LOCUS       MG214431                9389 bp    DNA     circular  SYN 
DEFINITION  Fam186b (tGFP-tagged) - Mouse family with sequence similarity 186 member B (Fam186b), (10ug)
ACCESSION   NM_001081254
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3870..4574 
                     /label="GFP"  
     misc_feature    4621..5206 
                     /label="PolyA signal"  
     misc_feature    7298..7943 
                     /label="ColE1"  
     misc_feature    5660..6454 
                     /label="Neo"  
     misc_feature    5291..5593 
                     /label="SV40 ori"  
     misc_feature    7958..8818 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3849..3855 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3849 
                     /label="Fam186b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAAGGAC AACCCCCCAC ACTTGGTGAC TCCCACATCA GTGAAGGCTA 
     1081 TTATCTCAAA GATTGAGACT GCCCAGCTAA TTCGGACTCA GGAGGATATT TCCACCCAGC 
     1141 TCTCGGACAT CTTGGACAAT GTCAACTGTG CCATCAACCG CTTCCAGGAA GAACTGGGAT 
     1201 ATGACTTAAA AGAAAGAGCG AAGCCTCACC AACCAGAGCA GAAAGGCAAG AAGAGGTTCA 
     1261 TCTTGCTGGA GAAACTCGCC GCCTTCTCTG ATGACGCCAA GGCCAAGGAG AAGCACCTGT 
     1321 ATGAGATCCT CCACTGGCTG GGCGAATGGG GTGACAGTCT GACTTATGAG ATAAAGGACA 
     1381 GGAGTTCGGA GGAAGACGAA GCCCTAGACG AATGGATCGA AGTGATGGAG AAGGTGTTGC 
     1441 CTCTTTCCCT CATGGCCACC AAAGGAGGCA TCGAGTCTCT CATCTCTCTG TGCTCGACTC 
     1501 TCATCGAAGA CCAGAAGAAA ATGACACAAA GGTACAAACA TAACTTCTGG CAGGACTGGC 
     1561 ATGAGAAGAG CCCACAGAAT AAGGAGCAGG GAATCCCTGT GCCACCGAGC CCGGAGCAGA 
     1621 TGCTCCAGGA CAAGAACACC GCCCACCTGA ACGTCTCGGA GGTCAGGTCC ATGCTACAGG 
     1681 AGCTCCTGGA CTCCACGATG TTCAACAAAG GGGAGGTTAA AGCCATCAGG TACATGTCCG 
     1741 CCGTGGTGGA GAACCTCAAC AAGGCCTTGA CCCTCCAGCA CAAGGAGAAC AAGAGTCTGG 
     1801 AGACAAAGTA CAAGTACCTG CAGGTGGAAA AGAACAAAGA GCTCAGCACC CAGAGGCTGC 
     1861 ACTTCCAAAA ATCCATCCAA GTCCTGGAGA GCAAAAGGGA CGCGCTGCTG AAACAGGTAA 
     1921 AGATTCTAGG GGACAAATAC CATCACCTCC TCATGTTAAA GCACACCCTG GAGTTCCAGC 
     1981 TGAATATAGC CCAGCCTGCT AGTGGTCCAG AAGAACCCAC CAAGCATTTC ATTCATTACC 
     2041 TGGAACCCCC TGAAAAAGAG CCCCTTCCAG GGCAGGGTAC AGCCCTGGAG AAGGAGAAGC 
     2101 AGGAGCCCAA GGAGAAAGGA CAGGCAGTTT CAGCTCTCTT TTCAAGTCCC CTGACCAAGG 
     2161 CCTGGCACAG TGGCACTACA CCTGCAGCCC CAAAGCCAAT CTTCGGCACG GACACGGATT 
     2221 CGAGGGTGAG GGATGTGTTC GTTGACCGCA CAGAGGCCCT GGAGCCTGTG CTGCTGCACT 
     2281 CAGAGGTCCC CCAGATTCCC ACACAATGGG AGAAACTAGG GGACATGAAA GACAAAGACC 
     2341 AGGGGGATCA CATCCAAGAG GAGGGAGCCC AAAGCAAGTC CCTGTCCCCA GGGAGCATCA 
     2401 GGAGGAGGCT TCTGGAGAGC CACATGGGGC ACTGGGAGGA AGAGCTCAAC TGGGAGACCA 
     2461 GAAGGCAGCA ATGGCTGCAG GAGGAAGAGA TGTGGCTTCA GCGGCAGAAG AAGTGGGCCC 
     2521 TGCTGGAGCA GGAGCACCAG GAAAAGGTGA GGCAGTGGGA GGCAGAGGCG GCTGCCAGGC 
     2581 AGCAGTGGCA GAGGCTCACC CAGCCAGAGG AGGAGAAGCC CAGCCCCAGG AAGGAGCAGA 
     2641 AGGCAAGCTC GGAGATGATC TTCACCACCA CCAGTCGGTG GAAGAGCCTG AAGAAGTCGG 
     2701 AGTCCACGCC GACGCCCCAC TGCAGGGCCC AGTCCGCGCA TCAGAGCAGG TCGTACATAC 
     2761 TCGCATCCCC ACACACCCAG CAGCCTGGGC AGGGGACCCA GAGGACCCTG GGTTCAGCTG 
     2821 AGTTTCTGCA AAGGCCACCG ACCCGCCAGG TTTCTGCGAA GCCCAAGAAA TCCACCTCCT 
     2881 TGCCAGTCAC TGGGACGTCC ATCCTCCGAA GGGTGTCCCA GGTCTCTCTG CAGCAAGCCC 
     2941 CGGGGACACC TAAGGACAAG GTCTACCACA TAGACATGGA GGCCCAGCTG AAGAACCTGC 
     3001 AGGTCCTGGG CGGTTCGGAG TCAGAGGTGG CACTGCCACA GTACCTGCGC AGCAAAGCCC 
     3061 TGGAGGTCAT TGCCATCGCC ATGGACCTGA GCATTCTCCG GCTGCAGTAC CTATGCAAGA 
     3121 AGTATATCCA ATACAGGCGC TTCCAGAGCC TCCGACTAGA AATGATCAAA CACATAGGAG 
     3181 CCATTCGACA AACAAGAGTT ACCAGTAAGG CCCAGAACCT CTATCTGTTC CTGGAGAACA 
     3241 TTGACCACCA GCAGAGCCTC AGGCTGCAGG CCTGGGCAGA CAAGCAGAGA GACCTGGAGG 
     3301 AAAGGCACCA GGAATGCCTG CACACCATGG TGGCCATGTT CCCTAAGCTC CAGCAAGAGT 
     3361 GGAACGTCCA CCTGAGCGTC CCCGTGCTCA CCCATGTGAA GCCAGTGAAG AGCAAGTCAT 
     3421 CTTCAGTCTT GCTTCAGAGA GTCCGCTCCA GCCACTCCAC CACCAAGCAC CGTCCTCCAC 
     3481 CTAAGCCCCG GGAGTCTGTG CCCCTGCCGA TAACTGGCCA ACAAGGGAGC CAGATGGAAG 
     3541 CCATATGGAA GGCTGATGTG GCCTCTTCGA GTCACCCCAT AGAAAAGAAG ACCCCTACAA 
     3601 ACATGTGCTG GGAACAACTA GGGGGCTACC CAGACATCCC CAGGTTACTG GTGGTGGACG 
     3661 AGCATCCCTC CTGCCCCAGA AGCTCGGTGT CCTTCAAGGC TCGCTCTTTC AGTGCTTCCT 
     3721 CGGTCCAAAG GAAGGAAGGC CAGGAACCCT CAGAGGAGCT AGCTGGGCCT GTCTGCAAAA 
     3781 TGTCCAGCCA GTCACTACCC GGATCGATCT GTACAAAGCA GGAAGGATCG GGCAGCTCCA 
     3841 GTACCCACTC CATCCCTACG CGTACGCGGC CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC 
     3901 TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC 
     3961 TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA 
     4021 CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT 
     4081 ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG 
     4141 GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT 
     4201 TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGATG GGCACCGGCT 
     4261 TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC 
     4321 ACCTGCACCC CATGGGCGAT AACGATCTGG ATGGCAGCTT CACCCGCACC TTCAGCCTGC 
     4381 GCGACGGCGG CTACTACAGC TCCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC 
     4441 ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGGATC 
