LOCUS       RR217604                9000 bp    DNA     circular  SYN 
DEFINITION  Drosha (myc-DDK-tagged) - Rat drosha, ribonuclease type III (Drosha)
ACCESSION   NM_001107655
VERSION     NM_001107655.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5157..5248 
                     /label="Myc-DDK"  
     misc_feature    5278..5863 
                     /label="PolyA signal"  
     misc_feature    5335..5354 
                     /label="Restriction Enzyme"  
     misc_feature    6139..6811 
                     /label="ColE1"  
     misc_feature    7241..8035 
                     /label="Kan/Neo"  
     misc_feature    8070..8372 
                     /label="SV40 ori"  
     misc_feature    8458..8935 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5139..5145 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5139 
                     /label="Drosha"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAAGGCAAT ACGTGTCATA GAATGTCTTA CCACCCAGGA CGAGGGTGTC 
     1081 CCCGGGGACG AGGAGGACAT GGAGCCAGAC CCTCAGCACC GACTTACAGG CCCCAAAATC 
     1141 TGAGACTTCT TCACCCTCAG CAGCCTCCTG CGCAGTATCA ATATGAGCCT CCCAGTGCCC 
     1201 CCTCTTCCTC GTTCTCGAAC TCTCAAGCCC CCAACTTTAT GCCCCCACGG CCTGACTTTG 
     1261 TCCCTTACCC TCCACCAGTT GCACCGTCAG CACAGGGCCC TCTCCCACCC TGCCCAGTTA 
     1321 GGCCCCCTTA CCCCAACCAC CAGATGAGAC ACCCTTTCCC TGTGCCTCCC TGTTTTCCAC 
     1381 CCATGCCCCC TCCAATGCCT TGTCCCAACA ACCCCCCAGT CTCCGGAGCA CCTCCCGGCC 
     1441 AAGGCACTTT CCCCTTCATG GTCCCCCCTC CTTCCATGCC CCACCCCCCA CCCCCTCCCG 
     1501 TCATGCCGCA GCAGGTTAAT TATCAGTACC CTCCTGGATA CTCGCACAGT TTCCCGCCTC 
     1561 CCAATTTCAA CAGTTTTCAG AACAACTCCA GTTCTTTCCC ACCCAGTGCT AATAACAGCA 
     1621 GCACCCCTCA TTTTAGACAC CTCCCTCCAT ACTCACTTCC CAAGGCTCAG AGTGAGAGAA 
     1681 GGTCCCCAGA GAGGCTTAAG CACTACGATG ACCACAGGCA CCGAGATCAC AGTCACGGGC 
     1741 GAGGTGAGAG GCATCGGTCC CTGGAGCGCA GAGAGCGCGG CCGCAGCCCG GAGAGGAGAA 
     1801 GACCTGAGAG CCGCTACCGC TCGGAATATG ATCGGGGGAG GACGCCACCG CCGCGCCATC 
     1861 GCAGCTACGA AAGGAGCAGA GAGCGGGAAC GAGAGAGACA CAGGCACCGG GAAAACCGCA 
     1921 GATCACCGTC TCTGGAAAGG GCCTACAAAA AGGAATATAA GAGATCTGGA AGGAGCTATG 
     1981 GCTTACCAGT CACTCCTGAG CCGGCTGGAT GCACAGCAGA ATTACCTGGG GAGATTATTA 
     2041 AAAATACAGA TTCTTGGGCC CCACCCCAGG AGAATGTGAA TCATCGTTCT CCAAGCAGGG 
     2101 AGAAGAAGAG AGCACGTTGG GAGGAGGAAA AAGACAGGTG GAGTGACAGC CAGAGCTCTG 
     2161 GCAAAGAGAA GAGCTATACG TCCATCAAAG AAAAGGAACC AGAGGAGGCG CCGCCTGAGA 
     2221 AAACCGAGGA AGAGGACGAA GAGCTCCTCA AGCCCGTGTG GATCCGATGC ACACACTCGG 
     2281 AAAGTTACTA CTCCAGTGAC CCCATGGATC AGGTGGGAGA CTCTACTGTC GTAGGAACAA 
     2341 GCAGGCTCCG GGATCTGTAT GACAAATTTG AGGAAGAGTT GGGGAACAGG CAAGAGAAGG 
     2401 CCAAAGCTGC CCGGCCTCCA TGGGAGCCCC CAAAGACAAA GCTGGATGAA GATTTAGAGA 
     2461 GCTCCAGTGA GTCTGAATGC GAGACGGACG AGGACAGCAC CTGCTCAAGC AGCTCGGACT 
     2521 CGGAGGTGTT CGATGTCATT GCAGAGATTA AACGCAAAAA GGCTCACCCT GACCGACTTC 
     2581 ATGATGAACT TTGGTACAAC GACCCAGGCC AGATGAACGA CGGACCACTT TGCAAATGCA 
     2641 GTGCAAAAGC AAGACGCACA GGAATTCGGC ACAGCATTTA TCCCGGAGAA GAGGCAATCA 
     2701 AGCCCTGTCG TCCTATGACC AACAACGCTG GACGACTTTT CCACTACCGG ATTACCGTCT 
     2761 CCCCGCCTAC CAACTTTTTA ACGGACAGGC CAACAGTCAT AGAATACGAT GATCATGAGT 
     2821 ATATCTTTGA AGGATTTTCT ATGTTTGCAC ATGCCCCTCT GACCAATATT CCACTGTGTA 
