LOCUS VC102557 8700 bp DNA circular SYN DEFINITION Myc-DDK-tagged ORF clone for SARS-CoV-2 surface glycoprotein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724390 ACCESSION NC_045512 VERSION NC_045512.2 SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 4857..4948 /label="Myc-DDK" misc_feature 4978..5563 /label="PolyA signal" misc_feature 5035..5054 /label="Restriction Enzyme" misc_feature 5839..6511 /label="ColE1" misc_feature 6941..7735 /label="Kan/Neo" misc_feature 7770..8072 /label="SV40 ori" misc_feature 8158..8635 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 4839..4845 /label="Restriction Enzyme" misc_feature 1020..4839 /label="S" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GTTTGTGTTT CTCGTCCTGC TGCCTCTGGT CTCCTCACAG TGCGTGAATC 1081 TGACAACGAG AACTCAGCTC CCACCCGCAT ATACCAATTC TTTCACCCGG GGTGTGTACT 1141 ACCCTGACAA AGTGTTCAGA AGCTCAGTAC TTCATTCCAC TCAGGACCTG TTTCTTCCAT 1201 TTTTCAGCAA TGTTACCTGG TTTCACGCCA TCCACGTATC CGGGACCAAC GGCACGAAAC 1261 GCTTTGATAA CCCGGTCCTG CCATTCAACG ACGGGGTCTA TTTTGCTTCA ACTGAAAAAA 1321 GCAACATTAT CAGAGGCTGG ATCTTCGGCA CAACTCTGGA CTCAAAGACT CAGTCATTGC 1381 TCATTGTCAA TAACGCAACC AACGTTGTGA TCAAGGTCTG TGAATTCCAG TTCTGCAACG 1441 ATCCGTTTCT GGGCGTATAC TACCACAAGA ACAACAAATC TTGGATGGAG AGTGAATTTC 1501 GAGTTTATTC CAGCGCGAAC AACTGCACCT TCGAATACGT TAGTCAGCCC TTCCTCATGG 1561 ACCTTGAGGG GAAACAAGGC AATTTTAAAA ATCTGCGCGA ATTCGTATTC AAAAACATCG 1621 ATGGCTACTT TAAAATTTAC TCCAAACACA CTCCGATCAA CCTGGTTAGG GACCTGCCCC 1681 AGGGCTTCTC CGCTCTGGAG CCTCTTGTGG ACCTGCCCAT TGGAATTAAT ATCACCCGCT 1741 TTCAGACGCT GCTCGCCCTG CATCGGTCTT ATCTGACTCC CGGCGATTCC TCATCAGGTT 1801 GGACCGCTGG TGCTGCGGCC TATTACGTCG GATATTTGCA GCCTCGGACA TTCCTTCTGA 1861 AATACAATGA GAATGGAACA ATTACAGATG CTGTGGACTG TGCACTCGAC CCGTTGTCCG 1921 AAACCAAATG CACCTTGAAA AGCTTTACTG TGGAAAAGGG CATCTATCAG ACATCTAACT 1981 TCCGGGTTCA ACCTACCGAA TCTATTGTCC GCTTCCCCAA TATTACCAAC CTTTGTCCCT 2041 TCGGTGAGGT ATTCAACGCA ACTCGGTTTG CCTCAGTCTA CGCATGGAAC CGCAAGAGGA 2101 TTTCCAATTG CGTTGCCGAT TATTCAGTCC TTTACAACTC CGCAAGCTTT TCCACGTTTA 2161 AATGCTACGG AGTCTCCCCA ACAAAACTCA ACGACCTGTG CTTTACCAAT GTCTACGCAG 2221 ACTCCTTTGT TATCCGCGGC GACGAGGTTA GGCAAATTGC CCCAGGGCAG ACTGGCAAGA 2281 TCGCCGATTA TAACTATAAG CTCCCCGATG ACTTTACAGG CTGCGTGATC GCATGGAACT 2341 CTAACAACCT CGATAGTAAG GTGGGGGGCA ATTACAATTA TCTTTACCGG CTGTTTCGAA 2401 AGTCAAACCT TAAACCCTTC GAGCGCGATA TTTCAACCGA AATTTATCAG GCCGGGAGCA 2461 CACCATGCAA CGGAGTGGAG GGTTTCAACT GCTACTTCCC ACTGCAGTCT TACGGCTTCC 2521 AGCCCACCAA TGGTGTGGGT TATCAGCCTT ACAGAGTTGT TGTGCTCAGT TTCGAGCTCC 2581 TCCACGCCCC AGCTACCGTT TGCGGACCTA AGAAAAGCAC AAACCTGGTC AAAAATAAAT 2641 GCGTAAACTT CAACTTTAAC GGTCTGACTG GTACCGGCGT TCTCACCGAG TCCAATAAGA 2701 AATTCTTGCC ATTTCAACAG TTTGGTAGAG ACATCGCTGA CACCACCGAT GCTGTCCGGG 2761 ATCCCCAGAC CCTTGAGATT CTCGACATAA CGCCATGCTC ATTCGGGGGT GTCAGCGTTA 2821 TCACTCCGGG CACAAACACT AGCAATCAGG TGGCCGTGCT GTACCAGGAC GTGAACTGCA 2881 CTGAAGTACC CGTGGCCATT CACGCCGATC AGCTGACGCC AACCTGGAGA GTCTACTCAA 2941 CAGGCTCTAA CGTTTTTCAG ACAAGAGCCG GTTGTTTGAT CGGAGCCGAA CACGTAAATA 3001 ATTCATATGA ATGTGACATC CCAATCGGGG CCGGGATCTG CGCTAGTTAC CAGACCCAGA 3061 CTAACTCACC ACGGAGAGCC AGAAGTGTAG CCTCCCAGTC AATAATCGCC TATACAATGT 3121 CTTTGGGCGC GGAAAATAGC GTGGCCTACT CCAATAATTC CATAGCCATT CCCACTAACT 3181 TCACAATTTC CGTCACGACC GAGATCCTGC CGGTGTCAAT GACAAAAACC TCTGTGGACT 3241 GCACAATGTA TATATGTGGC GACAGTACCG AGTGTTCTAA TCTCCTTTTG CAGTATGGGT 3301 CCTTCTGCAC ACAGCTGAAT CGCGCTCTTA CTGGTATCGC TGTGGAGCAA GATAAAAACA 3361 CGCAGGAAGT GTTCGCTCAA GTGAAGCAGA TTTATAAAAC CCCTCCAATC AAAGATTTCG 3421 GCGGATTTAA CTTCTCACAG ATTCTCCCTG ATCCAAGTAA ACCCTCCAAA AGAAGTTTCA 3481 TAGAGGACCT CCTGTTCAAC AAGGTTACGC TGGCTGATGC TGGTTTCATC AAGCAGTATG 3541 GGGACTGCCT CGGCGACATA GCTGCTCGAG ATTTGATCTG CGCCCAGAAA TTCAATGGGC 3601 TGACAGTGCT GCCACCTCTC TTGACAGACG AAATGATTGC ACAATATACT AGTGCCCTTT 3661 TGGCTGGCAC CATCACATCC GGTTGGACTT TTGGTGCGGG AGCCGCACTC CAAATACCCT 3721 TCGCAATGCA GATGGCATAC AGATTCAATG GCATTGGCGT CACCCAGAAC GTTCTCTACG 3781 AAAACCAGAA ACTGATAGCA AATCAGTTTA ACAGCGCTAT CGGCAAGATC CAGGACTCCC 3841 TCTCATCAAC TGCCTCAGCC CTGGGCAAGC TCCAGGATGT GGTAAACCAA AACGCCCAGG 3901 CCCTCAACAC TCTGGTCAAG CAGCTCAGTT CTAACTTCGG AGCCATCTCT AGTGTTCTCA 3961 ATGACATCCT CTCTCGGCTG GACAAGGTGG AAGCAGAGGT GCAGATCGAC CGCCTGATTA 4021 CTGGACGCCT GCAGAGCCTC CAGACCTACG TAACCCAGCA ACTCATCCGG GCTGCCGAGA 4081 TTAGGGCTTC CGCGAACCTC GCCGCCACAA AGATGAGCGA GTGCGTACTT GGACAGAGCA 4141 AGAGAGTGGA TTTTTGTGGC AAGGGGTATC ATCTCATGTC CTTCCCTCAG AGCGCTCCTC 4201 ACGGTGTCGT ATTTCTCCAT GTGACTTATG TCCCCGCCCA