LOCUS       MG215184                9020 bp    DNA     circular  SYN 
DEFINITION  Svs1 (tGFP-tagged) - Mouse seminal vesicle secretory protein 1 (Svs1), (10ug). Note: ORF is codon optimized
ACCESSION   NM_172888
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3501..4205 
                     /label="GFP"  
     misc_feature    4252..4837 
                     /label="PolyA signal"  
     misc_feature    6929..7574 
                     /label="ColE1"  
     misc_feature    5291..6085 
                     /label="Neo"  
     misc_feature    4922..5224 
                     /label="SV40 ori"  
     misc_feature    7589..8449 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3480..3486 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3480 
                     /label="Svs1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCTTGGTC GGGCGAACCC TTGTGCTGGG GTGGATGACA ACGATTCTCG 
     1081 TTGTGCACAT GAGCGTGGCT AAACACTCCA GGTGGGCCCT GAGAACAAAG CCCCAGATGT 
     1141 TGTGGGACCT CTCTGGAGAG GAACTGGAGG CCGTGCACAA CTTTGTTATG TCCTCAAAGG 
     1201 AGCTCGAGCT GCAGCCTTCA ACTCTGCTTA CTCTCGCAAA GAATTCCGTC TTCCTGATAG 
     1261 AAATGCTGAT GCCCAAGAAA TACGAGGTTC TTGCGTTCCT GGATAAAGGC GCAATGCTTC 
     1321 CCCTGAGAGA AGCTAGAGTG CTGATCTACT TCGGAGCCCA GGAGTATCCA AACGTGACCG 
     1381 AATACGCCGT GGGCCCAGTC GAACAGCCCA TGTATATGAG GAAACTCAAC CGAAAAGGCG 
     1441 GTCAGCAGCT CTCCTGGGCA TCACGACCAA TGTCTAAGGT AGAGAGTGCA TTGCTTCTGC 
     1501 ACACTTTGAA GACAGCCACT AAGCCGCTCC AGGAATTTTT TTTCGACACA ACCGGATTCA 
     1561 CCCTGCAGGA TTGCAATGGT GGATGCTTGA CTTTTACGAA TGTCGGCCCT AGGGCCATGA 
     1621 CCTTTGGAAA GAGGCATTCC TGGTTCCTGC TTCAGCGGAT AATGAATGAC TATTTCCTGC 
     1681 AGCCCACCGG ACTTGAAATC CTGTTGGACC ATGGGAGCAC GGATGTCCAG GATTGGATCG 
     1741 TGGAACAGGT TTGGTATAAC GGTAAGTTCT ATAACTCCCC AGAGGAACTC GCTCAGAAGT 
     1801 ACGCCGATGG GGAAGTGGAC ATCGTAGTGC TCGAAGACCC ACTCCCCAAA ACCACAGAAC 
     1861 AGTTCCCACA GTTTTCCACC TATAAGCCAT ACGCCGAGTT TTTGATGCCA ATCAGCAAAG 
     1921 GGGGACCACG GGTCATCCAG CTGTACCCAG AATTCCCCAT GCCTATCTCT AAAGGTGGGC 
     1981 CCCAGAAGGC TCAACCCGAT CCTAAATTTC CCATTCCCAT TAGTAAAGGA GGCCCCCAGG 
     2041 TGGCACAGCC CTATAGAGAA TTTCCCATGC CTATTTCTAA GGGGGGGCCA CAGGTCGTCC 
     2101 AGCCCGATCC CAAATTCCCA ATTCCAATTT CTAAGGGAGG CCCTAGGGTA CCACAGCCCC 
     2161 ACGCCAAGTT CCCAATGCCA ATCTCAAAAG GGGGCCCACG GGTCGTGCAG CCCAAGGCGA 
     2221 AGTTTCCAAT GCCCATATCC AAGGGCGGCC CGCAAATAGC TCAGCCCCAT GTGCCGCACT 
     2281 ACAGGCTGAA GCACAATACA GTTTTGTACG GAGACTGGAA CTTCTTTTTC AAGCTGCACT 
     2341 CAAGTTACGG CTTGCAGCTC TTCAATGTCC ATTTCCGCGG AGAACGCATA GCCTATGAAG 
     2401 TCGGGGTACA GGAGGTGATG GCCTTGTACA GGGGCCATAC AGCCGCCGGT AGGAAGACCA 
     2461 AGTACATGGA TGTAGGGTGG GGTCTTGGAG GTATTACCCA CCAGCTGACC CCGGGGATTG 
     2521 ACTGTCCTCA TGAGGCTACC TTCCTGGACG CTATACACTA CTATGATTCT GACGGTCCAG 
     2581 TGCTTAGCAG GCGCGCCCTG TGCATATTCG AAATGCCGTT GCGCCAATAT TTCAATAGCA 
     2641 ACTTTCGATC CTCCTTCTCA TCATACGCAA AGCTGTCAGG ACCAATGCTG GTACTTCGGA 
     2701 CCGCATCAAC CATCCACAAT CACGACTATA TTTGGGATTT CATCTTCCAC TCAAACGGAA 
     2761 TGATGGAAGG AAAGATGTAT GCGACAGGCT ATGTGCACGC AACTTTCTAT TCTTCAGAGG 
     2821 GCCTTCTGTA TCACTCCAGG TTGCACACCC ACCTGCTCGG AAATGTGCAT AGCCACCTGG 
     2881 CCCATTATAG GATCGATCTG GATGTGGCTG GTACCAAGAA CAGGTTCCAG ACTCTGAAGA 
     2941 TGCGCTTGGA AAATATCATG GACCCCTGGT CCCAGCAGGT GAAGCCTATT CTCGATAAAA 
     3001 CCCAGTATAG TTGGGAGCGC CAGGCCGCAT TCCACTTCAG ACAGACCCTT CCCAAATACC 
     3061 TCCTGTTTTC TAATACTGGG AAGTCTGTGA GCGGACTGTC ACATTCTTAC CGCCTTCACG 
     3121 TGCCCTCTAT GGCTGAACAA GTGCTGCCAC CAGGCTGGCA GACCAGTCCT GCATTCACAT 
     3181 GGCCACGATA TCAGTTGGCT GTCACCAAGT ATCAGGAGAG TGAGAGGTTT CACGGCTCCC 
     3241 TCTATAACCA GAACCATCAC TGGGCCTACC CCATGGTGTT CGAAAACTTC ATCCACAACA 
     3301 ACGAGAATAT TGAGGACGAG GATCTTGTAG CTTGGGTAAC CGTTGGCCTC TCCCACAACC 
     3361 ACCACTCAGA AATCGTGCCT AGCGTCGCTA CCCCTGGCAA CTCCGCAGGC TTCCTGCTGC 
     3421 AGCCGTTTGA TTTCTATAAT TCTTTCAGGC GCTATACTGC TTCTCCAACA CACGCTCAGT 
     3481 GTGTGTGTAC GCGTACGCGG CCGCTCGAGA TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA 
     3541 TGGAGATCGA GTGCCGCATC ACCGGCACCC TGAACGGCGT GGAGTTCGAG CTGGTGGGCG 
     3601 GCGGAGAGGG CACCCCCGAG CAGGGCCGCA TGACCAACAA GATGAAGAGC ACCAAAGGCG 
     3661 CCCTGACCTT CAGCCCCTAC CTGCTGAGCC ACGTGATGGG CTACGGCTTC TACCACTTCG 
     3721 GCACCTACCC CAGCGGCTAC GAGAACCCCT TCCTGCACGC CATCAACAAC GGCGGCTACA 
     3781 CCAACACCCG CATCGAGAAG TACGAGGACG GCGGCGTGCT GCACGTGAGC TTCAGCTACC 
     3841 GCTACGAGGC CGGCCGCGTG ATCGGCGACT TCAAGGTGAT GGGCACCGGC TTCCCCGAGG 
     3901 ACAGCGTGAT CTTCACCGAC AAGATCATCC GCAGCAACGC CACCGTGGAG CACCTGCACC 
     3961 CCATGGGCGA TAACGATCTG GATGGCAGCT TCACCCGCAC CTTCAGCCTG CGCGACGGCG 
     4021 GCTACTACAG CTCCGTGGTG GACAGCCACA TGCACTTCAA GAGCGCCATC CACCCCAGCA 
     4081 TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT TCCGCCGCGT GGAGGAGGAT CACAGCAACA 
     4141 CCGAGCTGGG CATCGTGGAG TACCAGCACG CCTTCAAGAC CCCGGATGCA GATGCCGGTG 
