LOCUS       RR217634                9273 bp    DNA     circular  SYN 
DEFINITION  Myom2 (myc-DDK-tagged) - Rat myomesin 2 (Myom2)
ACCESSION   NM_001169141
VERSION     NM_001169141.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5430..5521 
                     /label="Myc-DDK"  
     misc_feature    5551..6136 
                     /label="PolyA signal"  
     misc_feature    5608..5627 
                     /label="Restriction Enzyme"  
     misc_feature    6412..7084 
                     /label="ColE1"  
     misc_feature    7514..8308 
                     /label="Kan/Neo"  
     misc_feature    8343..8645 
                     /label="SV40 ori"  
     misc_feature    8731..9208 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5412..5418 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5412 
                     /label="Myom2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCCTGGTT GTAGTGCCTT TCTACCAGAA AAGACACAAG CACTTTGACC 
     1081 AGTCATATCG AAACATCCAA ACCAGGTACC TCCTGGACCG ATATGCATCG AAAAAGCAAG 
     1141 CGTCCAGTCA ATCATCATCC CAGAGGTCTC TTACGGAGAG GTCCTCTTCG AAGAGAGCTT 
     1201 CCAGTCAGAG CTCCGCTGAG GGCATGACCT GTAGGCTCTG TGCAAAGCGG ATGAGCGCAT 
     1261 CAGAAGAGGA GGAAGTTGAA AATGAAAACA GGTACCGGTC TCTAGCGGCT TCTTACGGAG 
     1321 AAGCCAAAAG GCAGCGTTTC CTCAGTGAGC TGGCCCAGCT GGAGGAGAAC GTACAATTGG 
     1381 CACGGACACA GGCTCGGAAC AAGCTGGACA AGTACTTCCT GGAGCAGACG GTGGCAGACA 
     1441 ATCTGGCTTT GGAGAGAAGC TCATTTGAAG ACCGGCTGAG CAGGGCCCCT GAGATCCTGG 
     1501 TGAGGTTAAG GTCCCACACC ATCTGGGAGC GGATGTCTGT GAGGCTCTGT TTCACTGTAC 
     1561 AAGGATTCCC CACTCCTGTG GTGCAATGGT ACAAAGACGG CAGTCTGATC TGCCAGGCAG 
     1621 GGGAGCCAGG CAAGTACCGG ATTGAGAGCA GGTACGGAGT GCACACTCTG GAGATCAACA 
     1681 GGGCCGACTT TGAGGACACA GCGACCTATT CAGCAGTAGC AACGAATCCC CATGGACAAG 
     1741 TGTCTACCAA TGCTGCGGTG GTGGTGAGAA GGTACCGAGG AGATGAGGAG CCCTACCACT 
     1801 CAGTAGGACT CCCAATTGGA TTGCCTCTCT CATCTGTGAT CCCGTACACA CATTTTGATG 
     1861 TCCAGTTTTT GGAGAAATTC GGGGTCACCT TCAGAAGAGA GGGCGAGACG GTCACACTCA 
     1921 AGTGCACATT ATTGGTGACA CCAGATCTGA AGAGGGTACA GCCTCGAGCC GAGTGGTACC 
     1981 GGGATGATGT GCTGCTGAAA GAGTCCAAGT GGACCAAGAT GTTCTTCGGA GAAGGCCAGG 
     2041 CCTCACTGTC CTTCAGCCAC CTGAACAAGG ATGATGAGGG GCTTTACACC TTGAGGATCG 
     2101 TGTCCCGGGG TGGAGTCAGT GACCACAGCG CTTTCATGTT TGTCAGAGAT GCTGACCCGC 
     2161 TGGTCACAGG GGCTCCTGGG GCACCCATGG ACCTGCAGTG CCATGATGCC AACCGAGACT 
     2221 ATGTTATCGT GACTTGGAAA CCACCCAACA CCACTACTGA GAGCCCCGTC ATTGGCTACT 
     2281 TCATTGACAA ATGTGAGGTG GGAACTAACA ACTGGGTTCA GTGCAATGAC GCACCGGTAA 
     2341 AGATCTGCAA GTACCCCGTC ACCGGACTTT TCGAAGGAAG ATCGTACGTG TTCCGCGTGA 
     2401 GGGCTGTCAA TAACGCCGGC ATAAGCAGGC CTTCCAGGAT CTCCGATGCT GTGGCTGCCC 
     2461 TTGACCCTGT GGACCTCAGA AGGTTGCAAG CGATTCATTT GGAAGGAGAG AAAGAAATCG 
     2521 TCATCTACCA GGATGACCTT GAAGGTGATG TTCAGATTCC AGGACCTCCC ACCAATGTCC 
     2581 AGGCTTCAGA GGTCAGCAGA AACTACGTTG TCCTCAGCTG GGATCCTCCT TCTCCCCGTG 
     2641 GCAAGGAGCC CTTAATGTAC TTCATTGAGA AGTCGGCAGT TGGGAGTGGC AGCTGGCAGA 
     2701 GGGTCAATGC CCAGACAGCA GTGAGATCTC CTCGGTATGC TGTCTTTGAC TTGGCGGAAG 
     2761 GGAAGTCCTA TGTGTTCCGG GTTTTGTCAG CCAATAAGCA TGGCCTGAGT GACCCATCAG 
     2821 AGATAACACC ACCTATTCAG GCTCAGGACA TGATTGTGGT TCCCTCTGCA CCTGGCCGGG 
     2881 TGCTTGCTTC CAGGAATACA AAGACATCTG TCGTGGTCCA GTGGGACAGA CCAAAGCATG 
     2941 AAGAAGACCT GCTGGGCTAC TATGTGGACT GCTGTGTGGC GGGAACAAAC ATGTGGGAAC 
