LOCUS       RR217646                9372 bp    DNA     circular  SYN 
DEFINITION  Gemin5 (myc-DDK-tagged) - Rat gem (nuclear organelle) associated protein 5 (Gemin5)
ACCESSION   NM_001172089
VERSION     NM_001172089.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5529..5620 
                     /label="Myc-DDK"  
     misc_feature    5650..6235 
                     /label="PolyA signal"  
     misc_feature    5707..5726 
                     /label="Restriction Enzyme"  
     misc_feature    6511..7183 
                     /label="ColE1"  
     misc_feature    7613..8407 
                     /label="Kan/Neo"  
     misc_feature    8442..8744 
                     /label="SV40 ori"  
     misc_feature    8830..9307 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5511..5517 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5511 
                     /label="Gemin5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAACCAGAG CCGCGGACGC TTCCGCCGTC CCCCAACTGG TACTGCGCGC 
     1081 GCTGCAGCGA CGCGGCGCCC GGAGGCATCT TTGGCTTCGC TGCGCGGACC TCCGTCTTCC 
     1141 TCGTCCGCGT GGGCCCGGGC GCCGGCGCGA GCCCAGGGGC GCCCCCGTTT CGAGTTGTAG 
     1201 GAGAGTTGGT GGGACACACT GAAAGAGTAT CTGGTTTCAC TTTTTCTCAT CACCCTGGAC 
     1261 AATACAACCT CTGTGCCACC AGCTCAGATG ATGGGACTGT GAAGATTTGG GATGTAGAGA 
     1321 CCAAGGCTGT AGTGACAGAA CACGCGCTCC ATCAGCATAC AATATCAGCA CTGCATTGGT 
     1381 CTCCGACAGT TAAGGACTTA ATAGTCTCTG GGGATGAAAA AGGAGTTGTT TTCTGTTACT 
     1441 GGCTTAACAG AAGTGACAGC CAGCACCTCT TCACAGAGCC CAGGGCCATA TTCTGTCTGA 
     1501 CCTGTTCACC TCATCACGAA AATCTAGTAG CCATTGGCTA CAAAGACGGC ATAGTGATTA 
     1561 TCATTGACAT CAGTAAAAAG GGAGAAGTTG TTCACAGGCT TCGAGGGCAC GACGATGAAA 
     1621 TCCACTCCAT AGCCTGGTGT CCTTTGTCTG GTGAAGACTG CTTATCTATA AACCAAGAGG 
     1681 AAAACTCAGA AGAACCTGAC ATTCCCAATG GGAAGCTTAT AGCAGAAACC CCCATCACAA 
     1741 AAGGCTGCTA CTTAGCTACA GGAAGCAAGG ATCAGACCAT TCGAATCTGG AGCTGTTCCC 
     1801 GAGGCCGGGG TGTAATGGTT TTGAAATTGC CCTTTCTGAA GAGAAGAAGT GGGGGTGTGG 
     1861 ACCCAACAGT TAAAGAACGT CTTTGGTTGA CACTTCATTG GCCCAAGAAC CAACCAACAC 
     1921 AGCTGGTATC CAGTTGTTTT GGAGGTGAGC TGCTGCTGTG GGACCTCACC CAGTCATGGA 
     1981 GACGGAAGTA CACGCTCTTC AGCACCTCAG CGGAAGGGCA CAATCACTCC AGAATTGTCT 
     2041 TCAACTTGTG TCCTTTGAAG ACTGAGGATG GCAAACAGCT GCTATTGTCC ACGTCCATGG 
     2101 ACAGAGATGT AAAATGTTGG GACATGGCCA CCCTGGAGTG TTGCTGGACA TTACCTTCTC 
     2161 TTGGAGGTTT TGCCTACAGC CTGGCCTTTT CACCTGTGGA CGTGGGCTCC CTGGCCATAG 
     2221 GTGTTGGGGA CGGCATGATC CGGGTATGGA ATACACTCTC CATAAAGAAC AACTACGATG 
     2281 TGAAAAACTT CTGGCAAGGA GTCAAGTCCA AGGTCACGGC GCTATGCTGG CATCCAAACA 
     2341 AGGAGGGCTG CTTAGCCTTT GGGACCGATG ACGGAAAAGT GGGATTGTAT GACACCTGCT 
     2401 CCAACAAACC TCCACAGATT TCAAGCACAT ATCATAAGAA GACCGTATAT AGGTTGGCTT 
     2461 GGGGGCCACC AGTGCCCCCC ATGTCACTTG GAGGGGAAGG AGACAGGCCT TCCCTCACCT 
     2521 TGTACAGCTG TGGAGGGGAA GGCATTGTGC TGCAGCATAA TCCCTGGAAG CTCAGTGGAG 
     2581 AGGCCTTTGA CATCAACAAA CTCATGAGAG ACACCAACTC CATTAGATAC AAGCTGCCTG 
     2641 TGCACACAGA GATCAGCTGG AAAGGAGATG GCAAAGTCAT GGCTCTTGGA AATGAAGATG 
     2701 GATCAATAGA AATATTCCAA GTCCCAAACT TGAAACTGCT CTGCACCATC CAGCAGCACC 
     2761 ACAAGCTTGT GAACGCCATA GTCTGGCACC ATGAGCATGG CAGCCGTCCA GAGCTGAGCT 
     2821 GCCTCCTTGC TTCTGGCTCC AACAATGCAG TTATCTACGT GCACAACCTG AAAGCTGTCC 
     2881 TAGAGAGCAA TCCTGAGTCT CCAATAACCA TCACAGAGCC CTACCGGACC CTCTCAGGGC 
     2941 ACACAGCCAA GGTTACCAGT CTGGCATGGA GCCCACATCA TGATGGAAGG CTGGTATCTG 
     3001 CTTGCTACGA TGGCACAGCT CAGGTGTGGG ATGCCCTCCG AGAAGAACCT TTGTTCAATT 
