LOCUS       RR217602                8991 bp    DNA     circular  SYN 
DEFINITION  Med23 (myc-DDK-tagged) - Rat mediator complex subunit 23 (Med23)
ACCESSION   NM_001277054
VERSION     NM_001277054.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5148..5239 
                     /label="Myc-DDK"  
     misc_feature    5269..5854 
                     /label="PolyA signal"  
     misc_feature    5326..5345 
                     /label="Restriction Enzyme"  
     misc_feature    6130..6802 
                     /label="ColE1"  
     misc_feature    7232..8026 
                     /label="Kan/Neo"  
     misc_feature    8061..8363 
                     /label="SV40 ori"  
     misc_feature    8449..8926 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5130..5136 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5130 
                     /label="Med23"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTACCAATG GAGACGCAAC TGCAGAGCAT TTTCGAGGAG GTGGTGAAAA 
     1081 CAGAAATTAT AGAAGAGGCT TTCCCAGGCA TGTTTATGGA TACCCCCGAG GATGAAAAAA 
     1141 CAAAACTTAT TAGCTGTTTG GCAGCCTTCC GGCAGTTTTG GAATGGACTT TCTCAGGAGT 
     1201 CTCATGAACA GTGTGTCCAG TGGATTGTTA AATTTATTCA CGGCCAACAT AGCCCGAAAA 
     1261 GGATTTCCTT TCTCTATGAC TGCTTAGCCA TGGCAGTTGA GACTGGTCTT CTTCCACCCA 
     1321 GGATGGTTTG TGAATCTTTG ATAAACTCTG ACACTCTTGA ATGGGAAAGA ACACAGCTTT 
     1381 GGGCCTTAAC ATTTAAGCTG GTTCGGAAAA TCATTGGAGG AGTGGATTAT AAGGGTGTTC 
     1441 GAGACCTCCT GAAAGTGATT TTGGAGAAAA TCTTGACAAT CCCGAATACT GTAAGCTCTG 
     1501 CTGTTGTCCA GCAGCTTCTG GCAGCAAGAG AGGTTATAGC GTATATCTTG GAACGAAATG 
     1561 CCTGCCTGTT GCCAGCCTAC TTTGCAGTCA CAGAGATCAG AAAATTATAT CCCGAGGGAA 
     1621 AACTTCCACA CTGGTTACTA GGAAACTTGG TATCAGACTT TGTGGATACC TTCAGGCCCA 
     1681 CAGCGAGGAT AAACTCCATT TGTGGCCGTT GCAGTCTTCT GCCAGTTGTG AACAACTCGG 
     1741 GAGCCATCTG TAACTCATGG AAACTGGACC CTGCAACTCT CCGCTTTCCC TTGAAAGGCC 
     1801 TTTTGCCATA CGATAAGGAT CTGTTTGAAC CCCAGACTGC TTTGTTGAGA TATGTACTGG 
     1861 AGCAGCCTTA CTCCAGGGAT ATGGTCTGCA ATATGCTAGG TTTAAACAAG CAGCACAAGC 
     1921 AGCGCTGCCC TGTGCTGGAG GACCAGTTGG TGGACCTGGT GGTGTACGCC ATGGAGAGGT 
     1981 CTGAGACCGA GGAGAAGTTT GACGATGGGG GAACCAGCCA GCTCCTGTGG CAGCACCTTT 
     2041 CCAGCCAGCT CATTTTCTTT GTGCTTTTCC AGTTTGCCAG TTTCCCACAT ATGGTTCTGT 
     2101 CTCTCCACCA GAAGTTAGCA GGGCGAGGAC TGATTAAAGG GCGAGACCAT CTGATGTGGG 
     2161 TTCTGCTGCA GTTCATCTCG GGAAGTATTC AGAAAAATGC GCTTGCTGAC TTCCTCCCTG 
     2221 TCATGAAGCT CTTTGACCTG CTGTACCCAG AGAAGGAATG TATCCCAGTT CCTGATATTA 
     2281 ACAAACCACA GTCAACACAT GCCTTTGCAA TGACTTGTAT TTGGATTCAT CTTAATAGAA 
     2341 AAGCTCAAAA TGACAACTCC AAGCTGCAGA TCCCCATACC CCACTCCCTG AAGCTGCACC 
     2401 ACGAGTTCCT ACAGCAGAGT CTGAGAAACA AAAGTTTGCA GATGAATGAC TATAAGATCG 
     2461 CCCTGCTGTG TAATGCGTAT TCTACAAATT CAGAGTGTTT CACCTTACCT ATGGGCGCTC 
     2521 TGGTAGAGAC CATTTATGGC AATGGAATTA TGAGAGTGCC TCTCCCCGGG ACAAGCTGTC 
     2581 TGGCCTCAGC ATCAGTAACT CCCTTACCAA TGAACCTCCT GGATTCTCTG ACAGTGCATG 
     2641 CCAAGATGAG CCTTATTCAC AGCATTGCAA CCAGAGTGAT AAAACTGGCT CATACAAAAT 
     2701 CCAGTGTCGC ATTGGCTCCA GCCCTGGTGG AAACTTACAG TCGTTTACTG GTCTACATGG 
     2761 AAATAGAATC TTTGGGCATC AAAGGATTTA TCAGTCAGCT CCTGCCCACT GTCTTCAAGT 
     2821 CCCATGCCTG GGGCATCCTG CACACGCTGC TGGAGATGTT CAGCCACCGG ATGCACCACA 
     2881 TTCAGCCACA TTACCGAGTT CAGCTCCTGA GCCATCTCCA CACCCTGGCT GCAGTTGCAC 
