LOCUS       RR217627                9228 bp    DNA     circular  SYN 
DEFINITION  Ptpru (myc-DDK-tagged) - Rat protein tyrosine phosphatase, receptor type, U (Ptpru)
ACCESSION   NM_001191575
VERSION     NM_001191575.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5383..5474 
                     /label="Myc-DDK"  
     misc_feature    5504..6089 
                     /label="PolyA signal"  
     misc_feature    5561..5580 
                     /label="Restriction Enzyme"  
     misc_feature    6365..7037 
                     /label="ColE1"  
     misc_feature    7467..8261 
                     /label="Kan/Neo"  
     misc_feature    8296..8598 
                     /label="SV40 ori"  
     misc_feature    8684..9161 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5358..5365 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5358 
                     /label="Ptpru"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCGAGCT CAGGCTCTGG TCCTGGCGCT CACCTTCCAG TTCTGCGCGC 
     1081 CCGAGACCGA GACTCCCGCA GCTGGCTGCA CCTTCGAGGA GGCGAGTGAC CCGGTTGTGC 
     1141 CCTGCGAGTT CAGCCAGGCT CAGTATGATG ACTTCCAGTG GGAGCAAGTA CGGATCCACC 
     1201 CTGGCACCCG GACCCCTGCA GACCTGCCCC ATGGTGCCTA CTTGATGGTT AATGCTTCCC 
     1261 AGCATGCTCC AGGCCAGAGG GCCCATATCA TCTTCCAGAC CCTGAGCGAG AACGACACCC 
     1321 ACTGTGTGCA GTTCAGCTAC TTCCTGTTTA GCCGGGATGG GCACAGCCCA GGCACCCTGG 
     1381 GCGTCTATGT GCGTGTGAAC GGGGGCCCTC TGGGCAGTGC CGTGTGGAAT ATGACTGGAT 
     1441 CCCACGGCCG TCAGTGGCAT CAGGCTGAAC TGGCTGTCAG CACCTTCTGG CCCAATGAGT 
     1501 ATCAGGTGCT GTTTGAGGCT CTCATCTCCC CAGACCATAA GGGCTACATA GGCTTAGATG 
     1561 ACATCTTGCT ATTCAGCCAT CCCTGCGCGA AGGCCCCTCA CTTCTCCCGC CTTGGAGACG 
     1621 TGGAGGTCAA TGCGGGCCAG AATGCATCCT TCCAATGCAT GGCAGCAGGC AGAGCTGCAG 
     1681 AGGCAGAGCG TTTCTTCCTG CAGCGGCAGA GTGGGGTGCT AGTGCCTGCG GCTGGGGTGC 
     1741 GGCACATCAG TCACCGTCGC TTCCTGGCCA CTTTTCCACT GGCCTCTGTA GGCCGCTCGG 
     1801 AGCAGGATCT GTACCGTTGC GTGTCCCAGG CCCCGCGCGG TGCTGGAGTC TCCAACTTCG 
     1861 CAGAGCTCAT CGTCAAAGAG CCTCCCACCC CCATCGCGCC CCCACAGCTG CTGCGTGCAG 
     1921 GCCCCACCTA CCTCATCATC CAGCTCAACA CCAACTCCAT CATTGGCGAC GGGCCGATCG 
     1981 TGCGCAAGGA GATCGAGTAC CGAATGGCAC GGGGCCCGTG GGCCGAGGTT CACGCTGTCA 
     2041 ACCTGCAGAC CTACAAGCTG TGGCATCTAG ACCCGGACAC TGAGTATGAG ATCAGCGTGC 
     2101 TGCTTACCCG CCCGGGAGAT GGAGGCACAG GCCGCCCAGG GCCACCACTG CTCAGCCGGA 
     2161 CCAAGTGCGC AGAGCCCACG AGGGCCCCCA AGGGCCTGGC TTTTGCTGAG ATCCAGGCTC 
     2221 GTCAGCTGAC CCTGCAGTGG GAGCCCCTGG GCTACAATGT CACGCGTTGT CATACCTATG 
     2281 CTGTGTCCCT TTGTTATCAC TACACCCTGG GCGGCAGCCA CAACCAGACC ATCCGGGAGT 
     2341 GTGTGAAGAT GGAGCGGGGC GCCAGCCGCT ACACCATCAA GAATCTGCTG CCGTTCAGGA 
     2401 ACGTCCACGT GCGTCTGATT CTCACAAACC CTGAGGGGCG CAAGGAGGGC AAGGAGGTCA 
     2461 CCTTCCAGAC AGATGAAGAT GTGCCTGGTG GGATCGCCGC TGAGTCTCTC ACCTTCACCC 
     2521 CACTAGAGGA CATGATCTTT CTCAAGTGGG AGGAGCCCCA GGAACCCAAT GGCCTCATCA 
     2581 CTCAGTATGA GATCAGCTAC CAAAGCATTG AGTCCTCAGA CCCAGCGGTG AACGTGCCCG 
     2641 GCCCGAGACG CACCATCTCC AAACTCCGAA ATGAGACTTA CCATGTCTTC TCCAACCTGC 
     2701 AACCCGGCAC CACATACCTG TTCTCTGTGC GTGCTCGTAC AAGCAAGGGC TTTGGCCAGG 
     2761 CGGCTCTCAC CGAGATAACC ACCAACATCT CAGCACCCAG CTTTGATTAT GCCGACATGC 
     2821 CGTCACCCCT GGGCGAGTCT GAGAACACCA TCACTGTGCT GTTGAGGCCG GCCCAGGGCC 
     2881 GAGGAGCACC CATCAGCGTC TACCAGGTGG TTGTGGAGGA AGAGCGGCCA CGGCGCTTGC 
     2941 GGCGCGAGCC TGGAGGTCAG GACTGCTTCT CGGTACCTCT GACCTTTGAG ACGGCCCTGG 
