LOCUS       RR217621                9162 bp    DNA     circular  SYN 
DEFINITION  Ankrd50 (myc-DDK-tagged) - Rat ankyrin repeat domain 50 (Ankrd50)
ACCESSION   NM_001191606
VERSION     NM_001191606.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5319..5410 
                     /label="Myc-DDK"  
     misc_feature    5440..6025 
                     /label="PolyA signal"  
     misc_feature    5497..5516 
                     /label="Restriction Enzyme"  
     misc_feature    6301..6973 
                     /label="ColE1"  
     misc_feature    7403..8197 
                     /label="Kan/Neo"  
     misc_feature    8232..8534 
                     /label="SV40 ori"  
     misc_feature    8620..9097 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5301..5307 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5301 
                     /label="Ankrd50"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTAATCCT TGGGAAGAGA AAGTCTGCAA AATGGCCCAG ACGAGTCTCC 
     1081 TGCAAGGGAA GCAGTTCTAC TGTCGGGAAT GGGTTTTCCA CAAACTTCAG CACTGCCTCC 
     1141 AGGAGAAATC AAGCCGCTGT TTCAGTGCAG CCAATGCACA GTCCCTGGTC GGGGATGCTG 
     1201 GGAATAATGC TAGTGCCCTC TCTGGAAAGG GAGCCTCCTG GGGCGTGTTG CTGGTCGGAG 
     1261 GGCCTGGCAG TGGGAAGACA GCCCTGTGCA CTGAGCTCCT GTGGCCCAGT TCCCCCGCCA 
     1321 GCCTGCAGAG AGGTCTGCAT CGGCAGGCTT TGGCTTTCCA CTTCTGCAAA GCCCAGGACT 
     1381 CGGACACACT GTGTGTCGGG GGGTTTATCA GAGGTCTGGT AGCCCAGATC TGCCAAAGCG 
     1441 GACTCCTCCA AGGTTATGAG GACAAGCTAA GAGATCCAGC CGTCCAAAGT CTCCTAGAGC 
     1501 CCGGAGAGTG TGAGAGGAAC CCAGCGGAAG CATTTAAAAG GTGTGTGCTG CTCCCACTCT 
     1561 TGGGGATGAA GCCACCCCCG CAAAGCCTCT ACCTGTTGGT TGACTCCGTG GATGAAGGCT 
     1621 GCAATGTGGT GGAAGGGGAG CAGACGTCAC CCAACCTCTC CGGGACTGTG GCAGAGCTCT 
     1681 TGGCTGGTCA CCACGAGTTC TTTCCCCCAT GGCTCCTGCT TCTCTGCTCT GCCCGGAAGC 
     1741 AGAGCAGAGC TGTCACTAAA ATGTTTACTG GGTTCCGGAA GATCAGTTTG GATGACCTCC 
     1801 GGAAGGCGTA TATCGTCAAG GATGTACAGC AGTACATCCT CCACCGCCTG GATCAAGAGG 
     1861 AGGCACTCCG GCAGCATCTC ACCAAGGAGA CGGCTGAGAC TCTGAACCAG CTGCACATCA 
     1921 AGAGCAGCGG GTGCTTCTTG TACCTGGAGC GAGTGCTAGA TGGAGTTGTA GAGAATTTCA 
     1981 TCATGCTTCG AGAGATCCGT GACATCCCCG GAACTCTGAA TGGTCTCTAC CTCTGGCTCT 
     2041 GCCAGAGACT TTTTGTCCGA AAACAGTTTG CCAAAGTTCA GCCCATTCTG AATGTGATCC 
     2101 TCGCTGCCTG CCGGCCCCTG ACCATGACAG AATTATACCA TGCTGTGTGG ACCAAAAATA 
     2161 TGTCTCTAAC CTTGGAAGAC TTTCAGCGCA AGCTAGACGT CCTCTCCAAG CTCTTGGTGG 
     2221 ATGGGTTGGG CAGCACCAAA ATCCTGTTTC ACCACAGCTT TGCTGAGTGG CTCTTGGATG 
     2281 TGAAGCACTG CACCCAGAAA TACCTGTGTA ACGCTGCAGA AGGACACAGG ATGCTGGCCA 
     2341 TGAGCTACAC CTGCCAGGCC CGGAACCTGA CCCCACTGGA AGCACAAGAA TTTGCCTTGC 
     2401 ACTTGATTAA TTCAAACTTG CAGCTGGAGC CAGCCGAACT GGCTCTGTGG ATGATATGGA 
     2461 ATGGTACCCC AGTCAAAGAC TCACTGTCCA CCTTGATACC CAAGGAGCAA GAGGTCCTGC 
     2521 AGCTGCTGAT CCGAGCTGGT GCTCATGTCA ACAGTGAGGA TGATCGCACC TCCTGTATTG 
     2581 TCCGGCAAGC CCTCGAGAGA GAGGACTCAA TTCGGACTTT ACTGGATAGT GGAGCTTCTG 
     2641 TCAATCAGTG TGATTCCAAT GGGAGAACTC TGCTGGCCAA TGCCGCATAC AGTGGCAGTC 
     2701 TGGATGTTGT GAACTTGCTT GTCTCCAGGG GAGCCGATTT AGAAATCGAA GACACCCATG 
     2761 GACACACGCC ACTTACTCTA GCTGCTAGAC AAGGGCACAC AAAGGTGGTT AACTGTCTAA 
     2821 TTGGGTGTGG AGCCAATATT AATCATACAG ATCAGGACGG TTGGACAGCA CTGAGGTCTG 
     2881 CTGCTTGGGG GGGCCACACA GAAGTGGTGT CTGCTCTGCT TTACGCTGGC GTAAAGGTAG 
     2941 ACTGTGCAGA TGCGGACAGC CGGACTGCTC TGCGTGCAGC AGCCTGGGGT GGCCACGAGG 
