LOCUS       RR217649                9426 bp    DNA     circular  SYN 
DEFINITION  Robo2 (myc-DDK-tagged) - Rat roundabout, axon guidance receptor, homolog 2 (Drosophila) (Robo2)
ACCESSION   NM_032106
VERSION     NM_032106.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5581..5672 
                     /label="Myc-DDK"  
     misc_feature    5702..6287 
                     /label="PolyA signal"  
     misc_feature    5759..5778 
                     /label="Restriction Enzyme"  
     misc_feature    6563..7235 
                     /label="ColE1"  
     misc_feature    7665..8459 
                     /label="Kan/Neo"  
     misc_feature    8494..8796 
                     /label="SV40 ori"  
     misc_feature    8882..9359 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5556..5563 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5556 
                     /label="Robo2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTCCTCTG ATGTTTACAC TACTATTGCT CTTTGGATTT CTCCGCATTC 
     1081 GGACTGACGG ATNTCGTCTT CGTCAAGAAG ACTTTCCCCC CAAGATNGTA GAACAACCTT 
     1141 CTGAAGTCAT CGTCTCCAAA GGAAAACCCA ACACTCCGAA CTGGAAGCAA AAGGGCCGGC 
     1201 CATTCCCAAC AATTGGAAAG GTACAAAGGA TGGTTAAACC GGGTTGGGAC AAGACCAAGG 
     1261 ATGATTCCAA GGTCACACAA GGATGCCTTC TGCCCAGCGG ATCCTTATTC TTTTTGCGAA 
     1321 TTGTTCACGG ACGCAGAAGT AAACCAGATG AAGGAACTTA CGTTTGTGTT GCAAGGAACT 
     1381 ATCTTGGTGA AGCAGTGAGT CGCAATGCAT CTCTGGAAGT AGCATTATTG CGAGATGACT 
     1441 TCCGGCAAAA CCCCACAGAT GTGGTAGTCG CAGCAGGAGA GCCTGCAATC TTGGAGTGCC 
     1501 AGCCACCACG GGGACACCCA GAACCAACCA TCTACTGGAA AAAGGACAAA GTCCGAATTG 
     1561 ATGAGAAGGA AGAGAGAATA AGTATCCGTG GTGGGAAGCT GATGATCTCC AATACTAGGA 
     1621 AAAGCGATGC TGGCATGTAT ACCTGTGTGG GAACCAACAT GGTGGGAGAA AGGGACAGCG 
     1681 ACCCTGCAGA GCTCACTGTC TTTGAACGAC CCACGTTTCT CAGGAGGCCA ATCAACCAGG 
     1741 TGGTGCTGGA GGACGAGCCT GCAGAATTCC GCTGTCAGGT CCAAGGAGAT CCCCAGCCAA 
     1801 CGGTGAGGTG GAAAAAGGAT GACGCAGACT TACCAAGAGG AAGGTATGAT ATCAAGGACG 
     1861 ACTACACCCT GAGAATCAAA AAGGCCATAA GTGCAGATGA AGGCACCTAT GTGTGCATTG 
     1921 CTGAGAACCG GGTGGGAAAA GTGGAAGCTT CTGCCACCCT CACTGTCCGA GCTCCTCCAC 
     1981 AGTTTGTGGT TAGGCCAAGA GATCAGATCG TTGCTCAGGG CCGGACAGTG ACATTCCCCT 
     2041 GTGAAACTAA AGGAAACCCA CAGCCAGCTG TCTTCTGGCA GAAAGAAGGC AGCCAGAACC 
     2101 TACTTTTCCC GAATCAACCT CAGCAGCCCA ATAGCCGCTG TTCAGTGTCG CCCACGGGGG 
     2161 ACCTCACCAT CACCAACATT CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA 
     2221 CTGTGGCAGG AAGCATTTTA GCTAAAGCAC AGCTGGAAGT TACTGACGTT TTGACAGATA 
     2281 GACCTCCACC CATAATCTTG CAAGGACCAA TAAACCAAAC ACTTGCGGTA GATGGCACAG 
     2341 CATTACTGAA ATGTAAAGCC ACTGGACCTC TTCCTGTAAT TAGCTGGCTA AAGGAGGGCT 
     2401 TTACTTTTCT GGGTAGAGAT CCAAGAGCTA CGATCCAAGA CCAAGGAACA CTGCAGATTA 
     2461 AGAATTTACG GATTTCTGAT ACTGGCACTT ATACATGTGT GGCTACAAGT TCGAGTGGGG 
     2521 AGACTTCCTG GAGTGCAGTG CTGGATGTGA CAGAGTCTGG AGCAACAATC AGTAAAAATT 
     2581 ATGATACAAA TGACCTGCCC GGGCCACCAT CCAAACCTCA GGTCACTGAT GTTACGAAGA 
     2641 ACAGTGTCAC CCTATCCTGG CAGACAGGTA CCCCTGGCGT TCTTCCAGCA AGCGCATATA 
     2701 TCATTGAGGC TTTCAGCCAA TCGGTGAGCA ACAGCTGGCA GACAGTGGCA AACCATGTTA 
     2761 AGACGACTCT CTATACAGTC AGAGGACTAA GGCCCAACAC AATCTATCTG TTCATGGTCA 
     2821 GAGCGATCAA CCCCCAAGGT CTCAGTGATC CAAGCCCCAT GTCGGATCCT GTACGCACAC 
     2881 AAGATATCAG TCCCCCAGCA CAAGGCGTGG ACCACAGACA AGTGCAGAAG GAGTTAGGAG 
     2941 ATGTCACTGT TCGTCTCCAC AATCCAGTTG TCCTGACACC TACAACTGTT CAAGTCACAT 
     3001 GGACGGTGGA CCGACAACCC CAGTTTATTC AGGGCTACAG AGTGATGTAC CGACAGACTT 
