LOCUS       MG211201                9332 bp    DNA     circular  SYN 
DEFINITION  Lpin1 (tGFP-tagged) - Mouse lipin 1 (Lpin1), transcript variant 2
ACCESSION   NM_015763
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3813..4517 
                     /label="GFP"  
     misc_feature    4564..5149 
                     /label="PolyA signal"  
     misc_feature    7241..7886 
                     /label="ColE1"  
     misc_feature    5603..6397 
                     /label="Neo"  
     misc_feature    5234..5536 
                     /label="SV40 ori"  
     misc_feature    7901..8761 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3792..3798 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3792 
                     /label="Lpin1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATTACGTG GGGCAGCTGG CCGGCCAGGT GTTTGTGACG GTGAAGGAGC 
     1081 TCTACAAGGG ACTGAACCCC GCCACGCTGT CGGGATGCAT CGACATCATT GTCATCCGGC 
     1141 AGCCCAATGG CAGTCTGCAG TGCTCCCCTT TCCACGTCCG CTTCGGCAAG ATGGGTGTCC 
     1201 TCCGCTCCCG AGAGAAAGTG GTGGACATAG AAATCAATGG GGAGTCCGTG GATTTGCACA 
     1261 TGAAGTTGGG AGACAACGGA GAAGCATTTT TTGTTCAAGA GACTGACAAC GATCAGGAAA 
     1321 TCATCCCCAT GTACCTGGCC ACGTCCCCCA TCCTGTCAGA AGGAGCTGCG AGAATGGAAA 
     1381 GCCAGCTGAA GAGGAACTCT GTAGACAGAA TCAGGTGCCT GGATCCCACT ACAGCTGCCC 
     1441 AGGGCCTGCC TCCCAGCGAC ACCCCATCCA CTGGTTCTCT GGGGAAGAAG AGAAGGAAAA 
     1501 GGAGGAGGAA GGCCCAGTTG GACAATCTCA AAAGAGATGA CAATGTCAAC ACATCTGAGG 
     1561 ATGAGGACAT GTTTCCCATA GAGATGAGCT CGGATGAGGA CACAGCACCG ATGGATGGAA 
     1621 GCAGAACTCT TCCTAATGAT GTACCACCAT TCCAAGATGA TATTCCTAAG GAAAACTTCC 
     1681 CCTCGATTTC AACGTACCCC CAGTCAGCAT CGTATCCCAG TTCGGACAGA GAATGGTCCC 
     1741 CCAGCCCCAG CAGCCTGGTA GATTGCCAGA GGACTCCCCC TCACCTGGCC GAGGGAGTTC 
     1801 TCTCTAGCTC TTGTCCTCTG CAGTCTTGCC ACTTCCATGC TTCGGAAAGT CCTTCAGGCT 
     1861 CCCGGCCCTC AACACCAAAA AGTGACTCTG AGCTGGTCAG TAAGTCTGCA GACAGGTTGA 
     1921 CGCCAAAGAA TAACCTGGAA ATGCTCTGGC TGTGGGGTGA ATTGCCACAG GCTGCAAAGT 
     1981 CCTCTTCTCC ACACAAGATG AAAGAGTCCA GCCCCTTAGG GAGCCGGAAG ACTCCTGATA 
     2041 AAATGAATTT TCAGGCCATT CACAGCGAGT CTTCAGATAC TTTTAGTGAC CAGTCGCCAA 
     2101 CAATGGCCCG GGGACTGCTC ATCCACCAGA GTAAGGCCCA GACGGAAATG CAGTTTGTGA 
     2161 ACGAGGAGGA TCTCGAGTCC TTGGGGGCGG CAGCCCCACC TTCACCCGTG GCCGAAGAGC 
     2221 TCAAGGCCCC ATATCCCAAC ACCGCACAGT CGTCGAGCAA GACAGATTCC CCTTCCAGGA 
     2281 AGAAAGATAA ACGGAGCCGA CACCTTGGAG CTGATGGTGT TTATCTGGAC GACCTCACGG 
     2341 ACATGGACCC TGAAGTGGCA GCCCTGTATT TCCCCAAGAA TGGGGATCCT GGTGGGCTCC 
     2401 CCAAACAAGC CAGTGACAAC GGAGCCAGGT CAGCCAACCA GTCACCACAG TCTGTGGGAG 
     2461 GCTCGGGCAT CGACAGTGGT GTGGAGAGCA CCTCCGACAG CCTGAGGGAC CTGCCATCCA 
     2521 TCGCCATCTC CCTCTGCGGT GGCCTCAGTG ACCACAGAGA GATCACCAAA GATGCATTTT 
     2581 TGGAACAAGC CGTGTCATAT CAGCAATTTG CCGACAACCC TGCTATCATC GATGACCCCA 
     2641 ACCTCGTGGT CAAGGTTGGC AATAAGTATT ACAACTGGAC AACAGCAGCT CCTCTACTTC 
     2701 TGGCGATGCA GGCTTTCCAG AAACCTTTGC CAAAGGCCAC TGTGGAATCC ATCATGAGAG 
     2761 ATAAGATGCC CAAAAAGGGA GGAAGATGGT GGTTTTCCTG GAGAGGAAGA AATGCCACAA 
     2821 TCAAAGAGGA AAGCAAGCCT GAACAGTGCC TGACTGGGAA AGGCCACAAT ACCGGAGAGC 
     2881 AGCCTGCCCA GCTTGGCCTG GCCACCAGGA TAAAGCATGA GTCATCCTCC AGTGATGAAG 
     2941 AGCACGCAGC CGCCAAGCCA TCAGGTTCGA GCCACCTCTC TCTCTTGTCC AACGTCAGCT 
     3001 ACAAAAAGAC CCTGCGGCTC ACGTCGGAGC AGCTGAAAAG CTTGAAGTTG AAGAACGGCC 
     3061 CCAATGATGT GGTGTTCAGT GTCACTACCC AGTACCAGGG CACCTGTCGC TGCGAGGGCA 
     3121 CCATCTACCT GTGGAATTGG GACGACAAAG TCATCATCTC AGATATCGAT GGGACCATCA 
     3181 CAAGATCTGA TACTCTTGGT CACATTTTGC CCACGCTGGG AAAGGATTGG ACTCACCAGG 
     3241 GCATTGCAAA GCTGTACCAC AAAGTAAGCC AGAATGGCTA CAAGTTTCTC TATTGTTCGG 
     3301 CACGTGCCAT TGGGATGGCG GACATGACGA GGGGCTACCT GCACTGGGTC AACGAGAGGG 
     3361 GCACGGTGCT TCCACAGGGC CCGCTTCTGC TCAGCCCGAG CAGCCTCTTC TCCGCCTTGC 
     3421 ACAGAGAAGT GATTGAAAAG AAGCCAGAAA AGTTCAAAGT CCAGTGTTTG ACAGACATCA 
     3481 AGAACCTGTT TTTCCCAAAC ACAGAACCCT TCTATGCTGC TTTTGGGAAC CGGCCTGCTG 
     3541 ATGTGTATTC CTACAAGCAA GTGGGAGTGT CCCTGAATAG GATCTTCACT GTGAACCCCA 
     3601 AGGGTGAGCT GGTGCAGGAG CATGCCAAGA CCAACATCAG CTCGTACGTG CGGCTCTGCG 
     3661 AAGTGGTCGA TCACGTCTTC CCATTGCTAA AGAGAAGCCA TTCCTGTGAC TTCCCCTGTT 
     3721 CAGACACTTT CAGTAACTTC ACCTTTTGGA GAGAGCCACT GCCACCTTTT GAAAACCAGG 
     3781 ACATGCATTC AGCCTCAGCT ACGCGTACGC GGCCGCTCGA GATGGAGAGC GACGAGAGCG 
     3841 GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG 
     3901 AGCTGGTGGG CGGCGGAGAG GGCACCCCCG AGCAGGGCCG CATGACCAAC AAGATGAAGA 
     3961 GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT 
     4021 TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA 
     4081 ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA 
     4141 GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG ATGGGCACCG 
     4201 GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG 
     4261 AGCACCTGCA CCCCATGGGC GATAACGATC TGGATGGCAG CTTCACCCGC ACCTTCAGCC 
     4321 TGCGCGACGG CGGCTACTAC AGCTCCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA 
     4381 TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGG 
     4441 ATCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCGGATG 