     2941 AAACCAACCA GAACCAGCTC CATCTGTGCG TAGAAAGTAC TGCGCTGAGG CTCATCACGG 
     3001 CGCTGGGGAG CTCCGAGGTC CAGCCGCAGT TTACACGCTT CCTCAACGAT CCCAAAACAG 
     3061 TGCTGTCCGC AGAATCTGAA GAGCTGAACC GAGCCTTGAT CCTGACCTTG GCCAGAGCAA 
     3121 CCCACGTCAC AGATTTTTTT ACAGGATCTG ATTCAATACA GGGAACTTGG TGTAAAGATA 
     3181 TCCTTCAGAC CATCATGACT TTTACTCCTC ATAACTGGGC TTCGCACACC CTTAGCTGTT 
     3241 TTCCAGCCCC TCTGCAGGCA TTCTTCAAGC AAAATAATGT ACCTCAGGAA AGCCGTTTTA 
     3301 ATCTGAAAAA AAACGTGGAA GAAGAGTACA GGAAGTGGAA GTCAATGACT GATGAAAACG 
     3361 AGATCATCAC CCAGTTCTCC ATGCAAGGCT TCCCTCCGCT CTTCCTCTGT CTCCTCTGGA 
     3421 AAATGCTCCT GGAAACGGAT CACATTAGTC AGATTGGGTA CAAAGTACTA GAGAGAATTG 
     3481 GGGCCAGGGC CTTGGTGGCC CACGTGAGAA CCTTTGCAGA TTTCTTGGTG TATGAGTTCT 
     3541 CTACATCAGC AGGAGGTCAG CAACTCAATA AATGCATTGA GATTCTCAAT GACATGGTGT 
     3601 GGAAGTACAA CATTGTCACA CTGGACAGAC TAATTCTCTG CCTGGCTATG CGTAGTCATG 
     3661 AAGGAAATGA AGCTCAGGTT TGTTATTTCA TAATTCAGTT GCTATTGCTA AAACCAAATG 
     3721 ACTTCCGAAA CCGAGTGAGT GACTTTGTGA AGGAGAACTC CCCAGAGCAC TGGCTGCAGA 
     3781 GCGACTGGCA CACCAAACAC ATGAGCTACC ACAAGAAATA TCCAGAGAAG TTGTACTTTG 
     3841 AAGGCCTGGC AGAACAGGTT GACCCTCCTG TACCAATTCA GTCCCCGTAT CTGCCCATCT 
     3901 ACTTTGGAAA CGTGTGTCTC CGGTTTCTTC CAGTATTTGA TATAGTGATC CACAGGTTTT 
     3961 TAGAGTTGCT GCCAGTGTCC AAATCACTAG AGACTCTCCT GGATCACCTA GGGGGCTTGT 
     4021 ACAAATTCCA CGACCGGCCG GTGACTTACC TGTATAACAC TTTACACTAT TATGAAATGT 
     4081 GCCTGAGAAA CCGTGACCAT CTCAAACGAA AACTCGTCCA TGCCATCATT GGCTCCCTCA 
     4141 AAGATAACCG GCCACAGGGC TGGTGTCTCA GTGACACCTA CCTGAAGCAT GCTATGAATG 
     4201 CACGGGAGGA CAACCCCTGG GTTCCAGAGG ACTCCTACTA CTGCAAGCTG ATTGGCCGGC 
     4261 TGGTAGACAC CATGGCTGGC AAGTCTCCGG GACCCTTCCC AAACTGTGAC TGGAGATTCA 
     4321 ATGAGTTCCC CAACCCTGCT GCCCATGCTC TGCATGTGAC GTGTGTGGAA CTCATGGCCT 
     4381 TGGCAGTTCC AGGCAAAGAT GTCGGGAATG CCCTTCTCAA TGTTGTCCTG AAAAGCCAGC 
     4441 CCTTAGTGCC AAGGGAGAAC ATCACAGCAT GGATGAATGC AATTGGTCTG ATCATCACGG 
     4501 CCCTCCCAGA ACCATACTGG ATTGTGCTTC ATGACCGAAT CGTGAGTGTA ATCAGCAGTT 
     4561 CTAGCCTGAC GTCAGAGACA GAGTGGGTTG GTTATCCGTT CCGCCTCTTC GACTTCACCG 
     4621 CTTGTCACCA GTCCTACTCT GAGATGAGCT GCAGCTACAC GTTAGCGCTG GCTCACGCCG 
     4681 TGTGGCACCA CTCCAGCATC GGACAGCTCT CGCTTATCCC GAAGTTTCTC ACTGAAGTGC 
     4741 TTCTTCCCGT GGTGAAGACT GAATTCCAGT TGCTTTACGT GTACCATCTC GTAGGGCCAT 
     4801 TTTTACAGCG ATTCCAGCAG GAGAGAACCC GGTGCATGAT CGAGATTGGA GTGGCATTTT 
     4861 ATGACATGCT GCTGAATGTA GACCAGTGCA GCACCCACCT CAATTACATG GACCCCATCT 
     4921 GCGACTTCCT ATACCATATG AAGTATATGT TCACTGGAGA CAGTGTGAAG GAGCAGGTAG 
     4981 AGAAGATCAT CTGTAATTTA AAGCCAGCTT TGAAGCTTCG TCTTCGGTTC ATCACGCACA 
     5041 TCAGCAAGAT GGAGCCGGCA GTGCCACCCC AGGCCCTCAG CAGCGGGTCT CCAGCCCCTC 
     5101 AGGCCAACCA GGTGCCCACA GCTCTGCCCG TGACTCAGAC GCGTACGCGG CCGCTCGAGC 
     5161 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5221 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5281 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5341 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5401 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5461 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5521 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5581 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5641 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5701 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5761 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5821 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5881 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5941 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6001 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6061 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6121 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6181 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6241 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6301 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6361 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6421 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6481 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6541 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6601 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6661 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6721 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6781 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6841 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6901 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6961 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7021 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7081 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7141 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7201 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7261 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7321 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7381 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7441 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7501 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7561 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7621 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7681 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7741 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7801 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7861 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7921 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7981 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8041 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8101 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8161 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8221 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8281 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8341 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8401 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8461 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8521 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8581 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8641 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8701 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8761 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8821 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8881 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8941 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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