LOCUS       MG215046                9314 bp    DNA     circular  SYN 
DEFINITION  Nfxl1 (tGFP-tagged) - Mouse nuclear transcription factor X-box binding-like 1 (Nfxl1), (10ug)
ACCESSION   NM_133921
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3795..4499 
                     /label="GFP"  
     misc_feature    4546..5131 
                     /label="PolyA signal"  
     misc_feature    7223..7868 
                     /label="ColE1"  
     misc_feature    5585..6379 
                     /label="Neo"  
     misc_feature    5216..5518 
                     /label="SV40 ori"  
     misc_feature    7883..8743 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3774..3780 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3774 
                     /label="Nfxl1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACGCTCCC TGGCGCCAGG TGGCCGCTGG CCGAGGCCGA GCCCGAGGAC 
     1081 GGGCCACCGC AGTGCCCCTC TCAGGAAATG GAGTCCCTTT TGGTGGCGCT GGAGGAGGGC 
     1141 GAGCCAAAGG GTCTGGGGGC GCGGTGTCTT CGGGCCCCGG CCCCGGGGGA GCAGCAGCTC 
     1201 TGGCCCCTGC GGGTCGCGGG CAGCGCTCAG CTGGAGGCGA AGCCCCGCAG CCCCCGGGGG 
     1261 CCCGGGGTGA GGATGGCTCA GAACTAATGT CTCAGAAGAA ATTTGAGGAG ATCAAGAAGT 
     1321 CCAATCAAGC TGCAGCCAGA AAGCTGTTGG AGGAGCACTG CAGCTCTTCC TCTGAAGAAG 
     1381 AAGGGGATGA AGACTTGGAA GGAAAACAGG GGAAAATTGT TGCTAATACA TTCACAACAT 
     1441 ACACTACTCA CACAGATGGA GATATACATG AATTAGAGCG AACAAAACAG TATCTGAATG 
     1501 AGGCTTTCCA AGCAGGGGCT ATGACGTGCC TAATTTGTAT TGCTTCAGTG AAGAGAAACC 
     1561 AGGCAGTTTG GAGCTGTTCA GGATGTTTCT GTATATTCCA TATGCCCTGT ATTCAGAAGT 
     1621 GGGCTAAAGA CAGCCAGTTT CTTGTGTCTT CTGTGACAGA CGATGATTTT GGAAAGAAAG 
     1681 ATTATCCCTG GCCATGTCCA AAATGTAGAT TTGAATACAA AAGATCAGAA ACACCTAGTA 
     1741 GATATTATTG CTACTGTGGA AAAGTAGAAG ATCCACCTTT AGATCCGTGG CTTGTACCTC 
     1801 ATTCGTGTGG CCAAGTATGT GAGAGAGAAT TTAAACCTTC TTGTGGTCAT AAATGCTTGC 
     1861 TGCTCTGCCA TCCAGGTCCC TGCCCTCCTT GTCCAAAGAT GGTCACAACC ACTTGTTACT 
     1921 GTAAGAAAGC CAAGCCTATC CCTCGCAGGT GCAGTGCCAA GCAGTGGTCC TGTCAGCTGC 
     1981 CATGTGGGCG AAAGCTGCTT TGTGGACATC ATAAGTGTGA AAATCCTTGC CATGCAGGAA 
     2041 ACTGTCAGCC TTGTCCAAGA GTTAGTAGAC AAAAATGTGT ATGTGGCAAA AAAGTAGCAG 
     2101 AAAGAAGTTG TGCAAGCCCA GTGTGGCATT GTGATCAAGT ATGTGGAAAG ACACTGCCAT 
     2161 GTGGTAATCA TACATGTGAG CAAGTTTGTC ATGTTGGTGC TTGTGGAGAG TGTCCTCGAT 
     2221 CTGGGAAGAG GTCCTGTCCA TGTCAGAAAT CAAAGTTTTC TTTGCCTTGT ACAGAAGATG 
     2281 TACCAACTTG TGGAGACAGC TGTGACAAAA TACTTGAATG TGGAATCCAT AGGTGTTCAC 
     2341 AGCGTTGCCA CCGAGGTCCT TGTGAAACAT GCAGACAAGA AGTGGAAAAA CAGTGTCGTT 
     2401 GTGGAAAGCA CACAAAGCGA ATGCCTTGTC ACAAGCCTTA CCTTTGTGAA ACAAAGTGTG 
     2461 TTAAAATGCG GGACTGTCAA AAGCATCAGT GTAGGAGAAA GTGTTGCCCT GGAAACTGTC 
     2521 CACCTTGTGA TCAAAATTGT GGACGGACTT TAGGATGTAG AAACCATAAG TGTCCGTCTG 
     2581 TCTGCCATAG AGGCAGTTGC TATCCATGTC CAGAAACGGT AGATGTGAAG TGCAATTGTG 
     2641 GTAATGCAAA GGTGACAGTA CCCTGTGGCC GAGAGCGGAC CACAAGGCCA CCTAAGTGCA 
     2701 AAGAGCTGTG CAGTCGACCA CCAACATGTC ATCATGCAAG TCAAGAAAAA CACCGCTGTC 
     2761 ACTTTGGCCC ATGTCCACCG TGCCATCAGC CTTGCCAAAA AGTTCTGGAG AAGTGTGGGC 
     2821 ACTTGTGTCC TGAGTCATGC CATGATCAAG CATTAGTAAA GCAGACTGGC AGGCATCAGC 
     2881 CATCAGGCCC TTGGGAGCAG CCAGCTGAGC CTGCATTCAT CCAGACGGCC CTGCCTTGCC 
     2941 CTCCGTGTCA AGTTCCCATC CCTGTGGAAT GTCTGGGGAA GCATGAGGTG AGCCCACTAC 
     3001 CATGCCATGC TGTAGGACCC TATTCTTGCA AGAGACTTTG TGGAAGAATG TTGCGCTGTC 
     3061 AGAATCATAC ATGTATGAAA GAGTGCCACA AAGTTACTGA AGTTGATAGC TCTACTGGCA 
     3121 AAAATAAGGC TGGCCCAGAA TGCTTTCACT GTGAAGAAGG GTGCTCTAAG TCACGGCCTG 
     3181 CGGGTTGTCC TCACCCTTGT GTTTTGCCAT GTCACCCTGG AAAATGCCCT CCTTGTGTCC 
     3241 AGATGCTTAG AATAAAATGT CACTGCAAGA TCACAAGCCT GTATGTGGAA TGTAGAAAAA 
     3301 TAACCACAGC TGATGTAAGC GAAAAGAACC TCCTCAGTTG CTGCAAAAAC CAGTGCCCCA 
     3361 AAGAGCTTCC TTGTGGCCAC AGATGCAAAG AGATGTGCCA TCCTGGTGAA TGCCCCTTTA 
     3421 ATTGCAACCA GAAGGTAAAA CTTAGATGCC CTTGTAAAAG AATAAAACGG GAGTTGCCTT 
     3481 GCAATAAAGT ACGTGAAAAT CAGGTTTCAG TTGAATGTGA TGCAACGTGC AAGGAGATGA 
     3541 AGCGGAAAGC GTCTGAGGTT AAAGAAGCAG AAGCCAACGC TGCCCTGGAA GAAGAAAAGC 
     3601 GAAGGCAGCA GGCTGAACTG GAGGCTTTTG AAAACAGACT GAAGGGCCGT CGGAAGAAGA 
     3661 GCAGGAAACG CGATGAAGTG GCCGTGGAGC TCTCGCCTTG GCAGAAGTAT AAGTCCTACC 
     3721 TCCTTCCCGT GTGTGCAGTT GTGGTGGCAG TGCTTATGTG GTACATCTTC CATGGTGTGG 
     3781 ATACGCGTAC GCGGCCGCTC GAGATGGAGA GCGACGAGAG CGGCCTGCCC GCCATGGAGA 
     3841 TCGAGTGCCG CATCACCGGC ACCCTGAACG GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG 
     3901 AGGGCACCCC CGAGCAGGGC CGCATGACCA ACAAGATGAA GAGCACCAAA GGCGCCCTGA 
     3961 CCTTCAGCCC CTACCTGCTG AGCCACGTGA TGGGCTACGG CTTCTACCAC TTCGGCACCT 
     4021 ACCCCAGCGG CTACGAGAAC CCCTTCCTGC ACGCCATCAA CAACGGCGGC TACACCAACA 
     4081 CCCGCATCGA GAAGTACGAG GACGGCGGCG TGCTGCACGT GAGCTTCAGC TACCGCTACG 
     4141 AGGCCGGCCG CGTGATCGGC GACTTCAAGG TGATGGGCAC CGGCTTCCCC GAGGACAGCG 
     4201 TGATCTTCAC CGACAAGATC ATCCGCAGCA ACGCCACCGT GGAGCACCTG CACCCCATGG 
     4261 GCGATAACGA TCTGGATGGC AGCTTCACCC GCACCTTCAG CCTGCGCGAC GGCGGCTACT 
     4321 ACAGCTCCGT GGTGGACAGC CACATGCACT TCAAGAGCGC CATCCACCCC AGCATCCTGC 
     4381 AGAACGGGGG CCCCATGTTC GCCTTCCGCC GCGTGGAGGA GGATCACAGC AACACCGAGC 
     4441 TGGGCATCGT GGAGTACCAG CACGCCTTCA AGACCCCGGA TGCAGATGCC GGTGAAGAAA 