     4501 CAGATGCCGG TGAAGAAAGA GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4561 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4621 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4681 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4741 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4801 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4861 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4921 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4981 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5041 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5101 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5161 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCTTGGCT GTGGAATGTG 
     5221 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5281 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5341 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 
     5401 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 
     5461 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 
     5521 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 
     5581 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 
     5641 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 
     5701 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 
     5761 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 
     5821 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 
     5881 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 
     5941 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 
     6001 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 
     6061 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 
     6121 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 
     6181 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 
     6241 GGCCGGCTGG GTGTGGCCGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 
     6301 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 
     6361 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 
     6421 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 
     6481 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 
     6541 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 
     6601 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 
     6661 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 
     6721 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
     6781 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 
     6841 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
     6901 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
     6961 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 
     7021 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     7081 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     7141 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     7201 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     7261 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     7321 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     7381 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     7441 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     7501 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     7561 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     7621 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     7681 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7741 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     7801 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 
     7861 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 
     7921 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 
     7981 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 
     8041 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 
     8101 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 
     8161 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 
     8221 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 
     8281 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 
     8341 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 
     8401 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 
     8461 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 
     8521 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 
     8581 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 
     8641 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 
     8701 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 
     8761 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     8821 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     8881 TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 
     8941 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 
     9001 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
     9061 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 
     9121 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 
     9181 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 
     9241 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 
     9301 AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//