AGAGAAAAAC TTCACAACCG 4261 CTCCTGCTAT CTGCCACGAC GGCAAAGCCC ATTTTCCTCG GGAGGGAGTG TTCGTCTCTA 4321 ATGGGACTCA CTGGTTTGTT ACGCAGAGGA ACTTCTACGA GCCTCAGATC ATCACAACAG 4381 ATAACACCTT TGTGTCTGGT AACTGTGACG TGGTCATCGG CATAGTAAAT AACACCGTCT 4441 ACGACCCACT GCAGCCTGAA CTGGATAGCT TTAAGGAAGA GCTCGATAAG TACTTCAAAA 4501 ACCACACAAG CCCGGATGTA GATCTTGGAG ACATTAGTGG GATTAACGCC AGCGTCGTTA 4561 ACATACAAAA AGAGATTGAC CGATTGAACG AAGTTGCCAA AAACCTGAAT GAATCCCTGA 4621 TCGATCTGCA GGAGCTCGGC AAGTACGAGC AGTATATTAA ATGGCCCTGG TACATTTGGC 4681 TGGGCTTCAT CGCGGGGCTC ATCGCGATTG TGATGGTGAC CATTATGCTG TGTTGCATGA 4741 CCAGTTGCTG TAGCTGTCTC AAAGGATGCT GCTCATGTGG CAGCTGTTGC AAGTTCGACG 4801 AGGATGATTC CGAACCCGTA CTGAAAGGCG TGAAACTCCA TTATACTACG CGTACGCGGC 4861 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 4921 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 4981 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 5041 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 5101 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 5161 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 5221 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 5281 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 5341 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 5401 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 5461 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 5521 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 5581 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 5641 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 5701 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 5761 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 5821 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 5881 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 5941 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 6001 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 6061 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 6121 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 6181 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 6241 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 6301 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 6361 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 6421 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 6481 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 6541 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 6601 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 6661 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 6721 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 6781 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 6841 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 6901 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 6961 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 7021 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 7081 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 7141 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 7201 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 7261 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 7321 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 7381 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 7441 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 7501 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 7561 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 7621 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 7681 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 7741 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 7801 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 7861 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 7921 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 7981 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 8041 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 8101 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 8161 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 8221 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 8281 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 8341 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 8401 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 8461 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 8521 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 8581 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 8641 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT //