LOCUS       RR217601                8988 bp    DNA     circular  SYN 
DEFINITION  Sik3 (myc-DDK-tagged) - Rat SIK family kinase 3 (Sik3)
ACCESSION   NM_001271216
VERSION     NM_001271216.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5145..5236 
                     /label="Myc-DDK"  
     misc_feature    5266..5851 
                     /label="PolyA signal"  
     misc_feature    5323..5342 
                     /label="Restriction Enzyme"  
     misc_feature    6127..6799 
                     /label="ColE1"  
     misc_feature    7229..8023 
                     /label="Kan/Neo"  
     misc_feature    8058..8360 
                     /label="SV40 ori"  
     misc_feature    8446..8923 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5127..5133 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5127 
                     /label="Sik3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGCG GCGGCGAGCG GAGCTGGCGG GGTTGCCGTG GCCGGAGCAG 
     1081 GGGGAGCCGG GCCGGCCGGT CGCCTGCTGC CTCCGCCCGC CGCGGGGCCC CCGGCAGCCC 
     1141 CCGCCGCAGT GCCCCCGGCC GCCTGCCCGC CGCGTCCCAC AGCCCCGGCC TCCCGCGGGT 
     1201 CGATGGCCGC TCGCATCGGC TACTACGAGA TCGACCGCAC CATCGGCAAG GGCAACTTCG 
     1261 CTGTGGTCAA GCGGGCCACG CACCTCGTCA CCAAGGCCAA GGTTGCTATT AAAATCATAG 
     1321 ATAAGACCCA GTTGGATGAA GAGAACCTGA AGAAGATTTT CCGGGAGGTT CAGATAATGA 
     1381 AGATGCTTTG CCACCCACAT ATCATCAGGC TCTACCAGGT CATGGAGACA GAGCGCATGA 
     1441 TTTACCTGGT GACAGAATAC GCTAGCGGAG GGGAGATATT TGACCACTTG GTGGCCCATG 
     1501 GAAGAATGGC GGAGAAGGAA GCTCGGCGGA AGTTCAAACA GATTGTCACA GCTGTATATT 
     1561 TTTGTCACTG TCGGAATATC GTTCATCGTG ATTTAAAAGC TGAAAATTTG CTTCTGGACG 
     1621 CCAATCTGAA TATCAAAATA GCAGACTTTG GCTTCAGCAA CCTCTTCACT CCGGGTCAGC 
     1681 TGCTAAAGAC GTGGTGTGGC AGTCCTCCCT ATGCCGCACC AGAGCTCTTC GAAGGCAAGG 
     1741 AATATGATGG GCCCAAAGTG GACATATGGA GTCTTGGAGT TGTCCTCTAT GTGCTTGTGT 
     1801 GTGGCGCCCT GCCGTTTGAT GGGAGCACAC TGCAGAATCT GCGGGCCCGT GTGCTGAGCG 
     1861 GCAAGTTCCG CATCCCGTTC TTCATGTCCA CAGAGTGTGA GCACTTGATC CGCCACATGC 
     1921 TGGTGTTAGA TCCAAATAAG CGTCTCTCAA TGGAGCAGAT CTGCAGGCAC AGGTGGATGA 
     1981 AGCTCGGAGA TGCAGACCCC AACTTTGAGA GGTTAATAGC AGAATGCCAG CAGCTGAAGG 
     2041 AGGAAAGGCA GGCAGAGCCC CTCAATGATG AGGTTCTCTT GGCCATGGAA GACATGGGAC 
     2101 TAGACAAGGA ACGCACACTT CAGTCATTAA GATCGGATGC CTATGACCAC TATAGTGCGA 
     2161 TCTACAGCCT GCTGTGCGAT CGACACAAGA AAAACAAAAC TCTGCGTCCT GGAGCACTTC 
     2221 CCAGCACGCC CCAAGCCATG ACTTTTCAGG CACCAGTCAA TATCCAGGCG GAGCAGACAG 
     2281 GCACTGCTAT GAACATTAGC GTCCCCCAAG TTCAGCTGAT CAACCCAGAG AACCAAATTA 
     2341 TAGAGCCTGA CGGGGCCGTG AACTTGGACA GCGACGAGGG TGAAGAGCCT TCTCCAGAAG 
     2401 CCTTGGTTCG CTATTTGTCA ATGAGGAGGC ACACGGTGGG AGTGGCTGAC CCACGCACGG 
     2461 AAGTTATGGA CGAGATGCAG AAGCTCCTGC CCGGCTTCCC TGGAGCGAAC CCACAGACTC 
     2521 CATTCCTGCA GGTGGCCCCT AACATGAACT TCATGCACAA CCTGCTGCCC ATGCAGAGTC 
     2581 TGCAGCCAAC TGGGCAGCTT GAGTACAAGG AGCAGTCTCT ATTACAGCCG CCTACACTAC 
     2641 AGCTACTGAA TGGAATGGGG CCCCTTGGCC GAAGAGCCTC AGACGGAGGC GCCAACATCC 
     2701 AACTACATGC CCAGCAGCTG CTCAAGCGCC CACGGGGACC ATCCCCACTT GTCACCATGA 
     2761 CACCAGCAGT GCCAGCAGTT ACCCCTGTGG ATGAGGAGAG CTCGGATGGG GAGCCAGATC 
     2821 AGGAAGCTGT GCAGAGGTAC TTGGCAAATA GGTCCAAAAG GCACACACTG GCCATGACCA 
     2881 GCCCGACAGC TGAGATCCCA CCGGACCTGC AACGGCAGCT AGGACAACAG TCTTTCCGTT 
