LOCUS       RR217630                9243 bp    DNA     circular  SYN 
DEFINITION  Nrk (myc-DDK-tagged) - Rat Nik related kinase (Nrk)
ACCESSION   NM_001191797
VERSION     NM_001191797.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5400..5491 
                     /label="Myc-DDK"  
     misc_feature    5521..6106 
                     /label="PolyA signal"  
     misc_feature    5578..5597 
                     /label="Restriction Enzyme"  
     misc_feature    6382..7054 
                     /label="ColE1"  
     misc_feature    7484..8278 
                     /label="Kan/Neo"  
     misc_feature    8313..8615 
                     /label="SV40 ori"  
     misc_feature    8701..9178 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5382..5388 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5382 
                     /label="Nrk"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGGACCT GGGAGTTGGA GGGACAAGGA GGTGACAGAT CTCGGCCACT 
     1081 TGCCGGATCC AACTGGAATA TTCTCACTAG AGAAGACCAT TGGTCTCGGT ACTTATGGAA 
     1141 GAATCTATTT GGGACTCCAT GAAAAGACGG GTTCTTTCGT GGCTATTAAA GTAATGAATG 
     1201 CCCGGAAGAC TCCTTTGCCT GAAATAGGAA AGCGGGTGAG AGTGAACAAA TACCGAAAGT 
     1261 CTGTCGGATG GAGATACAGC GATGAAGAAG AGGATCTTCG GACTGAACTC AACCTGCTGA 
     1321 GGAAGTACTC TTTCCACAAA AACATTGTGA CCTTCTATGG TGCGTTCTTC AAGCTGAATC 
     1381 CTCCTGGCCA TCAGCACCAA CTTTGGATGG TAATGGAGCT CTGTGCAGCA GGCTCTGTCA 
     1441 CTGATGTAGT GAGGATGACA AGAAATCAGA GTTTAAAAGA AGACTGGATC GCTTACATCT 
     1501 GTCGAGAAAT CCTTCAGGGC TTAGCTCACC TTCATGCGCA CCAAGTAATC CACCGGGACA 
     1561 TCAAAGGTCA GAATGTGCTA CTGACCCAGG ATGCTGAAGT CAAGATAGTG GATTTTGGAG 
     1621 TGAGTGCCCA AGTGAGCAGA ACAAATGGAC GGAGAAATAG CTTCATTGGT ACACCGTACT 
     1681 GGATGGCCCC CGAGGTGATT CATTGTGATG AGGACCCAAG ACGTTCCTAT GATTACAGAG 
     1741 TGACTCTGTG TAACCTTCAG CCCCTGGAAG CCCTTTTTGT TATTCTACGG GAAGCTGCTC 
     1801 CCACAGTCAA ATCCAGTGGA TGGTCCCGTA AGTTCCAGAA TTTCATGGAA AATTGCATGA 
     1861 TAAAAAATTT CCTATTTCGT CCTACATCTG GAAACATGCT TCATCACCCA TTTGTCCACG 
     1921 ATATAAAAAA TGAACGACGT GTTGTGGAGT CATTGACCAA GCACCTTACT GGGATCATCC 
     1981 AAAAGAGAGA GAGAAAAGGA ATACCTCTGG CCTTCGAAGG AGAAGAAGCT GTTAACGAAC 
     2041 AATACATCAC AAGGAGATTC AGAGGACCTT CTTGCACTCC TGAACTTCTG AGAGTGCCAA 
     2101 CCAGCAGCCG ATGCAGACCA CTCAGAGTCC TGCATGGAGA GCCTCCTCAG CCAAGGTGGT 
     2161 TACCTGATGA AGAACAGCCA GAGGACCAAG AGCTTCAGCA ACTGCAGGAG GCAGCCAGGA 
     2221 TGTTCATGCC TCTACATTCT CAGGACAAAA CACTTAAGCC TTTACAAAAG CAGGCTCAGT 
     2281 TACCACAGCA TCTACATGTG GCCCCTCAAG TGCTGATGCC AGTACAGGTT GAAGCAACAG 
     2341 TAGCTACGCC TCTAGCTAAA AAGATTCGGG CACCTCCCCA ACTAAGCAAA GCAGCTTGGG 
     2401 GACTCACGTC ACCACCTAGT CAGGATAACA AAGCTAGTTC TCCTGAGGTA CAGGCCCCCA 
     2461 TACCCAGAGA ACAGCAGGCC CAGAATGATG CCTTAGAAAC GGAACAGCCT GAAGATTTGG 
     2521 ACCAGGTGCC AGAAGAATTT CAGGGGCAAG ACAGGGCAGC TGAGGAGCAA AGGCAGGGCC 
     2581 AGGCCGCTGA ACAAGAGCAG AGGCAGAATG GAGTCCCTGA GCAGCATCCA GAGGAGAATA 
     2641 GGGAACCTGA ACAAGCTGAG GTGCAGGAAG AGGCTGTTGA GCCTCCACAA GCTGAGGTTG 
     2701 AGGAGAAGGA TCCTGAGGTA ATACAAGTAC ATGCCCGGGT GTTCCTGCCT TTACAGTCCC 
     2761 AGAACCATCA TGTGTTCTTG CCTCTTCATC TGGACACACA GCTGCTAATT CCAGTAGGGG 
     2821 AACAAAATGA AGAGGCACCT CGGGCACAGG CCTGGGATCT GGAGGCCTCA AGGGCAGCTG 
     2881 GTGCAGTACA AGCACTGGTA GAGGGACTAT CAAGAGACTT GCTTCGAGCA CCAAACGCAT 
     2941 TTACCTCAAA ACCACTTGGT CCACTGCAAA TCTTGTTGGA AAGTTTGTGC ACCAACGGGT 
     3001 TTTACACTGA ACCTGAAGCA GTACAGAAGA AAAAGTCAAG AGTTTCTAGC CTGAAGAAAG 
     3061 CAATAGTAAA AAGACTAACA CCCAAGAGGT TCAGGGCAAA GGCATTATGG AGACGTCAAG 
     3121 ATTTTGAATG CTCAGATGTA GAAAGCAGCA GGAGACGGCG CCAGCGTAGA TGGGAGGAAA 
     3181 TCTTTAATCA GCATGAAGAG CAACTGAGGG AAGTTGAAAA TGATAGAGAC GATGATGACT 
     3241 CATCAGACAA TGATGAAGTG TTTCATTCAA TTCAGGCTGA AGTCCAAATA GAACCATACG 
     3301 CTCCAAATCG TACAGGAAAT GAGGTCCAAG AGAGATCTGC TCCTATGCCT TGCAACCGGA 
     3361 ATCGTACACA TCGTGTGAAA TTTTCTCCAA GTGTCTGTGA GCAGGCTCTT TTGGAAGAAG 
     3421 CTCAGCAGCC GATGGACATC AGACCGAGGA ACTCCCTAAA TCCTCGGAAT TTTCAAGCAG 
     3481 AATCAGAGTC ATCTTCTGAG GAGGACAGCC CTGTGACTAG GAGGAAGTCC CAGTCATCGC 
     3541 CGCCTTATTC TACTATTGAT CAGAGGTTAC TGATTGATAT CCATGTTCCA GATGGATTTA 
     3601 AAGTGGGAAA AATATCACCC CCTGTGTACT TGACAAACGA ATGGGTAGGC TGTAACCCAC 
     3661 TCTCTGAAGT CTTCTGGGAT GATTGGGTAA ACCCTACACC TGCCACTAGG CGACATGAAG 
     3721 AAGATGGTGA TTATGTTGAA CTCTATGATG CTGATGCTAA TGGTGATGGA CGAGCTTCAA 
     3781 ATTATGCTTA TGAAGAAAAC TATGCCAGTG GCCATGATGA CATGAATCAT CAGGCAAATG 
     3841 GCTATAGAGG CCATGGTAAC CGTCACTACA GTGATGGGTC TTTTCGAAAC AGAGGTGATA 
     3901 GTCGTGGTGC TGCTTTCAAT GGGCCAAGGG CAAGTTATTG CAGAGGGGGA ATCTTGAAGA 
     3961 ATGCTGGTAA TGATGGCCGT GATCTAAGGC GAGGGGCTTT CAGAGCCTAT GGAGACAATG 
     4021 AAGCCAGCAA TTACCATGGA GCTGCCAGTG GAGCAAGCGC AGCCTTTGGA AGCCAGGAAG 
     4081 GACATGAAGG CCACATGGGC GGTCGACGAG GTAACAGATA CAGGGAAGTC AATGGAAGCA 
     4141 GCGGAGAGAA TGGCGCATGT GGATTCCATA GAGATGGGGC AGGACTGGAT CAACAACATG 
     4201 TATTCCCAGA TTTTGAAAAT GAACAAGAGC AATCAGATGC TGGAACTGAG ACGTCAGATT 
     4261 CAATTGCCCT GGAGATCACA TCCTTTTCCC TTGATGGTGA GCAAAACAAT GATAGAGCAG 
     4321 AACCAACATT GGGTTTTCCT CTCAGACGCT CATCTTCCAG AGGTTCTGCT TCGGATACTG 
     4381 ACATTATGAG TGACTCATAT AACAAACCAA TAATCCATGC GTATGAAAGG GAATTCTCGC 
     4441 ATGAAATCTT CTGTGGTTCT CTGTGGGGAG TCAATTTGTT GCTGGGCACC AAATCTCATC 
     4501 TGTATCTGAT GGACAGAAGT GGAAAGGCAG AAATCATTAA ACTTGTAAAA CGAAGACCAT 
     4561 TCCGCCAGAT TCAAGTCGTG GAGCAGCTCA ATTTGCTGAT TACCATCTCT GGCCGCAAGA 
     4621 ACAGACTTCG CGTGTATCAT CTGACTTGGC TGAGGAACAA GATTCTGAAT AATGATCCAG 
     4681 AAAGTAAGAA AAGGCAGAAA GCAATGCTGA AGAGAGAGAA AGCCTGCAAA TCTCTGGATA 
     4741 AGTTGATTGG CTGTGAACAC TTCAGTATCC TTCATCATGA AGAAACAACA TACATTGCAA 
     4801 TTGCTTTGAA ATCATCAATT CACCTGTATG CGTGGGCACC AAAATCCTTC AATGAAAGCA 
     4861 CTGCCATTAA AGTGTTTCCA ACTCATGATC TTAAGCCAGT GACAGTTGAC CTGGCTGTTG 
     4921 GTTCTGAGAA AACACTGAAG ATTTTCTTCA GCTCAGCTAA TGGATACCAC ATCATCGATG 
     4981 CAGAATCTGA AGTTATGTCT GATGTGACCT TGCCAAGTAA TAATGTCATC CTTTTACCTG 
     5041 ATTCCTTGGG AGTTGGAGTG ATGCTGTCCT TCAATGATGA AACTGCCCCT GAGGAAGCAA 
     5101 ATGAACAGCT TCTCAAGAAG ATCCTGGACG TGTGGAAAGA CATACCGTCT TCTGCGGCTT 
     5161 TTGAGTGTAC ACAGAGAATC ACTGGATGGG ACCAAAAGGC TGTTGAAGTG CGCTCTCTGC 
     5221 AGGCAACTAT TCTTGAGAAT GAGCTGAAGC GCCGCTCAAT CAAGAAGCTG AGATTTCTGT 
     5281 GTTCTCGTGG GGACAAGCTG TTCTTTGCTT CTCTACTGAA CAATGAGCAC AGCCTGGTTT 
     5341 ACTATGTGAG CATTGCAAAA CTTGAAGCGC TCCAAAGAAG ATATGCTGTT ACGCGTACGC 
     5401 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5461 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5521 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5581 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5641 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5701 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5761 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5821 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5881 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5941 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     6001 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     6061 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     6121 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     6181 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     6241 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6301 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6361 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6421 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6481 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6541 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6601 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6661 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6721 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6781 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6841 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6901 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6961 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     7021 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     7081 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     7141 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     7201 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     7261 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7321 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7381 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7441 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7501 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7561 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7621 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7681 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7741 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7801 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7861 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7921 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7981 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     8041 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     8101 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     8161 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     8221 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     8281 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8341 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8401 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8461 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8521 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8581 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8641 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8701 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8761 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8821 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8881 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8941 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     9001 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     9061 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     9121 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     9181 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     9241 TTT
//