LOCUS       RR217644                9345 bp    DNA     circular  SYN 
DEFINITION  Iqsec2 (myc-DDK-tagged) - Rat IQ motif and Sec7 domain 2 (Iqsec2), transcript variant 1
ACCESSION   NM_001277425
VERSION     NM_001277425.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5502..5593 
                     /label="Myc-DDK"  
     misc_feature    5623..6208 
                     /label="PolyA signal"  
     misc_feature    5680..5699 
                     /label="Restriction Enzyme"  
     misc_feature    6484..7156 
                     /label="ColE1"  
     misc_feature    7586..8380 
                     /label="Kan/Neo"  
     misc_feature    8415..8717 
                     /label="SV40 ori"  
     misc_feature    8803..9280 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5484..5490 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5484 
                     /label="Iqsec2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCGGGG TCCGGTCCTC CGGGTGGCCC GGGATCTGAG AGCCCCAACC 
     1081 GGGCGGTGGA GTACCTGCTG GAGCTGAACA ACATCATCGA GAGTCAGCAG CAGCTGTTGG 
     1141 AGACCCAGCG GCGGCGCATA GAAGAGCTGG AGGGCCAGTT GGACCAGCTC ACCCAGGAGA 
     1201 ACCGCGACCT GCGGGAGGAG AGCCAGCTGC ACCGTGGGGA ACTGCACCGG GACCCCCTTG 
     1261 GCGCGCGGGA TAGTCCTGGC CGCGAGAGCC AGTACCAGAA CCTGCGTGAG ACCCAGTTCC 
     1321 ACCACCGCGA GCTGCGGGAG AGCCAGTTCC ACCAGGCGTC CCGGGACGTG GGCTACCCGA 
     1381 ACCGGGATGG CGCTTACCAG AACCGGGAAG CTATCTATCG GGATAAGGAG CGAGAAGCTT 
     1441 CCTATCAGCT CCAGGACACT ACCGGTTACA CAGCCCGCGA GCGCGACGTG GCCCAGTGCC 
     1501 ACCTGCACCA TGAGAACCCA GCCCTGGGTC GCGAGCGTGG CGGGAGGGAG GCTGGGCCAG 
     1561 CGCACCCGGG CCGCGAGAAG GAAGCCGGCT ATTCCGCGGC GGTGGGCGTG GGGCAGCGGC 
     1621 CACCGCGGGA GCGGGGCCAG TTGAGCCGTG GCGCATCCAG AAGCTCCAGC CCTGGCGCGG 
     1681 GCGGAGGTCA CAGCACCAGT ACCAGCACCA GCCCTGCTAC AACCCTCCAG AGAAAATCTG 
     1741 ATGGTGAGAA TTCCAGAACA GTCAGTGTGG AGGGTGATGC CCCAGGCAGT GACCTGAGCA 
     1801 CAGCGGTTGA TAGTCCTGGG AGCCAACCCC CCTACCGGCT GAGCCAACTG CCCCCAACCA 
     1861 GCAGCCACAT GGGGGGCCCC CCTGCTGGCG TGGGCCTACC TTGGTCTCAG AGGGCTCGCC 
     1921 TCCAGCCAGC CAGTGTCGCC CTGAGGAAGC AGGAGGAGGA GGAGATAAAG CGCTCCAAGG 
     1981 CCCTGTCAGA CAGCTATGAA CTCTCCACAG ACCTACAGGA CAAGAAGGTG GAAATGCTGG 
     2041 AGAGGAAGTA TGGGGGCTCC TTCCTGAGCC GCAGGGCTGC CAGGACCATC CAGACAGCCT 
     2101 TTCGCCAGTA TCGTATGAAT AAGAACTTTG AGCGACTGCG CAGCTCGGCT TCAGAGAGCC 
     2161 GCATGTCCCG CCGCATCATC TTGTCCAACA TGCGGATGCA GTTCTCCTTT GAGGAGTACG 
     2221 AGAAAGCACA GAACCCTGCA TACTTCGAAG GCAAGCCTGC CTCATTGGAT GAGGGTGCAA 
     2281 TGGCTGGAGC CCGGAGCCAC CGGCTTGAAC GGGGCCTCCC CTATGGAGGC TCCTGTGGTG 
     2341 GGGGCATCGA TGGTGGTGGA AGTTCAGTCA CCACATCTGG AGAGTTTTCT AATGACATCA 
     2401 CAGAGCTCGA GGACTCCTTC TCCAAACAGG TAAAGTCTCT GGCTGAATCC ATTGATGAGG 
     2461 CCTTGAACTG CCACCCATCA GGGCCTATGT CTGAGGAGCC AGGGTCAGCC CAACTGGAGA 
     2521 AGCGGGAATC AAAGGAACAG CAAGAAGACA GCTCAGCCAC ATCCTTCAGT GACCTCCCCC 
     2581 TCTACTTGGA TGACCCAGTT CCTCCTCCAT CACCTGAGCG ACTGCCCAGC ACAGAGCCCC 
     2641 CACCCCAAGG CCGCCCTGAG TTCTGGGCAC CAGCTCCCCT CCCACCAGTT CCTCCACCAA 
     2701 TGCCACCAGG GACCCGGGAA GATGGTAGCC GTGAGGAAGG CACTCGAAGG GGTCCTGGGT 
     2761 GCTTGGAGTG CCGGGATTTC CGGCTCCGGG CTGCACACCT CCCCCTCCTC ACCATTGAGC 
     2821 CTCCCAGTGA CAGTTCTGTA GACCTGAGTG ACCGCTCAGA TCGCGGCTCT GTCCACCGCC 
     2881 AGCTGGTGTA TGAGGCTGAT GGCTGCAGCC CCCATGGGAC CCTGAAGCAC AAGGGGCCAC 
     2941 CAGGCAGGGC CCCAATCCCA CACCGCCACT ACCCTGCCCC TGAGGGTCCA GCTCCAGCCC 
     3001 CACCAGGGCC CCTGCCACCA GCCCCCAATA GTGGTACTGG GCCCAGTGGT GTGGCTGGAG 
