LOCUS       MG210883                9062 bp    DNA     circular  SYN 
DEFINITION  Trim28 (tGFP-tagged) - Mouse tripartite motif protein 28 (Trim28)
ACCESSION   NM_011588
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3543..4247 
                     /label="GFP"  
     misc_feature    4294..4879 
                     /label="PolyA signal"  
     misc_feature    6971..7616 
                     /label="ColE1"  
     misc_feature    5333..6127 
                     /label="Neo"  
     misc_feature    4964..5266 
                     /label="SV40 ori"  
     misc_feature    7631..8491 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3522..3528 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3522 
                     /label="Trim28"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCCTCG GCGGCAGCGA CTGCAGCGGC CTCGGCCGCG ACGGCCGCCT 
     1081 CGGCGGCCTC TGGTAGCCCA GGGTCGGGCG AGGGCTCGGC GGGCGGTGAG AAGCGTCCGG 
     1141 CTGCTTCCTC AGCCGCGGCG GCCTCTGCAG CCGCGTCGTC CCCTGCGGGG GGCGGTGGCG 
     1201 AGGCGCAGGA GCTTCTGGAG CACTGCGGCG TGTGTCGCGA GCGCCTGCGG CCCGAGCGGG 
     1261 ATCCTCGGCT GCTGCCCTGT CTACATTCGG CCTGCAGTGC CTGCCTGGGC CCCGCTACAC 
     1321 CCGCCGCAGC GAATAATTCG GGGGATGGCG GCTCGGCGGG CGACGGCGCT ATGGTGGATT 
     1381 GTCCAGTGTG CAAACAGCAG TGCTACTCCA AAGACATCGT GGAGAATTAT TTTATGCGTG 
     1441 ATAGTGGCAG TAAGGCCTCT TCTGATTCCC AGGATGCTAA CCAGTGCTGC ACTAGCTGTG 
     1501 AAGATAATGC CCCAGCCACT AGCTATTGTG TGGAGTGCTC TGAACCACTT TGTGAGACCT 
     1561 GTGTGGAGGC TCACCAGCGG GTGAAATACA CCAAGGACCA CACTGTGCGC TCCACAGGAC 
     1621 CTGCTAAGAC TCGAGATGGA GAGCGAACAG TCTACTGTAA TGTGCACAAG CATGAGCCCC 
     1681 TCGTGCTGTT CTGTGAGAGC TGTGACACAC TCACCTGCCG CGACTGCCAG CTCAACGCTC 
     1741 ACAAGGACCA TCAGTACCAG TTTTTGGAAG ATGCAGTGAG GAACCAACGT AAACTCTTGG 
     1801 CTTCACTGGT GAAACGTCTT GGGGACAAAC ATGCCACACT TCAGAAAAAC ACCAAGGAGG 
     1861 TTCGAAGCTC GATCCGCCAG GTGTCTGATG TGCAGAAGCG AGTGCAGGTT GATGTCAAGA 
     1921 TGGCCATTCT GCAGATCATG AAGGAGCTGA ATAAGCGGGG TCGAGTTCTG GTCAATGATG 
     1981 CCCAGAAGGT GACCGAGGGT CAGCAGGAAC GTCTGGAGCG CCAGCACTGG ACCATGACCA 
     2041 AAATTCAGAA GCACCAGGAA CACATTTTGC GTTTTGCCTC TTGGGCTCTG GAGAGTGATA 
     2101 ACAATACAGC TCTCTTGCTC TCTAAGAAGC TGATCTATTT CCAGCTGCAT CGGGCCCTCA 
     2161 AAATGATTGT GGATCCTGTG GAGCCTCATG GTGAGATGAA GTTTCAGTGG GATCTCAATG 
     2221 CCTGGACCAA GAGTGCTGAA GCCTTTGGCA AGATTGTGGC TGAGCGTCCT GGTACGAACT 
     2281 CCACAGGTCC TGGGCCCATG GCTCCTCCAA GAGCCCCAGG CCCTCTAAGC AAGCAAGGTT 
     2341 CTGGCAGTAG CCAGCCCATG GAAGTACAAG AGGGATATGG CTTTGGGTCA GATGATCCCT 
     2401 ATTCAAGTGC AGAGCCGCAT GTATCAGGCA TGAAGCGGTC CCGCTCTGGT GAGGGAGAGG 
     2461 TAAGTGGCCT CTTAAGGAAG GTGCCACGTG TGAGCCTTGA ACGCCTGGAT CTGGACCTCA 
     2521 CCTCTGACAG CCAGCCACCA GTCTTCAAGG TCTTTCCTGG AAGCACTACT GAGGACTACA 
     2581 ATCTGATTGT TATTGAGCGT GGTGCTGCTG CAGCAGCTGC TGGTCAGGCT GGGACTGTGC 
     2641 CACCAGGAGC CCCTGGTGCC CCACCCCTTC CTGGCATGGC CATTGTCAAG GAAGAAGAGA 
     2701 CAGAAGCTGC TATTGGAGCT CCCCCGGCTG CCCCCGAGGG TCCTGAAACC AAGCCTGTGT 
     2761 TGATGCCTCT GACTGAAGGT CCTGGTGCCG AGGGACCTCG TCTAGCTTCA CCTAGTGGCA 
     2821 GTACCAGCTC AGGCTTGGAG GTGGTGGCTC CTGAGGTTAC CTCAGCCCCA GTAAGTGGGC 
     2881 CAGGTATCCT GGATGACAGT GCCACTATCT GCCGTGTCTG CCAGAAACCA GGTGACCTGG 
     2941 TCATGTGTAA CCAGTGCGAA TTTTGCTTCC ACCTGGATTG CCACCTCCCT GCCCTGCAGG 
     3001 ATGTTCCAGG GGAGGAATGG AGTTGCTCAC TCTGCCACGT GCTCCCTGAC CTAAAGGAGG 
     3061 AAGATGGAAG CCTCAGCCTG GATGGAGCAG ATAGCACTGG TGTGGTAGCT AAACTCTCAC 
     3121 CAGCCAACCA GCGGAAATGT GAGCGTGTTC TCCTGGCCCT GTTCTGCCAT GAACCATGCC 
     3181 GTCCCTTGCA TCAGCTGGCT ACCGACTCTA CATTCTCCAT GGAGCAGCCT GGTGGTACCC 
     3241 TAGACCTGAC CTTGATTCGT GCTCGCCTCC AAGAGAAGCT GTCACCTCCT TATAGCTCCC 
     3301 CCCAGGAGTT TGCTCAAGAT GTGGGCCGCA TGTTCAAACA GTTCAACAAG CTGACTGAGG 
     3361 ACAAGGCAGA TGTTCAGTCC ATCATCGGCT TGCAGCGCTT CTTTGAGACA CGCATGAATG 
     3421 ATGCCTTTGG TGACACCAAG TTTTCTGCTG TGCTGGTAGA ACCACCACCA TTGAACCTTC 
     3481 CCAGTGCTGG CCTAAGTTCT CAGGAGCTCT CTGGCCCTGG TGATGGCCCC ACGCGTACGC 
     3541 GGCCGCTCGA GATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA 
     3601 TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCG 
     3661 AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT 
     3721 ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT 
     3781 ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA 
     3841 AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG 
     3901 TGATCGGCGA CTTCAAGGTG ATGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG 
     3961 ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGATC 
     4021 TGGATGGCAG CTTCACCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTCCGTGG 
     4081 TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC 
     4141 CCATGTTCGC CTTCCGCCGC GTGGAGGAGG ATCACAGCAA CACCGAGCTG GGCATCGTGG 
     4201 AGTACCAGCA CGCCTTCAAG ACCCCGGATG CAGATGCCGG TGAAGAAAGA GTTTAAACGG 