     3001 CTCGCGGCCT GGTGCACTAC TTTGGGGCTG AACTGGCTGC CAGCAGCCTG CTTGAGGCCA 
     3061 TGCCCTTCAC TGTGGGTGAC AACCAGACCT ATCGTGGCTT CTGGAACCCA CCGCTTGAGC 
     3121 CCAGGAAGGC CTACCTCATC TATTTCCAGG CAGCGAGCCA CCTGAAAGGG GAAACCCGAC 
     3181 TGAACTGCAT CCGAATTGCC AGGAAAGCTG CGTGCAAGGA GAGCAAGCGA CCCCTCGAAG 
     3241 TGTCCCAGAG GTCGGAGGAG ATGGGGCTCA TCCTGGGTAT CTGTGCAGGT GGCCTTGCCG 
     3301 TCCTCATTCT CCTCCTGGGC GCCATCATTA TCATCATCCG TAAAGGGAGG GACCGCTATG 
     3361 CCTACTCTTA CTACCCGAAG CCAGTGAACA TGGCAAAAGC CACGGTCAAC TATCGCCAGG 
     3421 AGAAGACCCA CATGATGACT GCTGTGGACC GTAGCTTCAC AGATCAGAGC ACTCTGCAGG 
     3481 AGGATGAGCG GCTGGGCCTG TCCTTCATGG ATGCTCCTGG CTACAGCCCT CGGGGAGACC 
     3541 AGAGAAGCAG TGGGGTCACT GAGGCCAGTA GCCTCCTGGG GGGCTCTCCT CGGCGCCCCT 
     3601 GTGGCCGGAA GGGCTCCCCG TATCACACAG GGCAGCTTCA CCCTGCAGTA CGAGTGGCTG 
     3661 ACCTGCTGCA GCACATCAAC CAGATGAAGA CAGCCGAGGG CTACGGCTTC AAGCAAGAGT 
     3721 ACGAGAGTTT CTTTGAAGGC TGGGATGCCA CCAAGAAGAA AGACAAGCTG AAGAGCGGCC 
     3781 GGCAGGAGCC AGTGTCTGCC TATGACCGAC ACCGAGTGAA GCTGCACCCG ATGCTGGGAG 
     3841 ACCCTGATGC CGACTACATC TCTGCCAACT ACATAGACGG CTACCACAGG TCAAACCACT 
     3901 TCATAGCCAC TCAAGGGCCA AAGCCTGAGA TGATCTACGA CTTCTGGCGC ATGGTGTGGC 
     3961 AGGAGCAGTG TGTGAGCGTC GTCATGATCA GCAAGCTGGT AGAGGTGGGC AGGGTAAAGT 
     4021 GCTCTCGATA CTGGCCCGAG GACTCAGACA TGTATGGGGA CATCAAGATC ACGCTGGTAA 
     4081 AGACAGAGAC ACTGGCTGAG TACGTGGTGC GCACCTTTGC CCTGGAGCGG AGAGGTTACT 
     4141 CAGCCCGGCA TGAGGTCCGC CAGTTCCACT TCACAGCGTG GCCAGAGCAT GGTGTTCCCT 
     4201 ACCATGCCAC GGGGCTGCTG GCCTTCATCC GGCGCGTGAA GGCTTCCACT CCACCTGATG 
     4261 CTGGGCCTGT TGTCATTCAC TGCAGTGCAG GCACTGGCCG CACAGGCTGC TACATCGTCT 
     4321 TGGATGTGAT GCTGGACATG GCTGAGTGTG AGGGGGTCGT GGACATTTAC AACTGCGTGA 
     4381 AGACCCTCTG TTCCCGGCGG GTCAACATGA TCCAGACCGA GGAACAATAC ATCTTCATCC 
     4441 ACGATGCGAT TCTGGAGGCC TGCCTGTGTG GGGAGACCAC CATCCCTGTC AATGAGTTCA 
     4501 AGGCCACCTA TAGGGAGATG ATCCGCATTG ACCCTCAGAG TAACTCCTCC CAGCTTCGGG 
     4561 AAGAGTTCCA GACACTGAAC TCGGTCACAC CGCCGCTGGA CGTGGAGGAG TGTAGCATTG 
     4621 CCCTGCTGCC CCGGAATCGA GACAAGAACC GTAGCATGGA CGTGCTGCCG CCAGACCGCT 
     4681 GCCTGCCCTT CCTCATCTCC AGTGATGGGG ACCCCAACAA CTACATCAAT GCAGCCCTAA 
     4741 CTGATAGCTA CACACGGAGC GCCGCCTTCA TCGTGACCCT GCACCCACTG CAGAGTACCA 
     4801 CACCCGACTT CTGGCGGCTG GTCTACGACT ATGGGTGCAC CTCCATTGTG ATGCTCAACC 
     4861 AGCTTAACCA GTCCAACTCC GCCTGGCCCT GCCTGCAGTA CTGGCCAGAA CCAGGCAGAC 
     4921 AGCAGTATGG CCTCATGGAG GTGGAGTTTG TGTCTGGCAC AGCAAACGAG GATCTGGTGT 
     4981 CCCGAGTGTT CCGGGTGCAG AACTCTTCAA GGCTGCAGGA GGGCCACCTG CTGGTTCGTC 
     5041 ACTTTCAGTT CCTGCGTTGG TCTGCTTATC GGGACACGCC TGACTCCAGG AAGGCCTTCC 
     5101 TGCACCTGTT GGCTGAGGTG GACAAGTGGC AGGCGGAGAG TGGAGATGGG CGCACCGTGG 
     5161 TGCATTGTCT CAATGGCGGT GGCCGCAGTG GCACCTTCTG CGCCTGTGCA ACGGTCTTGG 
     5221 AAATGATCCG CTGTCACAGC CTGGTGGATG TTTTCTTTGC TGCCAAAACG CTTCGGAACT 
     5281 ACAAGCCCAA TATGGTGGAG ACCATGGATC AATACCATTT CTGCTACGAT GTGGCCCTGG 
     5341 AGTACCTGGA GGCTCTGGAG TTGAGAAGCG GACCGACGCG TACGCGGCCG CTCGAGCAGA 
     5401 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5461 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5521 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5581 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5641 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5701 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5761 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5821 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5881 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5941 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     6001 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     6061 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     6121 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     6181 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     6241 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     6301 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     6361 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6421 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6481 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6541 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6601 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6661 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6721 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6781 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6841 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6901 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6961 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     7021 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     7081 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     7141 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     7201 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     7261 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     7321 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     7381 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7441 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7501 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7561 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7621 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7681 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7741 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7801 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7861 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7921 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7981 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     8041 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     8101 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     8161 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     8221 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     8281 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     8341 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     8401 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8461 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8521 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8581 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8641 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8701 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8761 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8821 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8881 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8941 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     9001 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     9061 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     9121 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     9181 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//