     3061 TCCGAGGACA TCGAGGCCGG CTGTTATGTG TTGCGTGGTC CCCAGTGGAT CCAGAATGCA 
     3121 TCTACTCAGG TGCAGATGAC TTCTGTGTCT ACAGGTGGCT GACCTCCATG CAGGACCATT 
     3181 CCCGGCCTCC TCAAGGGAAA AAATGTATTG AATTAGAGAA AAAACGGCTC TCTCAATTTA 
     3241 AGCCAAAGCT AAAAAAGAAG AAAAAACCTA CCTTGCGACT GCCCATAAAG CAAGATTCAT 
     3301 CCATTGGCAA TGAAGATGAG AGTGTGAGGG AGAACTTGGC ACCTGTTGAG AATGGCTTGT 
     3361 CCGATCAGGA TGGTGAGGAG GAAGCCCAGG AGCCGGAGCT GCCTCCCTCT CCCGTGGTTT 
     3421 GTGGAGAACC TGTTTCCTGC ACTGCTGTCT CCTTAGGCTT GGAAAAGTCA AAAGTCACTG 
     3481 TTACTAGTAA AGTCGCCTCA CTGAAAAAGG AGCCACCTAA AGAGAAGCCA GAAGCCTTGC 
     3541 TCAAGAAGAG GAAGGCTCGC TCCATGCTCC CCCTGAGCAC CAGCTTAGAC CACAGGTCCA 
     3601 AAGAGGAGCT CCATCGAGAC TGTTTGGTTC TAGCAACGGC AACACACGCC AGAGCAGAAC 
     3661 TGCATGAAGA TGTGTCTGCC GACCTTGAGG AACGATTTCA CTTGGGCCTT TTCACAGACA 
     3721 GGGCTGCTCT GTACAGGATG ATGGAGACAG AGGGGAAAGG TCACTTGGAA AGTGGCCACC 
     3781 CCGAGCTTTT TCACCAGCTC ATGCTCTGGA AGGGAGATCT GAAAGGCGTT CTCCAGGCTG 
     3841 CAGCAGAGAG AGGCGAGCTC ACAGACAGTC TCGTGGCCGT GGCACCAGTA GCTGGCTACA 
     3901 GTGTGTGGCT GTGGGCTGTG GAAGCCTTTG CCAAGCAGCT GTGTTTCCAG GATCAGTATG 
     3961 TCAAGGCTGC CTCCTACCTG CTATCCATCC ACAGAGTGTA CGAAGCTGTG GAGCTTCTCA 
     4021 GGTCAAACCA TCTCTACAGA GAAGCTATTG CAGTTGCCAA GGCTCGGCTG CGCCCTGAGG 
     4081 ACCCTGTTCT GAAGGACCTG TACCTCAGCT GGGGGTCCAT CCTGGAGAGA GATGGCCACT 
     4141 ATGCAATTGC AGCCAAGTGC TACTTAGGAG CCACTTCCGC CTATGATGCA GCCAAAGTCC 
     4201 TAGCCAGAAA GGGAGATGCA GCCTCTCTTA GAACAGCTGC AGAGCTGGCT GCCATAGCAG 
     4261 GAGAGAGTGA GCTGGCTGCT TCCCTGGCTC TTAGGTGCGC CCAAGAGCTG CTTCTGCTGA 
     4321 AGAACTGGGT GGGTGCACAG GAAGCCTTGG GACTGCATGA AAGCCTGCAG GGCCAGAGAC 
     4381 TGGTGTTCTG CCTCCTTGAG CTTCTATGCC GGCACCTGGA GGAAAAACAG CCTCCAGAGG 
     4441 TCAGAGGCCC CTCTTCCATT TACCACCAGT GGGCCACTGG TTCTGAGGGA ACCTTGGTGC 
     4501 AGAGAGTAAC TGGTGTGTGG CGCAGCGCCT TCAGTGTGGA CACCCCGGAG CAGTGTCGGG 
     4561 CCGCCTTGCA GAAGCTACAG GATGTCAAGT ACCCATCAGC AACAAGTAAC ACTCCCTTCA 
     4621 GACAGCTGCT GCTTCATGTC TGCCATGACC TAACCTTAGC AATGCTGAGC CAGCAGGCTG 
     4681 CTTCCTGGGA GGAGGCTGTC CCTGCACTGC TGCAGGCTGT GGTTCGGAGC TACACCTCAG 
     4741 GGAATTTCAC CCTCATGCAA GAAATCTACT CAGCCTTTCT CCCAGGCGGC TGTGACCACC 
     4801 TACGAGACAA ACTAGGTGAC CTATCTCCTA CCATGGCGGC TTTCAAAAGC TTAGAGGCCT 
     4861 TTTTCATTTA TGGGCAACTG TATGAAGTCT GGTGGTCACT CTGTGGGCCT GGCTCAGAAT 
     4921 CAAGCATCTG GGTGTGGTCG GCTGAAAGCA CAGTCTCTGA TAAACAGAGC AAGCCAGGGG 
     4981 ACAGTGCCAG TGCTGAAGAC ATGGAGCAGC CTCTAGGCCC AGGCCTGAGG CTCAGTGCGG 
     5041 AGAGTGAACG TTTGCTGAGT GCCTGCAAGG AGCTCTTCTC AGAGAGGCAT GCCAGCCTAC 
     5101 AGACCTCCCA GAGGACTGTT GCTGAAGTCC AAGAGACGCT GGCAGAGATG ATCCGCCAGC 
     5161 ACCAAAAGAG TCAGCTCTGC AAGGCCACAG CCAACGGCCC CAACAGAGAT GAACCCAGCC 
     5221 AGCAAGCAGA GCGGGCCCCT TCCCAACCTC CGAGCCCAAC AGAAGAAAGA AATGCACCGG 
     5281 TGTCTCTCCC TGAATTAACC AGAAGGCTCA CAGAGGCAAA CGAGAGAATA GCTGAGTTTC 
     5341 CAGAGAGTGT AAAGGCCTGG CCCTTTCCAG ATGTGCTGGA GTGCTGTCTC GTCTTACTTC 
     5401 ACATCGGGTC CCAGTGTCCT GGTGCTGTGA ACCCTGAGAT GCAGCAACAG GCCCAGAAGC 
     5461 TCCTTCAGAA GTATGGCCAC ACTAGAGCTT ACAGAAGACA CTGCCAGATT CGACACACAA 
     5521 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5581 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5641 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5701 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5761 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5821 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5881 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5941 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     6001 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     6061 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     6121 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     6181 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     6241 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     6301 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     6361 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     6421 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6481 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6541 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6601 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6661 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6721 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6781 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6841 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6901 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6961 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     7021 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     7081 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     7141 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     7201 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     7261 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     7321 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     7381 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     7441 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7501 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7561 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7621 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7681 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7741 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7801 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7861 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7921 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7981 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     8041 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     8101 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     8161 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     8221 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     8281 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     8341 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     8401 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     8461 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8521 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8581 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8641 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8701 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8761 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8821 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8881 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8941 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     9001 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     9061 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     9121 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     9181 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     9241 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     9301 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     9361 AACGCGAATT TT
//