LOCUS SC318220 7101 bp DNA circular SYN DEFINITION GARS (untagged)-Human glycyl-tRNA synthetase (GARS) ACCESSION NM_002047 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 3258..3349 /label="Myc-DDK" misc_feature 3379..3964 /label="PolyA signal" misc_feature 3436..3455 /label="Restriction Enzyme" misc_feature 4240..4912 /label="ColE1" misc_feature 5342..6136 /label="Kan/Neo" misc_feature 6171..6473 /label="SV40 ori" misc_feature 6559..7036 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 3240..3246 /label="Restriction Enzyme" misc_feature 1020..3240 /label="GARS1" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GCCCTCTCCG CGTCCAGTGC TGCTTAGAGG TGCTCGCGCC GCTCTGCTGC 1081 TGCTGCTGCC GCCCCGGCTC TTAGCCCGAC CCTCGCTCCT GCTCCGCCGG TCCCTCAGCG 1141 CGGCCTCCTG CCCCCCGATC TCCTTGCCCG CCGCCGCCTC CCGGAGCAGC ATGGACGGCG 1201 CGGGGGCTGA GGAGGTGCTG GCACCTCTGA GGCTAGCAGT GCGCCAGCAG GGAGATCTTG 1261 TGCGAAAACT CAAAGAAGAT AAAGCACCCC AAGTAGACGT AGACAAAGCA GTGGCTGAGC 1321 TCAAAGCCCG CAAGAGGGTT CTGGAAGCAA AGGAGCTGGC GTTACAGCCC AAAGATGATA 1381 TTGTAGACCG AGCAAAAATG GAAGATACCC TGAAGAGGAG GTTTTTCTAT GATCAAGCTT 1441 TTGCTATTTA TGGAGGTGTT AGTGGTCTGT ATGACTTTGG GCCAGTTGGC TGTGCTTTGA 1501 AGAACAATAT TATTCAGACC TGGAGGCAGC ACTTTATCCA AGAGGAACAG ATCCTGGAGA 1561 TCGATTGCAC CATGCTCACC CCTGAGCCAG TTTTAAAGAC CTCTGGCCAT GTAGACAAAT 1621 TTGCTGACTT CATGGTGAAA GACGTAAAAA ATGGAGAATG TTTTCGTGCT GACCATCTAT 1681 TAAAAGCTCA TTTACAGAAA TTGATGTCTG ATAAGAAGTG TTCTGTCGAA AAGAAATCAG 1741 AAATGGAAAG TGTTTTGGCC CAGCTTGATA ACTATGGACA GCAAGAACTT GCGGATCTTT 1801 TTGTGAACTA TAATGTAAAA TCTCCCATTA CTGGAAATGA TCTATCCCCT CCAGTGTCTT 1861 TTAACTTAAT GTTCAAGACT TTCATTGGGC CTGGAGGAAA CATGCCTGGG TACTTGAGAC 1921 CAGAAACTGC ACAGGGGATT TTCTTGAATT TCAAACGACT TTTGGAGTTC AACCAAGGAA 1981 AGTTGCCTTT TGCTGCTGCC CAGATTGGAA ATTCTTTTAG AAATGAGATC TCCCCTCGAT 2041 CTGGACTGAT CAGAGTCAGA GAATTCACAA TGGCAGAAAT TGAGCACTTT GTAGATCCCA 2101 GTGAGAAAGA CCACCCCAAG TTCCAGAATG TGGCAGACCT TCACCTTTAT TTGTATTCAG 2161 CAAAAGCCCA GGTCAGCGGA CAGTCCGCTC GGAAAATGCG CCTGGGAGAT GCTGTTGAAC 2221 AGGGTGTGAT TAATAACACA GTATTAGGCT ATTTCATTGG CCGCATCTAC CTCTACCTCA 2281 CGAAGGTTGG AATATCTCCA GATAAACTCC GCTTCCGGCA GCACATGGAG AATGAGATGG 2341 CCCATTATGC CTGTGACTGT TGGGATGCAG AATCCAAAAC ATCCTACGGT TGGATTGAGA 2401 TTGTTGGATG TGCTGATCGT TCCTGTTATG ACCTCTCCTG TCATGCACGA GCCACCAAAG 2461 TCCCACTTGT AGCTGAGAAA CCTCTGAAAG AACCCAAAAC AGTCAATGTT GTTCAGTTTG 2521 AACCCAGTAA GGGAGCAATT GGTAAGGCAT ATAAGAAGGA TGCAAAACTG GTGATGGAGT 2581 ATCTTGCCAT TTGTGATGAG TGCTACATTA CAGAAATGGA GATGCTGCTG AATGAGAAAG 2641 GGGAATTCAC AATTGAAACT GAAGGGAAAA CATTTCAGTT AACAAAAGAC ATGATCAATG 2701 TGAAGAGATT CCAGAAAACA CTATATGTGG AAGAAGTTGT TCCGAATGTA ATTGAACCTT 2761 CCTTCGGCCT GGGTAGGATC ATGTATACGG TATTTGAACA TACATTCCAT GTACGAGAAG 2821 GAGATGAACA GAGAACATTC TTCAGTTTCC CTGCTGTAGT TGCTCCATTC AAATGTTCCG 2881 TCCTCCCACT GAGCCAAAAC CAGGAGTTCA TGCCATTTGT CAAGGAATTA TCGGAAGCCC 2941 TGACCAGGCA TGGAGTATCT CACAAAGTAG ACGATTCCTC TGGGTCAATC GGAAGGCGCT 3001 ATGCCAGGAC TGATGAGATT GGCGTGGCTT TTGGTGTCAC CATTGACTTT GACACAGTGA 3061 ACAAGACCCC CCACACTGCA ACTCTGAGGG ACCGTGACTC AATGCGGCAG ATAAGAGCAG 3121 AGATCTCTGA GCTGCCCAGC ATAGTCCAAG ACCTAGCCAA TGGCAACATC ACATGGGCTG 3181 ATGTGGAGGC CAGGTATCCT CTGTTTGAAG GGCAAGAGAC TGGTAAAAAA GAGACAATCG 3241 AGGAATGAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 3301 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 3361 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 3421 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 3481 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 3541 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 3601 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 3661 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 3721 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 3781 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 3841 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 3901 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 3961 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 4021 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 4081 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 4141 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 4201 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 4261 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 4321 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 4381 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 4441 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 4501 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 4561 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 4621 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 4681 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 4741 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 4801 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 4861 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 4921 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 4981 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 5041 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 5101 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 5161 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 5221 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 5281 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 5341 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 5401 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 5461 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 5521 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 5581 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 5641 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 5701 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 5761 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 5821 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 5881 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 5941 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 6001 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 6061 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 6121 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 6181 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 6241 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 6301 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 6361 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 6421 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 6481 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 6541 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 6601 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 6661 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 6721 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 6781 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 6841 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 6901 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 6961 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 7021 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 7081 CAAAAATTTA ACGCGAATTT T //