGCT GACAAAATTC CTGGATTACT GGGTGTCTTC CAGAAGCTGA 
     3241 TTGCATCCAA GGCAAACGAT CACCAAGGTT TCTATCTTCT CAACAGTATA ATCGAACACA 
     3301 TGCCTCCTGA ATCAGTTGAC CAGTATAGGA AGCAGATCTT CATTCTACTA TTCCAGAGAC 
     3361 TGCAAAACTC CAAGACCACC AAGTTTATCA AGAGTTTCTT AGTGTTTATT AACTTATATT 
     3421 GCATTAAGTA TGGGGCACTA GCATTACAAG AAATATTTGA TGGCATACAA CCAAAAATGT 
     3481 TTGGAATGGT TTTGGAAAAA ATCATTATTC CAGAGATTCA AAAGGTATCT GGGAACGTGG 
     3541 AGAAAAAAAT CTGTGCAGTT GGCATAACCA AATTACTCAC AGAGTGTCCA CCAATGATGG 
     3601 ACACGGAGTA CACTAAGCTG TGGACTCCTT TGTTACAATC TCTCATTGGC CTGTTTGAAT 
     3661 TACCTGAAGA TGACAGCATT CCTGATGAAG AGCATTTCAT TGACATAGAA GATACACCAG 
     3721 GATACCAGAC TGCCTTCTCA CAGCTGGCAT TTGCTGGTAA AAAGGAACAT GATCCTGTAG 
     3781 GTCAGATGGT AAACAACCCC AAAATCCATC TGGCACAGTC ACTTCACAAA CTGTCAACTG 
     3841 CTTGTCCTGG AAGGGTTCCC TCGATGGTGA GCACCAGCCT CAATGCAGAA GCCTTGCAGT 
     3901 ATCTCCAAGG TTACCTGCAG GCAGCCAGTG TGACCCTGTT GACGCGTACG CGGCCGCTCG 
     3961 AGATGGAGAG CGACGAGAGC GGCCTGCCCG CCATGGAGAT CGAGTGCCGC ATCACCGGCA 
     4021 CCCTGAACGG CGTGGAGTTC GAGCTGGTGG GCGGCGGAGA GGGCACCCCC GAGCAGGGCC 
     4081 GCATGACCAA CAAGATGAAG AGCACCAAAG GCGCCCTGAC CTTCAGCCCC TACCTGCTGA 
     4141 GCCACGTGAT GGGCTACGGC TTCTACCACT TCGGCACCTA CCCCAGCGGC TACGAGAACC 
     4201 CCTTCCTGCA CGCCATCAAC AACGGCGGCT ACACCAACAC CCGCATCGAG AAGTACGAGG 
     4261 ACGGCGGCGT GCTGCACGTG AGCTTCAGCT ACCGCTACGA GGCCGGCCGC GTGATCGGCG 
     4321 ACTTCAAGGT GATGGGCACC GGCTTCCCCG AGGACAGCGT GATCTTCACC GACAAGATCA 
     4381 TCCGCAGCAA CGCCACCGTG GAGCACCTGC ACCCCATGGG CGATAACGAT CTGGATGGCA 
     4441 GCTTCACCCG CACCTTCAGC CTGCGCGACG GCGGCTACTA CAGCTCCGTG GTGGACAGCC 
     4501 ACATGCACTT CAAGAGCGCC ATCCACCCCA GCATCCTGCA GAACGGGGGC CCCATGTTCG 
     4561 CCTTCCGCCG CGTGGAGGAG GATCACAGCA ACACCGAGCT GGGCATCGTG GAGTACCAGC 
     4621 ACGCCTTCAA GACCCCGGAT GCAGATGCCG GTGAAGAAAG AGTTTAAACG GCCGGCCGCG 
     4681 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4741 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4801 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4861 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4921 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4981 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5041 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5101 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5161 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5221 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5281 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5341 CCAGCTTGGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     5401 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA 
     5461 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC 
     5521 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC 
     5581 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA 
     5641 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA 
     5701 GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT 
     5761 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT 
     5821 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG 
     5881 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC 
     5941 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC 
     6001 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC 
     6061 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG 
     6121 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG 
     6181 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT 
     6241 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG 
     6301 GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC 
     6361 TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCCG ACCGCTATCA GGACATAGCG 
     6421 TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG 
     6481 CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG 
     6541 TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT 
     6601 CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC 
     6661 GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC 
     6721 CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA 
     6781 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT 
     6841 CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC 
     6901 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA 
     6961 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 
     7021 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 
     7081 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 
     7141 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 
     7201 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 
     7261 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 
     7321 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 
     7381 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 
     7441 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 
     7501 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 
     7561 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 
     7621 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 
     7681 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG 
     7741 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 
     7801 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 
     7861 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 
     7921 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 
     7981 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 
     8041 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC 
     8101 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC 
     8161 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT 
     8221 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG 
     8281 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA 
     8341 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA 
     8401 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT 
     8461 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG 
     8521 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA 
     8581 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC 
     8641 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT 
     8701 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC 
     8761 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA 
     8821 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA 
     8881 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 
     8941 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 
     9001 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG 
     9061 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC 
     9121 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC 
     9181 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG 
     9241 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG 
     9301 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG 
     9361 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT 
     9421 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//