LOCUS       RR217620                9159 bp    DNA     circular  SYN 
DEFINITION  Cux2 (myc-DDK-tagged) - Rat cut-like homeobox 2 (Cux2)
ACCESSION   NM_001271380
VERSION     NM_001271380.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5316..5407 
                     /label="Myc-DDK"  
     misc_feature    5437..6022 
                     /label="PolyA signal"  
     misc_feature    5494..5513 
                     /label="Restriction Enzyme"  
     misc_feature    6298..6970 
                     /label="ColE1"  
     misc_feature    7400..8194 
                     /label="Kan/Neo"  
     misc_feature    8229..8531 
                     /label="SV40 ori"  
     misc_feature    8617..9094 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5298..5304 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5298 
                     /label="Cux2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTAGCCCCA GTGCTGAAAA GCTTCCAAGC TGAGGTGGTG GCTCTCAGTA 
     1081 AGAGAAGTCA GGAGGCAGAG GCAGCATTCC TGAGTGTTTA TAAGCAATTA ATTGAAGCGC 
     1141 CAGACCCTGT CCCGTCGGTT GAGGTGGCGC GGACTCTAGA CGACAGACTG CAGCGTCCCA 
     1201 GCTTCCACCC CAGTGGGCAG CGCCTACGAG ACGTGCACAT CTCGTGGAAG AGGTGCCCAG 
     1261 AGCCACCCAG TGCCAGAGAG CAGAACGAGG GGACGTGTCC CACCGGGCAC ACGCCAGCCA 
     1321 ACGGTAACCA CCTGCCAGGT CCCGAGGACA CCCTCGTGAC AGACACCTTG CTGCAGAAGG 
     1381 ATGAGGCCGA GAGACAGAAG GGCCTCCAAG AAGTCCACGT CACCTTGGCA GCCAGGCTGG 
     1441 GGGAGGCAGA GGAGAAAATC AAGGTGTTAC ATTCAGCGCT AAAGGCCACA CAGACGGAGC 
     1501 TGCTGGAGCT GAGGAGGAAA TACGATGAGG AGGCTGCATC CAAGGCCGAT GAGGTCGGCT 
     1561 TGATCATGAC GAACCTGGAG AAGGCCAACC AGCGAGCAGA GGCTGCCCAG CGGGAGGTAG 
     1621 AAAGCCTCCG GGAGCAGCTG GCATCGGTCA ACAGCTCCAT TCGCCTGGCT TGCTGCTCCC 
     1681 CGCAGGGACC CGGTGGGGAG AAGGTGAGCT TCGCCCTGTG CTCAGGGCCC CGGCTGGAGG 
     1741 CAGCGCTGGC TTCCAAGGAC AGGGAGATCC TCAGGCTGCT GAAGGACGCC CAGCAGCTGC 
     1801 GGCACTCTCT GCAGGAGCTG GAAGAGGTCT CAGCCAACCA AATCGCTGAT CTGGAGCGGC 
     1861 AGCTAGCGGC CAAGTCTGAG GCCATAGAGA AACTCCAAGA AAAGCTGGAG GCCCAGGCTG 
     1921 ACTATGAAGA GATTAAAACA GAGCTGAGCA TCCTGAGAGC CATGAAGCTG GCCTCCAGCA 
     1981 CGTGCAGCCT CCCACAGGCG CTGGGCAAGC CTGACGACCC ACTGCTCATG GCCAAGGATG 
     2041 CCTTCTTCCC CACACAGAAG CTCCTGCTGG AAAAGCCCGC GCTGCTGGCC AGCCCTGAGG 
     2101 AGGACCCCTC GGAGGATGAC TCTATCAAGG GCTCACTGGG CATGGAGCCC TCCTACCCTC 
     2161 CTCAGCTTCC ACCTCCGCCA GGCCCTGAAG ACCCGCTGTC CCCAAGCCCT ACGCAGCCCC 
     2221 TGCTGGGCCC CAGCCTGGGT CCTGATGGAC CAAGGACTTT CTCGCTGTCC CCCTTCCCCA 
     2281 GCCTGGCACC GGGGGAGAGG CTGGCTGGGG ACCCACTGCT GTCCAAACAT ATGATGGCCC 
     2341 CAGTTGCCTT CAAAGGGGAA ACGGGCAACC TGCTGGCATT CCCACCGGCC TTCTATGGCG 
     2401 GTGCCAAGCC TCCAACGGCT CCTGCCGCCC CAGTGCCCGG CCCTGAGCCC TCTGGGGCCC 
     2461 CAGAGCCTGT GGACGGGGCT GGGCCAGAGG AGGAGCAGCT GGACACGGCC GAGATCGCCT 
     2521 TCCAGGTGAA GGAGCAGCTT CTGAAGCACA ACATTGGCCA GCGTGTGTTT GGCCACTATG 
     2581 TGCTGGGACT GTCGCAGGGC TCGGTGAGTG AGATCCTGGC ACGGCCCAAG CCGTGGCGCA 
     2641 AGCTCACGGT GAAGGGCAAG GAGCCCTTCA TCAAGATGAA GCAGTTCCTG TCGGATGAGC 
     2701 AGAACGTGCT GGCCCTGCGC ACCATCCAGG TGAGGCAGCG AGGCAGCATC ACCCCGAGAA 
     2761 TCCGAACTCC TGAGACCGGC TCAGACGACG CCATCAAGAG TATCCTGGAG CAGGCCAAGA 
     2821 AGGAGATTGA GTCTCAGAAA GGGGGTGAGT CCAAGAACTC CCCTGCCTCC GTGAGCATCC 
     2881 CCAACGGCAC AGCTTCCTCC AGCACCTCGG AAGACGCCAT CAAGAACATT CTGGAACAAG 
     2941 CCCGCCGCGA AATGCAAGCC CAGCAGCAGG CCCTGCTGGA GATGGAGTCG GGTCCCAGGG 
