LOCUS       RR217599                8943 bp    DNA     circular  SYN 
DEFINITION  Astn2 (myc-DDK-tagged) - Rat astrotactin 2 (Astn2)
ACCESSION   NM_001271363
VERSION     NM_001271363.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5100..5191 
                     /label="Myc-DDK"  
     misc_feature    5221..5806 
                     /label="PolyA signal"  
     misc_feature    5278..5297 
                     /label="Restriction Enzyme"  
     misc_feature    6082..6754 
                     /label="ColE1"  
     misc_feature    7184..7978 
                     /label="Kan/Neo"  
     misc_feature    8013..8315 
                     /label="SV40 ori"  
     misc_feature    8401..8878 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5082..5088 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5082 
                     /label="Astn2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGCCGCC GGCGCCCGGC GCAGCCCGGG CCCCGGCTTG GGGCTTCGGG 
     1081 GGCGGCCGAG GCTCCGCTTC CACCCGGGGC CGCCGCCGCC ACCGCCACTG CCGCTGCTGT 
     1141 TGCTGTTTCT GCTCTTGCTG CCGCCGCCGC CGTTGCTGGC CGGCGCCACC GCCGCCGCCG 
     1201 CCTCGCGGGA GCCCGACAGC CCATGCCGGC TCAAGACTGT CACGGTATCC ACGCTGCCCG 
     1261 CTCTGCGGGA AAGCGACATC GGCTGGAGCG GTGCCCGCGC CGGGGCCGCG GCCGGGGCTG 
     1321 GGGCCGGGAC CGGAGCCGGG GCCGGAGCTT CTGCTGCTGC CGCCGCTGCT GCCGCCGCGT 
     1381 CCCCGGGCTC CGCGGGTTCA GCCGGTACAG CCGCGGAGTC TCGTCTCCTG CTCTTTGTGC 
     1441 GTAACGAGCT CCCTGGGCGC ATCGCGGTAC AAGATGACCT GGACAACACC GAGCTGCCCT 
     1501 TCTTCACCCT GGAGATGTCT GGCACAGCAG CGGACATTTC GTTGGTTCAC TGGAGACAGC 
     1561 AGTGGCTGGA GAATGGCACA CTGTACTTCC ATGTTTCCAT GAGCAGCTCT GGGCAGCTGG 
     1621 CCCAGGCCAC TGCTCCCACA CTGCAGGAGC CCTCAGAAAT CGTTGAAGAA CAGCTGCATA 
     1681 TCCTCCACAT CTCTGTGATG GGTGGACTCA TTGCACTGCT CCTTTTGCTC CTGGTGTTCA 
     1741 CAGTGGCATT GTATGCCCAA CGGCGCTGGC AGAAGCGCAG GCGCATCCCA CAGAAGAGCG 
     1801 CAAGCACAGA AGCCACTCAT GAGATTCACT ACATCCCATC TGTGCTGCTC GGTCCTCAGG 
     1861 CCAGGGAGAG CTTCCGCTCC ACCAGACTAC AGGCACACAA CTCAGTCATC GGTGTGCCTA 
     1921 TCCGGGAAAC CCCCATCCTA GATGACTATG ACTATGAAGA GGAAGAGGAC CCACCCAGGC 
     1981 GGGCCAACCA TGTCTCCCGT GAGGATGAGT TTGATAGCCA GATGACCCAT GCCCTGGACA 
     2041 GCTTGGGAAG GCCAGGAGAA GAGAAAGTGG AATTTGAGAA GAAAGCAGCA GCTGAGGCGA 
     2101 CACAGGAGAC AGTGGAGTCC CTGATGCAGA AGTTCAAGGA GAGTTTCCGA GCTAACACAC 
     2161 CTGTGGAGAT CGGTCAATTG CAGCCAGCCT CGCGCAGCAG CACCTCTGCA GGGAAGAGGA 
     2221 AACGGAGGAA CAAGTCTCGA GGGGGAATCA GTTTTGGGAG AACCAAGGGA ACATCAGGCT 
     2281 CAGAAGCAGA TGACGAAACA CAGCTGACGT TCTACACAGA GCAGTACCGC AGTCGCCGCC 
     2341 GCAGCAAAGG TTTACTGAAA AGCCCTGTGA ACAAGACAGC CTTAACACTG ATTGCTGTCA 
     2401 GCTCCTGCAT TTTGGCCATG GTGTGTGGCA ACCAGATGTC CTGTCCACTT ACTGTGAAGG 
     2461 TGACTTTGCA TGTGCCGGAG CACTTCATTG CAGATGGAAG CAGCTTTGTG GTGAGTGAAG 
     2521 GAAGCTACCT GGATATCTCT GACTGGCTAA ACCCTGCCAA GCTGTCCTTG TACTACCAGA 
     2581 TCAATGCCAC CTCACCATGG GTGAGAGACC TCTGTGGACA GAGGACGACA GATGCCTGTG 
     2641 AACAGCTTTG TGACCCAGAA ACTGGGGAGT GCAGTTGTCA CGAAGGTTAC GCCCCTGACC 
     2701 CAGTCCACAG ACATCTATGT ATCCGAAGTG ACTGGGGTCA AAGTGAAGGA CCTTGGCCTT 
     2761 ACACAACACT GGAGAGGGGT TATGATCTGG TGACAGGAGA GCAAGCCCCT GAAAAGATTC 
     2821 TCAGGTCTAC TTTCAGCTTG GGCCAAGGCC TCTGGCTTCC GGTCAGCAAA AGCTTCGTGG 
