LOCUS SC309763 8892 bp DNA circular SYN DEFINITION MAPK8IP3 (untagged)-Human mitogen-activated protein kinase 8 interacting protein 3 (MAPK8IP3), transcript variant 1 ACCESSION NM_015133 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 5049..5140 /label="Myc-DDK" misc_feature 5170..5755 /label="PolyA signal" misc_feature 5227..5246 /label="Restriction Enzyme" misc_feature 6031..6703 /label="ColE1" misc_feature 7133..7927 /label="Kan/Neo" misc_feature 7962..8264 /label="SV40 ori" misc_feature 8350..8827 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 5031..5037 /label="Restriction Enzyme" misc_feature 1020..5031 /label="MAPK8IP3" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GATGGAGATC CAGATGGACG AGGGCGGCGG CGTGGTGGTG TACCAGGACG 1081 ACTACTGCTC CGGCTCGGTG ATGTCGGAGC GGGTGTCGGG CCTGGCGGGC TCCATCTACC 1141 GCGAGTTCGA GCGCCTCATC CACTGCTACG ACGAGGAGGT GGTCAAGGAG CTCATGCCGC 1201 TGGTGGTGAA CGTGCTGGAG AACCTAGACT CGGTGCTCAG CGAGAACCAG GAGCACGAGG 1261 TGGAGCTGGA GCTGCTGCGC GAGGACAACG AGCAGCTGCT CACCCAGTAC GAGCGTGAGA 1321 AGGCGCTGCG CAGGCAGGCG GAGGAGAAAT TCATTGAGTT TGAAGATGCT CTGGAACAAG 1381 AGAAGAAAGA GCTGCAAATC CAGGTGGAGC ACTACGAGTT CCAGACGCGC CAGCTGGAGC 1441 TGAAGGCCAA GAACTATGCC GATCAGATTT CCCGGTTGGA GGAGCGGGAG TCGGAGATGA 1501 AGAAGGAGTA CAATGCCCTG CACCAGCGGC ACACAGAGAT GATACAGACC TACGTGGAGC 1561 ACATTGAGAG GTCCAAGATG CAGCAGGTCG GAGGAAACAG CCAGACCGAG AGCAGCCTGC 1621 CGGGGCGGAG GAAGGAGCGC CCCACCTCCC TGAACGTGTT CCCCCTGGCT GACGGCACGG 1681 TACGTGCACA GATCGGGGGC AAGCTCGTGC CTGCGGGGGA CCACTGGCAC CTGAGTGACC 1741 TCGGCCAGCT GCAGTCCAGC TCCAGCTACC AGTGTCCACA GGATGAAATG TCCGAGTCAG 1801 GCCAGTCCTC GGCGGCCGCC ACACCCAGCA CCACAGGCAC CAAGTCCAAC ACGCCCACAT 1861 CCTCCGTGCC CTCGGCCGCC GTCACACCCC TCAACGAGAG CCTGCAGCCC CTGGGGGACT 1921 ATGGCGTGGG CTCCAAGAAC AGCAAGCGTG CCCGGGAGAA GCGCGACAGC CGCAACATGG 1981 AAGTACAGGT CACCCAGGAG ATGCGCAACG TCAGTATAGG CATGGACAGC AGTGACGAGT 2041 GGTCTGATGT TCAAGACATT ATTGACTCCA CGCCAGAGCT GGACATGTGT CCAGAGACCC 2101 GCCTGGACCG CACAGGAAGC AGCCCAACCC AGGGCATCGT GAACAAAGCT TTCGGCATCA 2161 ACACCGACTC CCTGTACCAT GAGCTGTCGA CGGCAGGGTC TGAGGTCATC GGGGATGTGG 2221 ACGAAGGGGC CGACCTCCTA GGGGAGTTCT CAGTGCGCGA TGATTTCTTT GGAATGGGCA 2281 AAGAAGTGGG GAATCTGCTA CTGGAAAACT CACAGCTTCT GGAAACCAAA AACGCCTTGA 2341 ATGTGGTGAA GAATGACCTG ATTGCCAAGG TCGACCAGCT GTCCGGGGAG CAGGAGGTGC 2401 TGAGGGGCGA GTTGGAGGCT GCTAAGCAGG CCAAAGTCAA GCTGGAAAAC CGTATCAAGG 2461 AGCTGGAAGA GGAACTGAAA AGAGTGAAGT CCGAGGCCAT CATCGCCCGC CGTGAACCCA 2521 AAGAAGAGGC GGAGGATGTA AGCAGCTATC TCTGTACAGA ATCGGACAAA ATCCCCATGG 2581 CCCAGCGCCG CCGCTTCACG CGGGTGGAGA TGGCCCGTGT GCTCATGGAG CGGAACCAGT 2641 ACAAGGAGCG GCTGATGGAG CTGCAGGAGG CTGTGCGGTG GACTGAGATG ATCAGAGCGT 2701 CCCGAGAGCA CCCATCCGTC CAGGAGAAGA AGAAGTCGAC CATCTGGCAG TTCTTCAGCC 2761 GCCTCTTCAG CTCTTCCTCC AGCCCCCCTC CGGCCAAGCG CCCCTATCCC TCGGTGAACA 2821 TCCACTACAA GTCACCCACC ACTGCCGGCT TCAGCCAGCG CCGCAACCAT GCCATGTGCC 2881 CGATCTCGGC AGGCAGCCGG CCCCTGGAAT TCTTCCCTGA CGACGACTGC ACGTCCTCCG 2941 CCCGTCGAGA GCAGAAGCGC GAGCAGTACC GCCAGGTGCG TGAGCACGTG CGTAACGACG 3001 ACGGCCGTCT GCAGGCCTGC GGCTGGAGCC TGCCCGCCAA