LOCUS SC300193 7080 bp DNA circular SYN DEFINITION LSS (untagged)-Human lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (LSS), transcript variant 2 ACCESSION NM_001001438 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 3237..3328 /label="Myc-DDK" misc_feature 3358..3943 /label="PolyA signal" misc_feature 3415..3434 /label="Restriction Enzyme" misc_feature 4219..4891 /label="ColE1" misc_feature 5321..6115 /label="Kan/Neo" misc_feature 6150..6452 /label="SV40 ori" misc_feature 6538..7015 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 3219..3225 /label="Restriction Enzyme" misc_feature 1020..3219 /label="LSS" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GACGGAGGGC ACGTGTCTGC GGCGCCGAGG GGGCCCCTAC AAGACCGAGC 1081 CCGCCACCGA CCTCGGCCGC TGGCGACTCA ACTGCGAGAG GGGCCGGCAG ACGTGGACCT 1141 ACCTGCAGGA CGAGCGCGCC GGCCGCGAGC AGACCGGCCT GGAAGCCTAC GCCCTGGGGC 1201 TGGACACCAA GAATTACTTT AAGGACTTGC CCAAAGCCCA CACCGCCTTT GAGGGGGCTC 1261 TGAACGGGAT GACATTTTAC GTGGGGCTGC AGGCTGAGGA TGGGCACTGG ACGGGTGATT 1321 ATGGTGGCCC ACTTTTCCTC CTGCCAGGCC TCCTGATCAC TTGCCACGTG GCACGCATCC 1381 CTCTGCCAGC CGGATACAGA GAAGAGATTG TGCGGTACCT GCGGTCAGTG CAGCTCCCTG 1441 ACGGTGGCTG GGGCCTGCAC ATTGAGGATA AGTCCACCGT GTTTGGGACT GCGCTCAACT 1501 ATGTGTCTCT CAGAATTCTG GGTGTTGGGC CTGACGATCC TGACCTGGTA CGAGCCCGGA 1561 ACATTCTTCA CAAGAAAGGT GGTGCTGTGG CCATCCCCTC CTGGGGGAAG TTCTGGCTGG 1621 CTGTCCTGAA TGTTTACAGC TGGGAAGGCC TCAATACCCT GTTCCCAGAG ATGTGGCTGT 1681 TTCCTGACTG GGCACCGGCA CACCCCTCCA CACTCTGGTG CCACTGCCGG CAGGTGTACC 1741 TGCCCATGAG CTACTGCTAC GCCGTTCGGC TGAGTGCCGC GGAAGACCCG CTGGTCCAGA 1801 GCCTCCGCCA GGAGCTCTAT GTGGAGGACT TCGCCAGCAT TGACTGGCTG GCGCAGAGGA 1861 ACAACGTGGC CCCCGACGAG CTGTACACGC CGCACAGCTG GCTGCTCCGC GTGGTATATG 1921 CGCTCCTCAA CCTGTATGAG CACCACCACA GTGCCCACCT GCGGCAGCGG GCCGTGCAGA 1981 AGCTGTATGA ACACATTGTG GCCGACGACC GATTCACCAA GAGCATCAGC ATCGGCCCGA 2041 TCTCGAAAAC CATCAACATG CTTGTGCGCT GGTATGTGGA CGGGCCCGCC TCCACTGCCT 2101 TCCAGGAGCA TGTCTCCAGA ATCCCGGACT ATCTCTGGAT GGGCCTTGAC GGCATGAAAA 2161 TGCAGGGCAC CAACGGCTCA CAGATCTGGG ACACCGCATT CGCCATCCAG GCTCTGCTTG 2221 AGGCGGGCGG GCACCACAGG CCCGAGTTTT CGTCCTGCCT GCAGAAGGCT CATGAGTTCC 2281 TGAGGCTCTC ACAGGTCCCA GATAACCCTC CCGACTACCA GAAGTACTAC CGCCAGATGC 2341 GCAAGGGTGG CTTCTCCTTC AGTACGCTGG ACTGCGGCTG GATCGTTTCT GACTGCACGG 2401 CTGAGGCCTT GAAGGCTGTG CTGCTCCTGC AGGAGAAGTG TCCCCATGTC ACCGAGCACA 2461 TCCCCAGAGA ACGGCTCTGC GATGCTGTGG CTGTGCTGCT GAACATGAGA AATCCAGATG 2521 GAGGGTTCGC CACCTATGAG ACCAAGCGTG GGGGGCACTT GCTGGAGCTG CTGAACCCCT 2581 CGGAGGTCTT CGGGGACATC ATGATTGACT ACACCTATGT GGAGTGCACC TCAGCCGTGA 2641 TGCAGGCGCT TAAGTATTTC CACAAGCGTT TCCCGGAGCA CAGGGCAGCG GAGATCCGGG 2701 AGACCCTCAC GCAGGGCTTA GAGTTCTGTC GGCGGCAGCA GAGGGCCGAT GGCTCCTGGG 2761 AAGGCTCCTG GGGAGTTTGC TTCACCTACG GCACCTGGTT TGGCCTGGAG GCCTTCGCCT 2821 GTATGGGGCA GACCTACCGA GATGGGACTG CCTGTGCAGA GGTCTCCCGG GCCTGTGACT 2881 TCCTGCTGTC CCGGCAGATG GCAGACGGAG GCTGGGGGGA GGACTTTGAG TCCTGCGAGG 2941 AGCGGCGTTA TTTGCAGAGT GCCCAGTCCC AGATCCATAA CACATGCTGG GCCATGATGG 3001 GGCTGATGGC CGTTCGGCAT CCTGACATCG AGGCCCAGGA GAGAGGAGTC CGGTGTCTAC 3061 TTGAGAAACA GCTCCCCAAT GGCGACTGGC CGCAGGAAAA CATTGCTGGG GTCTTCAACA 3121 AGTCCTGTGC CATCTCCTAC ACGAGCTACA GGAACATCTT CCCCATCTGG GCCCTCGGCC 3181 GCTTCTCCCA GCTGTACCCT GAGAGAGCCC TTGCTGGCCA CCCCTGAACG CGTACGCGGC 3241 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 3301 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 3361 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 3421 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 3481 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 3541 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 3601 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 3661 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 3721 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 3781 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 3841 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 3901 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 3961 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 4021 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 4081 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 4141 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 4201 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 4261 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 4321 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 4381 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 4441 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 4501 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 4561 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 4621 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 4681 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 4741 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 4801 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 4861 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 4921 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 4981 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 5041 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 5101 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 5161 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 5221 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 5281 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 5341 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 5401 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 5461 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 5521 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 5581 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 5641 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 5701 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 5761 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 5821 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 5881 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 5941 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 6001 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 6061 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 6121 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 6181 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 6241 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 6301 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 6361 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 6421 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 6481 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 6541 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 6601 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 6661 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 6721 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 6781 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 6841 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 6901 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 6961 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 7021 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT //