LOCUS       RR217609                9033 bp    DNA     circular  SYN 
DEFINITION  Map3k5 (myc-DDK-tagged) - Rat mitogen-activated protein kinase kinase kinase 5 (Map3k5)
ACCESSION   NM_001277694
VERSION     NM_001277694.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5188..5279 
                     /label="Myc-DDK"  
     misc_feature    5309..5894 
                     /label="PolyA signal"  
     misc_feature    5366..5385 
                     /label="Restriction Enzyme"  
     misc_feature    6170..6842 
                     /label="ColE1"  
     misc_feature    7272..8066 
                     /label="Kan/Neo"  
     misc_feature    8101..8403 
                     /label="SV40 ori"  
     misc_feature    8489..8966 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5163..5170 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5163 
                     /label="Map3k5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCACCGAA GCCGGCGAGG GCATCACTTT CTCCGTGCCG CCCATTTTGT 
     1081 CCGCGGGTTT CTGCCCCATC CCGGAGGGCG GCAGCTGCCG GAGGGGAGGA GGAGCGGCGA 
     1141 CGGCGGCGGA GGGCGAGCCG AGCCTGCAGC CCTTGCTTAT GCCGCCGCCG CCACCACCGC 
     1201 CGGGCAGTTT CTGGAACGTG GAGAGCGCGG CGGCTCCTGG CACCAGCTGC CCTACCACCG 
     1261 CCCCCGGGAG CAGCGCCACC CGAGGCCGGG GCAACTCTGG CAGCGGGGGC GGCCGGCGTA 
     1321 CCACCGTGGC GTATGTGATC AACGAGGCGA GCCAGGGGCA GCTGGTGGTG GCGGAGAGCG 
     1381 AGGCCCTGCA GAGCCTGCGG GAGGCGTGCG AGGCGGTGGG CGCCACCCTG GAGACCCTGC 
     1441 ATTTCGGGAA GCTGGACTTT GGGGAAACCA CCGTGCTGGA CCGTTTTTAC AACGCTGACA 
     1501 TCGCCGTGGT AGAGATGAGT GACGCCTTCC GGCAGCCTTC CCTGTTTTAT CATCTCGGAG 
     1561 TGAGAGAGAG CTTCAGCATG GCCAACAACA TCATCCTCTA CTGCGATACT AATTCAGACT 
     1621 CGCTTCAGTC CCTGAAGGAA ATCATTTGCC AGAAGAACAC AGTGTGCACT GGGAACTACA 
     1681 CCTTCATCCC CTACATGGTG ACACCACACA ACAAGGTCTA CTGCTGTGAC AGCAGCTTCA 
     1741 TGAAGGGCCT GACCGAGCTC ATGCAGCCCA ACTTCGAGCT CCTGCTGGGG CCGATCTGCT 
     1801 TACCTCTCGT GGACCGTTTC GTTCAGCTCC TGAAGGTGGC GCAAGCCAGT TCCAGCCAGT 
     1861 ACTTCCGGGA ATCTATACTC AACGACATCA GGAAAGCTCG GAATCTGTAC ACTGGTAAAG 
     1921 AGTTGGCGGC TGAGCTGGCG AGGATCCGGC AGCGGGTAGA CAATATTGAA GTTCTGACGG 
     1981 CAGACATTGT TATCAACCTG TTGCTTTCCT ACAGAGATAT CCAGGACTAT GACTCCATAG 
     2041 TGAAGCTGGT AGAGACCTTA GAAAAACTAC CGACCTTCGA TCTGGCCTCG CACCACCACG 
     2101 TGAAGTTTCA TTATGCCTTT GCACTGAACA GGAGAAATCT CCCTGGAGAC AGAGCAAAAG 
     2161 CTCTTGACAT CATGATCCCC ATGGTGCAAA GTGAAGAGCA AGTTGCTTCG GATATGTACT 
     2221 GTCTCGTTGG CCGAATCTAC AAAGACATGT TTTTGGATTC AAATTTCACT GACACCGAAA 
     2281 GCAGGGACCA TGGAGCATCT TGGTTCAAAA AGGCGTTTGA GTCTGAGCCA ACACTACAGT 
     2341 CAGGAATTAA TTACGCAGTC CTCTTGCTGG CGGCTGGGCA CCAGTTTGAA TCTTCTTTTG 
     2401 AGCTTCGGAA AGTTGGAGTT AAACTGAGCA GCCTTCTGGG TAAAAAGGGG AACTTGGAGA 
     2461 AACTCCAGAG CTACTGGGAA GTCGGATTTT TTCTGGGAGC CAGCGTCCTG GCCAACGACC 
     2521 ATCTGAGGGT CATTCAGGCA TCTGAGAAAC TGTTCAGACT GAAGACGCCA GCGTGGTACC 
     2581 TCAAGTCTAT TGTGGAGACC ATTTTGATAT ATAAGCATTT TGTGAAACTG ACCACAGAGC 
     2641 AGCCCACAGC CAAGCAGGAG CTCGTGGACT TCTGGATGGA TTTCCTGGTG GAGGCCACAA 
     2701 AGACAGATGT TACTGTCGTT AGATTTCCAG TGTTAATATT AGAGCCAACG AAAATCTATC 
     2761 AGCCTTCTTA CCTGTCGATC AACAATGAAG TCGAGGAGAA GACGATCTCT ATTTGGCATG 
     2821 TCCTCCCTGA CGACAAGAAG GGTATCCATG AGTGGAACTT TAACGCTTCC TCTGTCAGGG 