     4501 ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCGGATGCAG 
     4561 ATGCCGGTGA AGAAAGAGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4621 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4681 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4741 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4801 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4861 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4921 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4981 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5041 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5101 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5161 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5221 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CTTGGCTGTG GAATGTGTGT 
     5281 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5341 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5401 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 
     5461 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 
     5521 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 
     5581 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 
     5641 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 
     5701 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 
     5761 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 
     5821 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 
     5881 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 
     5941 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 
     6001 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 
     6061 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 
     6121 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 
     6181 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 
     6241 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 
     6301 CGGCTGGGTG TGGCCGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 
     6361 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 
     6421 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 
     6481 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 
     6541 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 
     6601 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 
     6661 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 
     6721 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 
     6781 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 
     6841 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 
     6901 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 
     6961 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 
     7021 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 
     7081 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 
     7141 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 
     7201 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 
     7261 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 
     7321 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 
     7381 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 
     7441 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 
     7501 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 
     7561 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 
     7621 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 
     7681 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 
     7741 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 
     7801 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 
     7861 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 
     7921 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 
     7981 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 
     8041 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 
     8101 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 
     8161 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 
     8221 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 
     8281 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 
     8341 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 
     8401 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 
     8461 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 
     8521 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 
     8581 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 
     8641 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 
     8701 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 
     8761 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 
     8821 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 
     8881 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 
     8941 CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 
     9001 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 
     9061 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC 
     9121 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 
     9181 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 
     9241 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 
     9301 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC 
     9361 TGATTTAACA AAAATTTAAC GCGAATTTT
//