     3061 GTCGGAGGTT GGGAAAGTGT GAGGCAGCAG GTGAGAACTC TGATGGTGGA GACAATGAGA 
     3121 GCCTTGAGAG CTCCAGCAAC TCTAATGAGA CCATCAACTG CAGCTCTGGC TCCTCCTCTC 
     3181 GGGATAGCCT GAGGGAACCT CCAGCCACTG GCCTGTGCAA GCAGACTTAC CAGCGGGAGA 
     3241 CAAGGCACAG CTGGGACTCG CCAGCCTTCA ACAATGACGT GGTACAAAGA AGGCATTACA 
     3301 GAATTGGCCT CAACCTCTTC AATAAGAAGC CAGAGAAGGG TATCCAGTAT TTGATCGAGC 
     3361 GAGGCTTCCT GTCAGACACT CCTGTGGGGG TGGCTCACTT CATCCTGGAG CGGAAAGGCC 
     3421 TGAGTCGGCA GATGATAGGG GAATTCCTGG GGAATCGACA GAAGCAGTTC AACAGAGATG 
     3481 TGTTGGACTG TGTGGTGGAT GAGATGGACT TCTCCTCTAT GGATTTGGAC GATGCACTCA 
     3541 GGAAGTTCCA ATCCCATATC CGAGTTCAGG GTGAGGCCCA GAAAGTGGAG CGACTCATTG 
     3601 AAGCTTTCAG CCAGCGGTAC TGTGTCTGTA ACCCTGCCCT CGTACGACAG TTCCGGAACC 
     3661 CAGACACTAT CTTCATCCTT GCTTTTGCCA TCATCCTCCT CAATACCGAT ATGTACAGCC 
     3721 CCAGTGTCAA GGCTGAACGC AAGATGAAGC TGGATGACTT CATTAAGAAC CTGAGAGGAG 
     3781 TTGACAATGG TGAAGACATC CCCCGAGACC TCCTAGTAGG CATCTACCAG CGCATCCAAG 
     3841 GTCGTGAACT AAGGACCAAC GATGACCATG TGTCCCAGGT GCAGGCTGTG GAGCGCATGA 
     3901 TTGTTGGCAA GAAACCGGTC CTGTCGCTCC CTCACCGTCG ACTGGTTTGC TGCTGCCAGC 
     3961 TCTATGAGGT GCCAGATCCA AATCGCCCCC AAAGGCTGGG GCTGCATCAG CGGGAGGTCT 
     4021 TCCTCTTCAA TGACCTCCTT GTGGTCACCA AAATTTTCCA GAAGAAGAAG ATCTTGGTGA 
     4081 CATACAGCTT CCGTCAGTCC TTTCCCCTGG TGGAAATGCA TATGCAGCTC TTCCAGAATT 
     4141 CATATTATCA GTTTGGAATC AAGTTACTAT CTGCGGTACC TGGAGGGGAA CGAAAAGTTC 
     4201 TCATTATCTT TAATGCTCCT AGCCTCCAAG ACAGGCTACG CTTTACCTCT GACCTGCGGG 
     4261 AGTCCATTGC TGAGGTGCAG GAGATGGAGA AGTACCGTGT GGAATCGGAG CTGGAGAAGC 
     4321 AGAAAGGTAT GATGCGGCCT AATGCCTCAC AACCTGGAGG AGCCAAGGAC TCAGTGAATG 
     4381 GGACTCTGGC CCGCAGTAGT CTGGAGGACA CTTATGGGGC AGGCGATGGG CTCAAGCGGG 
     4441 GTGCACTCAG TAGTTCCCTG CGAGACCTCT CTGATGCAGG GAAGCGGGGG CGGCGTAACA 
     4501 GCGTGGGATC GCTGGACAGC ACCATCGAAG GGTCTGTTAT TAGCAGTCCA CGCCCTCACC 
     4561 AGAGGATGCC ACCTCCGCCC CCACCCCCGC CGCCAGAGGA GTACAAGAGC CAGAGGCCAG 
     4621 TCTCCAACTC CTCATCTTTC CTGGGCTCCC TATTTGGAAG CAAGAGAGGC AAAGGGCCCT 
     4681 TCCAGATGCC ACCACCGCCA ACAGGCCAGG CCTCTGCCTC GTCTTCATCT GCTTCCTCCA 
     4741 CCCACCACCA CCATCACCAC CACCACCATG GTCACAGCCA TGGTGGCCTG GGGGTGCTAC 
     4801 CTGATGGGCA GTCCAAGCTC CAGGCCCTGC ATGCCCAGTA TTGCCAAGGA CCGGGCCCTG 
     4861 CCCCACCACC CTCTACAACC CCCCAGCAAC CCCCTCTGCC TCCACCCCCT CAGCAGCCTC 
     4921 CACCCCTGCC CCAGCTGGGC TCCATTCCAC CACCACCTGC CTCAGCCCCA CCTGTGGGGC 
     4981 CACATCGACA CTTCCATGCC CATGGCCCAG TTCCAGGACC CCAGCATTAT ACCTTGGGCC 
     5041 GGCCAGGCAG AGCCCCAAGA CGAGGGGCTG GAGGACACCC TCAGTTTGCT CCACATGGCC 
     5101 GCCACCCCTT GCACCAGCCC ACATCCCCGT TACCCCTGTA CAGTCCTGCT CCACAGCACC 
     5161 CTCCTGCCCA TAAACAGGGC CCCAAGCACT TCATCTTCAG TCACCACCCA CAGATGATGC 
     5221 CAGCAGCAGG TGCAGCTGGG GGCCCTGGGT CCCGGCCACC AGGGGGATCC TACTCCCACC 
     5281 CTCACCACCC CCAATCACCA CTGTCCCCAC ACTCACCCAT CCCACCTCAC CCCTCCTACC 
     5341 CGCCCCTGCC CCCACCCTCA CCTCACACTC CACATTCACC CCTACCACCC ACCTCCCCCC 
     5401 ATGGCCCACT GCACGCCTCT GGGCCCCCTG GCACGGCTAA TCCACCCAGT GCAAACCCCA 
     5461 AGGCCAAGCC AAGCCGGATC AGCACTGTGG TCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5521 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5581 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5641 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5701 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5761 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5821 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5881 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5941 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     6001 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     6061 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     6121 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     6181 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     6241 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     6301 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     6361 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6421 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6481 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6541 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6601 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6661 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6721 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6781 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6841 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6901 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6961 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     7021 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     7081 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     7141 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     7201 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     7261 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     7321 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     7381 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7441 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7501 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7561 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7621 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7681 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7741 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7801 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7861 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7921 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7981 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     8041 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     8101 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     8161 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     8221 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     8281 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     8341 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     8401 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8461 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8521 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8581 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8641 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8701 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8761 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8821 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8881 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8941 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     9001 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     9061 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     9121 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     9181 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     9241 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     9301 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//