LOCUS       MG210420                8793 bp    DNA     circular  SYN 
DEFINITION  Col8a1 (tGFP-tagged) - Mouse procollagen, type VIII, alpha 1 (Col8a1)
ACCESSION   NM_007739
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3274..3978 
                     /label="GFP"  
     misc_feature    4025..4610 
                     /label="PolyA signal"  
     misc_feature    6702..7347 
                     /label="ColE1"  
     misc_feature    5064..5858 
                     /label="Neo"  
     misc_feature    4695..4997 
                     /label="SV40 ori"  
     misc_feature    7362..8222 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3253..3259 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3253 
                     /label="Col8a1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCACTGCCA CCCAGGCCTC TACAGCTGCT GGGAATACTG TTCATCATTT 
     1081 CCCTGAACTC TGTCAGACTC ATTCAGGCCG GTGCCTACTA TGGAATCAAG CCTCTGCCAC 
     1141 CTCAAATCCC TCCTCAGATA CCACCACAAA TTCCACAGTA CCAGCCCTTG GGCCAGCAAG 
     1201 TCCCTCACAT GCCTTTGGGC AAAGATGGCC TTTCCATGGG CAAGGAGATG CCTCACATGC 
     1261 AGTATGGCAA AGAGTACCCA CACCTACCCC AATATATGAA GGAAATCCCA CCTGTGCCAA 
     1321 GAATGGGCAA GGAAGTGGTG CCCAAAAAAG GCAAAGGAGA AGTACCGTTA GCCAGTTTGC 
     1381 GCGGAGAACA AGGTCCCCGT GGAGAACCTG GACCAAGAGG ACCACCTGGG CCGCCAGGTT 
     1441 TACCAGGTCA TGGAATGCCT GGAATCAAAG GAAAACCAGG GCCCCAGGGA TATCCAGGAA 
     1501 TTGGAAAGCC CGGTATGCCT GGAATGCCTG GGAAGCCAGG AGCCATGGGA ATGCCAGGGG 
     1561 CAAAAGGCGA AATTGGACCC AAAGGGGAAA TCGGACCTAT GGGAATCCCA GGGCCACAAG 
     1621 GGCCTCCAGG ACCTCATGGA CTTCCTGGCA TCGGGAAACC GGGTGGGCCA GGGTTACCAG 
     1681 GGCAACCAGG AGCAAAAGGT GAGAGGGGGC CCAAAGGACC ACCAGGACCT CCCGGCCTTC 
     1741 AGGGTCCCAA AGGAGAGAAG GGCTTTGGGA TGCCTGGCTT GCCAGGTCTG AAGGGTCCTC 
     1801 CAGGTATGCA TGGCCCTCCT GGACCAGTTG GACTACCGGG AGTAGGAAAA CCAGGAGTGA 
     1861 CAGGCTTTCC TGGACCACAG GGTCCCCTGG GGAAGCCAGG GCCTCCAGGG GAACCTGGAC 
     1921 CACAAGGCCT TATTGGTGTG CCAGGAGTTC AAGGACCTCC TGGGATGCCT GGAGTTGGAA 
     1981 AGCCAGGTCA GGATGGGATC CCGGGCCAAC CAGGATTTCC GGGTGGTAAA GGGGAACAAG 
     2041 GATTGCCAGG GTTGCCTGGA CCCCCAGGCC TCCCAGGGGT CGGAAAGCCA GGTTTCCCAG 
     2101 GACCCAAAGG GGACCGGGGT ATTGGGGGTG TTCCTGGGGT TCTTGGGCCG AGAGGGGAAA 
     2161 AAGGACCCAT TGGTGCTCCT GGAATGGGGG GTCCACCTGG AGAGCCAGGC CTACCCGGTA 
     2221 TCCCGGGTCC CATGGGCCCT CCAGGTGCTA TTGGTTTCCC TGGACCCAAA GGAGAAGGTG 
     2281 GAGTTGTAGG ACCACAGGGT CCACCAGGCC CCAAGGGAGA GCCTGGCCTC CAAGGCTTCC 
     2341 CTGGGAAGCC AGGTTTTCTT GGTGAAGTAG GTCCCCCTGG CATGAGGGGT TTGCCTGGTC 
     2401 CTATAGGACC CAAGGGAGAA GGTGGTCACA AAGGGTTGCC AGGGCTTCCC GGTGTTCCAG 
     2461 GGCTGCTTGG ACCCAAAGGA GAACCTGGCA TACCTGGGGA TCAGGGTCTA CAGGGTCCCC 
     2521 CAGGGATTCC AGGGATCGTA GGACCTAGTG GCCCTATTGG ACCCCCTGGG ATTCCGGGCC 
     2581 CCAAAGGAGA ACCAGGCCTC CCAGGGCCCC CTGGGTTCCC TGGTGTAGGG AAGCCAGGAG 
     2641 TAGCAGGACT TCATGGGCCC CCGGGGAAAC CTGGTGCCCT TGGGCCTCAA GGCCAGCCTG 
     2701 GCCTTCCTGG ACCCCCAGGT CCTCCAGGAC CCCCAGGCCC TCCAGCTGTG ATGCCTACAC 
     2761 CATCACCCCA GGGAGAGTAT CTGCCAGATA TGGGACTAGG AATTGATGGG GTGAAACCTC 
     2821 CGCATGCCTA TGCGGGCAAA AAGGGCAAAC ACGGAGGGCC AGCCTATGAG ATGCCTGCGT 
     2881 TTACTGCCGA GCTGACTGTA CCTTTCCCAC CGGTGGGGGC CCCAGTGAAG TTTGACAAGC 
     2941 TGCTCTACAA CGGCAGACAG AACTACAATC CGCAGACAGG CATCTTCACC TGTGAAGTCC 
     3001 CGGGTGTCTA CTACTTTGCT TATCATGTTC ACTGCAAGGG AGGCAACGTA TGGGTTGCTC 
     3061 TCTTCAAGAA CAACGAGCCC ATGATGTACA CATACGACGA GTACAAGAAG GGCTTTCTGG 
     3121 ACCAAGCATC TGGAAGCGCA GTACTGCTTC TCCGTCCCGG AGACCAGGTG TTTCTCCAAA 
     3181 TGCCTTCAGA ACAGGCTGCT GGACTCTATG CCGGGCAATA TGTCCACTCC TCCTTTTCAG 
     3241 GATATTTATT GTATCCCATG TACGCGTACG CGGCCGCTCG AGATGGAGAG CGACGAGAGC 
     3301 GGCCTGCCCG CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG CGTGGAGTTC 
     3361 GAGCTGGTGG GCGGCGGAGA GGGCACCCCC GAGCAGGGCC GCATGACCAA CAAGATGAAG 
     3421 AGCACCAAAG GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT GGGCTACGGC 
     3481 TTCTACCACT TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA CGCCATCAAC 
     3541 AACGGCGGCT ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT GCTGCACGTG 
     3601 AGCTTCAGCT ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT GATGGGCACC 
     3661 GGCTTCCCCG AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA CGCCACCGTG 
     3721 GAGCACCTGC ACCCCATGGG CGATAACGAT CTGGATGGCA GCTTCACCCG CACCTTCAGC 
     3781 CTGCGCGACG GCGGCTACTA CAGCTCCGTG GTGGACAGCC ACATGCACTT CAAGAGCGCC 
     3841 ATCCACCCCA GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG CGTGGAGGAG 
     3901 GATCACAGCA ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA GACCCCGGAT 
     3961 GCAGATGCCG GTGAAGAAAG AGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC 
     4021 AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC 
     4081 CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA 
     4141 GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG 
     4201 GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA 
