LOCUS       MG214742                9227 bp    DNA     circular  SYN 
DEFINITION  Osbpl8 (tGFP-tagged) - Mouse oxysterol binding protein-like 8 (Osbpl8) transcript variant 2, (10ug)
ACCESSION   NM_001003717
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3708..4412 
                     /label="GFP"  
     misc_feature    4459..5044 
                     /label="PolyA signal"  
     misc_feature    7136..7781 
                     /label="ColE1"  
     misc_feature    5498..6292 
                     /label="Neo"  
     misc_feature    5129..5431 
                     /label="SV40 ori"  
     misc_feature    7796..8656 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3687..3693 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3687 
                     /label="Osbpl8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCAGCC TTAGCAGACG GAGAACCTGA CAGATCTTCG CTTCTTGGTG 
     1081 ATAGCAAAGA TGTCCTTGGG CCATCAACTG TTGTAGCGAA CAGTGACGAA CCTCAGCATT 
     1141 TGACCCCAGG AAAGATGAGT CAGCGCCAAG GCAGAGACGC TAACCCAACC CCAACTAGAG 
     1201 ATCTGCCTCA GCCTTCTCTC AGCCCCGCCA GCCTTCACAG CCAGGGTTTT GAAAGAGGAA 
     1261 AAGAAGACAT TTCTCAAAAT AAAGATGACT CTTCACTTTC AATGTCAAAG AGCAAGTCTG 
     1321 AATCTAAACT ATATAATGGC TCAGAAAAGG ACAGTTCAAC TTCAAGTAAA CTCACGAAGA 
     1381 AAGAGTCTCT TAAGGTACAA AAGAAAAACT ACCGTGAAGA GAAGAAGAGA GCCACGAAGG 
     1441 AGTTACTCAG CACCATCACA GACCCCTCTG TCATTGTCAT GGCCGACTGG CTGAAGATTC 
     1501 GTGGTACCCT GAAGAGCTGG ACCAAGCTGT GGTGTGTGTT GAAACCTGGC GTGCTTCTGA 
     1561 TCTATAAAAC ACAAAAAAAT GGTCAGTGGG TCGGAACAGT CCTTCTGAAT GCTTGTGAGA 
     1621 TCATTGAACG TCCATCAAAA AAGGATGGCT TTTGTTTCAA GCTTTTCCAT CCTTTGGAGC 
     1681 AGTCTATTTG GGCAGTGAAG GGTCCTAAAG GTGAAGCGGT TGGCTCCATA ACCCAGCCTC 
     1741 TACCTAGCAG CTACTTGATT ATCCGAGCTA CTTCAGAGTC AGATGGAAGG TGCTGGATGG 
     1801 ATGCTTTGGA GTTGGCTTTG AAGTGTTCCA GTCTCCTTAA GCGGACCATG GTGAGGGAGG 
     1861 GGAAGGAGCA CGACCTGAGC ATCTCCTCAG ACAGCACACA CGTGACGCTG TATGGCTTAC 
     1921 TGCGCGCTAA CAATCTCCAC AGTGGCGACA ACTTCCAGTT GAATGACAGT GAGATCGAAA 
     1981 GACAGCACTT TAAGGACCAA GATTTGTACT CTGATAAATC TGACAAGGAG AACGACCCGG 
     2041 AGCACGATGA GTCTGACAAC GAGGTGCTGG GGAAGAGCGA GGAGAGCGAC ACAGACACGT 
     2101 CCGAAAGGCA AGACGACTCG TACATCGACC CTGAGCCTGT GGAGCCCTTA AAGGAGACCA 
     2161 CCTACATGGA GCAGAGCCAC GAAGAACTGG GAGAGGCAGG TGAGGCCTCT CAAACGGAAA 
     2221 CTGTGTCTGA AGAAAACAAA AGCCTTATCT GGACGCTGCT CAAGCAAGTC CGTCCCGGCA 
     2281 TGGACCTATC CAGGGTGGTT CTTCCTACGT TTATTTTGGA GCCCCGCTCT TTCCTGGATA 
     2341 AGCTTTCAGA TTACTACTAC CACGCCGATT TCCTGTCTGA AGCTGCTCTA GAAGAAAATC 
     2401 CGTATTTCCG TTTGAAGAAA GTCGTGAAAT GGTATTTGTC AGGATTTTAT AAGAAGCCAA 
     2461 AGGGACTAAA GAAACCATAT AATCCTATAC TTGGTGAGAC TTTCCGATGT TTATGGATTC 
     2521 ATCCCAGAAC AAACAGCAAA ACTTTCTATA TTGCTGAACA GGTGTCCCAT CACCCACCAA 
     2581 TATCTGCCTT TTATGTCAGT AACCGGAAAG ATGGGTTTTG CCTTAGTGGT AGCATCCTGG 
     2641 CTAAGTCCAA GTTTTATGGA AACTCGTTAT CTGCCATATT GGAGGGAGAA GCCCGGTTAA 
     2701 CTTTCTTGAA CAGAGGTGAA GATTACGTCA TGACAATGCC ATATGCTCAC TGTAAAGGAA 
     2761 TTCTTTATGG CACAATGACC CTGGAGCTTG GTGGAACTGT CAATATTACC TGTCAGAAGA 
     2821 CTGGATACAG TGCGATACTT GAATTTAAAC TAAAGCCATT TCTAGGGAGT AGTGATTATG 
     2881 TTAACCAAAT ATCAGGGAAA CTTAAATTGG GAAAAGAAGT CCTAGCTACT TTGGAAGGCC 
     2941 ATTGGGATAG TGAAGTTTTC ATTAATGACA AAAAGACTGA TAACTCAGAG ATTTTCTGGA 
     3001 ATCCAACTCC TGACATTAAA CAGTGGAGAT TAATAAGGCA CACTGTAAAG TTTGAAGAAC 
     3061 AGGATGATTT CGAATCAGAG AAACTCTGGC AACGAGTGAC AAAAGCTATA AATGCCAAAG 
     3121 ACCAAACGGA AGCTACTCAA GAAAAGTATG TTCTGGAAGA AGCTCAAAGA CAAGCTGCCA 
     3181 GAGATCGAAA AACAAAAACT CAAGAGTGGG TTTGTAAGTT ATTTGAACTT GACCCACTCA 
     3241 CTGGAGAATG GCACTATAAG TTTTCAGATA CTCGACCATG GGACCCACTT AATGATATGA 
     3301 TACAATTTGA AAAAGATGGA GTTATCCAGA CCAAAGTGAA ACATCGCACT CCAATGGTTA 
     3361 GTGTTCCAAA AATGAAACAT AAACCAACAA GGCAGCAGAA GAAAGTGGTA AAAGGCTATT 
     3421 CTTCCCCAGA ACCTGACATC CAGGATTCCT CTGGAAGTGA AGCTCAATCT GTAAAACCAA 
     3481 GTACACGAAG GAAGAAGGGG ATAGACCTGG GAGACATTCA GAGTTCCATT GAGTCTATAA 
     3541 AGCAGACACA GGAAGAGATT AAAAGAAATA TTATGGCTCT TCGAAATCAT TTACTTTCAA 
     3601 GTACACCTGC TACAGATTAT TTTCTGCAAC AGAAAGACTA CTTCGTCATT TTCCTTCTGA 
     3661 TTTTGCTTCA AGTCATAATA AACTTTATAT TTAAGACGCG TACGCGGCCG CTCGAGATGG 
     3721 AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG CCGCATCACC GGCACCCTGA 
     3781 ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC CCCCGAGCAG GGCCGCATGA 
     3841 CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG CCCCTACCTG CTGAGCCACG 
     3901 TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG CGGCTACGAG AACCCCTTCC 
     3961 TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT CGAGAAGTAC GAGGACGGCG 
     4021 GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG CCGCGTGATC GGCGACTTCA 
     4081 AGGTGATGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT CACCGACAAG ATCATCCGCA 
     4141 GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA CGATCTGGAT GGCAGCTTCA 
     4201 CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTC CGTGGTGGAC AGCCACATGC 
     4261 ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG GGGCCCCATG TTCGCCTTCC 
     4321 GCCGCGTGGA GGAGGATCAC AGCAACACCG AGCTGGGCAT CGTGGAGTAC CAGCACGCCT 