     2881 TTCCACCGGT GGAGCTGTCC ATCAACCCCC TGGCAAGTTG CAAGACTGAT GTACTCGTCA 
     2941 CAGAAGACCC TGCAGATGTC AGGGAAGAAG CAATGCTGTC CACGTACTTT GAGACCATCA 
     3001 ATGACCTGCT CTCCTCCTTT GGTCCAGTCC GTGACTGCTC AAGGAACAAT GGGGGCTGCA 
     3061 CCCGCAACTT CAAGTGTGTG TCTGACCGCC AGGTGGACTC CTCGGGATGT GTGTGTCCTG 
     3121 AGGAACTGAA GCCCATGAAG GATGGTTCCG GCTGCTATGA TCACTCCAAG GGCATTGACT 
     3181 GCTCTGATGG CTTTAATGGA GGGTGTGAGC AGCTTTGCCT ACAACAAACA ATGCCTTTGC 
     3241 CCTATGATGC CACCTCCAGC ACCATCTTCA TGTTCTGCGG CTGTGTGGAG GAGTACAAGC 
     3301 TGGCTCCTGA TGGGAAATCC TGTCTAATGC TCTCGGATGT CTGTGACGGC CCCAAGTGCC 
     3361 TCAAATCTGA CTCCAAATTC AATGATACCC TCTTTGGAGA GATGCTACAT GGTTACAACA 
     3421 ACCGGACACA ACATGTAAAC CAAGGCCAAG TCTTCCAAAT GACATTCAGG GAGAACAACT 
     3481 TCATCAAGGA CTTTCCCCAG CTGGCGGATG GACTGTTGGT GATCCCGCTA CCAGTGGAAG 
     3541 AGCAATGCCG AGGGGTTCTC TCTGAGCCCC TCCCAGACCT TCAGCTTCTC ACGGGAGACA 
     3601 TCAGGTATGA CGAGGCCATG GGTTACCCCA TGGTGCAGCA GTGGCGTGTC CGGAGCAACC 
     3661 TCTATCGTGT GAAGCTGAGT ACCATCACCC TCTCAGCAGG CTTCACGAAT GTCCTAAAGA 
     3721 TCCTGACCAA AGAGAGCAGT CGGGATGAGC TGCTCTCCTT CATCCAGCAC TATGGCTCCC 
     3781 ACTACATTGC AGAGGCTCTG TATGGTTCTG AACTCACTTG CATCATCCAT TTTCCCAGCA 
     3841 AGAAGGTCCA GCAGCAGCTA TGGCTCCAGT ATCAGAAAGA GACCACGGAG CTGGGCAGCA 
     3901 AGAAGGAGCT CAAGTCCATG CCCTTCATCA CCTACCTCTC TGGTCTACTG ACAGCCCAGA 
     3961 TGCTATCAGA TGACCAACTC ATCTCAGGTG TGGAGATTCG ATGTGAGGAA AAGGGCCGCT 
     4021 GTCCATCTAC CTGTCACCTT TGCCGCCGGC CAGGCAAAGA ACAGCTGAGC CCCACACCAG 
     4081 TGCTGCTGGA GATTAACCGT GTGGTGCCAC TTTACACCCT CATCCAAGAC AATGGCACAA 
     4141 AGGAGGCCTT CAAGAATGCA CTGATGAGTT CTTACTGGTG TTCTGGTAAA GGAGATGTGA 
     4201 TCGATGACTG GTGCAGGTGT GACCTCAGTG CCTTCGATGC CAGTGGACTT CCCAATTGTA 
     4261 GCCCCCTTCC ACAGCCGGTG TTACGACTTT CTCCAACAGT GGAACCTTCC AGCACTGTGG 
     4321 TTTCCCTGGA GTGGATAGAT GTTCAGCCAG CCATTGGAAC CAAAGTTTCT GACTATATCC 
     4381 TGCAGCACAA GAAAGTGGAT GAATACACAG ACACTGACCT CTACACAGGA GAATTCCTGA 
     4441 GTTTTGCTGA TGACTTACTC TCTGGCCTGG GCACATCTTG TGTAGCTGCT GGTCGAAGCC 
     4501 ACGGAGAGGT CCCGGAAGTC AGTATCTACT CGGTGATCTT CAAGTGCCTG GAGCCAGATG 
     4561 GCCTTTACAA GTTCACTCTG TATGCCGTGG ATACTCGAGG GAGGCACTCA GAGCTCAGCA 
     4621 CAGTGACTCT AAGAACAGCT TGTCCACTGG TAGATGACAA CAAAGCAGAA GAGATAGCTG 
     4681 ACAAGATTTA CAATCTATAC AATGGTTACA CCAGTGGCAA GGAACAGCAG ACTGCTTACA 
     4741 ACACACTGAT GGAAGTCTCT GCCTCCATGC TCTTCCGAGT CCAGCACCAC TACAACTCTC 
     4801 ACTATGAAAA GTTTGGTGAT TTCGTCTGGA GGAGTGAGGA TGAGCTGGGA CCGAGGAAGG 
     4861 CTCATTTGAT TCTGCGGCGG CTGGAGAGGG TCAGCAGCCA TTGTTCTAGT CTCTTGAGAA 
     4921 GCGCCTACAT CCAGAGTCGT GTGGATACCA TACCCTATCT CTTCTGCCGC AGTGAGGAGG 
     4981 TCCGGCCTGC AGGAATGGTG TGGTACAGCA TCCTCAAAGA CACCAAAATC ACATGTGAGG 
     5041 AGAAGATGGT GTCCATGGCC CGAAACACGT ATGGGGAAAC CAAAGGCCGG ACGCGTACGC 
     5101 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5161 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5221 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5281 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5341 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5401 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5461 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5521 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5581 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5641 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5701 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5761 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5821 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5881 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5941 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6001 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6061 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6121 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6181 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6241 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6301 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6361 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6421 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6481 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6541 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6601 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6661 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6721 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6781 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6841 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6901 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6961 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7021 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7081 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7141 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7201 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7261 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7321 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7381 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7441 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7501 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7561 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7621 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7681 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7741 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7801 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7861 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7921 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7981 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8041 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8101 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8161 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8221 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8281 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8341 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8401 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8461 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8521 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8581 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8641 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8701 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8761 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8821 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8881 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8941 TTT
//