LOCUS       MG210911                9077 bp    DNA     circular  SYN 
DEFINITION  Vps16 (tGFP-tagged) - Mouse vacuolar protein sorting 16 (yeast) (Vps16)
ACCESSION   NM_030559
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3558..4262 
                     /label="GFP"  
     misc_feature    4309..4894 
                     /label="PolyA signal"  
     misc_feature    6986..7631 
                     /label="ColE1"  
     misc_feature    5348..6142 
                     /label="Neo"  
     misc_feature    4979..5281 
                     /label="SV40 ori"  
     misc_feature    7646..8506 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3537..3543 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3537 
                     /label="Vps16"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTGTTAC ACTGCGAACT GGAATCCACT CGGGGACTCT GCCTTTTACA 
     1081 GGAAGTATGA GCTGTACAGC ATGGACTGGG ACCTGAAGGA GGAGCTCAAG GATTGCCTGG 
     1141 TGGCTGCTGC ACCCTATGGG GGTCCCATTG CTCTACTGAG GAACTGTTGG AGAAAAGAGA 
     1201 AAGCTGCCAG CGTGCGGCCA GTACTGGAGA TCTACTCTGC ATCTGGCTTA CCACTGGCTA 
     1261 GCCTGCTGTG GAAGAGTGGG CCTGTGGTGG CCCTCGGCTG GTCAGCTGAG GAGGAGCTGC 
     1321 TCTGTGTGCA AGAAGATGGT GCAGTGCTAG TTTATGGCCT TCATGGAGAC TTCCGGAGGC 
     1381 ACTTCAGCAT GGGCAATGAA GTGCTTCAGA ACCGTGTCCT GGATGCCCGG ATTTTTCACA 
     1441 CTGAGTTTGG TTCAGGGGTG GCCATCCTTA CAGGAGCCTA TCGCTTTACT CTGAGTGCCA 
     1501 ATGTGGGTGA CCTCAAACTC CGTCGGATGC CGGAGGTGCC AGGTATGCAA AGTGCACCCT 
     1561 CATGTTGGAC CACACTGTGC CACGACCGAG TACCACACAT TCTTCTGGCT GTAGGACCCG 
     1621 ATCTTTACCT TCTGGATCAT GCTACCTGCT CTGCAGTGAC ACCTGCTGGC TTAGCCCCAG 
     1681 GAGTGAGCAG CTTCCTACAG ATGGCTGTAT CCTTCACGTA TCGTTACCTG GCACTCTTCA 
     1741 CAGACACAGG CTACATCTGG ATGGGGACAG CATCACTCAA GGAGAAGCTG TGTGAGTTCA 
     1801 ACTGCAACAT CCGGGCTCCC CCGAAGCAGA TGGTCTGGTG TAGCCGTCCT CGGAGCAAGG 
     1861 AAAGGGCTGT TGTTGTGGCC TGGGAGAGGC GGCTGATGGT GGTGGGCAAT GCGCCTGAAA 
     1921 GCATCCAGTT TGTGCTCGAT GAGGACTCCT ACTTAGTGCC TGAGCTTGAT GGGGTCCGCA 
     1981 TCTTCTCCCG AAGCACCCAT GAGTTCCTGC ATGAAGTCCC AGTGGCCAGT GAAGAGATCT 
     2041 TCAAAATTGC CTCAATGGCC CCTGGAGCAC TGCTGTTAGA GGCCCAGAAG GAATATGAGA 
     2101 AAGAGAGCCA GAAAGCAGAT GAGTACCTGC GGGAGATCCA GGAACTGGGC CAGCTGATCC 
     2161 AGGCTGTGCA GCAGTGCATT GAGGCTGCAG GACATGAGCA CCAGCCAGAC ATGCAAAAGA 
     2221 GTCTGCTCAG GGCGGCTTCA TTTGGAAAGT GTTTCCTTGA CAGATTTCCA CCTGACAGCT 
     2281 TCGTGCACAT GTGTCAGGAC CTTCGAGTGC TCAATGCTAT TAGAGACTAC CACATTGGGA 
     2341 TCCCTCTCAC CTACACCCAA TACAAGCAGC TCACCATCCA GGTGCTGCTA GACAGGCTTG 
     2401 TGTTGCGGAG GCTTTATCCC CTGGCTATCC AGATATGCGA GTACCTGCGC CTTCCTGAAG 
     2461 TCCAGGGTGT CAGCAGGATC CTAGCCCACT GGGCCTGCTA CAAGGTGCAG CAGAAAGATG 
     2521 TCTCAGATGA AGATGTAGCA CGGGCTATTA ATCAGAAGCT GGGTGACACG CCTGGTGTTT 
     2581 CATACTCTGA CATTGCTGCG AGGGCCTATG GCTGTGGCCG CACTGAACTG GCCATTAAGC 
     2641 TGCTGGAGTA TGAGCCACGC TCAGGGGAGC AGGTACCTCT TCTCCTAAAG ATGAAGAGGA 
     2701 GCAAACTGGC ACTGAGCAAG GCTATTGAGA GTGGAGACAC TGACCTGGTG TTTACAGTGT 
     2761 TGCTGCACCT AAAGAATGAA CTCAACCGAG GAGACTTTTT CATGACTCTC CGGAACCAAC 
     2821 CCATGGCCCT GAGTTTGTAT CGACAGTTCT GTAAGCATCA GGAGCTAGAC ACTCTGAAGG 
     2881 ACCTTTACAA TCAGGACGAC AATCACCAGG AGTTGGGCAG CTTCCACATC AGAGCCAGCT 
     2941 ATGCCGCTGA AGAGCGTATT GAAGGGCGAG TGGCAGCTCT GCAGATGGCA GCTGATGCCT 
     3001 TCTACAAAGC CAAGAATGAA TTTGCAGCCA AGGCCACAGA GGATCAAATG AGGCTCCTGC 
     3061 GGATACAGCG ACGCCTAGAG GATGAGCTAG GGGGCAGGTT CCTAGACCTA TCTCTACACG 
     3121 ACACTGTCAC CACTCTTATC CTTGGAGGCC ACAACAAGCG GGCAGAGCAG CTGGCACGTG 
     3181 ACTTTCGCAT CCCTGATAAG AGACTATGGT GGTTAAAGCT GGCTGCCCTG GCAGATTTGG 
     3241 AAGATTGGGA GGAGCTGGAG AAGTTTTCCA AAAGCAAGAA ATCACCGATT GGTTACCTGC 
     3301 CCTTTGTAGA GATCTGCATG AAACAGCATA ACAAACATGA GGCCAAGAAG TATGCTTCCC 
     3361 GTGTGGGCCC TGAACAAAAG GTCAAGGCTT TGCTTCTTGT TGGGGATGTG GCTCAGGCTG 
     3421 CAGAGGTGGC TATTGAGCAC CGGAATGAAA CAGAGCTGAG CCTCGTTTTG TCCCACTGCA 
     3481 CTGGAGCCAC AGATGGGGCC ATAGCTGACA AGATTCAGCG GGCCAGGGCA CAAGCCCAGA 
     3541 AGAAGACGCG TACGCGGCCG CTCGAGATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG 
     3601 AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG 
     3661 GAGAGGGCAC CCCCGAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC 
     3721 TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA 
     3781 CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA 
     3841 ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT 
     3901 ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGATGGG CACCGGCTTC CCCGAGGACA 
     3961 GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA 
     4021 TGGGCGATAA CGATCTGGAT GGCAGCTTCA CCCGCACCTT CAGCCTGCGC GACGGCGGCT 
     4081 ACTACAGCTC CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC 
     4141 TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGGATCAC AGCAACACCG 
     4201 AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC GGATGCAGAT GCCGGTGAAG 