     4261 ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC 
     4321 CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA 
     4381 GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA 
     4441 CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT 
     4501 CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC 
     4561 TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT 
     4621 TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCTTGGC TGTGGAATGT 
     4681 GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT 
     4741 GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG 
     4801 TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT 
     4861 CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT 
     4921 TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG 
     4981 CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG 
     5041 ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC 
     5101 AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT 
     5161 CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT 
     5221 CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG 
     5281 GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG 
     5341 GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC 
     5401 TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC 
     5461 TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA 
     5521 AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA 
     5581 ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG 
     5641 CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG 
     5701 TGGCCGGCTG GGTGTGGCCG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC 
     5761 TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC 
     5821 CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG 
     5881 GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC 
     5941 GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC 
     6001 CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT 
     6061 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA 
     6121 CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG 
     6181 TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT 
     6241 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT 
     6301 GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG 
     6361 GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG 
     6421 CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG 
     6481 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT 
     6541 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC 
     6601 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC 
     6661 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA 
     6721 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT 
     6781 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG 
     6841 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC 
     6901 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG 
     6961 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC 
     7021 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG 
     7081 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC 
     7141 GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA 
     7201 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA 
     7261 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA 
     7321 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC 
     7381 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA 
     7441 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA 
     7501 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC 
     7561 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT 
     7621 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC 
     7681 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT 
     7741 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC 
     7801 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG 
     7861 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT 
     7921 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG 
     7981 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA 
     8041 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG 
     8101 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG 
     8161 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT 
     8221 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8281 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8341 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8401 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8461 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8521 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8581 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8641 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8701 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8761 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//