LOCUS       RR217636                9285 bp    DNA     circular  SYN 
DEFINITION  Pola1 (myc-DDK-tagged) - Rat polymerase (DNA directed), alpha 1, catalytic subunit (Pola1)
ACCESSION   NM_053479
VERSION     NM_053479.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5442..5533 
                     /label="Myc-DDK"  
     misc_feature    5563..6148 
                     /label="PolyA signal"  
     misc_feature    5620..5639 
                     /label="Restriction Enzyme"  
     misc_feature    6424..7096 
                     /label="ColE1"  
     misc_feature    7526..8320 
                     /label="Kan/Neo"  
     misc_feature    8355..8657 
                     /label="SV40 ori"  
     misc_feature    8743..9220 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5424..5430 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5424 
                     /label="Pola1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCCTGTT CACGGAGACG ACTGCAAACT GGAAACAAGC GCTGTGTCAG 
     1081 ATTCAGGGAG TTTTGTAGCT TCTCGAGCAC GTCGAGAAAA AAAATCAAAG AAAGGACGCC 
     1141 AAGAAGCTTT AGAGCGACTG AAAAAGGCTA AAGCTGGTGA GAAGTACAAG TATGAAGTTG 
     1201 AGGACCTCAC AAGTGTTTAT GAAGAAGTTG ATGAAGAGCA GTATTCAAAA CTGGTTCAGG 
     1261 CACGTCAGGA TGATGACTGG ATCGTGGATG ACGACGGTAT AGGCTATGTG GAAGACGGTA 
     1321 GAGAGATTTT TGATGACGAC CTTGAAGATG ATGCACTTGA CACCTGTGGG GAAGGAAGTG 
     1381 ATGGCAAAGC ACACAGAAAG GACAGGAAGG ATGTAAAGAA ACCTTCAGTG ACAAAACCAA 
     1441 ACAATATCAA GGCAATGTTC ATTGCTAGTG CTGGGAAGAA AACCACAGAT AAAACAGTTG 
     1501 ACTTGTCCAA GGATGATCTG CTAGGTGACA TTCTACAGGA TCTGAACACT GAGACACCTC 
     1561 AAATAGCTCC ACCGCCAGTA TTGATACCAA AGAAAAAAAG ATCTACTGGA GCTTCACCGA 
     1621 ATCCTTTCTC TGTGCACACC GCCACGGCAG TTCCTTCAGG AAAAATTGCT TCCCCTGTCT 
     1681 CCAGAAAGGA GCCTCCATTA ACTCCTGTTC CTCTTAAACG TGCTGAATTT GCTGGCGATC 
     1741 TGGCTCAGCC AGAGTGTCCA GAAGATGAGC AGGAGTCAGG GGTTATAGAG TTTGAAGATG 
     1801 GCGACTTTGA TGAGCCCATG GACACAGAGG AAGTAGATGA GGAGGAGCCT GTGACTGCCA 
     1861 AGATTTGGGA CCAAGAGAGT GAGCCAGTGG AAGGGGTGAA GCATGAGGCA GATCCTGAGA 
     1921 CGGGAACCAC ATCTTTCTTA GACAGTTTTC TCCCAGATGT TTCTTGTTGG GACATTGATC 
     1981 AGAAGGACGA AAACAGTTTC TTACTTCAAG AAGTTCAAGT GGACTCCAAT CATCTCCCGT 
     2041 TGGTAAAAGG GGCAGATGAT GAACAAGTCT TTCAGTTTTA TTGGCTGGAT GCTTATGAAG 
     2101 ATCCATACAA CCAACCAGGT GTGGTTTTTC TGTTTGGCAA AGTATGGGTC GAATCAGCCA 
     2161 AAACCCATGT GAGCTGTTGT GTTATGGTGA AAAATATTGA GCGAACACTC TACTTCCTTC 
     2221 CCCGTGAAAT GAAAATTGAC CTAAACACAG GGAAAGAAAC AGCAACTCCA ATTACAATGA 
     2281 AGGATGTTTA TGAAGAATTT GATTCAAAAA TTTCAGCAAA ATATAAAATA ATGAAGTTCA 
     2341 AGTCCAAGAT AGTGGAAAAG AACTATGCTT TTGAGATACC TGATGTTCCA GAAAAATCTG 
     2401 AGTACTTGGA AGTTAGATAC TCGGCAGAAG TGCCACAGCT TCCACAGAAT TTAAAAGGAG 
     2461 AAACATTTTC TCATGTATTT GGAACTAACA CGTCTAGCTT GGAACTGTTC TTGATGAATA 
     2521 GAAAGATCAA AGGACCCTGT TGGCTTGAAG TAAAAAATCC ACAACTCTTG AATCAACCAA 
     2581 TCAGTTGGTG TAAATTTGAG GCAATGGCTT TGAAACCAGA CTTGGTGAAT GTAATTAAGG 
     2641 ATGTCAGTCC TCCTCCACTT GTGGTGATGT CTTTCAGCAT GAAGACAATG CAAAATGTAC 
     2701 AGAACCATCA ACACGAGATC ATTGCTATGG CAGCGCTGGT TCATCACAAT TTCCCATTAG 
     2761 ATAAAGCACC TCCAAAACCT CCCTTTCAGA CGCATTTCTG TGTTGTGTCT AAACCAAAGG 
     2821 ACTGTATTTT CCCATGCGCT TTCAAAGAAG TCATTAAGAA AAAGAATATG GAGGTTGAGG 
     2881 TTGCTGCAAC AGAAAGAACT TTGCTAGGAT TTTTCCTTGC AAAAGTTCAC AAACTTGACC 
     2941 CTGATATTCT CGTGGGTCAT AATATTTGTG GTTTTGAACT AGAAGTGCTA CTGCAAAGAA 