     2881 AAGTAATTCG ATTCAACATA GACTACACGA TTCATTTTAT TGAAGAGATG ATGCCTGAGA 
     2941 ATTTTTGTGT GAAAGGACTT GAACTGTTTT CATTGTTCCT ATTCAGAGAT ATTTTGGAGT 
     3001 TATACGACTG GAATCTTAAA GGTCCTTTGT TTGAAGACAG CCCTCCCTGC TGTCCAAGAT 
     3061 TTCATTTCAT GCCACGTTTT GTAAGATTTC TTCCAGATGG CGGCAAAGAA GTGTTATCAA 
     3121 TGCACCAGAT TCTTCTCTAC CTGCTTCGCT GTAGCAAGGC TTTGGTCCCT GAGGAGGAGA 
     3181 TTGCCAATAT GCTCCAATGG GAGGAGCTCG AGTGGCAGAA ATATGCGGAA GAGTGCAAAG 
     3241 GCATGATAGT TACCAACCCT GGGACGAAAC CAAGCTCTGT CCGCATCGAT CAACTGGATC 
     3301 GTGAGCAGTT CAACCCTGAA GTGATCACTT TTCCGATTAT CGTTCACTTT GGGATACGCC 
     3361 CTGCACAGTT GAGTTATGCA GGAGACCCAC AATACCAAAA ATTATGGAAA AGTTACGTGA 
     3421 AACTTCGCCA CCTCTTAGCA AATAGTCCCA AGGTCAAACA GACTGACAAA CAGAAGCTGG 
     3481 CGCAGAGGGA GGAAGCGCTC CAGAAGATAC GGCAGAAGAA CACAATGCGT CGAGAAGTCA 
     3541 CCGTGGAGCT GAGTAGCCAA GGATTCTGGA AAACTGGCAT TCGTTCTGAT GTCTGTCAGC 
     3601 ACGCGATGAT GCTGCCTGTT CTGACCCATC ACATCCGATA CCATCAATGC TTAATGCACT 
     3661 TGGACAAGTT GATAGGGTAT ACTTTTCAGG ATCGTTGTCT GCTCCAGCTG GCCATGACTC 
     3721 ATCCGAGTCA TCATTTAAAT TTTGGAATGA ATCCTGATCA TGCCAGGAAT TCTCTGTCTA 
     3781 ACTGTGGAAT TCGACAGCCC AAATATGGAG ACAGAAAAGT TCATCACATG CACATGCGGA 
     3841 AAAAAGGAAT TAACACCCTA ATAAATATCA TGTCACGCCT CGGCCAAGAT GATCCAACTC 
     3901 CTTCAAGGAT TAATCACAAT GAAAGGTTGG AGTTCCTGGG AGATGCTGTT GTTGAGTTTC 
     3961 TGACCAGTGT CCACCTGTAC TATTTGTTTC CTAGCTTGGA GGAAGGAGGC TTGGCAACCT 
     4021 ATCGGACAGC CATTGTTCAG AATCAGCACC TCGCCATGCT CGCAAAGAAA CTTGAACTGG 
     4081 ATCGATTTAT GCTGTATGCC CATGGGCCCG ACCTATGTAG AGAATCAGAC CTCCGGCATG 
     4141 CGATGGCCAA TTGTTTCGAA GCATTGATAG GAGCTGTTTA CTTGGAGGGG ACCCTGGAGG 
     4201 AAGCCAAACA GTTGTTTGGA CGCTTACTCT TTAATGATCC GGACCTGAGA GAAGTCTGGC 
     4261 TCAATTATCC TCTCCACCCA CTCCAACTAC AAGAGCCAAA TACAGATCGA CAACTTATTG 
     4321 AAACTTCCCC AGTTCTACAG AAACTGACCG AGTTTGAAGA AGCAATTGGG GTGATCTTCA 
     4381 CTCACGTCCG GCTTCTGGCA CGGGCATTCA CACTAAGAAC TGTGGGCTTT AACCACCTGA 
     4441 CCCTAGGCCA CAATCAGAGG ATGGAATTCC TGGGTGACTC CATAATGCAG CTGGTAGCCA 
     4501 CGGAGTACTT GTTCATCCAC TTCCCCGACC ACCACGAAGG ACACTTGACG TTGTTACGAA 
     4561 GTTCTTTAGT GAACAACAGA ACTCAAGCCA AGGTAGCAGA GGAACTGGGC ATGCAAGAGT 
     4621 ATGCCATCAC CAACGACAAA ACCAAGAGAC CCGTGGCCCT AAGAACCAAG ACTTTGGCAG 
     4681 ACCTTTTGGA ATCATTTATT GCAGCCCTGT ACATCGACAA GGACTTGGAA TACGTCCATA 
     4741 CTTTCATGAA CGTCTGCTTC TTTCCACGTC TGAAGGAGTT CATTCTGAAC CAGGACTGGA 
     4801 ATGACCCCAA GTCGCAGCTG CAGCAGTGCT GCCTGACCCT GAGGACTGAA GGGAAAGAAC 
     4861 CAGACATCCC CTTGTACAAG ACTCTGCAGA CAGTGGGGCC GTCCCACGCT AGAACCTACA 
     4921 CGGTGGCCGT TTACTTCAAA GGAGAGAGGA TAGGCTGTGG GAAAGGACCA AGTATTCAGC 
     4981 AGGCCGAAAT GGGAGCAGCA ATGGACGCAC TTGAGAAATA TAACTTTCCC CAGATGGCTC 
     5041 ATCAGAAGCG GTTCATTGAA CGGAAATACA GACAAGAGTT AAAGGAAATG AGGTGGGAAA 
     5101 GAGAGCATCA GGAGAGAGAG CCGGATGAGG CTGAAGACAT AAAGAAGACG CGTACGCGGC 
     5161 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5221 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5281 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5341 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5401 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5461 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5521 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5581 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5641 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5701 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5761 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5821 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5881 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5941 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     6001 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     6061 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     6121 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6181 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6241 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6301 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6361 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6421 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6481 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6541 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6601 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6661 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6721 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6781 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6841 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6901 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6961 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     7021 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     7081 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     7141 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7201 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7261 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7321 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7381 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7441 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7501 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7561 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7621 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7681 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7741 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7801 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7861 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7921 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7981 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     8041 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     8101 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     8161 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8221 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8281 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8341 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8401 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8461 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8521 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8581 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8641 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8701 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8761 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8821 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8881 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8941 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//