     3001 ACATCGTCCT GAACCTGCTA CAACATGGTG CAGAGGTCAA CAAAGCCGAC AATGAAGGGA 
     3061 GGACTGCACT CATAGCTGCA GCATATATGG GCCACAGAGA GATCGTAGAG CACCTGCTGG 
     3121 ACCATGGTGC AGAGGTAAAC CATGAGGACG TGGATGGCAG AACAGCGCTC TCTGTGGCTG 
     3181 CGCTCTGTGT GCCGGCAAGC AAAGGGCATG CGTCAGTCGT GAGCCTCTTG ATTGATCGAG 
     3241 GGGCTGAGGT AGATCACTGT GATAAGGATG GCATGACCCC CCTGCTGGTC GCTGCCTACG 
     3301 AAGGTCATGT GGATGTTGTA GACCTGCTTC TAGAAGGAGG AGCAGATGTT GATCACACCG 
     3361 ATAACAATGG ACGGACACCC CTTCTGGCTG CAGCTTCCAT GGGGCATGCT TCCGTGGTCA 
     3421 ACACACTTCT GTTCTGGGGC GCAGCCGTGG ACAGCATTGA CAGCGAAGGC AGGACAGTTC 
     3481 TCAGCATAGC TTCAGCACAG GGGAATGTGG AGGTGGTGCG CACTCTCCTG GACAGAGGCT 
     3541 TGGACGAGAG CCACCGAGAT GATGCTGGCT GGACACCTCT ACACATGGCA GCTTTTGAGG 
     3601 GTCACAGGTT AATATGTGAA GCTCTCATTG AACAAGGTGC TAGAACAAAT GAGATTGACA 
     3661 ATGATGGGCG CATCCCCTTC ATTTTGGCCT CCCAAGAGGG CCATTATGAT TGTGTCCAGA 
     3721 TTCTGCTGGA GAACAAATCC AACATTGATC AGAGAGGCTA TGACGGGAGA AATGCATTAC 
     3781 GAGTTGCTGC CCTGGAAGGA CACCGGGACA TCGTGGAATT ACTCTTTAGC CATGGGGCTG 
     3841 ATGTGAACTA CAAAGATGCC GATGGGCGGC CCACACTTTA CATATTGGCC TTAGAAAACC 
     3901 AGCTTACAAT GGCCGAGTAC TTTCTAGAAA ATGGTGCAAA CGTGGAGGCC AGTGATGCAG 
     3961 AGGGAAGGAC AGCGCTGCAC GTCTCCTGCT GGCAGGGTCA CGTGGAGATG GTACGGGTAC 
     4021 TTATCGCCTG CCATGCCGAT GTCAATGCAG CAGACAATGA GAAGCGCTCA GCCTTGCAGT 
     4081 CTGCGGCCTG GCAGGGCCAT GTCAAAGTGG TCCAGCTTCT GATAGAGCAT GGTGCCGTGG 
     4141 TGGACCACAC GTGCAACCAA GGAGCCACAG CCCTCTGCAT CGCAGCCCAG GAGGGACATG 
     4201 TAGACGTAGT GCAGGTTTTG CTGGAGCACG GTGCTGATCC CAATCATGCC GACCAATTTG 
     4261 GACGTACTGC CATGAGGGTG GCAGCCAAAA ACGGGCATTC TCAGATCATT AAGTTGTTAG 
     4321 AAAAATACGG TGCTTCCAGT CTGAATGGCT GCTCCCCTTC CCCTGTCCAC ACAATGGAGC 
     4381 AGAAGCCTCC ACAGTCGACA CCATCCAAGA TGCAGTCTCT TACCATCAGG TCCAACAGCT 
     4441 CTGGTGGTAC AGGCGGAGGG GATCTGCAGC CTTCCCTCCG GGGCTTGCCC AATGGGCCGG 
     4501 CTCATGCCTT CAGCTCTCCA TCAGAGTCTC CAGACTCAAC CGTGGATCGG CAAAAGTCGT 
     4561 CTCTGTCCAA CAATTCTCTG AAAAGCTCCA AAAACTCATC TCTGAGAACA ACGTCCTCCA 
     4621 CAGCCACGGC TCAGACGGTG CCCATTGACA GTTTCCATAG CCTGTCGTTC ACGGAGCAGA 
     4681 TCCAACAGCA CTCGCTGCCT CGCAGCAGGA GCAGGCAGTC TGTTGTGTCC CCCTCTTCCA 
     4741 CAACCCAGTC CTTAGGACAC AGCCATAATT CTCCCAGTAG TGAGTTCGAA TGGAGCCAGG 
     4801 TGAAACCAAG TCTGAAGTCA ACAAAATCAA ATAAAGGAGG CAAGTCAGAA AACTCCAGCA 
     4861 AGTCTGGGTC AGCTGGGAAG AAAGCTAAAC AAAACAATTC CTCACAGCCA AAGGTTCTAG 
     4921 AATATGAAAT GACTCAGTTT GACAAAAGAG GACCTGTTGC CAAGTCTGGG AGTAGCCCGC 
     4981 CTAAGCAGAT GCCAGCTGAA TCTCAGTGTA AAATCATGGT CCCTTCATCT CAGGACAGCA 
     5041 ACCGTGCCCA GCCACAGTTT CTCATCCACC AGCAGGGCGG CGAGCAGAAG AGGAGGAATG 
     5101 GGATAATGAC CAACCCCAAT TACCATCTGC AGAGCAACCA GGTGTTCCTG GGCAGGGTCT 
     5161 CCGTCCCTCG AACACTGCAG GAGCGAGGGC ATCAGGAGGT GCTGGAGGGC TTCCCCCCCT 
     5221 CCGAGACAGA GCTGAACCTT AAACAAGCGC TGAAGCTCCA GATTGAGGGC TCTGACCCCA 
     5281 GCTTCAACTA CAAGAAGGAA ACGCCCTTGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5341 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5401 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5461 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5521 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5581 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5641 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5701 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5761 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5821 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5881 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5941 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     6001 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     6061 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     6121 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     6181 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     6241 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6301 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6361 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6421 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6481 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6541 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6601 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6661 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6721 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6781 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6841 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6901 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6961 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     7021 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     7081 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     7141 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     7201 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     7261 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7321 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7381 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7441 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7501 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7561 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7621 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7681 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7741 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7801 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7861 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7921 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7981 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     8041 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     8101 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     8161 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     8221 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     8281 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8341 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8401 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8461 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8521 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8581 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8641 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8701 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8761 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8821 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8881 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8941 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     9001 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     9061 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     9121 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//