LOCUS       RR217651                9456 bp    DNA     circular  SYN 
DEFINITION  Slit2 (myc-DDK-tagged) - Rat slit homolog 2 (Drosophila) (Slit2)
ACCESSION   NM_022632
VERSION     NM_022632.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5613..5704 
                     /label="Myc-DDK"  
     misc_feature    5734..6319 
                     /label="PolyA signal"  
     misc_feature    5791..5810 
                     /label="Restriction Enzyme"  
     misc_feature    6595..7267 
                     /label="ColE1"  
     misc_feature    7697..8491 
                     /label="Kan/Neo"  
     misc_feature    8526..8828 
                     /label="SV40 ori"  
     misc_feature    8914..9391 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5595..5601 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5595 
                     /label="Slit2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTGGCATT GGCTGGCAGA CACTGTCCCT ATCTCTGGCG TTAGTGTTGT 
     1081 CGATCTTGAA CCAGGTGGCG CCTCAGGCGT GCCCGGCCCA GTGCTCCTGT TCAGGCAGCA 
     1141 CAGTGGACTG TCATGGGCTG GCACTGCGCA GTGTGCCCAG GAATATCCCC CGCAACACGG 
     1201 AGAGACTGGA TTTGAATGGA AATAACATCA CAAGGATCAC GAAGACAGAT TTTGCGGGTC 
     1261 TCAGACACCT CAGAGTTCTT CAGCTCATGG AGAACAAGAT CAGCACCATC GAGAGGGGAG 
     1321 CATTCCAGGA TCTTAAGGAG CTAGAAAGAC TGCGTTTAAA CAGAAATAAC CTTCAGTTGT 
     1381 TTCCTGAGCT GCTGTTTCTT GGGACTGCGA AGCTCTACCG GCTTGATCTC AGTGAAAATC 
     1441 AGATTCAAGC AATTCCAAGG AAGGCTTTCC GTGGTGCAGT TGACATTAAA AATCTGCAGT 
     1501 TGGATTACAA CCAGATCAGC TGCATTGAAG ATGGGGCATT CCGAGCTCTG CGAGATCTGG 
     1561 AAGTGCTCAC TCTGAACAAT AACAATATTA CTAGACTTTC AGTGGCAAGT TTCAACCATA 
     1621 TGCCTAAACT TAGGACATTT CGACTCCACT CCAACAACCT ATACTGCGAC TGCCACCTGG 
     1681 CCTGGCTCTC GGACTGGCTT CGCCAAAGGC CACGGGTGGG CTTGTACACT CAGTGTATGG 
     1741 GCCCATCCCA CCTGAGGGGC CATAATGTAG CAGAGGTTCA AAAACGAGAG TTTGTCTGCA 
     1801 GTGATGAGGA AGAAGGTCAC CAGTCATTCA TGGCTCCCTC CTGCAGTGTG CTGCACTGCC 
     1861 CGATTGCTTG TACCTGTAGC AACAACATTG TAGACTGCCG AGGGAAAGGT CTCACTGAGA 
     1921 TCCCCACAAA TCTGCCTGAG ACCATCACAG AAATACGTTT GGAACAGAAC TCCATAAGGG 
     1981 TCATCCCTCC AGGAGCATTC TCACCATACA AAAAGCTTCG ACGACTAGAC CTGAGTAATA 
     2041 ACCAGATCTC GGAACTTGCT CCAGATGCCT TCCAAGGACT GCGTTCTCTG AATTCCCTTG 
     2101 TCCTGTATGG AAATAAAATC ACAGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT 
     2161 TACAGCTACT ATTATTGAAT GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG 
     2221 ACCTGCACAA CTTGAACCTT CTCTCCTTAT ACGACAATAA GCTTCAGACT GTTGCCAAGG 
     2281 GCACCTTCTC AGCTCTCAGA GCCATCCAAA CTATGCATTT GGCCCAGAAT CCTTTCATTT 
     2341 GTGACTGCCA TCTCAAGTGG CTAGCGGATT ATCTCCACAC CAACCCAATT GAGACCAGCG 
     2401 GTGCCCGTTG CACCAGTCCC CGCCGCCTGG CTAACAAAAG AATTGGACAG ATCAAAAGCA 
     2461 AGAAATTCCG TTGTTCAGGT ACAGAAGATT ATCGATCAAA ATTAAGTGGA GACTGCTTTG 
     2521 CAGACTTGGC TTGTCCTGAA AAATGTCGCT GTGAAGGGAC CACAGTAGAC TGCTCCAATC 
     2581 AAAAACTCAA CAAAATCCCA GACCATATTC CCCAGTACAC AGCAGAGCTG CGTCTCAATA 
     2641 ATAATGAATT CACAGTGTTA GAAGCCACGG GAATATTTAA GAAACTTCCT CAATTGCGTA 
     2701 AAATCAACCT TAGCAACAAT AAGATCACTG ATATCGAGGA GGGGGCATTC GAAGGTGCGT 
     2761 CTGGTGTGAA TGAGATTCTG CTTACCAGTA ACCGTTTGGA AAATGTTCAG CATAAGATGT 
     2821 TCAAAGGATT GGAGAGCCTC AAAACATTGA TGCTGAGAAG TAATCGAATA AGCTGTGTGG 
     2881 GAAACGACAG TTTCACAGGA CTCGGTTCTG TGCGTCTGCT CTCTTTATAT GACAATCAAA 
     2941 TTACCACAGT TGCACCAGGA GCATTTGGTA CTCTCCATTC ATTATCTACA CTAAACCTCT 
     3001 TGGCCAATCC TTTCAACTGT AACTGTCACC TGGCATGGCT TGGAGAATGG CTCAGAAGGA 
     3061 AAAGAATTGT AACAGGAAAT CCTCGATGCC AAAAACCCTA CTTCTTGAAG GAAATACCAA 
