LOCUS       MG214539                9437 bp    DNA     circular  SYN 
DEFINITION  Map3k13 (tGFP-tagged) - Mouse mitogen-activated protein kinase kinase kinase 13 (Map3k13), (10ug)
ACCESSION   NM_172821
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3918..4622 
                     /label="GFP"  
     misc_feature    4669..5254 
                     /label="PolyA signal"  
     misc_feature    7346..7991 
                     /label="ColE1"  
     misc_feature    5708..6502 
                     /label="Neo"  
     misc_feature    5339..5641 
                     /label="SV40 ori"  
     misc_feature    8006..8866 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3897..3903 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3897 
                     /label="Map3k13"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAACCCT CAGGAGCACC TGAGCTGCTC CTCTTTGCCA CACTTGCCCT 
     1081 TGACTGAGAA CAAAACCTCT GGTGGACGAA ATGAGCTTGC AGCTATGGGG AACCACCCTT 
     1141 CACCCAAGCT CCCTGAGGAC CCTCAGGAGA GGGGGGCTAT ACAGTCAGAG CTGATGGAGA 
     1201 TCACAGGCAG CCCGATCTCC ACAACAGTGT TGACCAGTGT AAGCGAGGAT TCTAGGGGCC 
     1261 AGTTTGAGAA CAGTGTTCTT CAGCTAAGGG AACAGGATGA GTCAGAGATG ACCCTGTCTC 
     1321 TGGGGAACAG CAACACTGTG GATGGAGAGA ACACGAACGG ACCAGAAGAC ATCAAGATTC 
     1381 AGTTCAGCAG GTCAGGCAGC GGCAGCGGCG GGTTTCTCGA AGGACTGTTT GGATGTCTCA 
     1441 GGCCCGTGTG GAATATCATT GGGAAGGCAT ATTCCACTGA TTACAAGCTG CAGCAGCAAG 
     1501 ATACTTGGGA AGTGCCATTT GAGGAAATCT CAGAGCTGCA ATGGCTGGGT AGTGGAGCCC 
     1561 AAGGAGCTGT CTTCCTGGGC AAATTCCGAG CAGAGGAGGT GGCCATCAAG AAGGTGAGAG 
     1621 AACAAAATGA GACGGACATC AAGCATCTGA GGAAGCTGAA GCACCCTAAC ATCATCGCCT 
     1681 TCAAGGGTGT TTGCACTCAG GCCCCGTGCT ACTGCATCAT CATGGAGTAC TGTGCCCACG 
     1741 GACAGCTGTA CGAGGTCCTG AGAGCTGGCC GGAAGATCAC GCCCCGGCTG CTTGTGGACT 
     1801 GGTCCACGGG AATCGCCAGT GGAATGAATT ATCTCCATCT CCACAAGATT ATACACCGTG 
     1861 ACCTCAAGTC GCCCAATGTT TTAGTGACTC ACACAGATGC AGTGAAAATT TCAGACTTTG 
     1921 GCACATCTAA GGAACTCAGT GATAAAAGCA CCAAGATGTC CTTCGCTGGC ACCGTCGCGT 
     1981 GGATGGCACC AGAGGTGATC CGGAATGAGC CTGTCTCGGA GAAGGTGGAC ATATGGTCAT 
     2041 TTGGTGTGGT ACTTTGGGAG CTGCTGACAG GGGAGATTCC CTACAAGGAT GTGGACTCTT 
     2101 CCGCCATCAT TTGGGGTGTC GGCAGCAACA GCTTGCATCT CCCAGTTCCT TCCACTTGCC 
     2161 CGGATGGATT CAAAATCCTT ATGAAACAGA CATGGCAGAG TAAGCCTCGC AACCGACCTT 
     2221 CTTTCAGGCA GACGCTCATG CATCTGGATA TCGCGTCTGC AGATGTGCTC GCCACCCCTC 
     2281 AAGAAACTTA CTTCAAGTCT CAGGCTGAAT GGAGAGAAGA AGTGAAGAAA CATTTTGAGA 
     2341 AAATCAAAAG TGAAGGGACC TGTATACACC GATTAGATGA AGAACTGATT CGCCGGCGCA 
     2401 GGGAGGAGCT CAGGCATGCT CTGGATATTC GGGAGCACTA CGAGAGAAAG CTCGAACGGG 
     2461 CTAACAATCT GTACATGGAG CTGAGCGCCA TCATGCTGCA GCTGGAGATG CGCGAGAAGG 
     2521 AGCTCCTTAA GCGAGAGCAA GCAGTGGAAA AGAAGTACCC TGGAACCTAC AAACGACACC 
     2581 CTGTCCGTCC AATCATCCAC CCGAATGCCA TGGAGAAACT CATGAAAAGG AAGGGTGTGC 
     2641 CTCACAAGGC TGGGGTGCAG ACTAAGCGGC CAGACCTGTT GCGATCCGAA GGCATTCCTA 
     2701 GTACAGAAGC GGTTCCCACT GCATCCCCCC TGTCTGGAAG TCCCAAAATG TCCACCGCAA 
     2761 GCAGCAGAAG CCGGTACCGA AGCAAACCAC GTCACCGCCG AGGGAACAGT AGAGGCAGCC 
     2821 ATAGCGACTT CGCAGCAATC TTGAAAACCC AGCCCGCCCA GGAAAATTCA CCCCACCCCA 
     2881 CCTACATGCA CCACACTCAG GCTCAGTGCG CTTCAGTCCA TCAACATAAC CCTCTCCAGC 
     2941 AGCAATACCA GCAAATTCCT CCTGCCCAGC CTCAGAGCCG GCATCCCAGA CTCAATGCGC 
     3001 ATGGGCAGGA CATTGCAACC TGCGCCAATA ACCTGAGGTA CTTTGGCCCC GCAGCGGCCC 
     3061 TGCGGAGTCC ACTCAGCAAC CATGCACAAA GACAGATGCC TGGCTCAAGC CCCGACCTCA 
     3121 TCTCCACAGC CATGGCTGCA GATTGGAGAA ATTCTGAACT TGATCAAGAC CAAGTTGGAC 
     3181 CCTGGGGCTG TTGTCAGGCT GAACCCTATG ATCCCTGTTT CCAGTGCAGG CCAGAACATT 
     3241 CTGGGTCTTT AGATGTTCCC ACTACTGAGC CAGTGGGGAG GAGCCCAGAC CTTTCCAGTT 
     3301 CACCAGCACA CAATCCTCTT TCAGGAAATG CCCAAGGCTC TGAGAGAACA GGGGCAAATG 
     3361 GATTCAGTGG CTGTCAGTCT GGTATTTCTC ATCAATTCAC ACCCCCAATG CTACCTCAGA 
     3421 AGACACGGCC CCTTCAGAAG AGTGGCGATG ACTCCTCAGA AGAGGAAGGG GAAGTGGACA 
     3481 GTGAAGTAGA ATTTCCTCGA CGACAGAGGC CTCACCGCTG CATTAGCAGT TACCAGTCAT 
     3541 ACTCAACCTT TAGCTCCGAG AACTTCTCTG TGTCTGATGG AGAGGAGGGC AACACTAGTG 
     3601 ACCACTCGAA CAGTCCCGAT GAATCCGCCA ACAGACGGCA GGACCGCCTG GCAGAGACGC 
     3661 TGGACGACCT GCTGTCTCAG ACACCCGAGG CCCCCATCGA GATCTCCTCC CACTCAGATG 
     3721 GGCTGTCTGA CAAGGAGTGT GCTGTTCGGC GCGTGAAGAC ACAGATGTCC CTAGGCAAGC 
     3781 TGTGTGCCGA GGAGCGCGGC TACGAGAACC CTGTGCAGTT TGGAGACTCG GACTGTGACT 
     3841 CTTCAGAGGG AGAGTGTTCC GATGCCACAG TTAGGACCAG CAAGAACTAC AGCTCTGCCA 
     3901 CCTGGACGCG TACGCGGCCG CTCGAGATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG 
     3961 AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG 
     4021 GAGAGGGCAC CCCCGAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC 
     4081 TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA 
     4141 CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA 
     4201 ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT 
     4261 ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGATGGG CACCGGCTTC CCCGAGGACA 
     4321 GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA 
     4381 TGGGCGATAA CGATCTGGAT GGCAGCTTCA CCCGCACCTT CAGCCTGCGC GACGGCGGCT 
     4441 ACTACAGCTC CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC 
     4501 TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGGATCAC AGCAACACCG 
     4561 AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC GGATGCAGAT GCCGGTGAAG 
     4621 AAAGAGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4681 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4741 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4801 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4861 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4921 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4981 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5041 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5101 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5161 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5221 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5281 ATGCCTGTTG AATTGGGTAC GCGGCCAGCT TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT 
     5341 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5401 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5461 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 
     5521 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 
     5581 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 
     5641 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 
     5701 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 
     5761 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 
     5821 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 
     5881 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 
     5941 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 
     6001 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 
     6061 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 
     6121 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 
     6181 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 
     6241 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 
     6301 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 
     6361 GCCGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 
     6421 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 
     6481 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 
     6541 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 
     6601 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 
     6661 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 
     6721 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 
     6781 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 
     6841 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 
     6901 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 
     6961 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 
     7021 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 
     7081 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 
     7141 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 
     7201 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 
     7261 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 
     7321 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 
     7381 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 
     7441 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 
     7501 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 
     7561 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 
     7621 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 
     7681 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 
     7741 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT 
     7801 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 
     7861 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 
     7921 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 
     7981 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 
     8041 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 
     8101 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 
     8161 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 
     8221 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 
     8281 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 
     8341 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 
     8401 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 
     8461 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 
     8521 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 
     8581 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 
     8641 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 
     8701 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 
     8761 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 
     8821 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 
     8881 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     8941 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     9001 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 
     9061 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
     9121 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 
     9181 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 
     9241 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
     9301 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 
     9361 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
     9421 AATTTAACGC GAATTTT
//