     2881 GGGTGAGTAT CTCCAAGTTT GAAGAACGAT GCTGTTTCCT TTATGTACTT CACAATTCCG 
     2941 ACGACTTCCA AATCTACTTC TGCACAGAAC TGCACTGTAA AAGGTTTTTC GAAATGGTGA 
     3001 ACACCATCAC CGAAGAGAAG GGGAGAGGCG CGGAGGAAGG AGACTGTGAA GGTGACTCCC 
     3061 TAGAGTATGA CTACGAACAC GATGAAAATG GAGACAGAGT TGTGCTAGGA AAGGGGACTT 
     3121 ACGGGATAGT CTACGCAGGC CGAGACCTGA GCAACCAGGT CAGAATTGCT ATTAAGGAAA 
     3181 TCCCAGAGAG AGACAGCAGA TACTCTCAGC CCCTGCATGA GGAAATTGCC TTACACAAGC 
     3241 ATCTGAAGCA CAAGAACATT GTCCAGTATC TGGGCTCCTT CAGTGAGAAC GGCTTCATCA 
     3301 AAATCTTCAT GGAGCAAGTC CCAGGAGGAA GTCTCTCTGC TCTCCTCCGT TCAAAATGGG 
     3361 GCCCACTAAA AGACAATGAG CAGACGATTG GCTTCTATAC GAAGCAAATA TTGGAAGGAT 
     3421 TAAAATATCT TCATGACAAT CAGATAGTCC ACCGGGATAT AAAGGGTGAC AATGTATTGA 
     3481 TTAATACCTA CAGTGGCGTG CTCAAGATCT CTGACTTTGG GACATCCAAG AGGCTTGCTG 
     3541 GCATAAACCC GTGCACCGAA ACCTTTACAG GTACCCTTCA GTATATGGCG CCAGAAATCA 
     3601 TCGATAAAGG ACCACGAGGC TATGGAAAGG CCGCCGACAT TTGGTCACTG GGCTGTACGA 
     3661 TCATCGAAAT GGCTACTGGA AAACCACCGT TTTATGAACT AGGAGAGCCC CAGGCAGCCA 
     3721 TGTTCAAGGT GGGGATGTTT AAGGTCCACC CCGAGATCCC AGAGTCCATG TCTGCCGAGG 
     3781 CCAAGGCGTT CATACTGAAG TGTTTTGAAC CAGACCCTGA CAAGAGAGCC TGTGCTAATG 
     3841 ACCTGCTTGT TGATGAGTTC CTAAAAGTTT CCAGCAAAAA GAAGAAGACA CAGCCCAAAC 
     3901 TCTCGGGTAT GTGGTTGCAC GGAGAGGAGT ATCTTAGAAG CATCTCGCTG CCGGTCCCTG 
     3961 TCCTGGTAGA AGACACCAGC AGCAGCAGCG AGTATGGCTC CGTTTCTCCG GACACAGAGC 
     4021 TGAAGGCCGA CCCCTTCTCC TTCAAAACCA GAGCTAAGTC CTGTGGAGAA AAGGACGGGA 
     4081 AAGGGATCCG GACGCTGTTC CTGGGCATTC CGGATGAAAA CTTTGAAGAT CACAGTGCGC 
     4141 CCCCGTCCCC TGAAGAGAAA GACTCCGGCT TTTTTATGCT GAGAAAGGAT AGCGAAAGGC 
     4201 GGGCGACCCT TCACAGGATC CTGACAGAAG ACCAGGACAA GGTTGTGAGG AACCTAATGG 
     4261 AGTCTCTGGC CCAGGGTCCT GAAGAGCCTA AACTCAAGTG GGAACACATC ACAACTCTCA 
     4321 TCTCAAGCCT CAGAGAGTTT GTGAGGTCCA CTGACCGGAA AATCATAGCC ACTACACTGT 
     4381 CCAGGCTCAA GCTGGAGCTG GACTTCGACA GCCATGGCAT CAGCCAGGTG CAGGTGGTCC 
     4441 TCTTTGGCTT CCAGGATGCG GTCAATAAAG TTCTCCGGAA TCATAACATC AAGCCACACT 
     4501 GGATGTTTGC CTTGGACAGT ATCATTCGGA AGGCTGTGCA GACAGCTATC ACCATCCTGG 
     4561 TTCCAGAGTT GAGGCCACAT TTCAGCCTCG CGTCGGAGAG CGACACGGCG GATCCGGAAG 
     4621 ACTTGGATGT CGACGATGAA CACGAGGAAT CGCCTTCGAA TCAAACCGCG CGGAGGCCCC 
     4681 AACCCGTCGC GGAGGATGCC GTGGCTACCT CAGGGGTGAG CACGCTCAGC TCCACCGTAT 
     4741 CCCATGATTC CCAGAATGCA CACCGCTCGC TGAATGTGCA GCTTGGGAGG ATGAAGATGG 
     4801 AAACTAACAG ATTACTTGAA GAGTTGGTTC GGAAAGAGAA AGAGTTGCAG GCACTCCTTC 
     4861 ATCAAGCCAT TGAAGAAAAG GACCAAGAAA TTAAGCACCT GAGACTTAAG TCCCAACCCA 
     4921 TAGATACCCC TGGGTTTCCC GTGTGCCACC TGAACGCTCC TGGTACAGCC ATTGAAGACT 
     4981 CTGAACTTAC TGGCTGGCTG AGGGAAAATG GAGCTGACGA AGACACTATA AGTCGGTTTC 
     5041 TGGCTGAGGA TTACACGCTG GTGGATGTCC TTTACTACGT CACGCGTGAC GACTTGAAGT 
     5101 GCCTCCGACT AAGGGGAGGG ATGCTGTGCA CGCTGTGGAA GGCCATCATT GACTTTCGGA 
     5161 ACAAAGGGGC CAGCGGACCG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5221 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5281 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5341 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5401 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5461 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5521 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5581 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5641 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5701 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5761 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5821 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5881 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5941 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6001 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6061 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6121 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6181 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6241 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6301 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6361 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6421 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6481 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6541 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6601 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6661 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6721 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6781 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6841 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6901 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6961 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7021 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7081 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7141 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7201 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7261 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7321 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7381 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7441 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7501 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7561 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7621 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7681 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7741 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7801 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7861 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7921 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7981 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8041 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8101 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8161 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8221 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8281 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8341 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8401 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8461 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8521 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8581 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8641 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8701 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8761 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8821 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8881 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8941 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9001 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//