     3121 TCCAGGATGT AGCCATTCAG GACTTCACCT GTGATGACGG AAACGATGAT AATAGCTGCT 
     3181 CTCCACTCTC CCGTTGTCCT TCGGAATGTA CTTGCTTGGA TACAGTAGTA CGATGTAGCA 
     3241 ACAAGGGCTT GAAGGTCTTA CCTAAAGGCA TTCCAAGAGA TGTCACAGAA CTGTATCTGG 
     3301 ATGGGAACCA GTTTACACTG GTCCCGAAGG AACTCTCCAA CTACAAACAT TTAACACTTA 
     3361 TAGACTTAAG TAACAACAGA ATAAGCACCC TTTCCAACCA AAGCTTCAGC AACATGACCC 
     3421 AACTTCTCAC CTTAATTCTC AGTTACAACC GTCTGAGATG TATCCCTCCA CGGACCTTTG 
     3481 ATGGATTGAA ATCTCTTCGT TTACTGTCTC TACATGGAAA TGACATTTCT GTCGTGCCTG 
     3541 AAGGTGCCTT TGGTGACCTT TCAGCCTTGT CACACTTAGC AATTGGAGCC AACCCTCTTT 
     3601 ACTGTGATTG TAACATGCAG TGGTTATCCG ACTGGGTGAA GTCGGAATAT AAGGAACCTG 
     3661 GAATTGCCCG CTGTGCCGGT CCCGGAGAAA TGGCAGATAA ATTGTTACTC ACAACTCCCT 
     3721 CCAAAAAATT TACATGTCAA GGTCCTGTGG ATGTTACTAT TCAAGCCAAG TGTAACCCCT 
     3781 GCTTGTCAAA TCCATGTAAA AATGATGGCA CCTGTAACAA TGACCCGGTG GATTTTTATC 
     3841 GATGCACCTG CCCATATGGT TTCAAGGGCC AGGACTGTGA TGTCCCCATT CATGCCTGTA 
     3901 TCAGTAATCC ATGTAAACAT GGAGGAACTT GCCACTTAAA AGAAGGAGAG AATGATGGAT 
     3961 TCTGGTGTAC TTGTGCTGAT GGGTTTGAAG GAGAAAGCTG TGACATCAAT ATTGATGATT 
     4021 GCGAAGATAA TGATTGTGAA AATAATTCTA CATGCGTTGA TGGAATTAAC AACTACACGT 
     4081 GTCTTTGCCC ACCGGAATAC ACAGGCGAAC TGTGTGAGGA AAAACTGGAC TTCTGTGCAC 
     4141 AAGACCTGAA TCCCTGCCAG CATGACTCCA AGTGCATCCT GACGCCAAAG GGATTCAAGT 
     4201 GTGACTGCAC TCCGGGATAC ATTGGTGAGC ACTGTGACAT CGACTTTGAT GACTGCCAAG 
     4261 ATAACAAGTG CAAAAACGGT GCTCATTGCA CAGATGCAGT GAACGGATAC ACATGTGTCT 
     4321 GTCCTGAAGG CTACAGTGGC TTGTTCTGTG AGTTTTCTCC ACCCATGGTC CTCCCTCGCA 
     4381 CCAGCCCCTG TGATAATTTT GATTGTCAGA ATGGAGCCCA GTGTATCATC AGGGTGAATG 
     4441 AACCAATATG CCAGTGTTTG CCTGGCTACT TGGGAGAGAA GTGTGAGAAA TTGGTCAGTG 
     4501 TGAATTTTGT AAACAAAGAG TCCTATCTTC AGATTCCTTC AGCCAAGGTT CGACCTCAGA 
     4561 CAAACATCAC ACTTCAGATT GCCACAGATG AAGACAGCGG CATCCTCTTG TACAAGGGTG 
     4621 ACAAGGACCA CATTGCTGTG GAACTCTATC GAGGGCGAGT TCGAGCCAGC TATGACACCG 
     4681 GCTCTCACCC GGCTTCTGCC ATTTACAGTG TGGAGACAAT CAATGATGGA AACTTCCACA 
     4741 TTGTAGAGCT ACTGACCCTG GATTCGAGTC TTTCCCTCTC TGTGGATGGA GGAAGCCCTA 
     4801 AAATCATCAC CAATTTGTCA AAACAATCTA CTCTGAATTT CGACTCTCCA CTTTACGTAG 
     4861 GAGGTATGCC TGGGAAAAAT AACGTGGCTT CGCTGCGCCA GGCCCCTGGG CAGAACGGCA 
     4921 CCAGCTTCCA TGGCTGTATC CGGAACCTTT ACATTAACAG TGAACTGCAG GACTTCCGGA 
     4981 AAGTGCCTAT GCAAACCGGA ATTCTGCCTG GCTGTGAACC ATGCCACAAG AAAGTGTGTG 
     5041 CCCATGGCAC ATGCCAGCCC AGCAGCCAAT CAGGCTTCAC CTGTGAATGT GAGGAAGGGT 
     5101 GGATGGGGCC CCTCTGTGAC CAGAGAACCA ATGATCCCTG TCTCGGAAAC AAATGTGTAC 
     5161 ATGGGACCTG CTTGCCCATC AACGCCTTCT CCTACAGCTG CAAGTGCCTG GAGGGCCACG 
     5221 GCGGGGTCCT CTGTGATGAA GAAGAAGATC TGTTTAACCC CTGCCAGGTG ATCAAGTGCA 
     5281 AGCACGGGAA GTGCAGGCTC TCTGGGCTCG GGCAGCCCTA TTGTGAATGC AGCAGTGGAT 
     5341 TCACCGGGGA CAGCTGTGAC AGAGAAATTT CTTGTCGAGG GGAACGGATA AGGGATTATT 
     5401 ACCAAAAGCA GCAGGGTTAC GCTGCCTGTC AAACGACTAA GAAAGTATCT CGCTTGGAGT 
     5461 GCAGAGGCGG GTGTGCTGGG GGGCAGTGCT GTGGACCTCT GAGAAGCAAG AGGCGGAAAT 
     5521 ACTCTTTCGA ATGCACAGAT GGATCTTCAT TTGTGGACGA GGTCGAGAAG GTGGTGAAGT 
     5581 GCGGCTGCAC GAGATGTGCC TCCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5641 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5701 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5761 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5821 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5881 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5941 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     6001 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     6061 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     6121 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     6181 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     6241 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     6301 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     6361 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     6421 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6481 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6541 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6601 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6661 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6721 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6781 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6841 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6901 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6961 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     7021 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     7081 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     7141 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     7201 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     7261 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     7321 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     7381 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     7441 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7501 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7561 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7621 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7681 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7741 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7801 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7861 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7921 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7981 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     8041 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     8101 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     8161 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     8221 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     8281 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     8341 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     8401 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     8461 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8521 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8581 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8641 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8701 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8761 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8821 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8881 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8941 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     9001 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     9061 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     9121 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     9181 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     9241 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     9301 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     9361 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     9421 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//