     4501 GAGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4561 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4621 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4681 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4741 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4801 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4861 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4921 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4981 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5041 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5101 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5161 CCTGTTGAAT TGGGTACGCG GCCAGCTTGG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA 
     5221 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5281 ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     5341 AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC 
     5401 AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG 
     5461 GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC 
     5521 TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA 
     5581 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG 
     5641 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC 
     5701 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT 
     5761 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT 
     5821 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC 
     5881 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC 
     5941 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC 
     6001 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG 
     6061 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG 
     6121 GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT 
     6181 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCC 
     6241 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA 
     6301 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC 
     6361 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC 
     6421 AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT 
     6481 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA 
     6541 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA 
     6601 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT 
     6661 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT 
     6721 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC 
     6781 ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC 
     6841 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC 
     6901 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG 
     6961 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 
     7021 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 
     7081 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 
     7141 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 
     7201 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 
     7261 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 
     7321 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 
     7381 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 
     7441 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 
     7501 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 
     7561 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 
     7621 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG 
     7681 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 
     7741 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 
     7801 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 
     7861 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA 
     7921 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA 
     7981 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC 
     8041 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA 
     8101 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG 
     8161 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG 
     8221 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG 
     8281 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG 
     8341 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC 
     8401 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT 
     8461 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA 
     8521 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA 
     8581 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA 
     8641 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC 
     8701 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC 
     8761 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 
     8821 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 
     8881 CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA 
     8941 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG 
     9001 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT 
     9061 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG 
     9121 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA 
     9181 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT 
     9241 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT 
     9301 TTAACGCGAA TTTT
//