     4201 AAGAAAGAGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4261 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4321 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4381 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4441 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4501 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4561 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4621 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4681 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4741 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4801 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4861 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCTTGGCTGT GGAATGTGTG TCAGTTAGGG 
     4921 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     4981 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5041 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 
     5101 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 
     5161 GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 
     5221 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 
     5281 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 
     5341 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 
     5401 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 
     5461 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 
     5521 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 
     5581 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 
     5641 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 
     5701 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 
     5761 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 
     5821 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 
     5881 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 
     5941 GTGGCCGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 
     6001 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 
     6061 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA 
     6121 CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG 
     6181 AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG 
     6241 ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 
     6301 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 
     6361 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT 
     6421 AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA 
     6481 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 
     6541 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 
     6601 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT 
     6661 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 
     6721 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 
     6781 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 
     6841 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 
     6901 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 
     6961 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 
     7021 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 
     7081 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 
     7141 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 
     7201 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 
     7261 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 
     7321 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT 
     7381 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 
     7441 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 
     7501 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 
     7561 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 
     7621 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 
     7681 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 
     7741 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 
     7801 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 
     7861 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 
     7921 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 
     7981 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 
     8041 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 
     8101 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 
     8161 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 
     8221 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 
     8281 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 
     8341 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 
     8401 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 
     8461 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 
     8521 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 
     8581 TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA 
     8641 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT 
     8701 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT 
     8761 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC 
     8821 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT 
     8881 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT 
     8941 TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC 
     9001 AAAAATTTAA CGCGAATTTT 
//