     3001 GCCGCTCAGT GCCTCCCTCT CCTCCGGAGC GGCCCTCGCC AGCCACTATG AGCCAGAATG 
     3061 GGGCCCTGGC CTGCGTGAAG CAGGAAGACG GCGGCTGCAG CAGCAGCAGT AGCGGCAGTG 
     3121 CGCAGGCGCC GCTTGCTGTC CTGTCCCCCG CTGCATTTGT GCAGCGGATC ATCCGCAAGG 
     3181 TGAAGTCAGA GATCGGCGAC GCTGGCTACT TTGATCACCA CTGGGCATCA GACCGTGGTC 
     3241 TGCTCAGCCG TCCCTACGCC TCCGTGTCAC CTTCCCTCTC CTCCTCCTCC AGCTACTCGG 
     3301 GACAGCCCAA TGGGCGAGCC TGGCCCCGTG GGGACGAGGC AACCATTGCC CCTGAGGACG 
     3361 AAGCGGCTAC GGGCATGGGG GAGGACGAGG CCCCCAGGGT GGGAGAGCTC AAGGCCGAGG 
     3421 CCGGAGTCTC CGAGGTGGGT GGCGGGCGAC TGCCCTACTA CCCAGCGTAT GTGCCCCGCA 
     3481 CACTCAAGCC CACTGTGCCG CCCCTAACAC CCGAGCAATA CGAGCTATAC ATGTACCGGG 
     3541 AGGTAGACAC TCTGGAGCTC ACCCGCCAGG TCAAGGAGAA GCTAGCCAAG AACGGCATCT 
     3601 GCCAGCGCAT CTTTGGGGAG AAGGTCCTGG GACTGTCTCA GGGCAGCGTG AGTGACATGC 
     3661 TGTCACGGCC AAAGCCATGG AGCAAGCTGA CACAGAAGGG CCGGGAGCCT TTCATCCGCA 
     3721 TGCAGTTGTG GCTATCAGAC CAGCTGGGCC AGGGCCAGGC CCCAAACCAG CAGCCCAGCG 
     3781 CTAGCCAGGC CAGCCCCACG GAGCCAACGT CCTCACCATC ACCTCCCCCA AGCCCCACGG 
     3841 AGCCTGAAAA GACTTCCCAG GAGCCTCTGG GCCTGTCCCT AGAAAGCAGC AAGGAGAATC 
     3901 AGCAACCAGA GGGCCGGGCC AGCTCCACCC TGGGTGGGAA GCCCCTCTCA AGCAGCCAGA 
     3961 CTACGGGGGG CATCCAGGAG ATGGTGGCCA TGTCCCCAGA GCTGGACACA TACTCCATCA 
     4021 CCAAGAGAGT CAAGGAGGTC CTCACGGACA ACAACCTAGG GCAGCGGTTG TTTGGTGAGA 
     4081 GCATCCTGGG GCTAACACAG GGCTCCGTGT CAGACCTGCT GTCGAGGCCC AAGCCCTGGC 
     4141 ACAAGCTGAG CTTGAAGGGC CGGGAGCCCT TTGTGCGCAT GCGGCTGTGG CTGAGCGACC 
     4201 CCCACAATGT GGAAAAGCTT CGAGATGTGA AGAAGCTGGA GAAGAAAGCC TATCTGAAAC 
     4261 GCCGATACGG GCTCATCAGC ACGGGCTCTG ACAGCGAGTC ACCGGCAGCT CACTCCGAGT 
     4321 GTCCCAGCCC ATGTCTGCAG CCGCAGGAGT TGAGCCTCAT GCAGGCCAAG AAGCCACGTG 
     4381 TGGTGCTGGC ACCGGCCGAG AAGGAGGCTC TGCGGAAGGC CTACCAGCTA GAGCCCTACC 
     4441 CCTCACAGCA GACCATAGAG TTGCTCTCTT TCCAGCTCAA CCTCAAGACA AACACTGTCG 
     4501 TCAACTGGTT CCACAACTAC AGGTCCAGGA TGCGCCGGGA GATGCTGGTG GAGGGAACAC 
     4561 AGGATGACCC AGACTTTGAC CCAAGTGGGG GCTCCAGTGT CCTGCCATCA GGCCACACCC 
     4621 ACACAGACCC CACCCCGCAG AGCCCTGACT CAGAGATTGA GGACCAAAAG CCCCCCATGA 
     4681 AGAGCCTGGA GCTGCAGGAA CCTGAGAGTC CCGTACACCA AGCAGCCCCA GACAGGGCTG 
     4741 TGGTGAAGAT TAAGCAGGAA GAGGGTTTAG AGGTGGATGG GGACAGCCAG CCCCAGGACA 
     4801 TAGGGGATCC AGACAGAGGG CAAGGTGGCC CCAAAGAGGA GGATACTCAC CCTCTGGGGA 
     4861 ACAATGAACT CTCAGAGCTG GTCCCCGGGC CTTTCCTTCC AGGCACACCC AATCCAGACT 
     4921 GCTCTTCATT GCACACCCCC CAAGCAAAAG AGACTGGGGA ACAGGTTCAC TCAGAGCCTC 
     4981 TGAGTTTCAA GTCCACCTCT GAATCCTCAT GCTGCAGCCT GGAGGGGCCA CCGAACTCTC 
     5041 CCTCTGCCAT CTCCTCGCCA GACCTCATGA CATGTGTGTC ACCCGCCCCT TCCTCCTCAG 
     5101 CCCCCATCTC CCCATCCCTA CCTGGCACCC TACCTGCCAA AGTGCCGAGT ACCAGTCCCA 
     5161 CTGCTGACCC AGCTGCAGCC TTGCACCCCA GCACTAAGGT GAACCCCAAC TTGCAGCGGC 
     5221 GGCATGAGAA AATGGCCAAC TTGAACAGTA TAATCTACCG ACTGGAGAGG GCTGCCAACC 
     5281 GGGAAGAGGC TCTGGAGTGG GAATTCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     5341 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5401 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5461 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5521 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5581 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5641 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5701 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5761 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5821 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5881 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5941 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     6001 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     6061 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     6121 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     6181 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     6241 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6301 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6361 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6421 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6481 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6541 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6601 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6661 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6721 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6781 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6841 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6901 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6961 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     7021 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     7081 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     7141 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     7201 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     7261 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     7321 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7381 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7441 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7501 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7561 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7621 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7681 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7741 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7801 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7861 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7921 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7981 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     8041 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     8101 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     8161 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     8221 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     8281 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     8341 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8401 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8461 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8521 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8581 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8641 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8701 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8761 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8821 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8881 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8941 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     9001 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     9061 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     9121 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//