     3001 CATGTAACCA TAAACCCATT GGATATAACA GGTTCGTGGT ACACGGCTTA ACCACCGGTG 
     3061 AGCAGTACAT CTTCCGGGTT AAGGCTGTGA ATGCCGTGGG GACCAGCGAG AACTCCCAGG 
     3121 AGTCAGAGGT CATCAAGGTC CAGGCAGCCC TCACTGTCCC CTCTCATCCC TACGGGATTA 
     3181 CCCTTCTCAA CTGTGACGGT CACTCCATGA CCCTGGGCTG GAAAGTGCCC AAGTTCAGCG 
     3241 GCGGCTCTGC CATCATAGGT TACTACCTGG ACAAGCGGGA AGTGCATCAT AAGAACTGGC 
     3301 ACGAGATCAA CTCTTCGCCT GTCAAAGAGA GGATCTTAAC GGTGGAAGGT TTGACTGAAG 
     3361 GCTCCTTGTA TGAGTTCAAA ATCGCTGCTA CCAACCTGGC TGGGATCGGG CAGCCATCGG 
     3421 ATCCCAGTGA GCACTTCAAG TGTGAGGCCT GGACAGCCCC AGAACCTGGT CCCGCCTATG 
     3481 ACTTGACGTT CTGTGAGGTC CGAGACACAT CTCTGGTCAT ACTGTGGAAA GCCCCGGTGT 
     3541 ACTCCGGCAG CAGTCCTGTT TCTGGATATT TTGTGGATTT TAAGGAAGAG GATTCTGGGG 
     3601 AGTGGAAGAC CACCAGCGAG GCAGCAACTC CAAATCGCTA CTTAAAGGTC TGTGACCTGC 
     3661 ATCAAGGTAA GACCTATGTA TTCAGAGTTC GAGCAGTGAA TGCCAGTGGA CCAGGGAAGC 
     3721 CGTCAGACAC ATCGGAGCCT GTGCTCGTGG AGGCCCGGCC AGGCACAAAG GAGATCAGTG 
     3781 CTGGTGTGGA TGAGGAGGGC AACATCTACC TTGGGTTTGA CTGCCAGGAA ATGACAGACG 
     3841 CCTCGCAGTT CACCTGGTGC AAGGCCTATG AGGAGATCGC CGATGAGGAG CGGTTCGAGG 
     3901 TTCACACAGA GGGTGATCAC TCAAAGCTGT ACTTCAAGAA TCCAGATAAA ATAGACATAG 
     3961 GGACATATTC TGTATCTGTA AGTGACACGG ATGGTGTGTC TTCCAGCTTC GTTTTGGATG 
     4021 AGGAAGAACT TGAGCGTTTG ATGGCGTTGA GCAATGAGAT AAAGAATCCC ACAATTCCTC 
     4081 TGAAATCAGA ATTAGCATAT GAGATTTTCG ATAAGGGCCA GGTCCGATTC TGGCTCCAGG 
     4141 CAGAGCACCT CTCACCTGAT GCCAACTTCC GGTTCATTAT TAATGACAGA GAAGTTTCTG 
     4201 ACAGTGAGAC ACACAGAATT AAATGCGACA GATCGACAGG CATGATTGAG ATGGTCATGG 
     4261 ATCGGTTTAC TATTGAAAAC GAAGGGACCT ACACTGTGCA GATTCAAGAT GGAAAAGCCA 
     4321 AGAATCAGTC TTCGCTGGTG CTCATTGGAG ATGCATTCAG GGCTGTCCTA GAAGAGGCTG 
     4381 AATTTCAAAG GAAGGAATTT CTACGGAAAC AAGGCCCTCA TTTTGCTGAG TATTTGCACT 
     4441 GGGATGTTAC TGAAGAGTGT GAAGTTCGGC TTATCTGTAA GGTTGCAAAC ACCAAGAGGG 
     4501 AAACCGTTTT CAAATGGCTC AAGGATGATG TCCTTTACGA AACCGAGACA CCACCGCCGG 
     4561 ACCTGGAGAA GGGGATCTGT GAGCTGCTTA TACCCAAGTT GTCTAAGAAG GACCATGGCG 
     4621 AGTACAAAGC AACCTTGAAA GATGACAGAG GTCAAGATGT ATCTGTCTTG GAAGTAGCTG 
     4681 GCAAAGTGTA CGAGGACATG ATCTTGGCAA TGAGCCGAGT GTGTGGAGCC TCTGCCTCAC 
     4741 CCCTGAAGGT TCTCTGCACT CCAGAGGGCA TCCGGCTTCA GTGCTTCATG AAGTATTTCA 
     4801 CAGAGGAAAT GAAGGTCAGC TGGTACCACA AAGAGGCTAA GATCTCATCC AGTGAGCACA 
     4861 TGCGGATCGG AGGAAGCGAG GAGATGGCCT GGCTGCAGAT CTGTGAGCCC ACAGAGAAGG 
     4921 ACAAGGGGAA ATACACTTTC GAGATCTTCG ATGGCAAAGA CAACCACCAA CGCTCACTGG 
     4981 ACCTCTCTGG GCAAGCCTTC GATGAAGCAT ATGCCGAGTT CCAGCAGCTC AAGGCTGCTG 
     5041 CATTTGCTGA GAAGAATCGT GGCAAGGTGA TTGGCGGTTT GCCCGACGTG GTGACCATAA 
     5101 TGGAAGGCAA GACCTTGAAC CTCACCTGCA CAGTATTTGG AAACCCGGAC CCTGAGGTGG 
     5161 TCTGGTTTAA GAACGACAAG GACATTGAGC TAAGCGAGCA CTTCTCGGTG AAGGTGGAGC 
     5221 AGTCCAAGTA CGTCAGCCTG ACCATTAAGG GGGTGACTGC TGAGGACTCG GGCAAGTACA 
     5281 GCATCAACGT GAAGAACAAA TACGGAGGAG AGAAGATCGA TGTCACCGTC AGCGTGTACA 
     5341 AACACGGGGA GAAGATCCCC GACATCTCAG CTCCCCAGCA GGCCAAACCC AAGCTTATCC 
     5401 CAGCTTCCAC CTCAGCTGAT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5461 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5521 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5581 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5641 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5701 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5761 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5821 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5881 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5941 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     6001 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     6061 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     6121 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     6181 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6241 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6301 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6361 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6421 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6481 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6541 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6601 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6661 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6721 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6781 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6841 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6901 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6961 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     7021 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     7081 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     7141 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     7201 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7261 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7321 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7381 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7441 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7501 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7561 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7621 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7681 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7741 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7801 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7861 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7921 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7981 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     8041 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     8101 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     8161 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     8221 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8281 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8341 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8401 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8461 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8521 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8581 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8641 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8701 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8761 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8821 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8881 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8941 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     9001 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     9061 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     9121 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     9181 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9241 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//