GTACAAGCAG CTGAGTCCCA 3061 ACGGGGGCCA GGAGGACACG CGGATGAAGA ACGTGCCGGT GCCGGTGTAC TGCCGCCCTC 3121 TGGTGGAGAA GGACCCCACC ATGAAGCTGT GGTGTGCCGC GGGCGTCAAC CTGAGCGGGT 3181 GGAGGCCCAA TGAGGACGAC GCTGGGAATG GAGTCAAGCC AGCGCCAGGC CGCGATCCCC 3241 TGACCTGCGA CCGCGAAGGA GACGGCGAGC CCAAGAGCGC CCACACGTCT CCCGAGAAGA 3301 AGAAGGCCAA GGAGCTCCCT GAAATGGACG CCACCTCCAG CCGGGTGTGG ATCCTGACCA 3361 GCACCCTGAC CACCAGCAAG GTGGTGATCA TCGACGCCAA CCAGCCGGGC ACGGTGGTGG 3421 ACCAGTTCAC CGTCTGCAAC GCGCACGTGC TGTGCATCTC CAGCATCCCC GCGGCCAGCG 3481 ACAGCGACTA CCCTCCCGGG GAGATGTTCC TGGACAGCGA CGTGAACCCA GAGGACCCGG 3541 GCGCAGATGG CGTGCTGGCC GGTATCACCC TGGTGGGCTG TGCCACCCGC TGCAACGTGC 3601 CGCGGAGCAA CTGCTCCTCC CGAGGGGACA CCCCAGTGCT AGACAAGGGG CAGGGGGAGG 3661 TGGCCACCAT CGCCAACGGG AAGGTCAACC CGTCCCAGTC CACAGAGGAG GCCACAGAGG 3721 CCACGGAGGT GCCAGACCCT GGGCCCAGCG AGCCAGAGAC AGCCACATTG CGGCCCGGGC 3781 CTCTCACAGA GCACGTCTTC ACTGACCCAG CCCCGACCCC GTCCTCTGGC CCCCAGCCTG 3841 GCAGCGAGAA CGGGCCAGAG CCTGACAGCA GCAGCACACG GCCAGAGCCA GAGCCCAGCG 3901 GGGACCCCAC GGGAGCAGGC AGCAGTGCTG CACCCACCAT GTGGCTGGGA GCCCAGAACG 3961 GCTGGCTCTA TGTGCACTCG GCTGTGGCCA ACTGGAAGAA GTGCCTGCAC TCCATCAAGC 4021 TGAAGGATTC TGTGCTGAGC CTGGTGCATG TCAAAGGCCG TGTGCTGGTG GCTCTGGCGG 4081 ACGGGACCCT GGCCATCTTC CACCGTGGTG AAGATGGCCA GTGGGATCTG AGCAACTATC 4141 ACCTAATGGA CCTGGGCCAC CCGCACCACT CCATCCGCTG CATGGCTGTT GTGTACGACC 4201 GCGTGTGGTG TGGCTACAAG AACAAGGTGC ACGTCATCCA GCCCAAGACC ATGCAGATAG 4261 AGAAGTCATT TGACGCCCAC CCGCGGCGGG AGAGCCAGGT GCGGCAGCTG GCGTGGATCG 4321 GCGATGGCGT ATGGGTGTCC ATCCGCCTGG ACTCCACCCT GAGGCTCTAC CATGCACACA 4381 CGCACCAGCA TCTACAGGAC GTGGACATTG AGCCCTACGT CAGCAAGATG CTAGGCACTG 4441 GCAAGCTGGG TTTCTCCTTC GTACGCATCA CGGCCCTGCT TGTCGCGGGC AGCCGGCTCT 4501 GGGTGGGCAC CGGCAACGGA GTGGTCATCT CCATCCCCCT GACAGAGACT GTGGTCCTGC 4561 ACCGAGGCCA GCTCCTGGGG CTCCGAGCCA ATAAGACATC CCCCACCTCT GGGGAGGGCG 4621 CCCGTCCCGG GGGCATCATC CACGTGTATG GCGATGACAG CAGTGACAGG GCGGCCAGCA 4681 GCTTCATCCC CTACTGCTCC ATGGCCCAGG CCCAGCTATG CTTCCATGGG CACCGCGATG 4741 CCGTGAAGTT CTTTGTCTCG GTGCCAGGGA ACGTGCTGGC CACCCTGAAT GGCAGTGTGC 4801 TGGACAGCCC AGCCGAGGGC CCTGGGCCAG CTGCCCCTGC CTCGGAGGTC GAGGGCCAGA 4861 AGCTGCGGAA CGTGCTGGTG CTGAGCGGCG GGGAGGGCTA CATCGACTTC CGCATTGGAG 4921 ACGGAGAGGA CGACGAGACG GAGGAGGGCG CAGGGGACAT GAGCCAGGTG AAGCCCGTGC 4981 TGTCCAAGGC AGAGCGCAGT CACATCATCG TGTGGCAGGT GTCCTACACC CCCGAGTGAA 5041 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 5101 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 5161 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 5221 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 5281 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 5341 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 5401 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 5461 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 5521 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 5581 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 5641 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 5701 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 5761 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 5821 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 5881 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 5941 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 6001 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 6061 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 6121 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 6181 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 6241 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 6301 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 6361 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 6421 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 6481 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 6541 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 6601 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 6661 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 6721 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 6781 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 6841 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 6901 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 6961 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 7021 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 7081 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 7141 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 7201 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 7261 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 7321 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 7381 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 7441 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 7501 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 7561 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 7621 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 7681 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 7741 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 7801 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 7861 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 7921 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 7981 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 8041 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 8101 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 8161 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 8221 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 8281 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 8341 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 8401 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 8461 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 8521 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 8581 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 8641 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 8701 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 8761 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 8821 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 8881 AACGCGAATT TT //