     4261 AAAGAGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4321 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4381 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4441 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4501 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4561 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4621 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4681 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4741 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4801 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4861 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4921 ATGCCTGTTG AATTGGGTAC GCGGCCAGCT TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT 
     4981 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5041 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5101 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 
     5161 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 
     5221 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 
     5281 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 
     5341 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 
     5401 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 
     5461 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 
     5521 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 
     5581 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 
     5641 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 
     5701 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 
     5761 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 
     5821 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 
     5881 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 
     5941 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 
     6001 GCCGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 
     6061 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 
     6121 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 
     6181 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 
     6241 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 
     6301 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 
     6361 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 
     6421 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 
     6481 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 
     6541 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 
     6601 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 
     6661 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 
     6721 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 
     6781 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 
     6841 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 
     6901 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 
     6961 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 
     7021 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 
     7081 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 
     7141 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 
     7201 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 
     7261 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 
     7321 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 
     7381 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT 
     7441 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 
     7501 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 
     7561 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 
     7621 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 
     7681 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 
     7741 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 
     7801 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 
     7861 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 
     7921 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 
     7981 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 
     8041 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 
     8101 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 
     8161 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 
     8221 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 
     8281 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 
     8341 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 
     8401 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 
     8461 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 
     8521 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     8581 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     8641 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 
     8701 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
     8761 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 
     8821 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 
     8881 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
     8941 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 
     9001 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
     9061 AATTTAACGC GAATTTT
//