LOCUS       RR217603                8991 bp    DNA     circular  SYN 
DEFINITION  Aatk (myc-DDK-tagged) - Rat apoptosis-associated tyrosine kinase (Aatk)
ACCESSION   NM_001168703
VERSION     NM_001168703.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5148..5239 
                     /label="Myc-DDK"  
     misc_feature    5269..5854 
                     /label="PolyA signal"  
     misc_feature    5326..5345 
                     /label="Restriction Enzyme"  
     misc_feature    6130..6802 
                     /label="ColE1"  
     misc_feature    7232..8026 
                     /label="Kan/Neo"  
     misc_feature    8061..8363 
                     /label="SV40 ori"  
     misc_feature    8449..8926 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5130..5136 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5130 
                     /label="Aatk"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCATCGCG CTGCTGGCCC TGGCCATGTC GTCGTCCTTC TTCAACCCCA 
     1081 GCTTTGCCTT CAGCTCCCAC TTCGACCCGG ACGGTGCCCC GCTCAGTGAG CTATCCTGGT 
     1141 CCTCGTCCCT TGCAGTGGTT GCTGTGTCCT TCTCTGGGAT CTTCACTGTC ATTGTCCTCA 
     1201 TGCTGGCCTG CCTGTGCTGT AAGAAGGGCG GCATTGGGTT CAAGGAGTTT GAGAATGCTG 
     1261 AAGGGGAAGA GTATGTGGCC GACTTCTCGG AGCAGGGCTC CCCGGCTGCT ACTGTACAGA 
     1321 ACGGCCCCGA TGTGTATGTC CTGCCTCTCA CTGAGGTCTC CCTGCCCATG GCTAAGCAGC 
     1381 CGGGTCGCTC AGTGCAACTC CTCAAGTCCA CGGACCTGGG CCGCCACAGC CTCCTGTACT 
     1441 TGAAGGAGAT TGGCCACGGT TGGTTTGGGA AGGTGTTCTT GGGGGAGGTG CACTCGGGTG 
     1501 TCAGTGGCAC GCAGGTGGTG GTGAAGGAGC TGAAGGCCAG CGCCAGTGTG CAGGAGCAGA 
     1561 TGCAGTTCCT GGAGGAGGCG CAGCCCTACA GGGCCCTGCA GCACAGCAAC CTGCTTCAGT 
     1621 GCCTGGCCCA GTGTGCTGAG GTGACCCCCT ATTTGCTGGT TATGGAGTTC TGTCCCCTGG 
     1681 GGGACCTCAA AGGTTATCTA CGGAGCTGCC GGGTGACAGA GTCCATGGCG CCTGACCCCC 
     1741 TTACCTTGCA GCGCATGGCG TGCGAGGTGG CGTGTGGTGT CTTGCATCTA CATCGTCACA 
     1801 ACTATGTGCA CAGTGACCTG GCCCTGAGGA ACTGCCTGCT GACGGCTGAC CTGACAGTGA 
     1861 AGGTTGGCGA CTATGGCCTG GCCCATTGCA AATACAGGGA AGACTACCTC GTGACGGCCG 
     1921 ACCAGTTGTG GGTGCCGCTG CGTTGGATCG CGCCAGAGCT GGTGGATGAG GTGCATGGCA 
     1981 ACCTACTGGT GGTGGACCAG ACTAAGACCA GCAATGTGTG GTCCCTGGGT GTGACCATCT 
     2041 GGGAGCTTTT CGAGTTGGGC GCGCAGCCCT ACCCCCAGCA CTCGGACCGG CAGGTGCTGG 
     2101 CTTACGCCGT CCGAGAACAG CAACTTAAGT TGCCCAAGCC CCAGCTACAG CTGACTCTGT 
     2161 CTGATCGATG GTACGAGGTG ATGCAGTTCT GCTGGCTTCA GCCAGAACAG AGGCCTACGG 
     2221 CTGAGGAGGT TCATCTGCTG CTGTCCTACT TGTGCGCCAA GGGCACCACG GAGCTGGAGG 
     2281 AGGAGTTCGA GCGGCGCTGG CGCTCCCTGC GGCCGGGGGG CAGCGCGGGG CTGGGCTCAG 
     2341 CTTCCACAGT CCCGGCAGCT GCCGCATCTG AACTCACTGC TGCTTCATCT TTCCCGCTGC 
     2401 TGGAGCAGTT CACCAGTGAC GGCTTCCACG TGGACAGCGA CGACGTGCTG ACAGTCACTG 
     2461 AGACGAGCCA CGGCCTCAAC TTCGAATACA AGTGGGAGGC TGGCTGTGGC GCTGAGGCTT 
     2521 ACCCGCCCCC AGGCGCTGCT TTCAGCCCAG GTTCCGCAGG GCGCCTGCAG GAGCTGTGTG 
     2581 CACCCGACAG CTCGCCGCCG GGTGTGGTGC CGGTCCTCAG TGCCCACAGC CCCTCAGTGG 
     2641 GTAGCGAGTA CTTCATCCGC CTGGAGGGGG CAGTGCCGGC TGCTGGCCAT GACCCAGACT 
     2701 GTGCCGGCTG TGCTCCCAGC CCCCAAGCTG TGTCCGAGCA GGACAATAAC TCTGAGGAGA 
     2761 GCACCGCTGC ATCCCTTGTC ATGGAGCCGC TTCTGGGCCA CGCACCACCC ACTGGGGGCC 
     2821 TGTGGGGCCC CTGCGACCAT CATTCTCGAA GGAGGCAAGA GCCACCCTGC CCCTCACGCT 
