LOCUS       MG211153                9302 bp    DNA     circular  SYN 
DEFINITION  Ano6 (tGFP-tagged) - Mouse transmembrane protein 16F (Tmem16f)
ACCESSION   NM_175344
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3781..4485 
                     /label="GFP"  
     misc_feature    4532..5117 
                     /label="PolyA signal"  
     misc_feature    7209..7854 
                     /label="ColE1"  
     misc_feature    5571..6365 
                     /label="Neo"  
     misc_feature    5202..5504 
                     /label="SV40 ori"  
     misc_feature    7869..8729 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3753..3760 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3753 
                     /label="Ano6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGATGATG ACTAGGAAGG TCCTGCTGAA CATGGAGCTG GAGGAGGACG 
     1081 ACGATGAGGA TGGAGACATT GTGCTGGAAA ACTTTGACCA GACAATTGTC TGCCCCACCT 
     1141 TTGGATCACT GGAGAATCAG CAGGACTTCA GGACTCCAGA GTTTGAAGAA TTTAACGGGA 
     1201 AGCCCGACTC CCTCTTTTTC ACCGATGGCC AGAGGCGAAT CGACTTCATC CTCGTGTATG 
     1261 AAGATGAGAG CAAAAAGGAG AACAATAAGA AAGGGACAAA TGAGAAACAG AAGAGGAAAA 
     1321 GACAAGCATA CGAATCTAAC CTTATCTGCC ATGGGCTGCA GCTGGAAGCA ACAAGATCTG 
     1381 TTTCTGATGA CAAGCTTGTG TTCGTAAAAG TGCACGCGCC CTGGGAAGTG CTGTGCACCT 
     1441 ATGCTGAGAT CATGCACATC AAACTCCCGC TAAAGCCAAA CGACCTGAAA ACGCGCTCGC 
     1501 CCTTTGGCAA CCTCAACTGG TTCACCAAGG TCCTCCGGGT GAACGAGAGT GTCATCAAGC 
     1561 CAGAGCAGGA GTTCTTCACT GCCCCTTTTG AGAAGAGCCG GATGAATGAT TTCTACATCC 
     1621 TCGATAGAGA TTCCTTCTTC AACCCTGCCA CCAGAAGCCG CATTGTTTAT TTCATCCTCT 
     1681 CTCGGGTCAA ATACCAAGTG ATGAACAACG TTAACAAATT TGGGATTAAT AGACTGGTCA 
     1741 GCTCTGGAAT CTACAAAGCA GCGTTTCCTC TGCACGACTG CAGATTCAAC TATGAGTCGG 
     1801 AGGACATCAG TTGTCCTAGC GAGCGTTACC TCCTGTACAG AGAATGGGCT CACCCTCGGA 
     1861 GTATATACAA GAAGCAGCCC TTGGATCTTA TCAGGAAGTA TTACGGCGAG AAGATTGGAA 
     1921 TCTACTTTGC TTGGCTGGGC TATTACACGC AGATGCTCCT TCTCGCAGCT GTGGTGGGCG 
     1981 TGGCCTGCTT CCTCTATGGA TATCTTGATC AGGATAACTG CACTTGGAGC AAAGAGGTCT 
     2041 GTGATCCTGA CATCGGTGGC CAGATCCTGA TGTGCCCCCA GTGTGACAGG CTGTGTCCGT 
     2101 TCTGGAGGCT GAATATCACC TGTGAATCTT CTAAGAAATT GTGCATCTTT GACAGTTTTG 
     2161 GAACCCTGAT CTTCGCTGTA TTTATGGGAG TGTGGGTTAC ATTGTTTTTG GAGTTTTGGA 
     2221 AGCGGCGCCA GGCAGAGCTC GAATATGAAT GGGACACCGT TGAGCTACAG CAAGAGGAAC 
     2281 AGGCCCGGCC AGAGTATGAG GCCCAGTGCA ATCACGTGGT GATAAACGAG ATCACTCAGG 
     2341 AGGAGGAGCG CATCCCATTT ACCACCTGTG GGAAGTGCAT CCGGGTCACG CTGTGTGCGA 
     2401 GCGCCGTCTT CTTCTGGATC CTGCTCATCA TCGCCTCTGT GATCGGGATC ATTGTCTACA 
     2461 GGCTGTCCGT GTTCATCGTA TTTTCCACAA CGCTCCCCAA GAACCCCAAC GGGACAGACC 
     2521 CGATCCAGAA GTACCTGACC CCACAGATGG CCACATCCAT CACAGCCTCC ATCATCAGCT 
     2581 TCATCATCAT CATGATCCTC AACACGATCT ACGAGAAGGT GGCCATCATG ATCACCAACT 
     2641 TCGAGCTCCC AAGGACCCAG ACGGATTATG AGAACAGCCT GACCATGAAG ATGTTCTTGT 
     2701 TCCAGTTTGT CAACTATTAC TCCTCATGCT TCTACATCGC ATTCTTCAAG GGCAAATTCG 
     2761 TGGGCTATCC CGGGGACCCA GTGTACTTGC TGGGCAAATA CAGAAGCGAA GAGTGTGACC 
     2821 CGGGGGGCTG CCTCCTTGAA CTGACCACAC AGCTGACGAT CATCATGGGG GGAAAGGCAA 
     2881 TCTGGAACAA CATACAAGAA GTCTTGCTCC CATGGGTTAT GAATCTAATT GGACGATATA 
     2941 AAAGAGTCTC GGGATCAGAA AAGATAACCC CACGATGGGA ACAGGATTAC CATCTGCAGC 
     3001 CCATGGGCAA GCTGGGATTG TTCTATGAGT ATCTTGAAAT GATTATTCAG TTTGGGTTCG 
     3061 TCACTTTATT TGTGGCCTCT TTCCCACTGG CTCCCCTGCT GGCTCTGGTG AACAATATTT 
     3121 TGGAAATAAG AGTGGACGCG TGGAAGCTCA CAACCCAGTT TAGGCGCATG GTGCCCGAAA 
     3181 AAGCCCAGGA TATCGGCGCG TGGCAGCCCA TCATGCAAGG AATAGCCATC CTGGCCGTGG 
     3241 TGACCAACGC CATGATCATT GCTTTCACAT CAGACATGAT CCCCCGCCTG GTGTATTATT 
     3301 GGTCTTTCTC TATCCCTCCC TACGGGGACC ACACTTATTA CACCATGGAC GGCTACATCA 
     3361 ACAACACTCT CTCTGTCTTC AACATCACGG ACTTCAAGAA CACAGACAAA GAAAACCCAT 
     3421 ACATTGGGCT TGGTAACTAT ACCTTGTGCA GGTACCGTGA CTTCCGAAAC CCACCTGGGC 
     3481 ACCCACAAGA GTATAAACAC AACATCTACT ATTGGCACGT GATTGCCGCC AAGCTGGCCT 
     3541 TCATCATTGT CATGGAGCAC ATCATCTACT CTGTGAAGTT CTTCATTTCC TACGCAATTC 
     3601 CAGATGTGTC CAAAATCACG AAAAGCAAGA TCAAGAGGGA GAAATACCTC ACGCAGAAGT 
     3661 TGCTTCACGA GAGTCACCTC AAAGACCTGA CCAAAAACAT GGGGATCATC GCCGAGAGGA 
     3721 TAGGAGGAAC GGTTGACAAC AGCGTGCGGC CAAAACTCGA AAGCGGACCG ACGCGTACGC 
     3781 GGCCGCTCGA GATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA 
     3841 TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCG 
     3901 AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT 
     3961 ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT 
     4021 ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA 
     4081 AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG 
     4141 TGATCGGCGA CTTCAAGGTG ATGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG 
     4201 ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGATC 
     4261 TGGATGGCAG CTTCACCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTCCGTGG 
     4321 TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC 
     4381 CCATGTTCGC CTTCCGCCGC GTGGAGGAGG ATCACAGCAA CACCGAGCTG GGCATCGTGG 
     4441 AGTACCAGCA CGCCTTCAAG ACCCCGGATG CAGATGCCGG TGAAGAAAGA GTTTAAACGG 
     4501 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4561 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4621 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4681 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4741 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4801 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4861 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4921 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4981 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5041 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5101 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5161 GGTACGCGGC CAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 
     5221 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 
     5281 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5341 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 
     5401 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 
     5461 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 
     5521 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 
     5581 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 
     5641 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 
     5701 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 
     5761 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 
     5821 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 
     5881 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 
     5941 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 
     6001 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 
     6061 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 
     6121 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 
     6181 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCCGA CCGCTATCAG 
     6241 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 
     6301 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 
     6361 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA 
     6421 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 
     6481 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 
     6541 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 
     6601 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 
     6661 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 
     6721 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
     6781 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 
     6841 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 
     6901 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 
     6961 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 
     7021 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
     7081 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     7141 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     7201 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     7261 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     7321 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     7381 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     7441 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     7501 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     7561 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     7621 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC 
     7681 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 
     7741 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 
     7801 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 
     7861 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 
     7921 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 
     7981 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 
     8041 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 
     8101 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 
     8161 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 
     8221 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 
     8281 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 
     8341 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 
     8401 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 
     8461 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 
     8521 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 
     8581 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 
     8641 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 
     8701 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 
     8761 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 
     8821 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT 
     8881 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 
     8941 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 
     9001 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 
     9061 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 
     9121 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 
     9181 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 
     9241 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 
     9301 TT
//