     4261 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4321 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4381 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4441 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4501 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4561 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4621 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4681 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4741 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4801 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4861 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4921 GGTACGCGGC CAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 
     4981 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 
     5041 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5101 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 
     5161 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 
     5221 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 
     5281 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 
     5341 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 
     5401 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 
     5461 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 
     5521 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 
     5581 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 
     5641 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 
     5701 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 
     5761 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 
     5821 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 
     5881 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 
     5941 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCCGA CCGCTATCAG 
     6001 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 
     6061 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 
     6121 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA 
     6181 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 
     6241 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 
     6301 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 
     6361 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 
     6421 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 
     6481 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
     6541 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 
     6601 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 
     6661 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 
     6721 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 
     6781 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
     6841 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     6901 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     6961 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     7021 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     7081 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     7141 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     7201 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     7261 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     7321 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     7381 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC 
     7441 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 
     7501 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 
     7561 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 
     7621 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 
     7681 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 
     7741 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 
     7801 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 
     7861 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 
     7921 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 
     7981 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 
     8041 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 
     8101 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 
     8161 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 
     8221 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 
     8281 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 
     8341 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 
     8401 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 
     8461 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 
     8521 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 
     8581 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT 
     8641 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 
     8701 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 
     8761 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 
     8821 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 
     8881 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 
     8941 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 
     9001 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 
     9061 TT
//