     2881 CACCCTCTCC TGGGACCCCG ATGTTGCCAG CTGAAGACAT AGACTGGGGT GTAGCTACCT 
     2941 TCTGCCCACC CTTTTTTGAT GACCCACTGG GCACATCTCC CTCTGGGAGT CCTGGGGCCC 
     3001 AGCCATCCCC CAGTGATGAG GAGCTGGAGG AGGGAAAGAC TGGGAGGGCT GCTCAGTGTG 
     3061 GACACTGGAG CTCTAACATG TCTGCCAACA ATAACAGTGG CAGTCGAGAC CCAGAATCCT 
     3121 GGGATCCTGG CTATGTGAGC AGCTTCACAG ACAGCTACAG GGACGACTGC TCCAGCTTAG 
     3181 AGCAGACCCC TCGGGCCTCC CCTGAGCTGG GCCATCCCCT GTCCCAGGAG GATTCCAGAG 
     3241 ATTTTTTACC TGGGCTAGTA GCAGCTTCCC CTGGCCAGGA GTCAAGCCGT TGCTTCAACC 
     3301 TGCTCCCTCT GTGTCCTGCC AAAGGCCTGG CACCTGCTGC TTGCCTCGTC CCACCCCCCT 
     3361 GGACAGAGGC AGCTGTAGGT GGGGCTGAGA ACCCCATTGT CGAACCCAAA CTTGCCCAGG 
     3421 AGGCTGAGGG CTCTGCTGAA CCCCAGCTAC CCCTTCCTTC TGTCCCCTCC CCATCCCACG 
     3481 AAGGAGCCTC GCTTCCCTCG GAGGAGGCAA GCGCTCCTGA CATCCAGCCT GCCTCTCCTA 
     3541 CACCCGCTGC TGGCAGCTGG GTGACCGTCC CTGAGCCAGC CCCCACCCTG GATAGCAGCA 
     3601 GCAGTTCTCT GGGGCAAGAG GTACCCAGCA GCGAGGATGA AGACACAACC GAGGTTACGT 
     3661 CAGGAGTCTT CACCGACCTG TCCAGCGACG GCCCACACAC GGAGAAGTCA GGCATAGTAC 
     3721 CAGCCTTGCG CTGTCTGCAG AAGCAGGTGG GGACCCCTGA CTCCCTGGAC TCTTTGGACA 
     3781 TCCCGTCCTC AGCCAGCGAT GGCGGCTGTG AGGTCTTAAG CCCATCAGCT GCTGGCCCCG 
     3841 CTGGTGGGCA GCCCCGTGCC ATGGACAGTG GCTATGATAC AGAGAACTAT GAGTCCCCTG 
     3901 AGTTTGTGCT CAAGGAGGCC CATGAGTCAA GTGAGCCTGA GGCCTTTGGG GAGCCAGCCT 
     3961 CAGAGGGTGA GAGCCCAGGG CCCGATACTC TGCTCTCTGT CTCCCTTGGT GACCTCAGCA 
     4021 GAAAGAACCC CTACCGAGAC TCTGCCTACT TTTCAGACCT GGATGCTGAG TCCGAACCCA 
     4081 ACTTTGGCCC TGAGAAGCAC AGTGGGATCC AGGACTCCCA AAAGGAGCAA GACCTGAGGA 
     4141 GCCCACCTAG TCCAGGGCAT GAGTCTGTGC AGGCTTTTCC CAGGCCTGAG GTGTCCAGCG 
     4201 AGGTGCTGCC CCCTCCACAG CAGTCAGAGG AGCCCATGCC AGAAGGCCCC GGGTCAGAGC 
     4261 CTCTTGAGGC TCAAGGCCCA GTTGAAGTGG AGCCCGTGCC AGGCCCAAGT CATTCCAAAT 
     4321 GTCTCCTGTT GACCTCCGCT CCACTGAGCT CGGAAGGCAA TGGCACGGAG TCCCGGGGTC 
     4381 CCCCAGGACC ACTGTCAGGG CCAGCCCAGA CCGGGCGGAT GGGGAATCCT AGCACATCCA 
     4441 GATCCCCGCT CTGCCTGGCC CTGCCTGGCC ACCCTGGGAC TTCAGAGGGA CGGCCAGAGG 
     4501 ATGACGAGGA CACCGAGGAC AGTGAGGAGT CTGACGAGGA GCTCCGGTGC TACAGCGTCC 
     4561 AGGAGCCCAG TGAGGACAGT GAGGAGGAGC CATCAGCGGT GCCTGTGGTA GTGGCTGAGA 
     4621 GCCAGAGTGC CCGGAATCTA CGCAGCCTGC TGAAGATGCC CAGCCTGCTG TCGGAGGCCT 
     4681 TCTGCGACGA CCTGGAGCGC AAGAAGAAGG CTGTGTCCTT CTTTGATGAC GTCACGGTCT 
     4741 ACCTCTTTGA CCAGGAGAGC CCCACCCGAG AAGCTGGGGA GCCCTTCCCC AGCACGAAGG 
     4801 AGTGTCCCAC GTTCCTGGAG GGTAGCCCCG GCTCACCCAG TGCCACTGGC GTGCCACTGC 
     4861 GGGCTGGTCA CTCGCCAGAC AGCTCTGCTC CCGAACCGGG CAGTAGGTTC GAATGGGATG 
     4921 GAGATTTCCC ACTGGTGCCG GGCAAGGCTG CTTTGGTGAC TGCGCTGGAT CCTGCCGACC 
     4981 CTGTCCTGGC TGCGCCTCCC ACGCCAGCTG CACCATTTTC ACGCTTCACG GTGTCGCCCG 
     5041 CACCTCCCTC CCGCTTTTCC ATCACACAAG TATCCGACTC AGATGCCCAG TCCATGGGAG 
     5101 GCCCGGCAGC AGATGCTGAG GGCCGATGCA CAAAGGCTAC GCGTACGCGG CCGCTCGAGC 
     5161 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5221 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5281 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5341 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5401 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5461 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5521 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5581 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5641 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5701 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5761 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5821 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5881 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5941 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6001 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6061 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6121 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6181 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6241 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6301 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6361 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6421 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6481 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6541 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6601 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6661 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6721 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6781 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6841 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6901 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6961 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7021 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7081 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7141 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7201 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7261 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7321 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7381 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7441 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7501 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7561 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7621 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7681 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7741 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7801 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7861 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7921 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7981 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8041 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8101 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8161 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8221 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8281 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8341 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8401 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8461 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8521 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8581 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8641 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8701 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8761 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8821 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8881 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8941 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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