     2941 CCCGGGTCTG GCCTCCTCAC CTGGTACCTG ACCAGCATCG CTCCACCTAC AAGGACTCCA 
     3001 ACACCCTGCA TCTCCCTACG GAGCGTTTCT CCCCCGTGCG CCGGTTCTCA GACGGGGCGG 
     3061 CAAGCATCCA GGCCTTCAAA GCTCACCTGG AAAGAATGGG CAACAGCAGC AGCATCAAAC 
     3121 AGTTACAACA GGAGTGTGAG CAGCTACAGA AGATGTACGG CGGGCAGGTT GACGAGAGAA 
     3181 CCCTGGAGAA GACCCAGCAG CAGCATATGC TGTACCAGCA GGAGCAGCAC CATCAGATCC 
     3241 TCCAGCAGCA AATTCAAGAT TCTATTTGTC CTCCTCAGCC CTCCCCACCT CTTCAGGCTG 
     3301 CATGTGAAAA TCAGCCAACC CTCCTCACCC ACCAGCTGCA GAGGTTAAGG ATTCAACCTT 
     3361 CAAGCCCACC CCCCAACCAC CCCAGCAACC ATCTCTTCAG GCAGCCAAGT AACAGTCCTC 
     3421 CCCCAGTCAG CAGTGCCATG ATCTCTTCTC ACGGTGCTAC GTCTCCCTCC CAGTTTCAAG 
     3481 GCTTACCCTC CCATGGTGCA ATCTTCCAGC AGCAACCTGA GAACTGTTCC CCACCTCCCA 
     3541 GTGTAGCACT AACCTGCTTG GGCCTACAGC AGGCTAGCCA GTCACAGCCA GTGACTATCC 
     3601 AGCTACAGGA GCCAGTTGAC ATGCTCAGCA ACATGGCTGG GACAACTGCA GGTTCTGCAG 
     3661 GGCGGGGTAT CCCCATCAGC CCCAGTGCCA GTCAGATTCA GATGCAGCAC CGTACCAGCC 
     3721 TAATGGCTCC CTTCGGCTAT GGGCACCGGC CCTTGTCCAA GCAGCTGAGT GCTGACAGCG 
     3781 CAGAGGCTCA CAGCTTGAGC ATGAATCGAT TCTCCCCTGC CAACTACGAC CAGGCGCATT 
     3841 TACACCCCCA TCTGTTCTCG GAGCAGTCCC GAGGGCCCCC CAGCAGCTAC AGCCCTTCAA 
     3901 CAGGAGTGGG GTTTCCTCCG GCTCAAGCCC TGAGAGTTCC TCCGCTTGAC CAGTTCCCCA 
     3961 CTTTCCCTCC CAGCGCCCAT CAGCAGCCAC ACTATACCAC ATCAGCACTA CAGCAGGCCC 
     4021 TGCTGTCCCC TACACCACCA GACTATACCA GACACCAGCA GGTTCCCCAC ATCCTTCAAG 
     4081 GACTGCTCTC TCCCCGGCAT TCACTCACCG GCCACTCGGA CATTCGGCTG CCTCCAGCAG 
     4141 AGTTTGCCCA GCTCATCAAA AGACAGCAGC AGCATCGACA GCAGCAGCAA CAGCAGCAGC 
     4201 AGCAGCAGCA GCAAGAATAC CATGAGCTGT TCAGGCACAT GAACCAAGGG GATGCTGTTA 
     4261 GCCTAGCTCC CGGCCTCGGG GGACAGAATA TGACAGAGCA CCAGGCTTTA TCTTATCAAA 
     4321 ATGCTGACTC ATACCACCGC CACCACACCA GCCCCCAGCA TCTCCTACAG ATCAGAGCGC 
     4381 AAGATTGTAT CTCACAGGGT CCCTTGCCCA CCCCCACCCG TGGGTATGCC CGTCAGTCAC 
     4441 CACTAATGCA TTCGGAGAGT ATGGAAGAAG ACTGCTTGTG TGAAGGGGTC AAGGAGGGCT 
     4501 TCGCGGACAA AAGCTCAAGC ACACTGACCA AAGGTTGCCA TGACAGCCCT CTGCTCTTGT 
     4561 GTACCAGTGG GCCTGGGGAC CCCGAGCCTT TGCTGGGAAC TGTGAGTCAG GCCCGGGAAC 
     4621 TGGGAATCCA TCCCTACGGA CACCAGCCAA CGGCCACCAC ATTCAGTAGA AATAAGGTGC 
     4681 CCAGCCGAGA GTCTGTCATA GGGAACTGCA TGGAGAGAAG TTCTCCTGGA CAAGCAATGG 
     4741 AGCTGCCGGA CCACAACGGC CTTGGGTATC CTGCACGGCC CTTAGTCAGT GAGCACCTCA 
     4801 GGTCCCGGAC CCTCCAGAGA CACCACACCA TCCAGAACAG CGACGATGCT TATGTACAGC 
     4861 TGGATACCTT GCCAGGAATG AGCCTGGTGG CAGGAAAGGC ACTGAGCTCT GCCCGGATGT 
     4921 CAGATGCAGT TCTTAGTCAG TCTTCACTCA TGGGCAGCCA GCAGTTTCAG GATGAGGAAG 
     4981 ATGAAGAATG TGGGGTGAGC CTGGGCCATG AGCACCCAGC CCTGGGTGAC GGTAGCCAGC 
     5041 ATTTAAACTC CCGCTATCCA GCTACGTGTG TTACAGACAT CATGCTCAAC CACAAGCATC 
     5101 CAGAGGTCTC CTTCAGCATG GAGCAGGCTG GCGTGACGCG TACGCGGCCG CTCGAGCAGA 
     5161 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5221 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5281 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5341 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5401 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5461 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5521 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5581 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5641 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5701 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5761 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5821 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5881 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5941 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     6001 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     6061 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     6121 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6181 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6241 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6301 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6361 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6421 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6481 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6541 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6601 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6661 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6721 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6781 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6841 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6901 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6961 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     7021 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     7081 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     7141 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7201 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7261 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7321 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7381 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7441 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7501 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7561 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7621 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7681 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7741 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7801 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7861 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7921 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7981 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     8041 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     8101 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     8161 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8221 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8281 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8341 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8401 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8461 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8521 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8581 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8641 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8701 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8761 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8821 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8881 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8941 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//