     4381 TCAAGACCCC GGATGCAGAT GCCGGTGAAG AAAGAGTTTA AACGGCCGGC CGCGGTCATA 
     4441 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4501 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4561 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4621 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4681 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4741 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4801 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4861 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4921 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4981 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5041 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCT 
     5101 TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     5161 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 
     5221 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 
     5281 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 
     5341 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 
     5401 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 
     5461 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 
     5521 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 
     5581 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 
     5641 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 
     5701 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 
     5761 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 
     5821 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 
     5881 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 
     5941 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 
     6001 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 
     6061 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 
     6121 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCCGACCGCT ATCAGGACAT AGCGTTGGCT 
     6181 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 
     6241 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 
     6301 TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 
     6361 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 
     6421 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 
     6481 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 
     6541 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 
     6601 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 
     6661 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 
     6721 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 
     6781 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 
     6841 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 
     6901 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 
     6961 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 
     7021 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 
     7081 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 
     7141 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 
     7201 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 
     7261 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 
     7321 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 
     7381 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 
     7441 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 
     7501 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 
     7561 GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 
     7621 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 
     7681 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 
     7741 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 
     7801 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 
     7861 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 
     7921 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 
     7981 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 
     8041 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 
     8101 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 
     8161 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 
     8221 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 
     8281 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 
     8341 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 
     8401 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 
     8461 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 
     8521 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 
     8581 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 
     8641 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 
     8701 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 
     8761 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA 
     8821 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 
     8881 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 
     8941 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 
     9001 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 
     9061 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 
     9121 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 
     9181 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//