     3061 CTGGTCTACA AGCTTCAACT GTGTGGCAGA ATCTAGATGC CAAAGTCCCG ACTGAGAGGA 
     3121 GTGCTGTCCT TGTGAATCTG AAAAAGGGGG TGACTTATGA AATTAAAGTC AGGCCATATT 
     3181 TCAATGAGTT CCAAGGAATG GACAGTGAAT CAAAAACAAT CCGTACTACT GAGGAAGCTC 
     3241 CAAGTGCGCC TCCCCAGTCT GTCACGGTGC TGACCGTTGG AAGCCATAAC AGCACAAGCA 
     3301 TTAGTGTTTC CTGGGATCCT CCACCAGCCG ACCACCAGAA TGGAATCATA CAGGAATACA 
     3361 AGATCTGGTG TCTGGGAAAT GAAACGCGAT TCCATATCAA TAAAACTGTG GATGCAACCA 
     3421 TTCGCTCTGT AGTAATAGGT GGCTTGTTCC CTGGAATTCA GTACCGGGTG GAAGTGGCAG 
     3481 CTAGTACAAG TGCAGGGGTT GGAGTAAAAA GTGAACCACA GCCAATAATC ATTGGGGGAC 
     3541 GTAACGAAGT TGTCATTACT GAAAACAATA ACAGCATAAC TGAGCAAATC ACTGATGTCG 
     3601 TGAAGCAACC AGCATTTATA GCTGGCATCG GTGGTGCCTG CTGGGTAATT TTGATGGGTT 
     3661 TTAGCATCTG GTTATACTGG AGAAGAAAGA AGAGAAAGGG ACTTAGCAAT TATGCTGTAA 
     3721 CATTTCAAAG AGGAGATGGA GGACTAATGA GCAATGGGAG CCGTCCAGGT CTTCTAAATA 
     3781 CTGGCGACCC CAATTATCCA TGGCTTGCTG ACTCATGGCC AGCCACGAGT TTGCCAGTCA 
     3841 ACAACAGCAA TAGTGGCCCA AATGAAATCG GAAATTTTGG GCGTGGAGAT GTGCTGCCTC 
     3901 CAGTCCCAGG CCAAGGGGAT AAAACAGCAA CAATGCTCTC AGATGGAGCT ATTTATAGTA 
     3961 GCATTGACTT CACTACCAAG ACCACTTACA ACAGTTCCAG CCAAATAACG CAGGCCACCC 
     4021 CATACGCCAC TACCCAGATC CTGCATTCCA ACAGCATCCA TGAACTGGCG GTTGATCTTC 
     4081 CTGATCCACA GTGGAAAAGC TCAGTTCAAC AGAAATCAGA CCTCATGGGA TTTGCTTATT 
     4141 CACTACCTGA TCAGAACAAG GGGAACAATG CCTTACTTTA CATCCCTGAC TACCGATTGG 
     4201 CTGAGGGATT GTCTAATAGA ATGCCACACA ACCAGTCACA GGATTTCAGC ACCACCAGCT 
     4261 CTCACAACAG CTCAGAAAGG AGTGGCAGTC TCTCAGGTGG GAAAGGTGGA AAAAAGAAGA 
     4321 AAACTAAAAA TTCTTCAAAG GCGCAAAAAA ACAACGGATC CACTTGGGCT AATGTCCCTC 
     4381 TACCTCCTCC TCCTGTCCAG CCCCTTCCGG GTACAGAGCT CGGACACTAT CCTGCGGAAC 
     4441 AGGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT ACCAGTGCAA ACCTATTTGC 
     4501 ATCAGGGAAT GGAAGACGAG TTGGAAGAGG ATGAAGATCG CGTCCCAACA CCTCCTGTTC 
     4561 GCGGTGTGGC CTCTTCACCC GCTATCTCCT TTGGGCAGCA GTCCACTGCC ACTCTTACAC 
     4621 CATCCCCACG GGAGGAGATG CAACCCATGC TGCAAGCTCA CTTGGATGAG TTAACCAGGG 
     4681 CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATACT CCACCTCCAC 
     4741 AACCCCCAGT TCCACCATTA GGTTACGCGT CCGGAGCCTT GATTTCTGAC TTGGAGACAG 
     4801 ATGTTCCAGA TGAGGATGCT GATGATGAAG AGGAACCATT AGAAATTCCC AGGCCCCTCA 
     4861 GAGCACTAGA CCAGACTCCT GGTTCCAGTA TGGACAATCT AGACAGCTCT GTAACAGGAA 
     4921 AAGCCTTTAC TTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACA GACAGTAACA 
     4981 CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC CAAAAAACAC AAGGGAGGGC 
     5041 GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT GATCTTCCCC 
     5101 CACCACCAGA TCCCCCACCA GGTCAGGGTT TAAGGCAGCA GATAGGCCTG AGCCAACACT 
     5161 CTGGTAACGT GGAAAGCTCA ACAGAGAGGA AAGGAAGCTC TCTAGAGAGA CAACAAGCAG 
     5221 CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCGGC TAAAACTACT GTAGAGTGGC 
     5281 AGCATCAAAC CCAGGACTGG ATAAACTCTA CAGAGCGCCA AGAAGAAACA CGGAAAGCCC 
     5341 CACACAAACC AGGGGTTGGA TCAGAGGAGT CCCTGGTGCC CTACAGCAAG CCCAGCTTCC 
     5401 CATCTCCAGG CGGGCACAGC TCATCCGGAA CAGCCTCCTC TAAAGGGTCC ACTGGTCCTC 
     5461 GGAAGGCTGA GATTCTGAGG GGCAGCCACC AGCGGAATGC CAATGACCTT CTCGACATTG 
     5521 GATATGTGGG CTCAAATAGT CAAGGGCAGT TTACAGGTGA ATTGAGCGGA CCGACGCGTA 
     5581 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5641 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5701 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5761 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5821 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5881 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5941 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     6001 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     6061 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     6121 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     6181 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     6241 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     6301 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     6361 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     6421 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     6481 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6541 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6601 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6661 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6721 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6781 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6841 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6901 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6961 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     7021 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     7081 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     7141 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     7201 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     7261 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     7321 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     7381 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     7441 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     7501 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7561 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7621 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7681 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7741 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7801 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7861 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7921 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7981 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     8041 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     8101 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     8161 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     8221 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     8281 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     8341 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     8401 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     8461 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     8521 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8581 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8641 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8701 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8761 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8821 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8881 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8941 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     9001 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     9061 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     9121 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     9181 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     9241 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     9301 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     9361 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     9421 AATTTT
//