     3001 TTAATGAGTG CAAGGTTCCT TTCTGGTCCA AGATAGGTCG GCTGAGGAGA TCCAACATGC 
     3061 CAAAACTTGG GAGCCGGAGT GGATTTGGTG AAAGAAATGC TACCTGTGGT CGAATGATAT 
     3121 GTGATGTGGA AATTTCAGTA AAAGAATTGA TTCATTGTAA AAGTTACCAT TTGTCTGAAC 
     3181 TTGTTCAGCA GATTCTGAAA ACTGAGAGGA TTGTAATTCC AACAGAAAAT ATACGAAACA 
     3241 TGTATAGTGA ACCTTCTCAT CTGTTGTACC TGTTGGAACA CATCTGGAAA GATGCCAGAT 
     3301 TCATTTTGCA GATCATGTGT GAGCTAAATG TTCTTCCATT AGCATTGCAG ATCACTAACA 
     3361 TCGCTGGGAA CATTATGTCT AGGACACTGA TGGGTGGACG ATCTGAGCGA AACGAGTTTT 
     3421 TGTTGCTTCA TGCATTTTAT GAAAACAACT ATATTGTGCC TGACAAGCAG ATTTTCAGAA 
     3481 AACCTCAGCA AAAACCGGGA GATGAAGATG AAGAAATTGA TGGGGATACC AATAAATATA 
     3541 AGAAAGGACG CAAGAAAGCA GCTTATGCTG GAGGCTTGGT TTTGGACCCC AAAGTTGGTT 
     3601 TTTATGACAA GTTCATTTTG CTCCTGGATT TCAACAGTTT ATATCCTTCC ATCATTCAGG 
     3661 AATTTAACAT TTGTTTTACA ACAGTGCAAA GAGTTGCTTC AGAGACACTG AAAGCTACAG 
     3721 AGGATGAGGA ACAAGAACAG ATTCCAGAGT TGCCAGATCC AAACTTAGAT ATGGGCATTT 
     3781 TGCCCAGAGA GATCCGGAAA CTGGTAGAGC GGAGAAAACA AGTGAAACAG CTAATGAAAC 
     3841 AGCAAGATTT AAATCCAGAC CTTGTTCTTC AGTATGATAT CCGACAGAAG GCTTTGAAGC 
     3901 TCACAGCGAA CAGTATGTAT GGTTGCCTGG GATTTTCGTA CAGCAGATTT TATGCTAAAC 
     3961 CGCTGGCTGC CTTGGTGACA TACAAAGGAA GGGAGATTTT GATGCACACG AAAGAGATGG 
     4021 TACAGAAGAT GAATCTTGAA GTTATTTATG GAGACACAGA TTCAATTATG ATAAATACCA 
     4081 ATAGCACCAA CCTGGAAGAA GTCTTTAAGT TGGGAAACAA GGTAAAAAAT GAAGTGAATA 
     4141 AATTGTACAA ACTGCTTGAA ATAGACATTG ATGGTGTTTT CAAGTCTCTG CTATTGCTGA 
     4201 AGAAAAAGAA ATATGCTGCT CTAGTTGTCG AACCAACATC CGATGGGAAT TACATCACCA 
     4261 AGCAGGAACT GAAAGGATTG GATATAGTTA GAAGGGACTG GTGTGATCTT GCCAAGGACA 
     4321 CCGGAAACTT TGTCATTGGC CAGATTCTTT CTGATCAAAG TCGGGACACC ATTGTGGAAA 
     4381 ACATTCAGAA GAGGTTAATA GAAATTGGAG AAAATGTGCT AAACGGCAGT GTCCCAGTGA 
     4441 GCCAGTTTGA AATTAACAAG GCATTGACAA AGGATCCCCA GGATTACCCT GATAAAAAAA 
     4501 GTCTACCTCA TGTACATGTT GCACTGTGGA TAAATTCTCA AGGAGGCAGG AAGGTGAAAG 
     4561 CTGGCGATAC AGTGTCATAT GTTATCTGTC AGGATGGGTC AAACCTCCCT GCAACTCAGA 
     4621 GGGCCTATGC ACCCGAGCAA TTGCAGAAAC AGGACAATTT AGCCATCGAC ACCCAGTACT 
     4681 ACTTGGCCCA GCAGATCCAC CCTGTTGTGG CTCGAATCTG TGAGCCAATA GATGGAATTG 
     4741 ATGCTGTACT CATTGCATTA TGGCTAGGAC TTGACTCCAC TCAATTTAGA GTTCATCAGT 
     4801 ATCACAAAGA TGAAGAGAAT GATGCTCTCC TTGGTGGTCC AGCACAGCTA ACTGATGAAG 
     4861 AGAAATACAA GGATTGTGAA AAATTCAAAT GCCTTTGTCC TTCATGTGGA ACTGAAAATA 
     4921 TTTATGATAA TGTCTTTGAA GGCTCGGGAA TGGATATGGA GCCCAGCTTG AATCGTTGCA 
     4981 GTAATATTGA TTGTAAGGCT TCACCAGCGA CCTTTATGGT ACAGTTGAGC AACAAATTGA 
     5041 TCATGGACAT TAGACGTTGC ATTAAAAAGT ACTATGATGG CTGGTTGATA TGTGAGGAGC 
     5101 CAACTTGTCG CAATCGCATT CGTCGTCTTC CCCTTCATTT CTCCCGAAAT GGGCCTCTGT 
     5161 GTCCAGCCTG CATGAAAGCT GTGCTCAGAC CAGAGTATTC TGACAAGTCC CTGTACACCC 
     5221 AGCTGTGCTT TTACCGGTAC ATTTTTGATG CGGACTGTGC ACTGGAAAAA CTTCCTGAAC 
     5281 ACGAGAAAGA TAAATTGAAG AAGCAGTTTT TCACTCCCAG AGTTCTTCAG GACTACAGAA 
     5341 AAGTCAAGAA CATAGCAGAA CACTTCTTGT CCTGGAGTGG CTACTCTGAA GTAAACCTCA 
     5401 GCAAGCTCTT CGCCAATTAT GCTGGGAAGA ACACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5461 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5521 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5581 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5641 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5701 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5761 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5821 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5881 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5941 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     6001 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     6061 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     6121 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     6181 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     6241 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     6301 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6361 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6421 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6481 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6541 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6601 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6661 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6721 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6781 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6841 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6901 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6961 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     7021 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     7081 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     7141 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     7201 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     7261 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     7321 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7381 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7441 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7501 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7561 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7621 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7681 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7741 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7801 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7861 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7921 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7981 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     8041 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     8101 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     8161 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     8221 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     8281 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     8341 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8401 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8461 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8521 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8581 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8641 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8701 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8761 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8821 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8881 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8941 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     9001 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     9061 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     9121 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     9181 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     9241 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//